{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T19:30:12Z","timestamp":1792006212263,"version":"4.4.0"},"reference-count":81,"publisher":"MDPI AG","issue":"22","license":[{"start":{"date-parts":[[2022,11,8]],"date-time":"2022-11-08T00:00:00Z","timestamp":1667865600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology","award":["LR16IPT03"],"award-info":[{"award-number":["LR16IPT03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Globally, Campylobacter is a significant contributor to gastroenteritis. Efficient pathogens are qualified by their virulence power, resistance to antibiotics and epidemic spread. However, the correlation between antimicrobial resistance (AR) and the pathogenicity power of pathogens is complex and poorly understood. In this study, we aimed to investigate genes encoding virulence and AR mechanisms in 177 Campylobacter isolates collected from layer hens and eggs in Tunisia and to assess associations between AR and virulence characteristics. Virulotyping was determined by searching 13 virulence genes and AR-encoding genes were investigated by PCR and MAMA-PCR. The following genes were detected in C. jejuni and C. coli isolates: tet(O) (100%\/100%), blaOXA-61 (18.82%\/6.25%), and cmeB (100%\/100%). All quinolone-resistant isolates harbored the Thr-86-Ile substitution in GyrA. Both the A2074C and A2075G mutations in 23S rRNA were found in all erythromycin-resistant isolates; however, the erm(B) gene was detected in 48.38% and 64.15% of the C. jejuni and C. coli isolates, respectively. The machine learning algorithm Random Forest was used to determine the association of virulence genes with AR phenotypes. This analysis showed that C. jejuni virulotypes with gene clusters encompassing the racR, ceuE, virB11, and pldA genes were strongly associated with the majority of phenotypic resistance. Our findings showed high rates of AR and virulence genes among poultry Campylobacter, which is a cause of concern to human health. In addition, the correlations of specific virulence genes with AR phenotypes were established by statistical analysis.<\/jats:p>","DOI":"10.3390\/foods11223554","type":"journal-article","created":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T02:34:52Z","timestamp":1667961292000},"page":"3554","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Relationships between Virulence Genes and Antibiotic Resistance Phenotypes\/Genotypes in Campylobacter spp. Isolated from Layer Hens and Eggs in the North of Tunisia: Statistical and Computational Insights"],"prefix":"10.3390","volume":"11","author":[{"given":"Manel","family":"Gharbi","sequence":"first","affiliation":[{"name":"Group of Bacteriology and Biotechnology Development, Laboratory of Epidemiology and Veterinary Microbiology, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selim","family":"Kamoun","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics, Biomathematics and Biostatistics, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1006, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9167-2480","authenticated-orcid":false,"given":"Chaima","family":"Hkimi","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics, Biomathematics and Biostatistics, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1006, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3387-6493","authenticated-orcid":false,"given":"Kais","family":"Ghedira","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics, Biomathematics and Biostatistics, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1006, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Awatef","family":"B\u00e9jaoui","sequence":"additional","affiliation":[{"name":"Group of Bacteriology and Biotechnology Development, Laboratory of Epidemiology and Veterinary Microbiology, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abderrazak","family":"Maaroufi","sequence":"additional","affiliation":[{"name":"Group of Bacteriology and Biotechnology Development, Laboratory of Epidemiology and Veterinary Microbiology, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"108656","DOI":"10.1016\/j.ijfoodmicro.2020.108656","article-title":"Prevalence, antibiotic Rrsistance and genotypes of Campylobacter Jejuni and Campylobacter coli isolated from chickens in Irbid governorate, Jordan","volume":"327","author":"Alaboudi","year":"2020","journal-title":"Int. J. Food Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.mimet.2015.10.018","article-title":"EUCAST Recommendations for Antimicrobial Susceptibility