{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:12:07Z","timestamp":1792804327022,"version":"4.5.0"},"reference-count":90,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,3,4]],"date-time":"2023-03-04T00:00:00Z","timestamp":1677888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanxi Agricultural University Youth Science and Technology Innovation Fund","award":["2016020"],"award-info":[{"award-number":["2016020"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Shanxi Agricultural University Youth Science and Technology Innovation Fund","award":["202105D121010"],"award-info":[{"award-number":["202105D121010"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, Shanxi Agricultural University","award":["2016020"],"award-info":[{"award-number":["2016020"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, Shanxi Agricultural University","award":["202105D121010"],"award-info":[{"award-number":["202105D121010"]}],"id":[{"id":"https:\/\/ror.org\/05e9f5362","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>In a previous study, we demonstrated that the hydro extract of Mao Jian Green Tea (MJGT) promotes gastrointestinal motility. In this study, the effect of MJGT ethanol extract (MJGT_EE) in treating irritable bowel syndrome with constipation (IBS-C) in a rat model constructed via maternal separation combined with an ice water stimulation was investigated. First, a successful model construction was confirmed through the determination of the fecal water content (FWC) and the smallest colorectal distension (CRD) volume. Then, the overall regulatory effects of MJGT_EE on the gastrointestinal tract were preliminarily evaluated through gastric emptying and small intestinal propulsion tests. Our findings indicated that MJGT_EE significantly increased FWC (p &lt; 0.01) and the smallest CRD volume (p &lt; 0.05) and promoted gastric emptying and small intestinal propulsion (p &lt; 0.01). Furthermore, mechanistically, MJGT_EE reduced intestinal sensitivity by regulating the expression of proteins related to the serotonin (5-hydroxytryptamine; 5-HT) pathway. More specifically, it decreased tryptophan hydroxylase (TPH) expression (p &lt; 0.05) and increased serotonin transporter (SERT) expression (p &lt; 0.05), thereby decreasing 5-HT secretion (p &lt; 0.01), activating the calmodulin (CaM)\/myosin light chain kinase (MLCK) pathway, and increasing 5-HT4 receptor (5-HT4R) expression (p &lt; 0.05). Moreover, MJGT_EE enhanced the diversity of gut microbiota, increased the proportion of beneficial bacteria, and regulated the number of 5-HT-related bacteria. Flavonoids may play the role of being active ingredients in MJGT_EE. These findings suggest that MJGT_EE could serve as a potential therapeutic pathway for IBS-C.<\/jats:p>","DOI":"10.3390\/foods12051101","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T01:35:30Z","timestamp":1678066530000},"page":"1101","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Ethanol Extract of Mao Jian Green Tea Attenuates Gastrointestinal Symptoms in a Rat Model of Irritable Bowel Syndrome with Constipation via the 5-hydroxytryptamine Signaling Pathway"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1087-9034","authenticated-orcid":false,"given":"Lei","family":"Wu","sequence":"first","affiliation":[{"name":"Institute of Molecular Science, Shanxi University, Taiyuan 030006, China"},{"name":"Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Taigu, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Gao","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Taigu, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Jin","sequence":"additional","affiliation":[{"name":"Institute of Molecular Science, Shanxi University, Taiyuan 030006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhikang","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Taigu, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xutong","family":"Qiao","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Taigu, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiting","family":"Cui","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Taigu, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0051-9235","authenticated-orcid":false,"given":"Jianhua","family":"Gao","sequence":"additional","affiliation":[{"name":"Shanxi Key Laboratory of Minor Crops Germplasm Innovation and Molecular Breeding, College of Life Sciences, Shanxi Agricultural University, Taigu, Jinzhong 030801, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9247-6334","authenticated-orcid":false,"given":"Liwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Molecular Science, Shanxi University, Taiyuan 030006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,4]]},"reference":[{"key":"ref_1","first-page":"259","article-title":"An exploration into the motivation for gluten avoidance in the absence of coeliac disease","volume":"11","author":"Harper","year":"2018","journal-title":"Gastroenterol. 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