{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:51:10Z","timestamp":1791582670410,"version":"4.3.4"},"reference-count":51,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T00:00:00Z","timestamp":1681862400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shanghai Education Development Foundation","award":["15CG54"],"award-info":[{"award-number":["15CG54"]}]},{"name":"Shanghai Municipal Education Commission","award":["15CG54"],"award-info":[{"award-number":["15CG54"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Efficient methods for monitoring sulfonamides (SAs) in water and animal-source foods are of great importance to achieve environmental safety and protect human health. Here, we demonstrate a reusable and label-free electrochemical sensor for the rapid and sensitive detection of sulfamethizole based on an electropolymerized molecularly imprinted polymer (MIP) film as the recognition layer. To achieve effective recognition, monomer screening among four kinds of 3-substituted thiophenes was performed by computational simulation and subsequent experimental evaluation, and 3-thiopheneethanol was finally selected. MIP synthesis is very fast and green, and can be in situ fabricated on the transducer surface within 30 min in an aqueous solution. The preparation process of the MIP was characterized by electrochemical techniques. Various parameters affecting MIP fabrication and its recognition response were investigated in detail. Under optimized experimental conditions, good linearity in the range of 0.001\u221210 \u03bcM and a low determination limit of 0.18 nM were achieved for sulfamethizole. The sensor showed excellent selectivity, which can distinguish between structurally similar SAs. In addition, the sensor displayed good reusability and stability. Even after 7 days of storage, or being reused 7 times, higher than 90% of the initial determination signals were retained. The practical applicability of the sensor was also demonstrated in spiked water and milk samples at the nM determination level with satisfactory recoveries. Compared to relevant methods for SAs, this sensor is more convenient, rapid, economical, and eco-friendly, and had comparable or even higher sensitivity, which offered a simple and efficient method for SA detection.<\/jats:p>","DOI":"10.3390\/foods12081693","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T03:20:39Z","timestamp":1681874439000},"page":"1693","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Rapid and Sensitive Detection of Sulfamethizole Using a Reusable Molecularly Imprinted Electrochemical Sensor"],"prefix":"10.3390","volume":"12","author":[{"given":"Jie","family":"Kong","sequence":"first","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixin","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjian","family":"Miao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1750-6433","authenticated-orcid":false,"given":"Xiaojun","family":"Bian","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China"},{"name":"Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.scitotenv.2019.07.259","article-title":"Sulfate radical-based oxidation of the antibiotics sulfamethoxazole, sulfisoxazole, sul-fathiazole, and sulfamethizole: The role of five-membered heterocyclic rings","volume":"692","author":"Zhou","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"7958","DOI":"10.1021\/acs.analchem.9b01998","article-title":"Rapid and Automated Detection of Six Contaminants in Milk Using a Centrifugal Microfluidic Platform with Two Rotation Axes","volume":"91","author":"Chen","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8603","DOI":"10.1021\/acs.analchem.1c01530","article-title":"Improving SERS Sensitivity toward Trace Sulfonamides: The Key Role of Trade-Off Interfacial Interactions among the Target Molecules, Anions, and Cations on the SERS Active Surface","volume":"93","author":"Zhou","year":"2021","journal-title":"Anal. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4402","DOI":"10.1002\/jssc.202100591","article-title":"Determination of sulfonamides in milk and egg samples by HPLC with mesoporous polymelamine-formaldehyde as magnetic solid-phase extraction adsorbent","volume":"44","author":"Liao","year":"2021","journal-title":"Sep. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1080\/19440049.2019.1705399","article-title":"A multi-class multi-residue method for the analysis of polyether ionophores, tetracyclines and sulfonamides in multi-matrices of animal and aquaculture fish tissues by ultra-high performance liquid chromatography tandem mass spectrometry","volume":"37","author":"Abafe","year":"2020","journal-title":"Food Addit. Contam. A"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.foodchem.2018.04.018","article-title":"Simultaneous determination of multi-residue and multi-class antibiotics in aquaculture shrimps by UPLC-MS\/MS","volume":"260","author":"Saxena","year":"2018","journal-title":"Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.talanta.2019.03.093","article-title":"7-(Diethylamino)coumarin-3-carboxylic acid as derivatization reagent for 405 nm laser-induced fluorescence detection: A case study for the analysis of sulfonamides by capillary electrophoresis","volume":"201","author":"Wu","year":"2019","journal-title":"Talanta"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"108256","DOI":"10.1016\/j.foodcont.2021.108256","article-title":"Rapid quantitative detection for multiple antibiotics in honey using a quantum dot microsphere immunochromatographic strip","volume":"130","author":"Li","year":"2021","journal-title":"Food Control"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s00604-018-3105-y","article-title":"Galvanicdisplacement-induced codeposition of reduced-graphene-oxide\/silver on alloy fibers for non-destructive SPME@SERS analysis of antibiotics","volume":"186","author":"Cui","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"8607","DOI":"10.1021\/acs.analchem.0c01831","article-title":"Superparamagnetic Nanostructures for Split-Type and Competitive-Mode Photoelectrochemical Aptasensing","volume":"92","author":"Li","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"037505","DOI":"10.1149\/2.0052003JES","article-title":"Review-Electrochemistry\u2019s Potential to Reach the Ultimate Sensitivity in Measurement Science","volume":"167","author":"Goines","year":"2020","journal-title":"Electrochem. Soc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"357","DOI":"10.3390\/chemosensors10090357","article-title":"Polyethylenimine-Based Electrochemical Sensor for the Determination of Caffeic Acid in Aromatic Herbs","volume":"10","author":"Zamarchi","year":"2022","journal-title":"Chemosensors"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e202106493","DOI":"10.1002\/anie.202106493","article-title":"Fighting Antibiotic-Resistant Bacteria: Promising Strategies Orchestrated by Molecularly Imprinted Polymers","volume":"61","author":"Bui","year":"2022","journal-title":"Angew. Chem. Int. Ed."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1007\/s10965-021-02714-8","article-title":"Synthesis and characterization of an innovative molecular imprinted polymers based on CdTe QDs fluorescence sensing for selective detection of sulfadimidine","volume":"28","author":"Yang","year":"2021","journal-title":"J. Polym. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"112944","DOI":"10.1016\/j.bios.2020.112944","article-title":"Novel chloramphenicol sensor based on aggregation-induced electrochemiluminescence and nanozyme amplification","volume":"176","author":"Li","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"108638","DOI":"10.1016\/j.foodcont.2021.108638","article-title":"A novel molecularly imprinted polymer composite based on polyaniline nanoparticles as sensitive sensors for parathion detection in the field","volume":"133","author":"Liang","year":"2022","journal-title":"Food Control"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"129229","DOI":"10.1016\/j.foodchem.2021.129229","article-title":"A highly-sensitive and selective antibody-like sensor based on molecularly imprinted poly(L-arginine) on COOH-MWCNTs for electrochemical recognition and detection of deoxynivalenol","volume":"350","author":"Li","year":"2021","journal-title":"Food Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1021\/acsmaterialslett.2c00830","article-title":"Functionally Imprinted Orthorhombic WO3 center dot H2O Nanoplates for Ultrasensitive Photoelectrochemical Sensing with Excellent Selectivity","volume":"4","author":"Xu","year":"2022","journal-title":"ACS Mater. Lett."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"138058","DOI":"10.1016\/j.cej.2022.138058","article-title":"Templating precise multiple binding sites: Promising molecularly imprinted membrane orchestrated by sequential surface imprinting strategy","volume":"450","author":"Gao","year":"2022","journal-title":"Chem. Eng. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/j.jelechem.2018.04.036","article-title":"Electrochemical magnetic imprinted sensor based on MWCNTs@CS\/CTABr surfactant composites for sensitive sensing of diethylstilbestrol","volume":"818","author":"Zhao","year":"2018","journal-title":"J. Electroanal. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.bios.2018.12.002","article-title":"A robust electrochemical sensing of molecularly imprinted polymer prepared by using bifunctional monomer and its application in detection of cypermethrin","volume":"127","author":"Li","year":"2019","journal-title":"Biosens. Bioelectron."