{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T14:16:49Z","timestamp":1791728209690,"version":"4.3.4"},"reference-count":38,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2022,2,19]],"date-time":"2022-02-19T00:00:00Z","timestamp":1645228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>The potential of soft-shelled turtle peptides (STP) against fatigue was evaluated. Mice orally supplemented with STP significantly increased the swimming time until tiredness by 35.4\u201357.1%. Although not statistically significant, STP increased muscle and thymus mass. In addition, the serum lactate, ammonia, blood urea nitrogen content and creatine kinase activity in STP-fed mice were dramatically decreased when compared to the control group. Furthermore, STP supplementation increased the reserves of liver glycogen and muscle glycogen, thus improved the energy metabolism system of mice. STP treatment contributed to increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities as well as a decrease in malondialdehyde (MDA), indicating an improvement in oxidative stress protection. The Western blot (WB) results indicated that the STP supplement effectively altered the expression of oxidative stress-related protein by modulating the NRF2\/KEAP1 pathway. In summary, STP affected NRF2\/KEAP1 levels in skeletal muscle, leading to antioxidant activity and a slower time to exhaustion during exercise.<\/jats:p>","DOI":"10.3390\/foods11040600","type":"journal-article","created":{"date-parts":[[2022,2,21]],"date-time":"2022-02-21T08:18:05Z","timestamp":1645431485000},"page":"600","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Soft-Shelled Turtle Peptide Supplementation Modifies Energy Metabolism and Oxidative Stress, Enhances Exercise Endurance, and Decreases Physical Fatigue in Mice"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2344-861X","authenticated-orcid":false,"given":"Hao","family":"Zhong","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyuan","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3599-2682","authenticated-orcid":false,"given":"Junhui","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yipeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Yu","sequence":"additional","affiliation":[{"name":"Yuyao Lengjiang Turtle Industry, Ningbo 315400, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongfa","family":"Guan","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9770-1721","authenticated-orcid":false,"given":"Fengqin","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.nut.2008.03.001","article-title":"Effects of (\u2212)-epigallocatechin gallate in liver of an animal model of combined (physical and mental) fatigue","volume":"24","author":"Tanaka","year":"2008","journal-title":"Nutrition"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Yuan, T., Wu, D., Sun, K., Tan, X., Wang, J., Zhao, T., Ren, B., Zhao, B., Liu, Z., and Liu, X. (2019). Anti-Fatigue activity of aqueous extracts of Sonchus arvensis L. in exercise trained mice. Molecules, 24.","DOI":"10.3390\/molecules24061168"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1016\/j.peptides.2008.03.004","article-title":"The decapeptide CMS001 enhances swimming endurance in mice","volume":"29","author":"Wang","year":"2008","journal-title":"Peptides"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.foodchem.2010.06.007","article-title":"In vitro antioxidant activity and in vivo anti-fatigue effect of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion","volume":"124","author":"You","year":"2011","journal-title":"Food Chem."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1080\/10408690490468489","article-title":"Antioxidants and prevention of chronic disease","volume":"44","author":"Willcox","year":"2004","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1016\/j.ijbiomac.2021.03.018","article-title":"Synthesis, stability and anti-fatigue activity of selenium nanoparticles stabilized by Lycium barbarum polysaccharides","volume":"179","author":"Liu","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.2105\/AJPH.87.9.1449","article-title":"The prevalence and morbidity of chronic fatigue and chronic fatigue syndrome: A prospective primary care study","volume":"87","author":"Wessely","year":"1997","journal-title":"Am. J. Public Health"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12875-016-0493-0","article-title":"Investigating unexplained fatigue in general practice with a particular focus on CFS\/ME","volume":"17","author":"Bansal","year":"2016","journal-title":"BMC Fam. Pract."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1093\/eurpub\/ckp113","article-title":"Fatigue and chronic fatigue syndrome-like complaints in the general population","volume":"20","author":"Zielhuis","year":"2010","journal-title":"Eur. J. Public Health"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1016\/j.carbpol.2012.10.023","article-title":"Effects of oat \u03b2-glucan on endurance exercise and its anti-fatigue properties in trained rats","volume":"92","author":"Xu","year":"2013","journal-title":"Carbohyd. Polym."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.3390\/md11061800","article-title":"A New Antiproliferative and Antioxidant Peptide Isolated from Arca subcrenata","volume":"11","author":"Chen","year":"2013","journal-title":"Mar. Drugs"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2174\/0929866523666160802155347","article-title":"Marine-derived bioactive peptides for biomedical cectors: A review","volume":"24","author":"Iqbal","year":"2017","journal-title":"Protein Pept. Lett."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Khotimchenko, Y. (2018). Pharmacological Potential of Sea Cucumbers. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19051342"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.dci.2010.10.002","article-title":"Cod cathelicidin: Isolation of the mature peptide, cleavage site characterisation and developmental expression","volume":"35","author":"Broekman","year":"2011","journal-title":"Dev. Comp. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Yang, X.R., Zhang, L., Ding, D.G., Chi, C.F., Wang, B., and Huo, J.C. (2019). Preparation, identification, and activity evaluation of eight antioxidant peptides from protein hydrolysate of Hairtail (Trichiurus japonicas) muscle. Mar. Drugs, 17.","DOI":"10.3390\/md17010023"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1080\/02652048.2016.1194904","article-title":"Separation and nanoencapsulation of antitumor peptides from Chinese three-striped box turtle (Cuora trifasciata)","volume":"33","author":"He","year":"2016","journal-title":"J. Microencapsul."