{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:33:02Z","timestamp":1781109182217,"version":"3.54.1"},"reference-count":51,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T00:00:00Z","timestamp":1711411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Key Laboratory of Biological Resources and Ecological Environment (Cultivation) of Qinba","award":["SXC-2106"],"award-info":[{"award-number":["SXC-2106"]}]},{"name":"State Key Laboratory of Biological Resources and Ecological Environment (Cultivation) of Qinba","award":["2023KXJ-203"],"award-info":[{"award-number":["2023KXJ-203"]}]},{"name":"State Key Laboratory of Biological Resources and Ecological Environment (Cultivation) of Qinba","award":["23JY018"],"award-info":[{"award-number":["23JY018"]}]},{"name":"Qin Chuangyuan Team Building Project for \u2018Scientists + Engineers\u2019 in Shaanxi Province","award":["SXC-2106"],"award-info":[{"award-number":["SXC-2106"]}]},{"name":"Qin Chuangyuan Team Building Project for \u2018Scientists + Engineers\u2019 in Shaanxi Province","award":["2023KXJ-203"],"award-info":[{"award-number":["2023KXJ-203"]}]},{"name":"Qin Chuangyuan Team Building Project for \u2018Scientists + Engineers\u2019 in Shaanxi Province","award":["23JY018"],"award-info":[{"award-number":["23JY018"]}]},{"name":"Key Scientific Research Program of Shaanxi Provincial Department of Education","award":["SXC-2106"],"award-info":[{"award-number":["SXC-2106"]}]},{"name":"Key Scientific Research Program of Shaanxi Provincial Department of Education","award":["2023KXJ-203"],"award-info":[{"award-number":["2023KXJ-203"]}]},{"name":"Key Scientific Research Program of Shaanxi Provincial Department of Education","award":["23JY018"],"award-info":[{"award-number":["23JY018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Jiuqu is one of the important raw materials for brewing Chinese rice wine (Huangjiu), often known as the \u201cbone of wine\u201d. In this study, the microbial community and flavor substances of Jiuqu made with different amounts of shiitake mushroom (Lentinula edodes) were investigated through high-throughput sequencing technology and headspace gas chromatography\u2013ion migration spectroscopy (HS-GC-IMS), using traditional wheat yeast as a control. The results showed that 1593 genera and 5507 species were identified among the four types of yeast, with Aspergillus and Paecilomyces being the most dominant microorganisms at the genus level. Carbohydrate, coenzyme, and amino acid metabolism may be the main metabolic processes of the dominant microorganisms in Jiuqu. In terms of flavor, a total of 79 volatile substance monomers and some dimers were detected from four types of Jiuqu raw materials, with the main substances being 12 aldehydes, 19 ketones, 13 alcohols, 19 esters, 4 olefins, 1 acid, 3 ethers, 4 furans, 1 pyrazine, 1 pyridine, 1 triethylamine, and 1 thiazole. The correlation results indicate that Aspergillus, Lactobacillus, and Vibrio correlate significantly with the volatile flavor compounds unique to shiitake mushrooms and also have a positive effect on alcohol, esters, and furans. These results could shed light on the selection of Lentinula edodes as a fermentation starter for Huangjiu in the Qinba Mountain area.<\/jats:p>","DOI":"10.3390\/foods13071019","type":"journal-article","created":{"date-parts":[[2024,3,26]],"date-time":"2024-03-26T13:16:41Z","timestamp":1711459001000},"page":"1019","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Impact of Incorporating Shiitake Mushrooms (Lentinula edodes) on Microbial Community and Flavor Volatiles in Traditional Jiuqu"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3470-2524","authenticated-orcid":false,"given":"Jingzhang","family":"Geng","sequence":"first","affiliation":[{"name":"National Key Laboratory of Biological Resource and Ecological Protection Jointly Built by the Province and Ministry, Shaanxi University of Technology, Hanzhong 723001, China"},{"name":"School of Life Science and Technology, Xi\u2019an Jiaotong University, Xianning West Road, Xi\u2019an 710049, China"},{"name":"Collaborative Innovation Center of Bio-Resource in Qin-Ba Mountain Area, Key Laboratory of Bio-Resources of Shaanxi Province, Shaanxi University of Technology, Hanzhong 723001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8150-3460","authenticated-orcid":false,"given":"Siqiao","family":"He","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Biological Resource and Ecological Protection Jointly Built by the Province and Ministry, Shaanxi University of Technology, Hanzhong 723001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Biological Resource and Ecological Protection Jointly Built by the Province and Ministry, Shaanxi University of Technology, Hanzhong 723001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglei","family":"Tian","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Biological Resource and Ecological Protection Jointly Built by the Province and Ministry, Shaanxi University of Technology, Hanzhong 