{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:34:36Z","timestamp":1790721276756,"version":"4.1.0"},"reference-count":63,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Regione Puglia","award":["H95E19000340006"],"award-info":[{"award-number":["H95E19000340006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Due to the growing global incidence of allergy to nuts and peanuts, the need for better protection of consumers sensitive to those products is constantly increasing. The best strategy to defend them against adverse immunological reactions still remains the total removal of those products from their diet. However, nuts and peanuts traces can also be hidden in other food products, especially processed ones, such as bakery products, because of cross-contamination occurring during production. Precautionary labelling is often adopted by producers to warn allergic consumers, usually without any evaluation of the actual risk, which would require a careful quantification of nuts\/peanuts traces. In this paper, the development of a multi-target method based on liquid chromatography-tandem high resolution mass spectrometry (LC-MS, MS\/MS), able to detect traces of five nuts species (almonds, hazelnuts, walnuts, cashews and pistachios) and of peanuts in an in-house incurred bakery product (cookie) through a single analysis is described. Specifically, allergenic proteins of the six ingredients were used as the analytical targets, and the LC-MS responses of selected peptides resulting from their tryptic digestion, after extraction from the bakery product matrix, were exploited for quantification, following a bottom-up approach typical of proteomics. As a result, nuts\/peanuts could be detected\/quantified down to mg\u00b7kg\u22121 levels in the model cookie, thus opening interesting perspectives for the quantification of hidden nuts\/peanuts in bakery products and, consequently, for a more rational use of precautionary labelling.<\/jats:p>","DOI":"10.3390\/foods12040726","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T04:57:41Z","timestamp":1675832261000},"page":"726","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Multi-Target Detection of Nuts and Peanuts as Hidden Allergens in Bakery Products through Bottom-Up Proteomics and High-Resolution Mass Spectrometry"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7289-4854","authenticated-orcid":false,"given":"Anna","family":"Luparelli","sequence":"first","affiliation":[{"name":"Institute of Sciences of Food Production, National Research Council (ISPA-CNR), Via G. Amendola, 122\/O, 70126 Bari, Italy"},{"name":"Department of Chemistry, University of Bari \u201cAldo Moro\u201d, Via E. Orabona 4, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0025-3350","authenticated-orcid":false,"given":"Ilario","family":"Losito","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Bari \u201cAldo Moro\u201d, Via E. Orabona 4, 70126 Bari, Italy"},{"name":"SMART Inter-Department Research Center, University of Bari \u201cAldo Moro\u201d, Via E. Orabona 4, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1290-4544","authenticated-orcid":false,"given":"Elisabetta","family":"De Angelis","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, National Research Council (ISPA-CNR), Via G. Amendola, 122\/O, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6789-1472","authenticated-orcid":false,"given":"Rosa","family":"Pilolli","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, National Research Council (ISPA-CNR), Via G. Amendola, 122\/O, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5650-7909","authenticated-orcid":false,"given":"Linda","family":"Monaci","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, National Research Council (ISPA-CNR), Via G. Amendola, 122\/O, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.jaci.2017.11.003","article-title":"Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management","volume":"141","author":"Sicherer","year":"2018","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S28","DOI":"10.1542\/peds.2019-2461PP","article-title":"The public health impact of parent-reported childhood food allergies in the United States","volume":"144","author":"Laubach","year":"2019","journal-title":"Pediatrics"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1186\/s13601-020-00318-x","article-title":"COST Action \u201cImpARAS\u201d: What have we learnt to improve food allergy risk assessment. A summary of a 4 year networking consortium","volume":"10","author":"Verhoeckx","year":"2020","journal-title":"Clin. Transl. Allergy"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.anai.2019.01.014","article-title":"Economic burden of food allergy: A systematic review","volume":"122","author":"Bilaver","year":"2019","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/s11882-010-0112-4","article-title":"Cross-Contamination of Foods and Implications for Food Allergic Patients","volume":"10","author":"Taylor","year":"2010","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"673","DOI":"10.3389\/fimmu.2019.00673","article-title":"Food-induced