Testing Applied to the Three Main Campylobacter Species Isolated in Humans","volume":"119","author":"Salha","year":"2015","journal-title":"J. Microbiol. Methods"},{"key":"ref_3","first-page":"e06406","article-title":"The European Union One Health 2019 zoonoses report","volume":"19","author":"Food","year":"2021","journal-title":"EFSA J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e02814","DOI":"10.1016\/j.heliyon.2019.e02814","article-title":"Human Campylobacteriosis: A public health concern of global Importance","volume":"5","author":"Igwaran","year":"2019","journal-title":"Heliyon"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2020.592496","article-title":"Antibiotic Resistance Profiles and Molecular Mechanisms of Campylobacter from Chicken and Pig in China","volume":"11","author":"Tang","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1093\/jac\/dkv382","article-title":"Species Shift and Multidrug Resistance of Campylobacter from Chicken and Swine, China, 2008\u20132014","volume":"71","author":"Wang","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1351","DOI":"10.1021\/cb300105p","article-title":"Visualizing the 16-membered ring macrolides tildipirosin and tilmicosin bound to their ribosomal site","volume":"7","author":"Poehlsgaard","year":"2012","journal-title":"ACS Chem. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"6-2","DOI":"10.1128\/microbiolspec.ARBA-0009-2017","article-title":"Antimicrobial resistance: A One Health perspective","volume":"6","author":"McEwen","year":"2018","journal-title":"Microbiol. Spectr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1128\/CMR.00006-15","article-title":"Global epidemiology of campylobacter infection","volume":"28","author":"Kaakoush","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"311","DOI":"10.7883\/yoken.JJID.2016.309","article-title":"High rates of contamination of poultry meat products with drug-resistant campylobacter in Metro Manila, Philippines","volume":"70","author":"Lim","year":"2017","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"516","DOI":"10.3389\/fvets.2020.00516","article-title":"Challenges to the poultry industry: Current perspectives and strategic future after the COVID-19 outbreak","volume":"7","author":"Hafez","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1128\/MMBR.65.2.232-260.2001","article-title":"Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance","volume":"65","author":"Chopra","year":"2001","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2017.02254","article-title":"Prevalence and Antibiotic Resistance against Tetracycline in Campylobacter Jejuni and C. Coli in Cattle and Beef Meat from Selangor, Malaysia","volume":"8","author":"Premarathne","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1089\/mdr.2016.0249","article-title":"High Prevalence of Resistance to Fluoroquinolones and Tetracycline Campylobacter spp. Isolated from Poultry in Poland","volume":"24","author":"Kielsznia","year":"2018","journal-title":"Microb. Drug Resist."},{"key":"ref_15","first-page":"1","article-title":"Human Campylobacteriosis in Italy: Emergence of Multi-Drug Resistance to Ciprofloxacin, Tetracycline, and Erythromycin","volume":"9","author":"Dionisi","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"217","DOI":"10.7883\/yoken.JJID.2007.217","article-title":"Isolation and Drug-Resistant Patterns of Campylobacter Strains Cultured from Diarrheic Children in Tehran","volume":"60","author":"Feizabadi","year":"2007","journal-title":"Jpn J. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1089\/fpd.2016.2186","article-title":"Antimicrobial Resistance of Campylobacter Species Isolated from Broilers in Live Bird Markets in Shanghai, China","volume":"14","author":"Li","year":"2017","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12879-020-05006-6","article-title":"Risk of Multi-Drug Resistant Campylobacter spp. And Residual Antimicrobials at Poultry Farms and Live Bird Markets in Bangladesh","volume":"20","author":"Neogi","year":"2020","journal-title":"BMC Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"230","DOI":"10.4161\/viru.23753","article-title":"Resistance mechanisms in Campylobacter jejuni","volume":"4","author":"Iovine","year":"2013","journal-title":"Virulence"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.femsle.2005.02.034","article-title":"Update on Acquired Tetracycline Resistance Genes","volume":"245","author":"Roberts","year":"2005","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Roberts, M.C. (2007). Genetic