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The purpose of this study is to investigate the anti-aging effect and mechanism of LRA through a D-galactose (DG)-induced aging rat model. Our results showed that the long-term intake of LRA, for 8 weeks, improved motor function, reduced serum aging markers, promoted the endogenous antioxidant system, and suppressed the serum inflammatory cytokines in aging rats. Besides, the LRA treatment alleviated DG-induced liver injuries by relieving the inflammation and inhibiting Fas\/FasL-mediated cell death. More importantly, the abnormal serum metabolome profiles of the aging rats were restored by the LRA, relating to 38 metabolites and 44 pathways. Specifically, the LRA significantly affected the amino acid and protein-related metabolic pathways by regulating the levels of L-threonine, L-aspartic acid, glycine, L-histidine, D-homocysteine, L-homocitrulline, L-homoserine, guanidineacetic acid, and kynurenine. These results have important implications for the development of LRA as an anti-aging and liver-protective ingredient.<\/jats:p>","DOI":"10.3390\/foods11213377","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T09:59:37Z","timestamp":1666778377000},"page":"3377","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Long-Term Dietary Lycium ruthenicum Murr. Anthocyanins Intake Alleviated Oxidative Stress-Mediated Aging-Related Liver Injury and Abnormal Amino Acid Metabolism"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7952-6982","authenticated-orcid":false,"given":"Shasha","family":"Chen","sequence":"first","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Tibetan Medicine Research and CAS Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Xining 810008, China"},{"name":"School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi\u2019an 710021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9882-9604","authenticated-orcid":false,"given":"Honglun","family":"Wang","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Tibetan Medicine Research and CAS Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Xining 810008, China"},{"name":"Huzhou China-Science Innovation Centre of Plateau Biology, Huzhou 313000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Hu","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Tibetan Medicine Research and CAS Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Xining 810008, China"},{"name":"Huzhou China-Science Innovation Centre of Plateau Biology, Huzhou 313000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1038\/s41586-019-1365-2","article-title":"From discoveries in ageing research to therapeutics for healthy ageing","volume":"571","author":"Campisi","year":"2019","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3085756","DOI":"10.1155\/2019\/3085756","article-title":"Oxidative Stress and Advanced Lipoxidation and Glycation End Products (ALEs and AGEs) in Aging and Age-Related Diseases","volume":"2019","author":"Moldogazieva","year":"2019","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3109\/10715762.2013.806798","article-title":"Molecular effects of advanced glycation end products on cell signalling pathways, ageing and pathophysiology","volume":"47","author":"Nedic","year":"2013","journal-title":"Free Radic. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"165612","DOI":"10.1016\/j.bbadis.2019.165612","article-title":"Current nutritional and pharmacological anti-aging interventions","volume":"1866","author":"Ros","year":"2020","journal-title":"Biochim. Biophys. Acta Mol. Basis Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1016\/j.foodchem.2017.08.026","article-title":"Lycium ruthenicum studies: Molecular biology, phytochemistry and pharmacology","volume":"240","author":"Wang","year":"2018","journal-title":"Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.foodchem.2017.08.105","article-title":"Simultaneous optimization of the ultrasound-assisted extraction for phenolic compounds content and antioxidant activity of Lycium ruthenicum Murr. fruit using response surface methodology","volume":"242","author":"Chen","year":"2018","journal-title":"Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.jff.2017.01.015","article-title":"Isolation, antioxidant property and protective effect on PC12 cell of the main anthocyanin in fruit of Lycium ruthenicum Murray","volume":"30","author":"Tang","year":"2017","journal-title":"J. Funct. 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Ameliorated d-Galactose-Induced Memory Impairment, Oxidative Stress, and Neuroinflammation in Adult Rats","volume":"67","author":"Chen","year":"2019","journal-title":"J. Agric. Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"105104","DOI":"10.1016\/j.jff.2022.105104","article-title":"The major anthocyanin of Lycium ruthenicum Murr. relieves cognitive deficits, oxidative stress, neuroinflammation, and hippocampal metabolome alterations in aging rats","volume":"94","author":"Chen","year":"2022","journal-title":"J. Funct. Foods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101164","DOI":"10.1016\/j.arr.2020.101164","article-title":"Promising biomarkers of human aging: In search of a multi-omics panel to understand the aging process from a multidimensional perspective","volume":"64","year":"2020","journal-title":"Ageing Res. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1038\/s41580-019-0108-4","article-title":"Identification of bioactive metabolites using activity metabolomics","volume":"20","author":"Rinschen","year":"2019","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e1800407","DOI":"10.1002\/pmic.201800407","article-title":"The Aging Metabolome-Biomarkers to Hub Metabolites","volume":"20","author":"Sharma","year":"2020","journal-title":"Proteomics"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e13548","DOI":"10.1111\/acel.13548","article-title":"The metabolome as a biomarker of aging in Drosophila melanogaster","volume":"21","author":"Zhao","year":"2022","journal-title":"Aging Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1093\/gerona\/glv074","article-title":"Human Aging Is a Metabolome-related Matter of Gender","volume":"71","author":"Pamplona","year":"2016","journal-title":"J. Gerontol. Ser. A Biol. Sci. Med. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1007\/s11357-019-00123-w","article-title":"The plasma metabolome as a predictor of biological aging in humans","volume":"41","author":"Johnson","year":"2019","journal-title":"GeroScience"},{"key":"ref_23","first-page":"536","article-title":"Untargeted Metabolomic Profiling of Liver and Serum in Mouse during Normal Aging","volume":"43","author":"Liu","year":"2021","journal-title":"Zhongguo Yi Xue Ke Xue Yuan Xue Bao Acta Acad. Med. Sin."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"830723","DOI":"10.3389\/fmed.2021.830723","article-title":"Metabolomics-Based Frailty Biomarkers in Older Chinese Adults","volume":"8","author":"Pan","year":"2021","journal-title":"Front. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s10522-019-09837-y","article-title":"D-Galactose-induced accelerated aging model: An overview","volume":"20","author":"Azman","year":"2019","journal-title":"Biogerontology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e17","DOI":"10.1093\/labmed\/lmaa059","article-title":"The Utility of Elevated Serum Lactate Dehydrogenase in Current Clinical Practice","volume":"52","author":"Krishnamurthy","year":"2021","journal-title":"Lab. Med."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Rupasinghe, H.P.V., and Arumuggam, N. (2019). Chapter 5. Health benefits of anthocyanins. Anthocyanins from Natural Sources: Exploiting Targeted Delivery for Improved Health, The Royal Society of Chemistry.","DOI":"10.1039\/9781788012614-00121"},{"key":"ref_28","first-page":"153509","article-title":"Anti-aging function and molecular mechanism of Radix Astragali and Radix Astragali preparata via network pharmacology and PI3K\/Akt signaling pathway","volume":"84","author":"Gong","year":"2021","journal-title":"Phytomed. Int. J. Phytother. Phytopharm."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/j.nut.2016.01.007","article-title":"Dietary sweet cherry anthocyanins attenuates diet-induced hepatic steatosis by improving hepatic lipid metabolism in mice","volume":"32","author":"Song","year":"2016","journal-title":"Nutrition"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"109964","DOI":"10.1016\/j.foodres.2020.109964","article-title":"Beneficial effects of anthocyanin-rich peels of Myrtaceae fruits on chemically-induced liver fibrosis and carcinogenesis in mice","volume":"139","author":"Romualdo","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"101843","DOI":"10.1016\/j.clinre.2021.101843","article-title":"Upregulated NLRP3 inflammasome activation is attenuated by anthocyanins in patients with nonalcoholic fatty liver disease: A case-control and an intervention study","volume":"46","author":"Zhu","year":"2022","journal-title":"Clin. Res. Hepatol. Gastroenterol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"110922","DOI":"10.1016\/j.fct.2019.110922","article-title":"Impact of dietary anthocyanins on systemic and vascular inflammation: Systematic review and meta-analysis on randomised clinical trials","volume":"135","author":"Fallah","year":"2020","journal-title":"Food Chem. Toxicol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Srivastava, S. (2019). Emerging Insights into the Metabolic Alterations in Aging Using Metabolomics. Metabolites, 9.","DOI":"10.3390\/metabo9120301"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1016\/j.cell.2012.02.032","article-title":"Cellular metabolism and disease: What do metabolic outliers teach us?","volume":"148","author":"DeBerardinis","year":"2012","journal-title":"Cell"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4103","DOI":"10.1039\/D0FO00633E","article-title":"Astaxanthin attenuates d-galactose-induced brain aging in rats by ameliorating oxidative stress, mitochondrial dysfunction, and regulating metabolic markers","volume":"11","author":"Liu","year":"2020","journal-title":"Food Funct."},{"key":"ref_36","first-page":"e14597","article-title":"Sex differences in body composition, metabolism-related hormones, and energy homeostasis during aging in Wistar rats","volume":"8","author":"Reis","year":"2020","journal-title":"Physiol. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2532s","DOI":"10.1093\/jn\/nxaa118","article-title":"Alterations in Sulfur Amino Acids as Biomarkers of Disease","volume":"150","author":"Stabler","year":"2020","journal-title":"J. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"101179","DOI":"10.1016\/j.jbc.2021.101179","article-title":"Dietary lysine restriction induces lipid accumulation in skeletal muscle through an increase in serum threonine levels in rats","volume":"297","author":"Goda","year":"2021","journal-title":"J. Biol. Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.cmet.2020.04.018","article-title":"Untangling Determinants of Enhanced Health and Lifespan through a Multi-omics Approach in Mice","volume":"32","author":"Aon","year":"2020","journal-title":"Cell Metab."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"C818","DOI":"10.1152\/ajpcell.00580.2019","article-title":"The kynurenine connection: How exercise shifts muscle tryptophan metabolism and affects energy homeostasis, the immune system, and the brain","volume":"318","author":"Martin","year":"2020","journal-title":"Am. J. Physiol. Cell Physiol."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/21\/3377\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:03:29Z","timestamp":1760144609000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/21\/3377"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,26]]},"references-count":40,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["foods11213377"],"URL":"https:\/\/doi.org\/10.3390\/foods11213377","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,26]]}}}