anaphylaxis: Role of hidden allergens and cofactors","volume":"10","author":"Skypala","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1007\/s11882-015-0555-8","article-title":"The Prevalence of Tree Nut Allergy: A Systematic Review","volume":"15","author":"McWilliam","year":"2015","journal-title":"Curr. Allergy Asthma Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1016\/j.anai.2020.01.024","article-title":"Prevalence and natural history of tree nut allergy","volume":"124","author":"McWilliam","year":"2020","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.aller.2019.07.005","article-title":"Phenotypical characterization of tree nuts and peanut allergies in east Mediterranean children","volume":"48","author":"Cetinkaya","year":"2020","journal-title":"Allergol. Immunopathol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"41","DOI":"10.2147\/JAA.S141636","article-title":"Current perspectives on tree nut allergy: A review","volume":"11","author":"Weinberger","year":"2018","journal-title":"J. Asthma Allergy"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3794","DOI":"10.1080\/10408398.2016.1175415","article-title":"Processing effects on tree nut allergens: A review","volume":"57","author":"Vanga","year":"2017","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Ros, E., Singh, A., and O\u2019keefe, J.H. (2021). Nuts: Natural pleiotropic nutraceuticals. Nutrients, 13.","DOI":"10.3390\/nu13093269"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.molimm.2018.03.011","article-title":"Tree nut allergens","volume":"100","author":"Geiselhart","year":"2018","journal-title":"Mol. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Luparelli, A., Losito, I., De Angelis, E., Pilolli, R., Lambertini, F., and Monaci, L. (2022). Tree Nuts and Peanuts as a Source of Beneficial Compounds and a Threat for Allergic Consumers: Overview on Methods for Their Detection in Complex Food Products. Foods, 11.","DOI":"10.3390\/foods11050728"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4657","DOI":"10.1002\/jsfa.10417","article-title":"Almond allergens: Update and perspective on identification and characterization","volume":"100","author":"Zhang","year":"2020","journal-title":"J. Sci. Food Agric."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2579","DOI":"10.1080\/10408398.2013.826173","article-title":"Hazelnut allergens: Molecular characterization, detection, and clinical relevance","volume":"56","author":"Costa","year":"2016","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/cea.12267","article-title":"Walnut allergens: Molecular characterization, detection and clinical relevance","volume":"44","author":"Costa","year":"2014","journal-title":"Clin. Exp. Allergy"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s12016-016-8580-5","article-title":"Cashew Nut Allergy: Clinical Relevance and Allergen Characterisation","volume":"57","author":"Mendes","year":"2019","journal-title":"Clin. Rev. Allergy Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1080\/10408398.2017.1379947","article-title":"Pistachio nut allergy: An updated overview","volume":"59","author":"Costa","year":"2019","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.molimm.2018.04.005","article-title":"Peanut allergens","volume":"100","author":"Palladino","year":"2018","journal-title":"Mol. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1159\/000072135","article-title":"Tree nut allergens","volume":"131","author":"Roux","year":"2003","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.jaci.2007.07.024","article-title":"Current perspectives Evolutionary biology of plant food allergens","volume":"120","author":"Radauer","year":"2007","journal-title":"J. Allergy Clin. Immunol."},{"key":"#cr-split#-ref_23.1","unstructured":"(2017). European Union Commission Notice C 428\/1 of 13 July 2017 Commission Notice of 13 July 2017 relating to the provision of information on substances or products causing allergies or intolerances as listed in Annex II to Regulation"},{"key":"#cr-split#-ref_23.2","unstructured":"(EU) No 1169\/2011 of the European Parliament and of the Council on the provision of. Off. J. Eur. Union, 2017, 11-15."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/j.chroma.2017.11.039","article-title":"Liquid chromatography coupled to tandem mass spectrometry for detecting ten allergens in complex and incurred foodstuffs","volume":"1530","author":"Planque","year":"2017","journal-title":"J. Chromatogr. A"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.jaci.2013.06.042","article-title":"Allergen reference doses for precautionary labeling (VITAL 2.0): Clinical implications","volume":"133","author":"Allen","year":"2014","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1111\/all.12614","article-title":"Precautionary allergen labelling: Perspectives from key stakeholder groups","volume":"70","author":"DunnGalvin","year":"2015","journal-title":"Allergy"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.fct.2013.10.032","article-title":"Establishment of Reference Doses for residues of allergenic foods: Report of the VITAL Expert Panel","volume":"63","author":"Taylor","year":"2014","journal-title":"Food Chem. Toxicol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"150","DOI":"10.3390\/allergies1030014","article-title":"Nut Allergenicity: Effect of Food Processing","volume":"1","author":"Cuadrado","year":"2021","journal-title":"Allergies"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1097\/00130832-200406000-00018","article-title":"Food processing: Effects on allergenicity","volume":"4","author":"Maleki","year":"2004","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/j.foodchem.2015.08.119","article-title":"Insoluble and soluble roasted walnut proteins retain antibody reactivity","volume":"194","author":"Downs","year":"2016","journal-title":"Food Chem."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Gou, J., Liang, R., Huang, H., and Ma, X. (2022). Maillard Reaction Induced Changes in Allergenicity of Food. Foods, 11.","DOI":"10.3390\/foods11040530"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Barazorda-Ccahuana, H.L., Theiss-De-Rosso, V., Valencia, D.E., and G\u00f3mez, B. (2020). Heat-stable hazelnut profilin: Molecular dynamics simulations and immunoinformatics analysis. Polymers, 12.","DOI":"10.3390\/polym12081742"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"De Angelis, E., Di Bona, D., Pilolli, R., Loiodice, R., Luparelli, A., Giliberti, L., D\u2019uggento, A.M., Rossi, M.P., Macchia, L., and Monaci, L. (2022). In Vivo and In Vitro Assessment and Proteomic Analysis of the Effectiveness of Physical Treatments in Reducing Allergenicity of Hazelnut Proteins. Nutrients, 14.","DOI":"10.20944\/preprints202201.0289.v1"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.1002\/mnfr.200800485","article-title":"Influence of thermal processing on IgE reactivity to lentil and chickpea proteins","volume":"53","author":"Cuadrado","year":"2009","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/j.foodchem.2017.10.132","article-title":"Thermal processing effects on the IgE-reactivity of cashew and pistachio","volume":"245","author":"Sanchiz","year":"2018","journal-title":"Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1002\/jsfa.1748","article-title":"Impact of \u03b3-irradiation and thermal processing on the antigenicity of almond, cashew nut and walnut proteins","volume":"84","author":"Su","year":"2004","journal-title":"J. Sci. Food Agric."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"101454","DOI":"10.1016\/j.fbio.2021.101454","article-title":"The effect of roasting on peanut allergens\u2019 digestibility, allergenicity, and structure","volume":"44","author":"Zhou","year":"2021","journal-title":"Food Biosci."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Villa, C., Costa, J., and Mafra, I. (2019). Detection and quantification of milk ingredients as hidden allergens in meat products by a novel specific real-time PCR method. Biomolecules, 9.","DOI":"10.3390\/biom9120804"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2719","DOI":"10.1007\/s12161-019-01627-z","article-title":"Development of ELISA Method for Detecting Crustacean Major Allergen Tropomyosin in Processed Food Samples","volume":"12","author":"Yu","year":"2019","journal-title":"Food Anal. Methods"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"116050","DOI":"10.1016\/j.trac.2020.116050","article-title":"Recent advances and challenges in food-borne allergen detection","volume":"132","author":"Morais","year":"2020","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sena-Torralba, A., Gabald\u00f3n-Atienza, J., Cubells-G\u00f3mez, A., Casino, P., Maquieira, \u00c1., and Morais, S. (2022). Lateral Flow Microimmunoassay (LF\u00b5IA) for the Reliable Quantification of Allergen Traces in Food Consumables. Biosensors, 12.","DOI":"10.3390\/bios12110980"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"6887","DOI":"10.1080\/10408398.2021.1907736","article-title":"Advances on the rapid and multiplex detection methods of food allergens","volume":"62","author":"Xu","year":"2022","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Monaci, L., De Angelis, E., Guagnano, R., Ganci, A.P., Garaguso, I., Fiocchi, A., and Pilolli, R. (2020). Validation of a MS based proteomics method for milk and egg quantification in cookies at the lowest VITAL levels: An alternative to the use of precautionary labeling. Foods, 9.","DOI":"10.20944\/preprints202008.0630.v1"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"109256","DOI":"10.1016\/j.foodcont.2022.109256","article-title":"Optimization of a sample preparation workflow based on UHPLC-MS\/MS method for multi-allergen detection in chocolate: An outcome of the ThRAll project","volume":"143","author":"Henrottin","year":"2023","journal-title":"Food Control"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"100444","DOI":"10.1016\/j.crfs.2023.100444","article-title":"Development of a simple and reliable LC-MS\/MS method to simultaneously detect walnut and almond as specified in food allergen labelling regulations in processed foods","volume":"6","author":"Torii","year":"2023","journal-title":"Curr. Res. Food Sci."