mobility and distribution of tetracycline resistance determinants. Ciba Foundation Symposium 207-Antibiotic Resistance: Origins, Evolution, Selection and Spread: Antibiotic Resistance: Origins, Evolution, Selection and Spread: Ciba Foundation Symposium 207, John Wiley & Sons, Ltd.","DOI":"10.1002\/9780470515358.ch13"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Horme\u00f1o, L., Campos, M.J., Vadillo, S., and Quesada, A. (2020). Occurrence of tet(O\/M\/O) mosaic gene in tetracycline-resistant Campylobacter. Microorganisms, 8.","DOI":"10.3390\/microorganisms8111710"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s13099-019-0338-1","article-title":"Investigation of Molecular Mechanisms Underlying Tetracycline Resistance in Thermophilic Campylobacter spp. Suggests That Previous Reports of Tet(A)-Mediated Resistance in These Bacteria Are Premature","volume":"11","author":"Lynch","year":"2019","journal-title":"Gut Pathog."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fvets.2020.00227","article-title":"Characterization and Prevalence of Campylobacter Spp. From Broiler Chicken Rearing Period to the Slaughtering Process in Eastern China","volume":"7","author":"Tang","year":"2020","journal-title":"Front. Vet. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3442","DOI":"10.1128\/AAC.48.9.3442-3450.2004","article-title":"Incidence of antibiotic resistance in Campylobacter jejuni isolated in Alberta, Canada, from 1999 to 2002, with special reference to tet(O)-mediated tetracycline resistance","volume":"48","author":"Gibreel","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"189","DOI":"10.2217\/17460913.4.2.189","article-title":"Antibiotic Resistance in Campylobacter: Emergence, Transmission and Persistence","volume":"4","author":"Luangtongkum","year":"2009","journal-title":"Future Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2124","DOI":"10.1128\/AAC.46.7.2124-2131.2002","article-title":"CmeABC Functions as a Multidrug Efflux System in Campylobacter jejuni","volume":"46","author":"Lin","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1093\/jac\/dkt492","article-title":"Report of Ribosomal RNA Methylase Gene Erm(B) in Multidrug-Resistant Campylobacter coli","volume":"69","author":"Qin","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1128\/CMR.00059-12","article-title":"Antimicrobial Resistance and Virulence: A Successful or Deleterious Association in the Bacterial World?","volume":"26","author":"Beceiro","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jhin.2013.07.006","article-title":"Contaminated Sinks in Intensive Care Units: An Underestimated Source of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae in the Patient Environment","volume":"85","author":"Roux","year":"2013","journal-title":"J. Hosp. Infect."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.4315\/0362-028X-72.5.1121","article-title":"Stress, Sublethal Injury, Resuscitation, and Virulence of Bacterial Foodborne Pathogens","volume":"72","author":"Wesche","year":"2009","journal-title":"J. Food Prot."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1126\/science.1172914","article-title":"The SOS Response Controls Integron Recombination","volume":"324","author":"Guerin","year":"2009","journal-title":"Science"},{"key":"ref_33","unstructured":"(2006). Microbiology of Food and Animal Feeding Stuffs: Horizontal Method for Detection and Enumera-tion of Campylobacter spp. I. Detection Method (Standard No. ISO 10272-1 (Annex E))."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Gharbi, M., B\u00e9jaoui, A., Ben Hamda, C., Alaya, N., Hamrouni, S., Bessoussa, G., Ghram, A., and Maaroufi, A. (2022). Campylobacter spp. in Eggs and Laying Hens in the North-East of Tunisia: High Prevalence and Multidrug-Resistance Phenotypes. Vet. Sci., 9.","DOI":"10.3390\/vetsci9030108"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"7943786","DOI":"10.1155\/2018\/7943786","article-title":"Prevalence and Antibiotic Resistance Patterns of Campylobacter spp. Isolated from Broiler Chickens in the North of Tunisia","volume":"2018","author":"Gharbi","year":"2018","journal-title":"BioMed Res. Int."