{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:26:01Z","timestamp":1792621561836,"version":"4.5.0"},"reference-count":76,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:00:00Z","timestamp":1671062400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Shanghai Municipal Science and Technology Commission","doi-asserted-by":"publisher","award":["17050502200"],"award-info":[{"award-number":["17050502200"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Shanghai Municipal Science and Technology Commission","doi-asserted-by":"publisher","award":["31671946"],"award-info":[{"award-number":["31671946"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["17050502200"],"award-info":[{"award-number":["17050502200"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31671946"],"award-info":[{"award-number":["31671946"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Klebsiella pneumoniae can cause serious pneumonitis in humans. The bacterium is also the common causative agent of hospital-acquired multidrug-resistant (MDR) infections. Here we for the first time reported the genetic diversity of K. pneumoniae strains in 14 species of edible aquatic animals sampled in the summer of 2018 and 2019 in Shanghai, China. Virulence-related genes were present in the K. pneumoniae strains (n = 94), including the entB (98.9%), mrkD (85.1%), fimH (50.0%), and ybtA (14.9%) strains. Resistance to sulfamethoxazole-trimethoprim was the most prevalent (52.1%), followed by chloramphenicol (31.9%), and tetracycline (27.7%), among the strains, wherein 34.0% had MDR phenotypes. Meanwhile, most strains were tolerant to heavy metals Cu2+ (96.8%), Cr3+ (96.8%), Zn2+ (91.5%), Pb2+ (89.4%), and Hg2+ (81.9%). Remarkably, a higher abundance of the bacterium was found in bottom-dwelling aquatic animals, among which mollusk Tegillarca granosa contained K. pneumoniae 8-2-5-4 isolate from serotype K2 (ST-2026). Genome features of the potentially pathogenic isolate were characterized. The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR)\u2013based genome fingerprinting classified the 94 K. pneumoniae strains into 76 ERIC genotypes with 63 singletons, demonstrating considerable genetic diversity in the strains. The findings of this study fill the gap in the risk assessment of K. pneumoniae in edible aquatic animals.<\/jats:p>","DOI":"10.3390\/foods11244058","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T02:16:41Z","timestamp":1671157001000},"page":"4058","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["First Report of Potentially Pathogenic Klebsiella pneumoniae from Serotype K2 in Mollusk Tegillarca granosa and Genetic Diversity of Klebsiella pneumoniae in 14 Species of Edible Aquatic Animals"],"prefix":"10.3390","volume":"11","author":[{"given":"Yingwei","family":"Xu","sequence":"first","affiliation":[{"name":"Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Ni","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqiong","family":"Guan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dailing","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0788-2099","authenticated-orcid":false,"given":"Si","family":"Qin","sequence":"additional","affiliation":[{"name":"Lab of Food Function and Nutrigenomics, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanming","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People\u2019s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1128\/CMR.11.4.589","article-title":"Klebsiella spp. as nosocomial pathogens: Epidemiology, taxonomy, typing methods, and pathogenicity factors","volume":"11","author":"Podschun","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"E3574","DOI":"10.1073\/pnas.1501049112","article-title":"Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health","volume":"112","author":"Holt","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1097\/MD.0000000000000111","article-title":"Klebsiella pneumoniae bloodstream infection: Epidemiology and impact of inappropriate empirical therapy","volume":"93","author":"Girometti","year":"2014","journal-title":"Medicine"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1128\/MMBR.00078-15","article-title":"Klebsiella pneumoniae: Going on the offense with a strong defense","volume":"80","author":"Paczosa","year":"2016","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/BF01880516","article-title":"Ueber die Schizomyceten bei der acuten fibr\u00f6sen Pneumonie","volume":"87","author":"Friedlaender","year":"1882","journal-title":"Archiv. Pathol. Anat."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Wang, G., Zhao, G., Chao, X., Xie, L., and Wang, H. (2020). The characteristic of virulence, biofilm and antibiotic resistance of Klebsiella pneumoniae. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17176278"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.2217\/fmb.14.48","article-title":"Molecular pathogenesis of Klebsiella pneumoniae","volume":"9","author":"Li","year":"2014","journal-title":"Future Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.jgar.2021.02.020","article-title":"A global perspective on the convergence of hypervirulence and carbapenem resistance in Klebsiella pneumoniae","volume":"25","author":"Lan","year":"2021","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1099\/jmm.0.015198-0","article-title":"PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets","volume":"59","author":"Turton","year":"2010","journal-title":"J. Med. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1099\/jmm.0.46964-0","article-title":"Genetically similar isolates of Klebsiella pneumoniae serotype K1 causing liver abscesses in three continents","volume":"56","author":"Turton","year":"2007","journal-title":"J. Med. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.jgar.2020.06.009","article-title":"Molecular mechanisms and prevalence of colistin resistance of Klebsiella pneumoniae in the middle east region: A review over the last 5 years","volume":"22","author":"Aris","year":"2020","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"38929","DOI":"10.1038\/srep38929","article-title":"Molecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitals","volume":"6","author":"Wasfi","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"165","DOI":"10.3389\/fcimb.2016.00165","article-title":"Clinical and genomic analysis of liver abscess-causing Klebsiella pne umoniae identifies new liver abscess-associated virulence genes","volume":"6","author":"Ye","year":"2016","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.micpath.2018.01.030","article-title":"Frequency of virulence factors in high biofilm formation bla (KPC-2) producing Klebsiella pneumoniae strains from hospitals","volume":"116","author":"Fu","year":"2018","journal-title":"Microb. Pathog."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.diagmicrobio.2008.04.007","article-title":"Comparison of prevalence of virulence factors for Klebsiella pneumoniae liver abscesses between isolates with capsular K1\/K2 and non-K1\/K2 serotypes","volume":"62","author":"Yu","year":"2008","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_16","unstructured":"European Centre for Disease Prevention and Control (ECDC) (2022). Antimicrobial Consumption in the EU\/EEA (ESAC-Net)\u2014Annual Epidemiological Report 2021."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.jgar.2020.09.004","article-title":"Virulence evolution, molecular mechanisms of resistance and prevalence of ST11 carbapenem-resistant Klebsiella pneumoniae in China: A review over the last 10 years","volume":"23","author":"Liao","year":"2020","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.jgar.2020.01.009","article-title":"Presence of heavy metal resistance genes in Escherichia coli and Salmonella isolates and analysis of resistance gene structure in E. coli E308","volume":"21","author":"Yang","year":"2020","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1128\/MMBR.00016-10","article-title":"Origins and evolution of antibiotic resistance","volume":"74","author":"Davies","year":"2010","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"106892","DOI":"10.1016\/j.envint.2021.106892","article-title":"Current and future threats to human health in the Anthropocene","volume":"158","author":"Tong","year":"2022","journal-title":"Environ. Int."},{"key":"ref_21","first-page":"402647","article-title":"Heavy metals in contaminated soils: A review of sources, chemistry, risks and best available strategies for remediation","volume":"2011","author":"Wuana","year":"2011","journal-title":"ISRN Ecol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"108225","DOI":"10.1016\/j.foodcont.2021.108225","article-title":"Residual levels of antimicrobial agents and heavy metals in 41 species of commonly consumed aquatic products in Shanghai, China, and cumulative exposure risk to children and teenagers","volume":"129","author":"Ni","year":"2021","journal-title":"Food Control"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"399","DOI":"10.3389\/fmicb.2012.00399","article-title":"Heavy metal driven co-selection of antibiotic resistance in soil and water bodies impacted by agriculture and aquaculture","volume":"3","author":"Seiler","year":"2012","journal-title":"Front. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"898125","DOI":"10.3389\/fcimb.2022.898125","article-title":"Polymyxin resistance in clinical isolates of K. pneumoniae in Brazil: Update on molecular mechanisms, clonal dissemination and relationship with KPC-producing strains","volume":"12","author":"Pontes","year":"2022","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Van Hooste, W., Vanrentergem, M., Nulens, E., Snauwaert, C., De Geyter, D., Mertens, R., and Van Praet, J.T. (2022). Infections caused by hypervirulent Klebsiella pneumoniae in non-endemic countries: Three case reports and review of the literature. Acta Clin. Belg., 1\u20135.","DOI":"10.1080\/17843286.2022.2106705"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1093\/cid\/civ433","article-title":"Editorial commentary: Genomic epidemiology: Revealing hidden reservoirs for Klebsiella pneumoniae","volume":"61","author":"Manges","year":"2015","journal-title":"Clin. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"H\u00e5konsholm, F., Hetland, M.A.K., Svanevik, C.S., Sundsfjord, A., Lunestad, B.T., and Marathe, N.P. (2020). Antibiotic sensitivity screening of Klebsiella spp. and Raoultella spp. isolated from marine bivalve molluscs reveal presence of CTX-M-producing K. pneumoniae. Microorganisms, 8.","DOI":"10.3390\/microorganisms8121909"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1128\/CMR.00002-11","article-title":"Food animals and antimicrobials: Impacts on human health","volume":"24","author":"Marshall","year":"2011","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.resmic.2018.11.003","article-title":"Role of the Klebsiella pneumoniae TolC porin in antibiotic efflux","volume":"170","author":"Iyer","year":"2019","journal-title":"Res. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"353","DOI":"10.2165\/00003495-200363040-00002","article-title":"Extended spectrum beta-lactamase (ESBL)-producing enterobacteriaceae: Considerations for diagnosis, prevention and drug treatment","volume":"63","author":"Rupp","year":"2003","journal-title":"Drugs"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.4315\/JFP-19-520","article-title":"Foodborne Klebsiella pneumoniae: Virulence potential, antibiotic resistance, and risks to food safety","volume":"83","author":"Hartantyo","year":"2020","journal-title":"J. Food Prot."},{"key":"ref_32","unstructured":"Block, S.S. (2000). Disinfection, Sterilization, and Preservation, Lippincott Williams & Wilkins. [5th ed.]."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Chen, D., Li, X., Ni, L., Xu, D., Xu, Y., Ding, Y., Xie, L., and Chen, L. (2021). First experimental evidence for the presence of potentially toxic Vibrio cholerae in snails, and virulence, cross-resistance and genetic diversity of the bacterium in 36 species of aquatic food animals. Antibiotics, 10.","DOI":"10.3390\/antibiotics10040412"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Ni, L., Xu, Y., and Chen, L. (2021). First experimental evidence for the presence of potentially virulent Klebsiella oxytoca in 14 species of commonly consumed aquatic animals, and phenotyping and genotyping of K. oxytoca Isolates. Antibiotics, 10.","DOI":"10.3390\/antibiotics10101235"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"105904","DOI":"10.1016\/j.mimet.2020.105904","article-title":"An improved method for MALDI-TOF analysis of wine-associated yeasts","volume":"172","author":"Zhang","year":"2020","journal-title":"J. Microbiol. Methods"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Caneiras, C., Lito, L., Melo-Cristino, J., and Duarte, A. (2019). Community- and hospital-acquired Klebsiella pneumoniae urinary tract infections in portugal: Virulence and antibiotic resistance. Microorganisms, 7.","DOI":"10.3390\/microorganisms7050138"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.patbio.2012.10.004","article-title":"Virulence profiles and antibiotic susceptibility patterns of Klebsiella pneumoniae strains isolated from different clinical specimens","volume":"61","author":"Messai","year":"2013","journal-title":"Pathol. Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4049","DOI":"10.1128\/JCM.01076-06","article-title":"Emergence and outbreaks of CTX-M beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae strains in a Tunisian hospital","volume":"44","author":"Mamlouk","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1111\/j.1574-6968.2008.01208.x","article-title":"Evaluation of a multiplex PCR for detection of serotypes K1, K2 and K5 in Klebsiella sp. and comparison of isolates within these serotypes","volume":"284","author":"Turton","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"867","DOI":"10.18388\/abp.2015_1148","article-title":"Klebsiella pneumoniae: Characteristics of carbapenem resistance and virulence factors","volume":"62","author":"Candan","year":"2015","journal-title":"Acta Biochim. Pol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"289","DOI":"10.3389\/fmicb.2018.00289","article-title":"Phenotypic and genotypic characterization of Klebsiella pneumoniae isolated from retail foods in China","volume":"9","author":"Zhang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"27338","DOI":"10.1007\/s11356-019-05287-8","article-title":"Virulence, antimicrobial and heavy metal tolerance, and genetic diversity of Vibrio cholerae recovered from commonly consumed freshwater fish","volume":"26","author":"Xu","year":"2019","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5224","DOI":"10.1128\/JCM.41.11.5224-5226.2003","article-title":"Evaluation of genotyping large numbers of Escherichia coli isolates by enterobacterial repetitive intergenic consensus-PCR","volume":"41","author":"Meacham","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"3981","DOI":"10.2147\/IDR.S371477","article-title":"Clinical and microbiological characteristics of Klebsiella pneumoniae bloodstream infection in a Chinese hospital: Hypervirulent and multiclonal","volume":"15","author":"Sheng","year":"2022","journal-title":"Infect. Drug Resist."