},{"key":"ref_17","first-page":"25","article-title":"Studies on the antioxidant activity of soft-shelled turtle enzymatic hydrolysates","volume":"4","author":"Xu","year":"2007","journal-title":"J. Chin. Inst. Food Sci. Technol."},{"key":"ref_18","first-page":"3109","article-title":"Soft-shelled turtle peptide modulates microRNA profile in human gastric cancer AGS cells","volume":"15","author":"Wu","year":"2018","journal-title":"Oncol. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"104207","DOI":"10.1016\/j.jff.2020.104207","article-title":"Anti-fatigue activities of hairtail (Trichiurus lepturus) hydrolysate in an endurance swimming mice model","volume":"74","author":"Wang","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2113","DOI":"10.1039\/C4FO00121D","article-title":"Effect and mechanism of mackerel (Pneumatophorus japonicus) peptides for anti-fatigue","volume":"5","author":"Wang","year":"2014","journal-title":"Food Funct."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4770","DOI":"10.3390\/ijms12084770","article-title":"Anti-fatigue properties of tartary buckwheat extracts in mice","volume":"12","author":"Jin","year":"2011","journal-title":"Int. J. Mol. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"4266","DOI":"10.1039\/C7FO01658A","article-title":"Anti-fatigue effects of small-molecule oligopeptides isolated from Panax quinquefolium L. in mice","volume":"9","author":"Li","year":"2018","journal-title":"Food Funct."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zhang, J.B., Zhao, Y.Q., Wang, Y.M., Chi, C.F., and Wang, B. (2019). Eight collagen peptides from hydrolysate fraction of Spanish mackerel skins: Isolation, identification, and in vitro antioxidant activity evaluation. Mar. Drugs, 17.","DOI":"10.3390\/md17040224"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4604387","DOI":"10.1155\/2020\/4604387","article-title":"Sea cucumber peptides improved the mitochondrial capacity of mice: A potential mechanism to enhance gluconeogenesis and fat catabolism during exercise for improved antifatigue property","volume":"2020","author":"Yu","year":"2020","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"104677","DOI":"10.1016\/j.jff.2021.104677","article-title":"High-degree hydrolysis sea cucumber peptides improve exercise performance and exert antifatigue effect via activating the NRF2 and AMPK signaling pathways in mice","volume":"86","author":"Wang","year":"2021","journal-title":"J. Funct. Foods"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"103725","DOI":"10.1016\/j.jff.2019.103725","article-title":"Anti-fatigue activity of purified anthocyanins prepared from purple passion fruit (P. edulis Sim) epicarp in mice","volume":"65","author":"Hu","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7755","DOI":"10.1039\/C9FO01182J","article-title":"Anti-fatigue effect of anwulignan via the NRF2 and PGC-1\u03b1 signaling pathway in mice","volume":"10","author":"Zhang","year":"2019","journal-title":"Food Funct."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"9192","DOI":"10.1039\/D0FO00334D","article-title":"Probiotic-fermented blueberry juice prevents obesity and hyperglycemia in high fat diet-fed mice in association with modulating the gut microbiota","volume":"11","author":"Zhong","year":"2020","journal-title":"Food Funct."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4405","DOI":"10.1113\/jphysiol.2013.251629","article-title":"Muscle glycogen stores and fatigue","volume":"591","author":"Westerblad","year":"2013","journal-title":"J. Physiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"100028","DOI":"10.1016\/j.crphar.2021.100028","article-title":"The potential roles of Nrf2\/Keap1 signaling in anticancer drug interactions","volume":"2","author":"Wang","year":"2021","journal-title":"Curr. Res. Pharmacol. Drug Discov."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1038\/s41573-018-0008-x","article-title":"Therapeutic targeting of the NRF2 and KEAP1 partnership in chronic diseases","volume":"18","author":"Cuadrado","year":"2019","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.procbio.2019.12.022","article-title":"Involvement of Nrf2 and Keap1 in the activation of antioxidant responsive element (ARE) by chemopreventive agent peptides from soft-shelled turtle","volume":"92","author":"Wang","year":"2020","journal-title":"Process Biochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1038\/s42255-020-0243-4","article-title":"Lactate: The ugly duckling of energy metabolism","volume":"2","author":"Rabinowitz","year":"2020","journal-title":"Nat. Metab."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1701043","DOI":"10.1002\/mnfr.201701043","article-title":"L-Citrulline supplementation-increased skeletal muscle PGC-1\u03b1 expression is associated with exercise performance and increased skeletal muscle weight","volume":"62","author":"Villareal","year":"2018","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Guo, Z., Lin, D., Guo, J., Zhang, Y., and Zheng, B. (2017). In vitro antioxidant activity and in vivo anti-fatigue effect of sea horse (Hippocampus) peptides. Molecules, 22.","DOI":"10.3390\/molecules22030482"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1038\/s42255-020-0251-4","article-title":"Skeletal muscle energy metabolism during exercise","volume":"2","author":"Hargreaves","year":"2020","journal-title":"Nat. Metab."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1080\/13510002.2017.1416773","article-title":"A systematic review of p53 regulation of oxidative stress in skeletal muscle","volume":"23","author":"Beyfuss","year":"2018","journal-title":"Redox Rep."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1039\/C9FO01414D","article-title":"Bioactive peptides derived from crimson snapper and in vivo anti-aging effects on fat diet-induced high fat Drosophila melanogaster","volume":"11","author":"Chen","year":"2020","journal-title":"Food Funct."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/4\/600\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:23:12Z","timestamp":1760134992000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/4\/600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,19]]},"references-count":38,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["foods11040600"],"URL":"https:\/\/doi.org\/10.3390\/foods11040600","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,19]]}}}