{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T07:50:44Z","timestamp":1783756244711,"version":"3.55.0"},"reference-count":66,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003339","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["RTA2017-00027-C03-01"],"award-info":[{"award-number":["RTA2017-00027-C03-01"]}],"id":[{"id":"10.13039\/501100003339","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003339","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["AT2016-003"],"award-info":[{"award-number":["AT2016-003"]}],"id":[{"id":"10.13039\/501100003339","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>The presence of Staphylococcus aureus in six dry-cured meat-processing facilities was investigated. S. aureus was detected in 3.8% of surfaces from five facilities. The occurrence was clearly higher during processing (4.8%) than after cleaning and disinfection (1.4%). Thirty-eight isolates were typified by PFGE and MLST. Eleven sequence types (STs) were defined by MLST. ST30 (32%) and ST12 (24%) were the most abundant. Enterotoxin genes were detected in 53% of isolates. The enterotoxin A gene (sea) was present in all ST30 isolates, seb in one ST1 isolate, and sec in two ST45 isolates. Sixteen isolates harbored the enterotoxin gene cluster (egc) with four variations in the sequence. The toxic shock syndrome toxin gene (tst) was detected in 82% of isolates. Regarding antimicrobial resistance, twelve strains were susceptible to all the antibiotics tested (31.6%). However, 15.8% were resistant to three or more antimicrobials and, therefore, multidrug-resistant. Our results showed that in general, efficient cleaning and disinfection procedures were applied. Nonetheless, the presence of S. aureus with virulence determinants and resistance to antimicrobials, particularly multidrug-resistant MRSA ST398 strains, might represent a potential health hazard for consumers.<\/jats:p>","DOI":"10.3390\/foods12112161","type":"journal-article","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T16:16:04Z","timestamp":1685204164000},"page":"2161","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Staphylococcus aureus in the Processing Environment of Cured Meat Products"],"prefix":"10.3390","volume":"12","author":[{"given":"David","family":"P\u00e9rez-Boto","sequence":"first","affiliation":[{"name":"Department of Food Technology, INIA-CSIC, Carretera de La Coru\u00f1a Km 7, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9260-4017","authenticated-orcid":false,"given":"Matilde","family":"D\u2019Arrigo","sequence":"additional","affiliation":[{"name":"The Food Quality Centre, INIA-CSIC, Calle Jos\u00e9 Tudela S\/N, 42004 Soria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Garc\u00eda-Lafuente","sequence":"additional","affiliation":[{"name":"The Food Quality Centre, INIA-CSIC, Calle Jos\u00e9 Tudela S\/N, 42004 Soria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Bravo","sequence":"additional","affiliation":[{"name":"Department of Food Technology, INIA-CSIC, Carretera de La Coru\u00f1a Km 7, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida","family":"P\u00e9rez-Baltar","sequence":"additional","affiliation":[{"name":"Department of Food Technology, INIA-CSIC, Carretera de La Coru\u00f1a Km 7, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Gaya","sequence":"additional","affiliation":[{"name":"Department of Food Technology, INIA-CSIC, Carretera de La Coru\u00f1a Km 7, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Medina","sequence":"additional","affiliation":[{"name":"Department of Food Technology, INIA-CSIC, Carretera de La Coru\u00f1a Km 7, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8548-0183","authenticated-orcid":false,"given":"Juan L.","family":"Arqu\u00e9s","sequence":"additional","affiliation":[{"name":"Department of Food Technology, INIA-CSIC, Carretera de La Coru\u00f1a Km 7, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1022","DOI":"10.1111\/1541-4337.12283","article-title":"Residential Bacteria on Surfaces in the Food Industry and Their Implications for Food Safety and Quality","volume":"16","author":"Moretro","year":"2017","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_2","first-page":"63","article-title":"Staphylococcus aureus and food poisoning","volume":"2","author":"Baron","year":"2003","journal-title":"Genet. Mol. Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"EFSA (2021). The European Union One Health 2019 Zoonoses Report; 1831-4732. EFSA J., 19, e06406.","DOI":"10.2903\/j.efsa.2021.6406"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e0266220","DOI":"10.1128\/AEM.02662-20","article-title":"Staphylococcal Enterotoxin Gene Cluster: Prediction of Enterotoxin (SEG and SEI) Production and of the Source of Food Poisoning on the Basis of vSabeta Typing","volume":"87","author":"Schwendimann","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1089\/fpd.2015.1963","article-title":"The Distribution of 18 Enterotoxin and Enterotoxin-Like Genes in Staphylococcus aureus Strains from Different Sources in East China","volume":"13","author":"Cheng","year":"2016","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1089\/fpd.2019.2774","article-title":"Characterization of Oxacillin-Susceptible mecA-Positive Staphylococcus aureus from Food Poisoning Outbreaks and Retail Foods in China","volume":"17","author":"Zhang","year":"2020","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"669","DOI":"10.4049\/jimmunol.166.1.669","article-title":"egc, a highly prevalent operon of enterotoxin gene, forms a putative nursery of superantigens in Staphylococcus aureus","volume":"166","author":"Jarraud","year":"2001","journal-title":"J. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2930","DOI":"10.1128\/AEM.07487-11","article-title":"Genotypes, exotoxin gene content, and antimicrobial resistance of Staphylococcus aureus strains recovered from foods and food handlers","volume":"78","author":"Argudin","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1111\/1541-4337.12571","article-title":"Detection of toxins involved in foodborne diseases caused by Gram-positive bacteria","volume":"19","author":"Rajkovic","year":"2020","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1017\/S1466252312000187","article-title":"Methicillin-resistant staphylococci: Implications for our food supply?","volume":"13","author":"Doyle","year":"2012","journal-title":"Anim. Health Res. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.3390\/toxins2071751","article-title":"Food poisoning and Staphylococcus aureus enterotoxins","volume":"2","author":"Argudin","year":"2010","journal-title":"Toxins"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3535","DOI":"10.3168\/jds.2013-6590","article-title":"Isolation and characterization of Staphylococcus aureus strains from a Paso del Norte dairy","volume":"96","author":"Matyi","year":"2013","journal-title":"J. Dairy Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"108510","DOI":"10.1016\/j.ijfoodmicro.2020.108510","article-title":"High prevalence of multidrug resistant S. aureus-CC398 and frequent detection of enterotoxin genes among non-CC398 S. aureus from pig-derived food in Spain","volume":"320","author":"Mama","year":"2020","journal-title":"Int. J. Food Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"EFSA (2009). Assessment of the Public Health significance of meticillin resistant Staphylococcus aureus (MRSA) in animals and foods. EFSA J., 7, 993.","DOI":"10.2903\/j.efsa.2009.993"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ijfoodmicro.2008.12.007","article-title":"Prevalence of methicillin-resistant Staphylococcus aureus in meat","volume":"134","author":"Wit","year":"2009","journal-title":"Int. J. Food Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"503","DOI":"10.3389\/fmicb.2019.00503","article-title":"Occurrence and Characterization of Methicillin Resistant Staphylococcus aureus in Processed Raw Foods and Ready-to-Eat Foods in an Urban Setting of a Developing Country","volume":"10","author":"Islam","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2767","DOI":"10.3389\/fmicb.2018.02767","article-title":"Staphylococcus aureus Isolated From Retail Meat and Meat Products in China: Incidence, Antibiotic Resistance and Genetic Diversity","volume":"9","author":"Wu","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/JCM.38.3.1008-1015.2000","article-title":"Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus","volume":"38","author":"Enright","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_19","unstructured":"CLSI (2019). CLSI Supplement M100, Clinical and Laboratory Standards Institute."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e00587-20","DOI":"10.1128\/JB.00587-20","article-title":"Genome Sequencing of a Historic Staphylococcus aureus Collection Reveals New Enterotoxin Genes and Sheds Light on the Evolution and Genomic Organization of This Key Virulence Gene Family","volume":"203","author":"Dicks","year":"2021","journal-title":"J. Bacteriol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8547","DOI":"10.1128\/AEM.02045-12","article-title":"Incidence of Staphylococcus aureus and analysis of associated bacterial communities on food industry surfaces","volume":"78","author":"Gutierrez","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1624","DOI":"10.4315\/0362-028X-67.8.1624","article-title":"Microbial contamination of carcasses, meat, and equipment from an Iberian pork cutting plant","volume":"67","author":"Pala","year":"2004","journal-title":"J. Food Prot."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.fm.2007.10.001","article-title":"Microbial ecology of food contact surfaces and products of small-scale facilities producing traditional sausages","volume":"25","author":"Gounadaki","year":"2008","journal-title":"Food Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"109727","DOI":"10.1016\/j.ijfoodmicro.2022.109727","article-title":"Livestock-associated methicillin-resistant Staphylococcus aureus in Czech retailed ready-to-eat meat products","volume":"374","author":"Gelbicova","year":"2022","journal-title":"Int. J. Food Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1080\/09603123.2014.958135","article-title":"Tracing Staphylococcus aureus in small and medium-sized food-processing factories on the basis of molecular sub-species typing","volume":"25","author":"Korenova","year":"2015","journal-title":"Int. J. Environ. Health Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"763","DOI":"10.4315\/0362-028X.JFP-16-355","article-title":"A Meta-Analysis of the Global Prevalence Rates of Staphylococcus aureus and Methicillin-Resistant S. aureus Contamination of Different Raw Meat Products","volume":"80","author":"Ou","year":"2017","journal-title":"J. Food Prot."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"816","DOI":"10.3389\/fmicb.2016.00816","article-title":"Prevalence of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus in Retail Ready-to-Eat Foods in China","volume":"7","author":"Yang","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_28","first-page":"1048097","article-title":"Genetic Diversity and Virulence Factors of S. aureus Isolated from Food, Humans, and Animals","volume":"2020","year":"2020","journal-title":"Int. J. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Xie, Y., He, Y., Gehring, A., Hu, Y., Li, Q., Tu, S.I., and Shi, X. (2011). Genotypes and toxin gene profiles of Staphylococcus aureus clinical isolates from China. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0028276"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2681","DOI":"10.3389\/fmicb.2019.02681","article-title":"Antimicrobial Resistance and Virulence Gene Profiles of Methicillin-Resistant and -Susceptible Staphylococcus aureus From Food Products in Denmark","volume":"10","author":"Li","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1093\/cid\/cir181","article-title":"Multidrug-Resistant Staphylococcus aureus in US Meat and Poultry","volume":"52","author":"Waters","year":"2011","journal-title":"Clin. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.vetmic.2007.01.027","article-title":"High prevalence of methicillin resistant Staphylococcus aureus in pigs","volume":"122","author":"Spalburg","year":"2007","journal-title":"Vet. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1007\/s10096-011-1330-y","article-title":"Comparison of Staphylococcus aureus isolates associated with food intoxication with isolates from human nasal carriers and human infections","volume":"31","author":"Wattinger","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.fm.2008.12.008","article-title":"Characterization for enterotoxin production, virulence factors, and antibiotic susceptibility of Staphylococcus aureus isolates from various foods in Portugal","volume":"26","author":"Pereira","year":"2009","journal-title":"Food Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"289","DOI":"10.2478\/jvetres-2020-0036","article-title":"Characterisation of Classical Enterotoxins, Virulence Activity, and Antibiotic Susceptibility of Staphylococcus aureus Isolated from Thai Fermented Pork Sausages, Clinical Samples, and Healthy Carriers in Northeastern Thailand","volume":"64","author":"Sankomkai","year":"2020","journal-title":"J. Vet. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"M715","DOI":"10.1111\/1750-3841.13223","article-title":"Prevalence and Characteristics of Enterotoxin B-Producing Staphylococcus aureus Isolated from Food Sources: A Particular Cluster of ST188 Strains was Identified","volume":"81","author":"Song","year":"2016","journal-title":"J. Food Sci."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Jung, H.R., and Lee, Y.J. (2022). Characterization of Virulence Factors in Enterotoxin-Producing Staphylococcus aureus from Bulk Tank Milk. Animals, 12.","DOI":"10.3390\/ani12030301"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1089\/fpd.2016.2162","article-title":"Molecular Typing and Virulence Gene Profiles of Enterotoxin Gene Cluster (egc)-Positive Staphylococcus aureus Isolates Obtained from Various Food and Clinical Specimens","volume":"13","author":"Song","year":"2016","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mekhloufi, O.A., Chieffi, D., Hammoudi, A., Bensefia, S.A., Fanelli, F., and Fusco, V. (2021). Prevalence, Enterotoxigenic Potential and Antimicrobial Resistance of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Algerian Ready to Eat Foods. Toxins, 13.","DOI":"10.3390\/toxins13120835"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Chen, Q., and Xie, S. (2019). Genotypes, Enterotoxin Gene Profiles, and Antimicrobial Resistance of Staphylococcus aureus Associated with Foodborne Outbreaks in Hangzhou, China. Toxins, 11.","DOI":"10.3390\/toxins11060307"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"997","DOI":"10.3390\/toxins7030997","article-title":"Further evidence for staphylococcal food poisoning outbreaks caused by egc-encoded enterotoxins","volume":"7","author":"Johler","year":"2015","journal-title":"Toxins"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.ijfoodmicro.2006.10.050","article-title":"Characterization of Staphylococcus aureus strains associated with food poisoning outbreaks in France","volume":"115","author":"Kerouanton","year":"2007","journal-title":"Int. J. Food Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1089\/fpd.2017.2408","article-title":"Prevalence of Enterotoxin Genes in Staphylococcus aureus Isolates from Pork Production","volume":"15","author":"Zhang","year":"2018","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"197","DOI":"10.3389\/fmicb.2018.00197","article-title":"Multilocus Sequence Typing and Virulence-Associated Gene Profile Analysis of Staphylococcus aureus Isolates From Retail Ready-to-Eat Food in China","volume":"9","author":"Yang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.vetmic.2012.10.003","article-title":"Characteristics and virulence factors of livestock associated ST9 methicillin-resistant Staphylococcus aureus with a novel recombinant staphylocoagulase type","volume":"162","author":"Wan","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1086\/313461","article-title":"Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia","volume":"29","author":"Lina","year":"1999","journal-title":"Clin. Infect. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1128\/JCM.01486-08","article-title":"Clonal complexes and diversity of exotoxin gene profiles in methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from patients in a Spanish hospital","volume":"47","author":"Argudin","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4595","DOI":"10.1128\/JCM.39.12.4595-4596.2001","article-title":"Correlation of Staphylococcus aureus icaADBC genotype and biofilm expression phenotype","volume":"39","author":"Rohde","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Carrascosa, C., Raheem, D., Ramos, F., Saraiva, A., and Raposo, A. (2021). Microbial Biofilms in the Food Industry\u2014A Comprehensive Review. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18042014"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"412","DOI":"10.4315\/0362-028X.JFP-17-238","article-title":"icaA Gene of Staphylococcus aureus Responds to NaCl, Leading to Increased Biofilm Formation","volume":"81","author":"Lee","year":"2018","journal-title":"J. Food Prot."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1089\/fpd.2010.0610","article-title":"Detection, molecular characterization, and clonal diversity of methicillin-resistant Staphylococcus aureus CC398 and CC97 in Spanish slaughter pigs of different age groups","volume":"7","author":"Torres","year":"2010","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1136\/oemed-2020-106418","article-title":"Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) colonisation and infection among livestock workers and veterinarians: A systematic review and meta-analysis","volume":"78","author":"Chen","year":"2020","journal-title":"Occup. Environ. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.ijmm.2013.02.010","article-title":"Livestock associated MRSA (LA-MRSA) and its relevance for humans in Germany","volume":"303","author":"Cuny","year":"2013","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1111\/lam.12735","article-title":"Methicillin-resistant Staphylococcus aureus: A controversial food-borne pathogen","volume":"64","author":"Sergelidis","year":"2017","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.ijfoodmicro.2018.11.007","article-title":"Prevalence, antimicrobial susceptibility and characterization of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus isolated from dairy industries in north-central and north-eastern Greece","volume":"291","author":"Papadopoulos","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fm.2016.03.004","article-title":"Prevalence, antimicrobial susceptibility and molecular typing of Methicillin-Resistant Staphylococcus aureus (MRSA) in bulk tank milk from southern Italy","volume":"58","author":"Parisi","year":"2016","journal-title":"Food Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"6876","DOI":"10.3168\/jds.2018-16208","article-title":"Emergence of methicillin-resistant Staphylococcus aureus (MRSA) ST8 in raw milk and traditional dairy products in the Tizi Ouzou area of Algeria","volume":"102","author":"Titouche","year":"2019","journal-title":"J. Dairy Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1128\/AAC.50.2.505-510.2006","article-title":"Diagnostic PCR analysis of the occurrence of methicillin and tetracycline resistance genes among Staphylococcus aureus isolates from phase 3 clinical trials of tigecycline for complicated skin and skin structure infections","volume":"50","author":"Jones","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e00305-11","DOI":"10.1128\/mBio.00305-11","article-title":"Staphylococcus aureus CC398: Host adaptation and emergence of methicillin resistance in livestock","volume":"3","author":"Price","year":"2012","journal-title":"mBio"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3458","DOI":"10.1128\/JCM.41.7.3458.2003","article-title":"Detection of low-level methicillin-resistant Staphylococcus aureus with commercially available tests","volume":"41","author":"Poulsen","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"2548","DOI":"10.1128\/JCM.36.9.2548-2553.1998","article-title":"Rapid and specific detection of toxigenic Staphylococcus aureus: Use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene","volume":"36","author":"Becker","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3411","DOI":"10.1128\/JCM.37.10.3411-3414.1999","article-title":"Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates","volume":"37","author":"Monday","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"479","DOI":"10.4315\/0362-028X-63.4.479","article-title":"The detection of enterotoxins and toxic shock syndrome toxin genes in Staphylococcus aureus by polymerase chain reaction","volume":"63","author":"McLauchlin","year":"2000","journal-title":"J. Food Prot."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1128\/JCM.40.3.857-862.2002","article-title":"Detection of seg, seh, and sei genes in Staphylococcus aureus isolates and determination of the enterotoxin productivities of S. aureus isolates Harboring seg, seh, or sei genes","volume":"40","author":"Omoe","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0378-1135(02)00360-7","article-title":"Phenotypic and genotypic characterization of bovine mastitis isolates of Staphylococcus aureus for biofilm formation","volume":"92","author":"Vasudevan","year":"2003","journal-title":"Vet. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1128\/IAI.70.2.631-641.2002","article-title":"Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease","volume":"70","author":"Jarraud","year":"2002","journal-title":"Infect. Immun."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/12\/11\/2161\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:43:06Z","timestamp":1760125386000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/12\/11\/2161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,26]]},"references-count":66,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["foods12112161"],"URL":"https:\/\/doi.org\/10.3390\/foods12112161","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,26]]}}}