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1186\/s13742-015-0069-2","article-title":"Erratum: SOAPdenovo2: An empirically improved memory-efficient short-read de novo assembler","volume":"4","author":"Luo","year":"2015","journal-title":"Gigascience"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1093\/bioinformatics\/btm009","article-title":"Identifying bacterial genes and endosymbiont DNA with Glimmer","volume":"23","author":"Delcher","year":"2007","journal-title":"Bioinformatics"},{"key":"ref_47","first-page":"1","article-title":"tRNAscan-SE: Searching for tRNA genes in genomic sequences","volume":"Volume 1962","author":"Chan","year":"2019","journal-title":"Gene Prediction"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Xu, D., Peng, X., Xie, L., and Chen, L. (2022). Survival and genome diversity of Vibrio parahaemolyticus isolated from edible aquatic animals. Diversity, 14.","DOI":"10.3390\/d14050350"},{"key":"ref_49","first-page":"D517","article-title":"CARD 2020: Antibiotic resistome surveillance with the comprehensive antibiotic resistance database","volume":"48","author":"Alcock","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2898","DOI":"10.1128\/aem.61.8.2898-2904.1995","article-title":"Enterobacterial repetitive intergenic consensus sequences and the PCR to generate fingerprints of genomic DNAs from Vibrio cholerae O1, O139, and non-O1 strains","volume":"61","author":"Rivera","year":"1995","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1111\/jam.15166","article-title":"Epidemiology of resistance of carbapenemase-producing Klebsiella pneumoniae to ceftazidime-avibactam in a Chinese hospital","volume":"132","author":"Chen","year":"2022","journal-title":"J. Appl. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1198","DOI":"10.1056\/NEJMoa1306801","article-title":"Emerging Infections Program Healthcare-Associated Infections and Antimicrobial Use Prevalence Survey Team. Multistate point-prevalence survey of health care-associated infections","volume":"370","author":"Magill","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Barati, A., Ghaderpour, A., Chew, L.L., Bong, C.W., Thong, K.L., Chong, V.C., and Chai, L.C. (2016). Isolation and characterization of aquatic-borne Klebsiella pneumoniae from tropical estuaries in Malaysia. Int. J. Environ. Res. Public Health, 13.","DOI":"10.3390\/ijerph13040426"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"174","DOI":"10.5398\/tasj.2018.41.3.174","article-title":"Detection of blaTEM gene of Klebsiella pneumoniae isolated from swab of food-producing animals in East Java","volume":"41","author":"Effendi","year":"2018","journal-title":"Trop. Anim. Sci. J."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e12738","DOI":"10.1111\/jfs.12738","article-title":"Occurrence and genetic diversity of ESBL producing Klebsiella species isolated from livestock and livestock products","volume":"40","author":"Bobbadi","year":"2020","journal-title":"J. Food Saf."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2216","DOI":"10.1111\/1462-2920.12786","article-title":"Growth promotion and gut microbiota: Insights from antibiotic use","volume":"17","year":"2015","journal-title":"Environ. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.adaj.2017.11.034","article-title":"Assessment of inappropriate antibiotic prescribing among a large cohort of general dentists in the United States","volume":"149","author":"Durkin","year":"2018","journal-title":"J. Am. Dent. Assoc."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.jgar.2017.04.009","article-title":"Detection of multidrug resistant (MDR) bacteria in untreated waste water disposals of hospitals in Dhaka City, Bangladesh","volume":"10","author":"Rabbani","year":"2017","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1093\/femsre\/fux013","article-title":"Klebsiella pneumoniae: A major worldwide source and shuttle for antibiotic resistance","volume":"41","author":"Kondratyeva","year":"2017","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/s13756-019-0615-2","article-title":"Clinical, microbiological, and molecular epidemiological characteristics of Klebsiella pneumoniae-induced pyogenic liver abscess in southeastern China","volume":"8","author":"Zhang","year":"2019","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1111\/iwj.13550","article-title":"Virulent and multidrug-resistant Klebsiella pneumoniae from clinical samples in Balochistan","volume":"18","author":"Fatima","year":"2021","journal-title":"Int. Wound J."