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Gharbi, M., B\u00e9jaoui, A., Ben Hamda, C., Ghedira, K., Ghram, A., and Maaroufi, A. (2021). Distribution of Virulence and Antibiotic Resistance Genes in Campylobacter jejuni and Campylobacter coli Isolated from Broiler Chickens in Tunisia. J. Microbiol. Immunol. Infect.","DOI":"10.1016\/j.jmii.2021.07.001"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2000.tb09253.x","article-title":"Characterization of GyrA Mutations Associated with Fluoroquinolone Resistance in Campylobacter coli by DNA Sequence Analysis and MAMA PCR","volume":"190","author":"Zirnstein","year":"2000","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3276","DOI":"10.1128\/JCM.37.10.3276-3280.1999","article-title":"Ciprofloxacin Resistance in Campylobacter jejuni Isolates: Detection of GyrA Resistance Mutations by Mismatch Amplification Mutation Assay PCR and DNA Sequence Analysis","volume":"37","author":"Zirnstein","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.mimet.2005.03.013","article-title":"MAMA-PCR Assay for the Detection of Point Mutations Associated with High-Level Erythromycin Resistance in Campylobacter jejuni and Campylobacter coli Strains","volume":"63","author":"Alonso","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"ref_40","first-page":"18","article-title":"Classification and Regression by RandomForest","volume":"2","author":"Liaw","year":"2002","journal-title":"R News"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Martinez-Taboada, F., and Redondo, J.I. (2020). The SIESTA (SEAAV Integrated Evaluation Sedation Tool for Anaesthesia) Project: Initial Development of a Multifactorial Sedation Assessment Tool for Dogs. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0230799"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1093\/jac\/dkh505","article-title":"Reduction of the Fitness Burden of Quinolone Resistance in Pseudomonas Aeruginosa","volume":"55","author":"Kugelberg","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"20747","DOI":"10.1073\/pnas.1221552110","article-title":"Enhanced in Vivo Fitness of Carbapenem-Resistant OprD Mutants of Pseudomonas Aeruginosa Revealed through High-Throughput Sequencing","volume":"110","author":"Skurnik","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2541","DOI":"10.1021\/acs.est.0c05274","article-title":"Degradation Kinetics of Antibiotic Resistance Gene Meca of Methicillin-Resistant Staphylococcus Aureus (Mrsa) during Water Disinfection with Chlorine, Ozone, and Ultraviolet Light","volume":"55","author":"Lee","year":"2021","journal-title":"Environ. Sci. Technol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.3389\/fmicb.2018.01014","article-title":"Genetic basis and clonal population structure of antibiotic resistance in Campylobacter jejuni isolated from broiler carcasses in Belgium","volume":"9","author":"Elhadidy","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Lynch, C.T., Lynch, H., Burke, S., Hawkins, K., Buttimer, C., Mc Carthy, C., Egan, J., Whyte, P., Bolton, D., and Coffey, A. (2020). Antimicrobial Resistance Determinants Circulating among Thermophilic Campylobacter Isolates Recovered from Broilers in Ireland Over a One-Year Period. Antibiotics, 9.","DOI":"10.3390\/antibiotics9060308"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Song, D.G., Yoon, K.Y., Mboera, L.E.G., Matee, M.I., Mutangana, D., Komba, E.V.G., Pan, C.H., and Amachawadi, R.G. (2021). Genomic Characterization of Fluoroquinolone-Resistant Thermophilic Campylobacter Strains Isolated from Layer Chicken Feces in Gangneung, South Korea by Whole-Genome Sequencing. Genes, 12.","DOI":"10.3390\/genes12081131"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.3389\/fmicb.2019.02384","article-title":"Successful Host Adaptation of IncK2 Plasmids","volume":"10","author":"Rozwandowicz","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.3382\/ps.2013-03729","article-title":"Antimicrobial Resistance among Campylobacter spp. Strains Isolated from Different Poultry Production Systems at Slaughterhouse Level","volume":"93","author":"Fraqueza","year":"2014","journal-title":"Poult. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1089\/mdr.2020.0184","article-title":"Antimicrobial Resistance Profiles and Phylogenetic Analysis of Campylobacter jejuni Strains Isolated in Brazil by Whole Genome Sequencing","volume":"27","author":"Cao","year":"2021","journal-title":"Microb. Drug Resist."