{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T03:26:36Z","timestamp":1787973996654,"version":"build-2784847793"},"reference-count":45,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T00:00:00Z","timestamp":1711584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"publisher","award":["GR-2018-12367064"],"award-info":[{"award-number":["GR-2018-12367064"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>In this work, Microwave-Assisted Extraction (MAE) was proposed as an alternative and environmentally friendly technique in lipidomics to study the lipid fingerprint of soft cheeses, such as mozzarella. For method development, a first step concerning an evaluation of extraction solvents was carried out via testing three different mixtures, including methanol\/ethyl acetate, isopropanol\/ethyl acetate, and ethanol\/ethyl acetate, at a 1:2 v\/v ratio. The latter was chosen as a solvent mixture for subsequent method optimization. MAE conditions, in terms of solvent volume, time, and temperature, were explored to define their effects on extraction capability through a full factorial experimental design. The best compromise to extract more lipids at the same time was obtained with 24 mL g\u22121 for solvent-to-solid ratio, 65 \u00b0C for temperature, and 18 min for time. Lipid analyses were conducted by UHPLC-Q-Orbitrap-MS associated with multivariate statistics. The developed lipidomic workflow allowed for the extraction of over 400 lipids grouped into 18 different subclasses. The results confirmed that MAE is a suitable technique for lipid extraction in the omics approach with high efficiency, even using low-cost and less toxic solvents. Moreover, a comprehensive structure characterization of extracted lipids, in terms of fatty acid composition and regiochemistry, was carried out.<\/jats:p>","DOI":"10.3390\/foods13071033","type":"journal-article","created":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T12:09:40Z","timestamp":1711627780000},"page":"1033","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Microwave-Assisted Extraction\/UHPLC-Q-Orbitrap-MS-Based Lipidomic Workflow for Comprehensive Study of Lipids in Soft Cheese"],"prefix":"10.3390","volume":"13","author":[{"given":"Maria","family":"Campaniello","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia, 20-71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Nardelli","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia, 20-71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0029-4649","authenticated-orcid":false,"given":"Rosalia","family":"Zianni","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia, 20-71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Tomaiuolo","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia, 20-71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0711-8733","authenticated-orcid":false,"given":"Oto","family":"Miedico","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia, 20-71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1728-5949","authenticated-orcid":false,"given":"Marco","family":"Iammarino","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia, 20-71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annalisa","family":"Mentana","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia, 20-71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.tifs.2020.10.045","article-title":"Mass Spectrometry-Based Lipidomics as a Powerful Platform in Foodomics Research","volume":"107","author":"Wu","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"138525","DOI":"10.1016\/j.foodchem.2024.138525","article-title":"Authentication of the Species Identity of Squid Rings Using UHPLC-Q-Orbitrap MS\/MS-Based Lipidome Fingerprinting and Chemoinformatics","volume":"442","author":"Haridas","year":"2024","journal-title":"Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Tomaiuolo, M., Nardelli, V., Mentana, A., Campaniello, M., Zianni, R., and Iammarino, M. (2023). Untargeted Lipidomics and Chemometric Tools for the Characterization and Discrimination of Irradiated Camembert Cheese Analyzed by UHPLC-Q-Orbitrap-MS. Foods, 12.","DOI":"10.3390\/foods12112198"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"126547","DOI":"10.1016\/j.foodchem.2020.126547","article-title":"Ultrasound-assisted one-phase solvent extraction coupled with liquid chromatography-quadrupole time-of-flight mass spectrometry for efficient profiling of egg yolk lipids","volume":"319","author":"Xie","year":"2020","journal-title":"Food Chem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Saini, R.K., Prasad, P., Shang, X., and Keum, Y.S. (2021). Advances in Lipid Extraction Methods\u2014A Review. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222413643"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J. Biol. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1139\/y59-099","article-title":"A rapid method of total lipid extraction and purification","volume":"37","author":"Bligh","year":"1959","journal-title":"Can. J. Biochem. Physiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.plipres.2018.06.002","article-title":"Milk lipidomics: What we know and what we don\u2019t","volume":"71","author":"Liu","year":"2018","journal-title":"Prog. Lipid Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1194\/jlr.D700041-JLR200","article-title":"Lipid extraction by methyl\u2013tert\u2013butyl ether for high\u2013throughput lipidomics","volume":"49","author":"Matyash","year":"2008","journal-title":"J. Lipid Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"12085","DOI":"10.1021\/ac403181c","article-title":"Plasma Lipidomic Profiling Method Based on Ultrasound Extraction and Liquid Chromatography Mass Spectrometry","volume":"85","author":"Pizarro","year":"2013","journal-title":"Anal. Chem."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"133542","DOI":"10.1016\/j.foodchem.2022.133542","article-title":"Optimizing accelerated solvent extraction combined with liquid chromatography-Orbitrap mass spectrometry for efficient lipid profile characterization of mozzarella cheese","volume":"394","author":"Mentana","year":"2022","journal-title":"Food Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1021\/ac9508199","article-title":"Accelerated Solvent Extraction: A Technique for Sample Preparation","volume":"68","author":"Richter","year":"1996","journal-title":"Anal. Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.foodres.2015.10.028","article-title":"Rapid microwave assisted extraction of meat lipids","volume":"78","author":"Medina","year":"2015","journal-title":"Food Res. Int."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1007\/s11306-013-0528-7","article-title":"Development and application of microwave-assisted extraction technique in biological sample preparation for small molecule analysis","volume":"9","author":"Teo","year":"2013","journal-title":"Metabolomics"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0165-9936(99)00185-5","article-title":"Microwave-assisted solvent extraction of environmental samples","volume":"19","author":"Camel","year":"2000","journal-title":"Trends Anal. Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0021-9673(00)00318-6","article-title":"Sample preparation for the analysis of flavors and off-flavors in foods","volume":"880","author":"Wilkes","year":"2000","journal-title":"J. Chromatogr. A"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.foodres.2018.07.016","article-title":"Recovery of bioactive anthocyanin pigments from Ficus carica L. peel by heat, microwave, and ultrasound based extraction techniques","volume":"113","author":"Backes","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6365","DOI":"10.3390\/molecules15096365","article-title":"Microwave Assisted Extraction of Phenolic Compounds from Four Different Spices","volume":"15","author":"Gallo","year":"2010","journal-title":"Molecules"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1002\/jsfa.5859","article-title":"Mycotoxicogenic fungal inhibition by innovative cheese cover with aromatic plants: Penicillium verrucosum inhibition in cheese by aromatic plants","volume":"93","author":"Moro","year":"2013","journal-title":"J. Sci. Food Agric."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"132778","DOI":"10.1016\/j.foodchem.2022.132778","article-title":"Nutritional and bioactive oils from salmon (Salmo salar) side streams obtained by Soxhlet and optimized microwave-assisted extraction","volume":"386","author":"Pinela","year":"2022","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Solaberrieta, I., Mellinas, A.C., Espagnol, J., Hamzaoui, M., Jim\u00e9nez, A., and Garrig\u00f3s, M.C. (2022). Valorization of Tomato Seed By-Products as a Source of Fatty Acids and Bioactive Compounds by Using Advanced Extraction Techniques. Foods, 11.","DOI":"10.3390\/foods11162408"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1600108","DOI":"10.1002\/ejlt.201600108","article-title":"Development and Validation of a Novel Microwave Assisted Extraction Method for Fish Lipids","volume":"119","author":"Costa","year":"2017","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1016\/j.jfoodeng.2006.01.089","article-title":"The Microwave-Assisted Process (MAPTM1): Extraction and Determination of Fat from Cocoa Powder and Cocoa Nibs","volume":"79","author":"ElKhori","year":"2007","journal-title":"J. Food Eng."