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1071\/EN19115","article-title":"Liquid chromatography-tandem mass spectrometry as a fast and simple method for the determination of several antibiotics in different aqueous matrices","volume":"17","author":"Voigt","year":"2019","journal-title":"Environ. Chem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e04691","DOI":"10.1016\/j.heliyon.2020.e04691","article-title":"Heavy metal pollution in the environment and their toxicological effects on humans","volume":"6","author":"Briffa","year":"2020","journal-title":"Heliyon"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.marpolbul.2018.11.050","article-title":"Distribution and pollution assessment of heavy metals in the intertidal zone environments of typical sea areas in China","volume":"138","author":"Zhao","year":"2019","journal-title":"Mar. Pollut. Bull."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.matpr.2017.11.106","article-title":"Removal of copper by adsorption on treated laterite","volume":"5","author":"Srinivas","year":"2018","journal-title":"Mater. Today Proc."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"126729","DOI":"10.1016\/j.jtemb.2021.126729","article-title":"Hexavalent chromium: Regulation and health effects","volume":"65","author":"Alvarez","year":"2021","journal-title":"J. Trace Elem. Med. Biol."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Waluyo, L., Prihanta, W., Bachtiar, Z., and Permana, T.I. (2020, January 26). Potential bioremediation of lead (Pb) using marine microalgae Nannochloropsis oculata. Proceedings of the AIP Conference Proceedings, Malang, Indonesia.","DOI":"10.1063\/5.0002441"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"151","DOI":"10.5334\/aogh.2831","article-title":"Human health and ocean pollution","volume":"86","author":"Landrigan","year":"2020","journal-title":"Ann. Glob. Health"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"105117","DOI":"10.1016\/j.envint.2019.105117","article-title":"Heavy metal pollution and co-selection for antibiotic resistance: A microbial palaeontology approach","volume":"132","author":"Dickinson","year":"2019","journal-title":"Environ. Int."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"3012","DOI":"10.1128\/JCM.00676-11","article-title":"Emerging severe and fatal infections due to Klebsiella pneumoniae in two university hospitals in France","volume":"49","author":"Verdet","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1268","DOI":"10.1086\/652183","article-title":"The function of wzy_K1 (magA), the serotype K1 polymerase gene in Klebsiella pneumoniae cps gene cluster","volume":"201","author":"Fang","year":"2010","journal-title":"J. Infect. Dis."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1080\/21505594.2017.1317412","article-title":"Hypervirulence and hypermucoviscosity: Two different but complementary Klebsiella spp. phenotypes?","volume":"8","year":"2017","journal-title":"Virulence"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"6744","DOI":"10.1128\/IAI.68.12.6744-6749.2000","article-title":"Capsule impedes adhesion to and invasion of epithelial cells by Klebsiella pneumoniae","volume":"68","author":"Sahly","year":"2000","journal-title":"Infect Immun."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"De Majumdar, S., Yu, J., Fookes, M., McAteer, S.P., Llobet, E., Finn, S., Spence, S., Monahan, A., Monaghan, A., and Kissenpfennig, A. (2015). Elucidation of the RamA regulon in Klebsiella pneumoniae reveals a role in LPS regulation. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004627"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1128\/jb.173.2.697-703.1991","article-title":"16S ribosomal DNA amplification for phylogenetic study","volume":"173","author":"Weisburg","year":"1991","journal-title":"J. Bacteriol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"4377","DOI":"10.1128\/JCM.02316-14","article-title":"Multiplex PCR for detection of seven virulence factors and K1\/K2 capsular serotypes of Klebsiella pneumoniae","volume":"52","author":"Compain","year":"2014","journal-title":"J. Clin. Microbiol."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/24\/4058\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:42:00Z","timestamp":1760146920000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/11\/24\/4058"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,15]]},"references-count":76,"journal-issue":{"issue":"24","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["foods11244058"],"URL":"https:\/\/doi.org\/10.3390\/foods11244058","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,15]]}}}