{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:54:05Z","timestamp":1760151245344,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,25]],"date-time":"2022-02-25T00:00:00Z","timestamp":1645747200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008781","name":"Pomeranian Medical University","doi-asserted-by":"publisher","award":["FSN-167-03\/17"],"award-info":[{"award-number":["FSN-167-03\/17"]}],"id":[{"id":"10.13039\/501100008781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>The intestinal wall and epithelial cells are interconnected by numerous intercellular junctions. Colostrum (Col), in its natural form, is a secretion of the mammary gland of mammals at the end of pregnancy and up to 72 h after birth. Recently, it has been used as a biologically active dietary supplement with a high content of lactoferrin (Lf). Lf, a glycoprotein with a broad spectrum of activity, is becoming more popular in health-promoting supplements. This study aims to investigate whether Col supplementation can affect small and large intestine morphology by modulating the expression of selected proteins involved in tissue integrity. We examined the thickness of the epithelium, and the length of the microvilli, and assessed the expression of CDH1, CDH2, CTNNB, CX43, VCL, OCLN, HP, MYH9, and ACTG2 gene levels using qRT-PCR and at the protein level using IHC. Additionally, to evaluate whether the effect of Col supplementation is temporary or persistent, we performed all analyses on tissues collected from animals receiving Col for 1, 3, or 6 months. We noticed a decrease in CDH1 and CDH2 expression, especially after 3 months of supplementation in the large intestine and in CTNNB in the small intestine as well as increased levels of CX43 and CTNNB1 in the small intestine. The present data indicate that Col can temporarily alter some components of the cell adhesion molecules involved in the formation of the cellular barrier.<\/jats:p>","DOI":"10.3390\/foods11050685","type":"journal-article","created":{"date-parts":[[2022,2,27]],"date-time":"2022-02-27T20:46:17Z","timestamp":1645994777000},"page":"685","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Colostrum-Induced Temporary Changes in the Expression of Proteins Regulating the Epithelial Barrier Function in the Intestine"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2157-0315","authenticated-orcid":false,"given":"Sylwia","family":"Rzeszotek","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Pomeranian Medical University in Szczecin, 72 Powsta\u0144c\u00f3w Wlkp., 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3900-4901","authenticated-orcid":false,"given":"Grzegorz","family":"Trybek","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pomeranian Medical University in Szczecin, 72 Powsta\u0144c\u00f3w Wlkp., 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3869-5077","authenticated-orcid":false,"given":"Maciej","family":"Tarnowski","sequence":"additional","affiliation":[{"name":"Department of Physiology, Pomeranian Medical University in Szczecin, 72 Powsta\u0144c\u00f3w Wlkp., 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1071-8337","authenticated-orcid":false,"given":"Karol","family":"Serwin","sequence":"additional","affiliation":[{"name":"Department of Infectious Tropical Diseases and Immune Deficiency, Pomeranian Medical University in Szczecin, Arko\u0144ska 4, 71-455 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7727-6388","authenticated-orcid":false,"given":"Aleksandra","family":"Jaro\u0144","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery, Pomeranian Medical University in Szczecin, 72 Powsta\u0144c\u00f3w Wlkp., 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Schneider","sequence":"additional","affiliation":[{"name":"UofL Health-Brown Cancer Center and Division of Medical Oncology and Hematology, Department of Medicine, University of Louisville, Louisville, KY 40202, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6933-7643","authenticated-orcid":false,"given":"Agnieszka","family":"Kolasa","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Pomeranian Medical University in Szczecin, 72 Powsta\u0144c\u00f3w Wlkp., 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Wiszniewska","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Pomeranian Medical University in Szczecin, 72 Powsta\u0144c\u00f3w Wlkp., 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2645465","DOI":"10.1155\/2018\/2645465","article-title":"Tight junction in the intestinal epithelium: Its association with diseases and regulation by phytochemicals","volume":"2018","author":"Lee","year":"2018","journal-title":"J. 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