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1002\/elan.201800678","article-title":"Electrosynthesis of Molecularly Imprinted Poly-o-phenylenediamine on MWCNT Modified Electrode for Selective Determination of Meldonium","volume":"31","author":"Guney","year":"2019","journal-title":"Electroanalysis"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1007\/s00604-021-04812-2","article-title":"Determination of patulin using dual-dummy templates imprinted electrochemical sensor with PtPd decorated N-doped porous carbon for amplification","volume":"188","author":"Hu","year":"2021","journal-title":"Microchim. Acta"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.ijbiomac.2019.07.196","article-title":"Synthesis of chitosan based molecularly imprinted polymer for pipette-tip solid phase extraction of Rhodamine B from chili powder samples","volume":"139","author":"Bagheri","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"123158","DOI":"10.1016\/j.talanta.2021.123158","article-title":"Application of thymine-based nucleobase-modified acrylamide as a functional co-monomer in electropolymerised thin-film molecularly imprinted polymer (MIP) for selective protein (haemoglobin) binding","volume":"240","author":"Turner","year":"2022","journal-title":"Talanta"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.progpolymsci.2015.04.009","article-title":"Functionalized polythiophenes: Recognition materials for chemosensors and biosensors of superior sensitivity, selectivity, and detectability","volume":"47","author":"Huynh","year":"2015","journal-title":"Prog. Polym. Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"128600","DOI":"10.1016\/j.snb.2020.128600","article-title":"Sulfamethizole-imprinted polymer on screen-printed electrodes: Towards the design of a portable environmental sensor","volume":"320","author":"Ayankojo","year":"2020","journal-title":"Sens. Actuators B-Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"127376","DOI":"10.1016\/j.foodchem.2020.127376","article-title":"Ultrasensitive determination of sulfathiazole using a molecularly imprinted electrochemical sensor with CuS microflowers as an electron transfer probe and Au@COF for signal amplification","volume":"332","author":"Sun","year":"2020","journal-title":"Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1007\/s00604-018-3079-9","article-title":"A Cu(II)-anchored unzipped covalent triazine framework with peroxidase-mimicking properties for molecular imprinting-based electrochemiluminescent detection of sulfaquinoxaline","volume":"185","author":"Ma","year":"2018","journal-title":"Microchim. Acta"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1021\/acs.analchem.8b04314","article-title":"Facile Preparation of a Bacteria Imprinted Artificial Receptor for Highly Selective Bacterial Recognition and Label-Free Impedimetric Detection","volume":"91","author":"Wu","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1016\/j.jcis.2021.04.133","article-title":"An innovative and simple all electrochemical approach to functionalize electrodes with a carbon nanotubes\/polypyrrole molecularly imprinted nanocomposite and its application for sulfamethoxazole analysis","volume":"599","author":"Turco","year":"2021","journal-title":"J. Colloid Interface Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"122411","DOI":"10.1016\/j.talanta.2021.122411","article-title":"Polydopamine molecularly imprinted polymer coated on a biomimetic iron-based metal-organic framework for highly selective fluorescence detection of metronidazole","volume":"232","author":"Zhang","year":"2021","journal-title":"Talanta"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1016\/j.apsusc.2015.09.115","article-title":"Morphology and electrical properties of electrochemically synthesized pyrrole-formyl pyrrole copolymer","volume":"357","author":"Gholami","year":"2015","journal-title":"Appl. Surf. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1007\/s10854-013-1580-9","article-title":"Investigations on structural, optical and electrochemical properties of blue luminescence SnO2 nanoparticles","volume":"25","author":"Baraneedharan","year":"2014","journal-title":"Mater. Sci.-Mater. Electron."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.trac.2017.08.012","article-title":"Trends in electrochemical impedance spectroscopy involving nanocomposite transducers: Characterization, architecture surface and bio-sensing","volume":"97","author":"Munoz","year":"2017","journal-title":"TrAc-Trends Anal. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"113089","DOI":"10.1016\/j.bios.2021.113089","article-title":"A new molecular imprinted PEDOT glassy carbon electrode for carbamazepine detection","volume":"180","author":"Hammoud","year":"2021","journal-title":"Biosens. Bioelectron."