723001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9838-3905","authenticated-orcid":false,"given":"Wengang","family":"Jin","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Biological Resource and Ecological Protection Jointly Built by the Province and Ministry, Shaanxi University of Technology, Hanzhong 723001, China"},{"name":"Collaborative Innovation Center of Bio-Resource in Qin-Ba Mountain Area, Key Laboratory of Bio-Resources of Shaanxi Province, Shaanxi University of Technology, Hanzhong 723001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Yang, Y., Hu, W., Xia, Y., Mu, Z., Tao, L., Song, X., Zhang, H., Ni, B., and Ai, L. (2021). Flavor formation in Chinese rice wine (Huangjiu): Impacts of the flavor-active microorganisms, raw materials, and fermentation technology. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.636810"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.fshw.2020.02.003","article-title":"Effect of yellow rice wine on anti-aging ability in aged mice induced by D-galactose","volume":"9","author":"Liu","year":"2020","journal-title":"Food Sci. Hum. Wellness"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s00203-019-01748-3","article-title":"Bacterial succession and the dynamics of flavor compounds in the Huangjiu fermented from corn","volume":"202","author":"Ren","year":"2020","journal-title":"Arch. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.jbiosc.2020.03.006","article-title":"Influence of vacuum soaking on the brewing properties of japonica rice and the quality of Chinese rice wine","volume":"130","author":"Zhu","year":"2020","journal-title":"J. Biosci. Bioeng."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1016\/j.foodchem.2016.08.014","article-title":"Research progress on the brewing techniques of new-type rice wine","volume":"215","author":"Jiao","year":"2017","journal-title":"Food Chem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"115521","DOI":"10.1016\/j.carbpol.2019.115521","article-title":"Isolation and comparison of \u03b1- and \u03b2-D-glucans from shiitake mushrooms (Lentinula edodes) with different biological activities","volume":"229","author":"Morales","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1021\/jf990972u","article-title":"Solid-State Bioconversion of Phenolics from Cranberry Pomace and Role of Lentinus edodes b-Glucosidase","volume":"48","author":"Zheng","year":"2000","journal-title":"J. Agric. Food Chem."},{"key":"ref_8","first-page":"80","article-title":"Influence of aging time on aroma components and full-bodied flavor of fermented Lentinula edodes wine","volume":"37","author":"Wang","year":"2016","journal-title":"Food Sci."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Chen, C., Liu, Y., Tian, H., Ai, L., and Yu, H. (2020). Metagenomic analysis reveals the impact of JIUYAO microbial diversity on fermentation and the volatile profile of Shaoxing-jiu. Food Microbiol., 86.","DOI":"10.1016\/j.fm.2019.103326"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Peng, Q., Zhang, L., Huang, X., Wu, J., Cheng, Y., Xie, G., Feng, X., and Chen, X. (2023). Environmental factors affecting the diversity and composition of environmental microorganisms in the Shaoxing rice wine producing area. Foods, 12.","DOI":"10.3390\/foods12193564"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.lwt.2017.01.007","article-title":"Effect of mixed yeast starter on volatile flavor compounds in Chinese rice wine during different brewing stages","volume":"78","author":"Yang","year":"2017","journal-title":"LWT\u2014Food Sci. Technol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.fm.2018.02.002","article-title":"Microbial diversity and chemical analysis of the starters used in traditional Chinese sweet rice wine","volume":"73","author":"Cai","year":"2018","journal-title":"Food Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"101121","DOI":"10.1016\/j.fochx.2024.101121","article-title":"Effect of microbial communities on flavor profile of Hakka rice wine throughout production","volume":"21","author":"Wang","year":"2024","journal-title":"Food Chem. X"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Wang, Z., Nie, T., Zhang, H., Wang, W., Chen, H., Wang, S., and Sun, B. (2023). Correlation analysis between volatile compounds and quality attributes in pork tenderloin in response to different stir-frying processes. Foods, 12.","DOI":"10.3390\/foods12234299"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Tang, J., Tang, X., Tang, M., Zhang, X., Xu, X., and Yi, Y. (2017). Analysis of the bacterial communities in two liquors of soy sauce aroma as revealed by high-throughput sequencing of the 16S rRNA V4 hypervariable region. BioMed Res. Int., 2017.","DOI":"10.1155\/2017\/6271358"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1007\/s00284-020-01877-9","article-title":"High-throughput sequencing-based analysis of microbial diversity in rice wine koji from different areas","volume":"77","author":"Zhao","year":"2020","journal-title":"Curr. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Sun, Y., Zuo, X., Luo, R., Dang, N., Shi, D., and Liu, W. (2023). Bacterial microbiota and metabolic characteristics of traditional Zhangjiajie Jiuqu aromatic ester vinegar in China. Food Biosci., 53.","DOI":"10.1016\/j.fbio.2023.102693"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"100584","DOI":"10.1016\/j.fochx.2023.100584","article-title":"Characterization of non-volatile and volatile flavor profiles of Coregonus peled meat cooked by different methods","volume":"17","author":"Jin","year":"2023","journal-title":"Food Chem. X"},{"key":"ref_19","first-page":"56","article-title":"Effects of Polygonatum sibiricum on Microbial Community Structure and Functional Genes of Wheat Qu","volume":"45","author":"Zhang","year":"2024","journal-title":"Sci. Technol. Food Ind."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Zhang, J., Li, T., Zou, G., Wei, Y., and Qu, L. (2024). Advancements and Future Directions in Yellow Rice Wine Production Research. Fermentation, 10.","DOI":"10.3390\/fermentation10010040"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1016\/j.foodchem.2013.11.127","article-title":"Chemical and sensory profiles of makgeolli, Korean commercial rice wine, from descriptive, chemical, and volatile compound analyses","volume":"152","author":"Jung","year":"2014","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"14628","DOI":"10.1038\/s41598-021-94059-x","article-title":"Community structure and metabolic potentials of the traditional rice beer starter \u2018emao\u2019","volume":"11","author":"Narzary","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/j.ijfoodmicro.2014.03.011","article-title":"Filamentous fungal diversity and community structure associated with the solid state fermentation of Chinese Maotai-flavor liquor","volume":"179","author":"Chen","year":"2014","journal-title":"Int. J. Food Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1186\/s13568-017-0494-y","article-title":"Taxonomic identification of the thermotolerant and fast-growing fungus Lichtheimia ramosa H71D and biochemical characterization of the thermophilic xylanase LrXynA","volume":"7","author":"Campos","year":"2017","journal-title":"AMB Express"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1080\/09168451.2016.1256756","article-title":"Comparative analysis of nonvolatile and volatile metabolites in Lichtheimia ramosa cultivated in different growth media","volume":"81","author":"Chung","year":"2017","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/jam.14648","article-title":"Microbial diversity of representative traditional fermented sausages in different regions of China","volume":"130","author":"Huang","year":"2021","journal-title":"J. Appl. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Peng, Q., Meng, K., Yang, X., Zeng, H., Li, S., Chen, G., and Xie, G. (2023). Identification of geographical indication protected Huangjiu by metabonomics combined with flavor reconstruction. Food Biosci., 56.","DOI":"10.1016\/j.fbio.2023.103216"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/s13568-022-01418-6","article-title":"Microbial succession and exploration of higher alcohols-producing core bacteria in northern Huangjiu fermentation","volume":"12","author":"Yan","year":"2022","journal-title":"AMB Express"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/j.foodcont.2012.05.025","article-title":"Microbial diversity of traditional fermentation starters for Hong Qu glutinous rice wine as determined by PCR-mediated DGGE","volume":"28","author":"Lv","year":"2012","journal-title":"Food Control"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1002\/elsc.202200015","article-title":"Analysis of microbial diversity and succession during Xiaoqu Baijiu fermentation using high-throughput sequencing technology","volume":"22","author":"Wang","year":"2022","journal-title":"Eng. Life Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Liu, S.P., Zhou, Y., Ma, D.N., Zhang, S.Y., Dong, Y., Zhang, X., and Mao, J. (2023). Environment microorganism and mature daqu powder shaped microbial community formation in mechanically strong-flavor daqu. Food Biosci., 52.","DOI":"10.1016\/j.fbio.2023.102467"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"D490","DOI":"10.1093\/nar\/gkt1178","article-title":"The carbohydrate-active enzymes database (CAZy) in 2013","volume":"42","author":"Lombard","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S0959-440X(00)00253-0","article-title":"Glycoside hydrolases and glycosyltransferases: Families and functional modules","volume":"11","author":"Bourne","year":"2001","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1811","DOI":"10.1039\/c1np00045d","article-title":"Enzymatic methods for glycol (diversification\/randomization) of drugs and small molecules","volume":"28","author":"Gantt","year":"2011","journal-title":"Nat. Prod. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.biori.2017.02.001","article-title":"Structural diversity of carbohydrate esterases","volume":"1","author":"Nakamura","year":"2017","journal-title":"Biotechnol. Res. Innov."