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Lu, Y., Ji, H., Chen, Y., Li, Z., and Timira, V. (2022). A systematic review on the recent advances of wheat allergen detection by mass spectrometry: Future prospects. Crit. Rev. Food Sci. Nutr., 1\u201317.","DOI":"10.1080\/10408398.2022.2101091"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.trac.2018.06.016","article-title":"Comprehensive overview and recent advances in proteomics MS based methods for food allergens analysis","volume":"106","author":"Monaci","year":"2018","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"108747","DOI":"10.1016\/j.foodres.2019.108747","article-title":"Critical review on proteotypic peptide marker tracing for six allergenic ingredients in incurred foods by mass spectrometry","volume":"128","author":"Pilolli","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"5653","DOI":"10.1007\/s00216-018-0927-8","article-title":"In house validation of a high resolution mass spectrometry Orbitrap-based method for multiple allergen detection in a processed model food","volume":"410","author":"Pilolli","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1016\/j.foodchem.2016.10.110","article-title":"Streamlining the analytical workflow for multiplex MS\/MS allergen detection in processed foods","volume":"221","author":"Pilolli","year":"2017","journal-title":"Food Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/10408398.2017.1356264","article-title":"Effects of daily food processing on allergenicity","volume":"59","author":"Cabanillas","year":"2019","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"102635","DOI":"10.1016\/j.ifset.2021.102635","article-title":"Effect of roasting duration on the solubility, structure, and IgE-binding capacity of cashew nut proteins","volume":"68","author":"Yan","year":"2021","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.fct.2015.03.005","article-title":"Food processing and allergenicity","volume":"80","author":"Verhoeckx","year":"2015","journal-title":"Food Chem. Toxicol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1007\/s12161-011-9255-8","article-title":"Effect of Processing on the Detectability of Pecan Proteins Assessed by Immunological and Proteomic Tools","volume":"5","author":"Polenta","year":"2012","journal-title":"Food Anal. Methods"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0378-4347(01)00110-4","article-title":"Stability of food allergens and allergenicity of processed foods","volume":"756","author":"Besler","year":"2001","journal-title":"J. Chromatogr. B Biomed. Sci. Appl."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.molimm.2018.03.017","article-title":"Stability of allergens","volume":"100","author":"Pekar","year":"2018","journal-title":"Mol. Immunol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.jprot.2018.11.002","article-title":"Determination of food allergens by LC-MS: Impacts of sample preparation, food matrix, and thermal processing on peptide detectability and quantification","volume":"196","author":"Korte","year":"2019","journal-title":"J. Proteom."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1208\/aapsj080120","article-title":"Effect of N-1 and N-2 residues on peptide deamidation rate in solution and solid state","volume":"8","author":"Li","year":"2006","journal-title":"AAPS J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1007\/s12161-012-9521-4","article-title":"A Rapid Size-Exclusion Solid-Phase Extraction Step for Enhanced Sensitivity in Multi-Allergen Determination in Dark Chocolate and Biscuits by Liquid Chromatography-Tandem Mass Spectrometry","volume":"6","author":"Bignardi","year":"2013","journal-title":"Food Anal. Methods"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1039\/C9AY00256A","article-title":"Simultaneous determination of major peanut allergens Ara h1 and Ara h2 in baked foodstuffs based on their signature peptides using ultra-performance liquid chromatography coupled to tandem mass spectrometry","volume":"11","author":"Zhang","year":"2019","journal-title":"Anal. Methods"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1007\/s00216-016-9384-4","article-title":"Comprehensive peptide marker identification for the detection of multiple nut allergens using a non-targeted LC-HRMS multi-method","volume":"408","author":"Korte","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"132","DOI":"10.5740\/jaoacint.17-0403","article-title":"Simultaneous analysis of multiple allergens in food products by LC-MS\/MS","volume":"101","author":"New","year":"2018","journal-title":"J. AOAC Int."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/12\/4\/726\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:27:00Z","timestamp":1760120820000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/12\/4\/726"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,7]]},"references-count":63,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["foods12040726"],"URL":"https:\/\/doi.org\/10.3390\/foods12040726","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,7]]}}}