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"132829","DOI":"10.1016\/j.foodchem.2022.132829","article-title":"Molecularly imprinted sensing platform based on electrochemically modified graphite paper for efficient detection of 3-monochloropropane-1,2-diol","volume":"386","author":"Chi","year":"2022","journal-title":"Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"25972","DOI":"10.1021\/acsami.2c02474","article-title":"Electropolymerized Molecularly Imprinted Polymer Synthesis Guided by an Integrated Data-Driven Framework for Cortisol Detection","volume":"14","author":"Dykstra","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"8463","DOI":"10.3390\/s8128463","article-title":"Voltammetric Behaviour of Sulfamethoxazole on Electropolymerized-Molecularly Imprinted Overoxidized Polypyrrole","volume":"8","author":"Ozkorucuklu","year":"2008","journal-title":"Sensors"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4433","DOI":"10.1039\/D2AN01122K","article-title":"Reusable and universal impedimetric sensing platform for the rapid and sensitive detection of pathogenic bacteria based on bacteria-imprinted polythiophene film","volume":"147","author":"Wang","year":"2022","journal-title":"Analyst"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1039\/C7AY02283B","article-title":"Development of molecularly imprinted photonic polymers for sensing of sulfonamides in egg white","volume":"10","author":"Zhang","year":"2018","journal-title":"Anal. Methods"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1016\/j.snb.2017.09.164","article-title":"Preparation and characterization of molecularly imprinted mussel inspired film as antifouling and selective layer for electrochemical detection of sulfamethoxazole","volume":"255","author":"Turco","year":"2018","journal-title":"Sens. Actuators B-Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/j.snb.2019.02.079","article-title":"Ni(OH)2 nanoarrays based molecularly imprinted polymer electrochemical sensor for sensitive detection of sulfapyridine","volume":"287","author":"Liu","year":"2019","journal-title":"Sens. Actuators B-Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1002\/elan.201900099","article-title":"Molecularly Imprinted Polyresorcinol Based Capacitive Sensor for Sulphanilamide Detection","volume":"31","author":"Prusty","year":"2019","journal-title":"Electroanalysis"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1007\/s00604-019-4072-7","article-title":"Net-like mesoporous carbon nanocomposites for magnetic solid-phase extraction of sulfonamides prior to their quantitation by UPLC-HRMS","volume":"187","author":"Di","year":"2020","journal-title":"Microchim. Acta"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"15665","DOI":"10.1002\/anie.202004239","article-title":"Robust Atomistic Modeling of Materials, Organometallic, and Biochemical Systems","volume":"59","author":"Spicher","year":"2020","journal-title":"Angew. Chem. Int. Edit."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7169","DOI":"10.1039\/C9CP06869D","article-title":"Automated exploration of the low-energy chemical space withfast quantum chemical methods","volume":"22","author":"Pracht","year":"2020","journal-title":"Phys. Chem. Chem. Phys."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1021\/acs.jctc.9b00143","article-title":"Exploration of Chemical Compound, Conformer, and Reaction Space with Meta-Dynamics Simulations Based on Tight-Binding Quantum Chemical Calculations","volume":"15","author":"Grimme","year":"2019","journal-title":"J. Chem. Theory Comput."},{"key":"ref_49","unstructured":"Lu, T. (2022, December 18). Molclus Program, Version 1.9. Available online: http:\/\/www.keinsci.com\/research\/molclus.html."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Neese, F. (2022, December 18). Software Update: The ORCA Program System, Version 4.0, WIREs ComputationalMolecular Science 8(1) (2017). Available online: https:\/\/scirp.org\/reference\/referencespapers.aspx?referenceid=3028762.","DOI":"10.1002\/wcms.1327"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.snb.2018.10.055","article-title":"Novel three-dimensionalelectrochemical sensor with dual signal amplification based on MoS2 nanosheets and high-conductiveNH2-MWCNT@COF for sulfamerazine determination","volume":"281","author":"Sun","year":"2019","journal-title":"Sens. Actuat. B-Chem."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/12\/8\/1693\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:18:43Z","timestamp":1760123923000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/12\/8\/1693"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,19]]},"references-count":51,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["foods12081693"],"URL":"https:\/\/doi.org\/10.3390\/foods12081693","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,19]]}}}