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Wu, X.Y., Jing, R.X., Chen, W.H., Geng, X.J., Li, M., Yang, F.Z., Yan, Y.Z., and Liu, Y. (2020). High-throughput sequencing of the microbial diversity of roasted-sesame-like flavored Daqu with different characteristics. 3 Biotech, 10.","DOI":"10.1007\/s13205-020-02500-1"},{"key":"ref_37","first-page":"163","article-title":"Optimization of HS-SPME-GC-MS method for analysis of volatile flavor substances from Boletus edulis Bull by response surface methodology","volume":"48","author":"Guo","year":"2023","journal-title":"China Condiment"},{"key":"ref_38","first-page":"172","article-title":"Correlation between Dominant Fungi and Variation of Flavor Compounds during High-salt Liquid-state Soy Sauce Fermentation","volume":"43","author":"Ruan","year":"2022","journal-title":"Food Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.foodchem.2013.03.018","article-title":"Contributions of non-volatile and volatile compounds to the umami taste and overall flavour of shiitake mushroom extracts and their application as flavour enhancers in cooked minced meat","volume":"141","author":"Dermiki","year":"2013","journal-title":"Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.foodres.2017.06.012","article-title":"Effect of glycine on reaction of cysteine-xylose: Insights on initial Maillard stage intermediates to develop meat flavor","volume":"99","author":"Cao","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.1021\/jf052635t","article-title":"Characterization of Aroma Compounds of Chinese \u201cWuliangye\u201d and \u201cJiannanchun\u201d Liquors by Aroma Extract Dilution Analysis","volume":"54","author":"Fan","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.foodres.2013.06.010","article-title":"Volatile biomarkers for wild mushrooms species discrimination","volume":"54","author":"Malheiro","year":"2013","journal-title":"Food Res. Int."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"4820","DOI":"10.1021\/jf0601416","article-title":"Difference in the volatile composition of pine-mushrooms (Tricholoma matsutake Sing.) according to their grades","volume":"54","author":"Cho","year":"2006","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"108786","DOI":"10.1016\/j.lwt.2019.108786","article-title":"Characterization of flavor active non-volatile compounds in chicken broth and correlated contributing constituent compounds in muscle through sensory evaluation and partial least square regression analysis","volume":"118","author":"Zhan","year":"2020","journal-title":"LWT"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"108215","DOI":"10.1016\/j.lwt.2019.05.113","article-title":"Effect of mixed moulds starters on volatile flavor compounds in rice wine","volume":"112","author":"Liu","year":"2019","journal-title":"LWT"},{"key":"ref_46","first-page":"286","article-title":"Research progress on the factors correlated with flavor quality in rice wine","volume":"48","author":"Yuan","year":"2022","journal-title":"Food Ferment. Ind."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"110421","DOI":"10.1016\/j.foodres.2021.110421","article-title":"Aroma characteristics of traditional Huangjiu produced around Winter Solstice revealed by sensory evaluation, gas chromatography-mass spectrometry and gas chromatography-ion mobility spectrometry","volume":"145","author":"Yu","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1007\/s00253-022-12291-5","article-title":"Microbial diversity in jiuqu and its fermentation features: Saccharification, alcohol fermentation and flavors generation","volume":"107","author":"Xia","year":"2023","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"111402","DOI":"10.1016\/j.lwt.2021.111402","article-title":"Characterization of flavor frame in shiitake mushrooms (Lentinula edodes) detected by HS-GC-IMS coupled with electronic tongue and sensory analysis: Influence of drying techniques","volume":"146","author":"Hou","year":"2021","journal-title":"LWT"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Panighel, A., and Flamini, R. (2014). Applications of Solid-Phase Microextraction and Gas Chromatography\/Mass Spectrometry (SPME-GC\/MS) in the Study of Grape and Wine Volatile Compounds. Molecules, 19.","DOI":"10.3390\/molecules191221291"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"100282","DOI":"10.1016\/j.rechem.2022.100282","article-title":"Analysis of aromatic components of two edible mushrooms, Phlebopus portentosus and Cantharellus yunnanensis using HS-SPME\/GC-MS","volume":"4","author":"TIian","year":"2022","journal-title":"Results Chem."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/13\/7\/1019\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:19:04Z","timestamp":1760105944000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/13\/7\/1019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,26]]},"references-count":51,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["foods13071019"],"URL":"https:\/\/doi.org\/10.3390\/foods13071019","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,26]]}}}