{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T14:56:59Z","timestamp":1794322619868,"version":"4.9.0"},"reference-count":67,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T00:00:00Z","timestamp":1680739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Star of Science and Technology of Dalian","award":["2020RQ074"],"award-info":[{"award-number":["2020RQ074"]}]},{"name":"the Star of Science and Technology of Dalian","award":["CARS-50"],"award-info":[{"award-number":["CARS-50"]}]},{"name":"the Star of Science and Technology of Dalian","award":["2021TSGC1263"],"award-info":[{"award-number":["2021TSGC1263"]}]},{"name":"the Star of Science and Technology of Dalian","award":["JL202009"],"award-info":[{"award-number":["JL202009"]}]},{"name":"the project of China Agriculture Research System","award":["2020RQ074"],"award-info":[{"award-number":["2020RQ074"]}]},{"name":"the project of China Agriculture Research System","award":["CARS-50"],"award-info":[{"award-number":["CARS-50"]}]},{"name":"the project of China Agriculture Research System","award":["2021TSGC1263"],"award-info":[{"award-number":["2021TSGC1263"]}]},{"name":"the project of China Agriculture Research System","award":["JL202009"],"award-info":[{"award-number":["JL202009"]}]},{"name":"Innovation Ability Improvement Project of Science and Technology smes in Shandong Province","award":["2020RQ074"],"award-info":[{"award-number":["2020RQ074"]}]},{"name":"Innovation Ability Improvement Project of Science and Technology smes in Shandong Province","award":["CARS-50"],"award-info":[{"award-number":["CARS-50"]}]},{"name":"Innovation Ability Improvement Project of Science and Technology smes in Shandong Province","award":["2021TSGC1263"],"award-info":[{"award-number":["2021TSGC1263"]}]},{"name":"Innovation Ability Improvement Project of Science and Technology smes in Shandong Province","award":["JL202009"],"award-info":[{"award-number":["JL202009"]}]},{"name":"the Project of Education Department of Liaoning Province","award":["2020RQ074"],"award-info":[{"award-number":["2020RQ074"]}]},{"name":"the Project of Education Department of Liaoning Province","award":["CARS-50"],"award-info":[{"award-number":["CARS-50"]}]},{"name":"the Project of Education Department of Liaoning Province","award":["2021TSGC1263"],"award-info":[{"award-number":["2021TSGC1263"]}]},{"name":"the Project of Education Department of Liaoning Province","award":["JL202009"],"award-info":[{"award-number":["JL202009"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Fucoidanase is an unstable enzyme with high specificity that requires a large about of time to screen it from microorganisms. In this study, enzymatic hydrolysis was used to produce low-molecular-weight fucoidan from microorganisms via the degradation of high-molecular-weight fucoidan without damage to the sulfate esterification structure of oligosaccharide. The microbial strain HN-25 was isolated from sea mud and was made to undergo mutagenicity under ultraviolet light. Fucoidanase was extracted via ultrasonication and its enzymatic activity was improved via optimization of the ultrasonic conditions. The enzymatic properties and degradation efficiency of fucoidanase were characterized. The microbial strain HN-25 is a Gram-negative aerobic and rod-shaped-cell bacterium, and therefore was identified as Cobetia amphilecti via 16s rDNA. The results proved that fucoidanase is a hydrolytic enzyme with a molecular weight of 35 kDa and with high activity and stability at 30 \u00b0C and pH 8.0. The activity of fucoidanase was significantly enhanced by sodium and calcium ions and inhibited by a copper ion and ethylenediaminetetraacetate (EDTA). There was a significant decrease in the molecular weight of fucoidan after enzymatic hydrolysis. The low-molecular-weight fuicodan was divided into four fractions, mainly concentrated at F3 (20~10 kDa) and F4 (\u22646 kDa). These consequences suggest that fucoidanase obtained from Cobetia amphilecti is stable and efficient and could be a good tool in the production of bioactive compounds.<\/jats:p>","DOI":"10.3390\/foods12071555","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T08:41:52Z","timestamp":1680770512000},"page":"1555","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Purification and Characterization of the Enzyme Fucoidanase from Cobetia amphilecti Utilizing Fucoidan from Undaria pinnatifida"],"prefix":"10.3390","volume":"12","author":[{"given":"Shu","family":"Liu","sequence":"first","affiliation":[{"name":"Colleage of Food Science and Technology, Huazhong Agriculture University, Wuhan 430070, China"},{"name":"Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R and D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiukuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R and D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenwen","family":"Shao","sequence":"additional","affiliation":[{"name":"Qingdao Seawit Life Science Co., Ltd., Qingdao 370200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Liu","sequence":"additional","affiliation":[{"name":"Bureau of Science and Technology of Qingdao West Area, Qingdao 266555, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunhai","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R and D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Ren","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R and D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yang","sequence":"additional","affiliation":[{"name":"Colleage of Food Science and Technology, Huazhong Agriculture University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R and D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.ijbiomac.2018.12.197","article-title":"In vitro and in vivo immunomodulatory effects of fucoidan compound agents","volume":"127","author":"Peng","year":"2019","journal-title":"Int. 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