{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T10:37:44Z","timestamp":1785148664324,"version":"3.55.0"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T00:00:00Z","timestamp":1682208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Nowadays, the search for food products that promote consumers\u2019 health has gained interest, and dairy by-products, due to their biological quality, could have a prominent position among products with health benefits. However, little is known about their activity on cancer cells. This study aimed to provide evidence about the effect of ovine colostrum and milk whey on K562 cells, a model of the human chronic myeloid leukemia cell line. The exposure of K562 cells to a single administration of sheep by-products at different concentrations for three days and three treatments for three days was carried out. Using a flow cytometric approach, we found that CD235a expression remained stable in the cells exposed to ovine whey (milk and colostrum) at concentrations ranging from 1 ng\/mL to 100 \u03bcg\/mL, after three days from one or three administrations, respectively. A significant reduction in fluorescent cells was observed in the populations exposed to 1 mg\/mL of both milk and colostrum at the same time points. In these conditions, the size and granularity of the leukemic cells also changed, with a substantial reduction in the number of actively dividing cells in the S phase of the cell cycle. This phenomenon was highlighted by the Annexin V\/PI cytofluorimetric test, which is able to provide quantitative results regarding the population of cells in early or late apoptosis or necrotic cells after exposure to a single dose or three doses of colostrum or sheep whey for three days, respectively. This report showed that both colostrum and milk whey were able to modify the phenotypic profile and cell cycle of the K562 cell line, inducing apoptosis at the highest concentration.<\/jats:p>","DOI":"10.3390\/foods12091752","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T02:06:11Z","timestamp":1682301971000},"page":"1752","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Utilization of Dairy By-Products as a Source of Functional and Health Compounds\u2014The Role of Ovine Colostrum and Milk Whey on Chronic Myeloid Leukemia Cells"],"prefix":"10.3390","volume":"12","author":[{"given":"Carlotta","family":"Ceniti","sequence":"first","affiliation":[{"name":"Department of Health Sciences, University \u201cMagna Gr\u00e6cia\u201d of Catanzaro, C, 88100 Catanzaro, Italy"},{"name":"Interdepartmental Center Veterinary Service for Human and Animal Health, CISVetSUA, University \u201cMagna Gr\u00e6cia\u201d, 88100 Catanzaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4413-142X","authenticated-orcid":false,"given":"Rosa Luisa","family":"Ambrosio","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Bria","sequence":"additional","affiliation":[{"name":"Laboratory of Morphology and Tissue Cell Biology, Department of Experimental and Clinical Medicine, University \u201cMagna Gr\u00e6cia\u201d, 88100 Catanzaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8294-8684","authenticated-orcid":false,"given":"Anna","family":"Di Vito","sequence":"additional","affiliation":[{"name":"Laboratory of Morphology and Tissue Cell Biology, Department of Experimental and Clinical Medicine, University \u201cMagna Gr\u00e6cia\u201d, 88100 Catanzaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6196-9358","authenticated-orcid":false,"given":"Bruno","family":"Tilocca","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University \u201cMagna Gr\u00e6cia\u201d of Catanzaro, C, 88100 Catanzaro, Italy"},{"name":"Interdepartmental Center Veterinary Service for Human and Animal Health, CISVetSUA, University \u201cMagna Gr\u00e6cia\u201d, 88100 Catanzaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aniello","family":"Anastasio","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7355-8791","authenticated-orcid":false,"given":"Domenico","family":"Britti","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University \u201cMagna Gr\u00e6cia\u201d of Catanzaro, C, 88100 Catanzaro, Italy"},{"name":"Interdepartmental Center Veterinary Service for Human and Animal Health, CISVetSUA, University \u201cMagna Gr\u00e6cia\u201d, 88100 Catanzaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6790-5734","authenticated-orcid":false,"given":"Valeria Maria","family":"Morittu","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, University \u201cMagna Gr\u00e6cia\u201d of Catanzaro, C, 88100 Catanzaro, Italy"},{"name":"Interdepartmental Center Veterinary Service for Human and Animal Health, CISVetSUA, University \u201cMagna Gr\u00e6cia\u201d, 88100 Catanzaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8314-715X","authenticated-orcid":false,"given":"Emanuela","family":"Chiarella","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Haematopoiesis and Stem Cell Biology, Department of Experimental and Clinical Medicine, University \u201cMagna Gr\u00e6cia\u201d, 88100 Catanzaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2011","DOI":"10.1080\/10408398.2018.1436038","article-title":"Exploration of collagen recovered from animal by-products as a precursor of bioactive peptides: Successes and challenges","volume":"59","author":"Fu","year":"2019","journal-title":"Crit. 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