{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:02:01Z","timestamp":1782921721343,"version":"3.54.5"},"reference-count":44,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:00:00Z","timestamp":1692835200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Plan Project of Guangxi Province","award":["AB21196040"],"award-info":[{"award-number":["AB21196040"]}]},{"name":"Science and Technology Plan Project of Guangxi Province","award":["202119TD"],"award-info":[{"award-number":["202119TD"]}]},{"name":"Science and Technology Plan Project of Guangxi Province","award":["2023KJ124"],"award-info":[{"award-number":["2023KJ124"]}]},{"name":"Special Financial Fund of Foshan in 2023\u2013Cooperation project for high-level agricultural science and technology demonstration city construction in Guangdong Province","award":["AB21196040"],"award-info":[{"award-number":["AB21196040"]}]},{"name":"Special Financial Fund of Foshan in 2023\u2013Cooperation project for high-level agricultural science and technology demonstration city construction in Guangdong Province","award":["202119TD"],"award-info":[{"award-number":["202119TD"]}]},{"name":"Special Financial Fund of Foshan in 2023\u2013Cooperation project for high-level agricultural science and technology demonstration city construction in Guangdong Province","award":["2023KJ124"],"award-info":[{"award-number":["2023KJ124"]}]},{"name":"Agricultural competitive industry discipline team building project of Guangdong Academy of Agricultural Sciences","award":["AB21196040"],"award-info":[{"award-number":["AB21196040"]}]},{"name":"Agricultural competitive industry discipline team building project of Guangdong Academy of Agricultural Sciences","award":["202119TD"],"award-info":[{"award-number":["202119TD"]}]},{"name":"Agricultural competitive industry discipline team building project of Guangdong Academy of Agricultural Sciences","award":["2023KJ124"],"award-info":[{"award-number":["2023KJ124"]}]},{"name":"Guangdong Modern Agricultural Industry Technology System Innovation Team","award":["AB21196040"],"award-info":[{"award-number":["AB21196040"]}]},{"name":"Guangdong Modern Agricultural Industry Technology System Innovation Team","award":["202119TD"],"award-info":[{"award-number":["202119TD"]}]},{"name":"Guangdong Modern Agricultural Industry Technology System Innovation Team","award":["2023KJ124"],"award-info":[{"award-number":["2023KJ124"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["AB21196040"],"award-info":[{"award-number":["AB21196040"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["202119TD"],"award-info":[{"award-number":["202119TD"]}]},{"name":"China Agriculture Research System of MOF and MARA","award":["2023KJ124"],"award-info":[{"award-number":["2023KJ124"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Ramulus mori has been widely used in traditional Chinese medicine because of its physiological activities, including antibacterial, anti-inflammatory, and antioxidant activities. Antimicrobial properties of Ramulus mori extract have been well described. However, no information is available regarding on Ramulus mori oligosaccharides (RMOS). The aim of this study was to investigate the effects of RMOS on the growth and virulence properties of the cariogenic bacterium Streptococcus mutans. The effects of RMOS on the biofilm structure and virulence gene expression of S. mutans were also evaluated, and the results were compared with the effects of commercial prebiotic galactooligosaccharides. RMOS were found to have an antibacterial effect against S. mutans, resulting in significant reductions in acid production, lactate dehydrogenase activity, adhesion, insoluble extracellular polysaccharide production, glucosyltransferase activity, and biofilm formation in a dose-dependent manner. Moreover, the biofilm structure was visibly damaged. A quantitative real-time PCR assay revealed downregulation of virulence gene-regulated acid production, polysaccharide production, adhesion, biofilm formation, and quorum sensing. These findings suggest that RMOS may be a promising natural product for the prevention of dental caries.<\/jats:p>","DOI":"10.3390\/foods12173182","type":"journal-article","created":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T10:09:53Z","timestamp":1692871793000},"page":"3182","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antibacterial Effects of Ramulus mori Oligosaccharides against Streptococcus mutans"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6734-9686","authenticated-orcid":false,"given":"Erna","family":"Li","sequence":"first","affiliation":[{"name":"Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs\/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shipei","family":"Li","sequence":"additional","affiliation":[{"name":"Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs\/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China"},{"name":"College of Food Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs\/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Li","sequence":"additional","affiliation":[{"name":"Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs\/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daorui","family":"Pang","sequence":"additional","affiliation":[{"name":"Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs\/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiong","family":"Yang","sequence":"additional","affiliation":[{"name":"Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs\/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoling","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Food Science, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1340-9289","authenticated-orcid":false,"given":"Yuxiao","family":"Zou","sequence":"additional","affiliation":[{"name":"Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences\/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs\/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s10096-013-1993-7","article-title":"The virulence of Streptococcus mutans and the ability to form biofilms","volume":"33","author":"Jurczak","year":"2014","journal-title":"Eur. 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