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Kim, J., Park, H., Kim, J., Kim, J.H., Jung, J.I., Cho, S., Ryu, S., and Jeon, B. (2019). Comparative Analysis of Aerotolerance, Antibiotic Resistance, and Virulence Gene Prevalence in Campylobacter jejuni Isolates from Retail Raw Chicken and Duck Meat in South Korea. Microorganisms, 7.","DOI":"10.3390\/microorganisms7100433"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e03537","DOI":"10.1016\/j.heliyon.2020.e03537","article-title":"Epidemiology and Antimicrobial Resistance of Campylobacter spp. in Animals in Sub-Saharan Africa: A Systematic Review","volume":"6","author":"Hlashwayo","year":"2020","journal-title":"Heliyon"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1089\/fpd.2018.2477","article-title":"Lack of Evidence for Erm (B) Infiltration into Erythromycin-Resistant Campylobacter coli and Campylobacter jejuni from Commercial Turkey Production in Eastern North Carolina: A Major Turkey-Growing Region in the United States","volume":"15","author":"Bolinger","year":"2018","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e02615-17","DOI":"10.1128\/AAC.02615-17","article-title":"Report of Erm (B)+ Campylobacter jejuni in the United States. Antimicrob","volume":"62","author":"Chen","year":"2018","journal-title":"Agents Chemother."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1089\/mdr.2015.0079","article-title":"Prevalence and Antimicrobial Resistance of Campylobacter Isolated from Dressed Beef Carcasses and Raw Milk in Tanzania","volume":"22","author":"Kashoma","year":"2016","journal-title":"Microb. drug Resist."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"De Vries, S.P.W., Vurayai, M., Holmes, M., Gupta, S., Bateman, M., Goldfarb, D., Maskell, D.J., Matsheka, M.I., and Grant, A.J. (2018). Phylogenetic Analyses and Antimicrobial Resistance Profiles of Campylobacter spp. from Diarrhoeal Patients and Chickens in Botswana. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0194481"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/zph.12208","article-title":"Antimicrobial Resistance Profiles of Campylobacter spp. Isolated from Broiler Chicken Meat of Estonian, Latvian and Lithuanian Origin at Estonian Retail Level and from Patients with Severe Enteric Infections in Estonia","volume":"63","author":"Kramarenko","year":"2016","journal-title":"Zoonoses Public Health"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1089\/fpd.2017.2325","article-title":"Characterization of Campylobacter jejuni and Campylobacter coli Broiler Isolates by Whole-Genome Sequencing","volume":"15","author":"Cantero","year":"2018","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Pilay, S., Amoako, D.G., Abia, A.L.K., Somboro, A.M., Shobo, C.O., Perrett, K., Bester, L.A., and Essack, S.Y. (2020). Characterisation of Campylobacter spp. Isolated from Poultry in KwaZulu-Natal, South Africa. Antibiotics, 9.","DOI":"10.3390\/antibiotics9020042"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Elhadidy, M., Ali, M.M., El-Shibiny, A., Miller, W.G., Elkhatib, W.F., Botteldoorn, N., and Dierick, K. (2021). Antimicrobial resistance patterns and molecular resistance markers of Campylobacter jejuni isolates from human diarrheal cases. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0227833"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.foodcont.2019.04.038","article-title":"Prevalence, Antimicrobial Resistance Profiles and Virulence-Associated Genes of Thermophilic Campylobacter spp. Isolated from Ducks in a Chinese Slaughterhouse","volume":"104","author":"Han","year":"2019","journal-title":"Food Control."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s12275-017-6308-8","article-title":"Epidemiological Relationships of Campylobacter Jejuni Strains Isolated from Humans and Chickens in South Korea","volume":"55","author":"Oh","year":"2017","journal-title":"J. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.micpath.2017.12.057","article-title":"Detection of Virulence Genes Determining the Ability to Adhere and Invade in Campylobacter spp. from Cattle and Swine in Poland","volume":"115","author":"Wysok","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_64","first-page":"E79","article-title":"Campylobacter: From Microbiology to Prevention","volume":"58","author":"Riso","year":"2017","journal-title":"J. Prev. Med. Hyg."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Otigbu, A.C., Clarke, A.M., Fri, J., Akanbi, E.O., and Njom, H.A. (2018). Antibiotic Sensitivity Profiling and Virulence Potential of Campylobacter jejuni Isolates from Estuarine Water in the Eastern Cape Province, South Africa. Int. J. Environ. Res. Public Health, 15.","DOI":"10.3390\/ijerph15050925"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1080\/19476337.2012.722564","article-title":"Campylobacter jejuni Isolated from Human Cases in Chile Showed Indistinguishable Pulsed Field Gel Electrophoresis Profiles with Strains Isolated from Poultry and Bovine Sources","volume":"11","author":"Foerster","year":"2013","journal-title":"CyTA-J. Food"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1007\/s42770-020-00314-0","article-title":"Genetic Diversity, Antimicrobial Resistance, and Virulence Genes of Thermophilic Campylobacter Isolated from Broiler Production Chain","volume":"51","author":"Ramires","year":"2020","journal-title":"Braz. J. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1292\/jvms.71.1247","article-title":"Age-Dependent Variation of Virulence-Associated Genes Retained in Campylobacter jejuni Isolated from Chickens in a Poultry Farm","volume":"71","author":"Datta","year":"2009","journal-title":"J. Vet. Med. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1089\/fpd.2013.1489","article-title":"Distribution of Nine Virulence-Associated Genes in Campylobacter Jejuni and C. Coli Isolated from Broiler Feces in Shiraz, Southern Iran","volume":"10","author":"Khoshbakht","year":"2013","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"3896","DOI":"10.1074\/jbc.275.6.3896","article-title":"Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384: Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-MHz 1H and 13C NMR analysis","volume":"275","author":"Gilbert","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1046\/j.1365-2958.2000.02020.x","article-title":"Phase variation of a \u03b2-1, 3 galactosyltransferase involved in generation of the ganglioside GM1-like lipo-oligosaccharide of Campylobacter jejuni","volume":"37","author":"Linton","year":"2000","journal-title":"Mol. Microbiol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1099\/jmm.0.05056-0","article-title":"Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces","volume":"52","author":"Datta","year":"2003","journal-title":"J. Med. Microbiol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1128\/JCM.37.3.510-517.1999","article-title":"Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product","volume":"37","author":"Konkel","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/1297-9716-42-82","article-title":"Colonization factors of Campylobacter jejuni in the chicken gut","volume":"42","author":"Hermans","year":"2011","journal-title":"Vet. Res."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"549","DOI":"10.2307\/1592894","article-title":"Role of Campylobacter jejuni potential virulence genes in cecal colonization","volume":"45","author":"Ziprin","year":"2001","journal-title":"Avian Dis."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"6535","DOI":"10.1128\/IAI.68.12.6535-6541.2000","article-title":"Campylobacter jejuni cytolethal distending toxin mediates release of interleukin- from intestinal epithelial cells","volume":"68","author":"Hickey","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/S0923-2508(97)85118-2","article-title":"Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals","volume":"147","author":"Linton","year":"1996","journal-title":"Res. Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1128\/jcm.35.3.759-763.1997","article-title":"Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant","volume":"35","author":"Gonzalez","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1093\/jac\/dki040","article-title":"Tetracycline resistance of Australian Campylobacter jejuni and Campylobacter coli isolates","volume":"55","author":"Pratt","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.fm.2005.06.004","article-title":"Prevalence of the Campylobacter multi-drug efflux pump (CmeABC) in Campylobacter spp. Isolated from freshly processed turkeys","volume":"23","author":"Olah","year":"2006","journal-title":"Food Microbiol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1111\/j.1365-2672.2012.05354.x","article-title":"Antimicrobial susceptibilities and resistance genes in Campylobacter strains isolated from poultry and pigs in Australia","volume":"113","author":"Obeng","year":"2012","journal-title":"J. Appl. Microbiol."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/22\/3554\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:12:42Z","timestamp":1760145162000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/22\/3554"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,8]]},"references-count":81,"journal-issue":{"issue":"22","published-online":{"date-parts":[[2022,11]]}},"alternative-id":["foods11223554"],"URL":"https:\/\/doi.org\/10.3390\/foods11223554","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,8]]}}}