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A silicon quantum dots (SiQD) and silver nanoclusters (AgNC) complex, namely SiQD-AgNC, was used as the dual emission fluorescence materials. Due to the fluorescence resonance energy transfer (FRET) effect between SiQD and AgNC, when the fluorescence of AgNC (610 nm) was quenched by H2S or CH3SH, the fluorescence of SiQD (468 nm) recovered, resulting in an increase of the fluorescent intensity ratio (I468\/I610). I468\/I610 showed a linear relationship with the H2S concentration within the concentration range of 1.125\u201317 \u03bcM, with a limit of detection (LOD) value of 53.6 nM. Meanwhile, I468\/I610 presented two linear relationships with the CH3SH concentration within the concentration range of 1.125\u201317 \u03bcM and 23.375\u201338.25 \u03bcM, respectively, with a LOD value of 56.5 nM. The SiQD-AgNC complex was coated on a polyvinylidene fluoride (PVDF) film to form a portable SiQD-AgNC\/PVDF film sensor. This film showed purplish red-to-cyan color changes in response to H2S and CH3SH, with LOD values of 224 nM and 233 nM to H2S and CH3SH, respectively. When the film was used to monitor beef freshness at 4 \u00b0C, its fluorescent color gradually changed from purplish red to cyan. Hence, this study presented a new ratiometric fluorescent sensor for intelligent food packaging.<\/jats:p>","DOI":"10.3390\/foods12071464","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T01:05:26Z","timestamp":1680138326000},"page":"1464","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["A Ratiometric Fluorescent Sensor Based on Silicon Quantum Dots and Silver Nanoclusters for Beef Freshness Monitoring"],"prefix":"10.3390","volume":"12","author":[{"given":"Yue","family":"Sun","sequence":"first","affiliation":[{"name":"School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China"},{"name":"Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China"},{"name":"International Joint Research Laboratory of Intelligent Agriculture and Agri-Products Processing, Jiangsu Education Department, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Zhai","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China"},{"name":"Institute of Modern Agriculture and Health Care Industry, Wencheng 325300, China"},{"name":"Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China"},{"name":"International Joint Research Laboratory of Intelligent Agriculture and Agri-Products Processing, Jiangsu Education Department, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Zou","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China"},{"name":"Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China"},{"name":"International Joint Research Laboratory of Intelligent Agriculture and Agri-Products Processing, Jiangsu Education Department, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiyong","family":"Shi","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China"},{"name":"Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China"},{"name":"International Joint Research Laboratory of Intelligent Agriculture and Agri-Products Processing, Jiangsu Education Department, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China"},{"name":"Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China"},{"name":"International Joint Research Laboratory of Intelligent Agriculture and Agri-Products Processing, Jiangsu Education Department, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Li","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China"},{"name":"Yixing Institute of Food and Biotechnology Co., Ltd., Yixing 214200, China"},{"name":"International Joint Research Laboratory of Intelligent Agriculture and Agri-Products Processing, Jiangsu Education Department, Zhenjiang 212013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dirpan, A., and Hidayat, S.H. 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