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"104747","DOI":"10.1016\/j.jfca.2022.104747","article-title":"Comparison of conventional and green extraction methods on oil yield, physicochemical properties, and lipid compositions of pomegranate seed oil","volume":"114","author":"Liu","year":"2022","journal-title":"J. Food Compost. Anal."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1016\/j.biortech.2009.09.047","article-title":"A new combination of extraction and derivatization methods that reduces the complexity and preparation time in determining phospholipid fatty acids in solid environmental samples","volume":"101","author":"Lores","year":"2010","journal-title":"Bioresour. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"11098","DOI":"10.1016\/j.foodres.2022.110984","article-title":"Critical Review: Metabolomics in Dairy Science\u2014Evaluation of Milk and Milk Product Quality","volume":"154","author":"Suh","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"100335","DOI":"10.1016\/j.phanu.2023.100335","article-title":"Milk Polar Lipids: Untapped Potential for Pharmaceuticals and Nutraceuticals","volume":"24","author":"Pawar","year":"2023","journal-title":"PharmaNutrition"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6676","DOI":"10.1021\/acs.jafc.0c05950","article-title":"Quantification of Nonpersistent Pesticides in Small Volumes of Human Breast Milk with Ultrahigh Performance Liquid Chromatography Coupled to Tandem Mass Spectrometry","volume":"69","author":"Pedersen","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2300068","DOI":"10.1002\/jssc.202300068","article-title":"Development of Deep Eutectic Solvent\u2013Based Microwave-assisted Extraction Combined with Temperature Controlled Ionic Liquid\u2013Based Liquid Phase Microextraction for Extraction of Aflatoxins from Cheese Samples","volume":"46","author":"Kaboodi","year":"2023","journal-title":"J. Sep. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/s10337-016-3217-7","article-title":"Determination of Biogenic Amines in Cheese Using Simultaneous Derivatization and Microextraction Method Followed by Gas Chromatography\u2013Mass Spectrometry","volume":"80","author":"Mohammadi","year":"2017","journal-title":"Chromatographia"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"780151","DOI":"10.3389\/fnut.2022.780151","article-title":"Conventional and Novel Technologies in the Production of Dairy Bioactive Peptides","volume":"9","author":"Murtaza","year":"2022","journal-title":"Front. Nutr."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.idairyj.2004.06.006","article-title":"Application of a Microwave-Assisted Extraction Method for the Extraction of Organic Acids from Greek Cheeses and Sheep Milk Yoghurt and Subsequent Analysis by Ion-Exclusion Liquid Chromatography","volume":"15","author":"Papadakis","year":"2005","journal-title":"Int. Dairy J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"107447","DOI":"10.1016\/j.microc.2022.107447","article-title":"First Application of Microwave-Assisted Extraction in the Analysis of Polybrominated Diphenyl Ethers in Human Milk","volume":"179","author":"Besednik","year":"2022","journal-title":"Microchem. J."},{"key":"ref_34","first-page":"10","article-title":"Comparison of a new total fat quantification method in cheese using microwave assisted extraction (MAE) and Soxhlet","volume":"4","author":"Pombal","year":"2017","journal-title":"Braz. J. Anal. Chem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2250","DOI":"10.1007\/s12161-015-0108-8","article-title":"Microwave-Assisted Methodology Feasibility for One-Step Extraction and Transmethylation of Fatty Acids in Milk for GC-Mass Spectrometry","volume":"8","author":"Soldado","year":"2015","journal-title":"Food Anal. Methods"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1007\/s11306-018-1367-3","article-title":"Guidelines and considerations for the use of system suitability and quality control samples in mass spectrometry assays applied in untargeted clinical metabolomic studies","volume":"14","author":"Broadhurst","year":"2018","journal-title":"Metabolomics"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1007\/s11306-016-1157-8","article-title":"A relative quantitative positive\/negative ion switching method for untargeted lipidomics via high resolution LC-MS\/MS from any biological source","volume":"13","author":"Breitkopf","year":"2017","journal-title":"Metabolomics"},{"key":"ref_38","unstructured":"R Development Core Team (2024, March 16). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Version 4.1.1. Available online: http:\/\/www.R-project.org."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"103937","DOI":"10.1016\/j.chemolab.2020.103937","article-title":"mdatools\u2014R package for chemometrics","volume":"198","author":"Kucheryavskiy","year":"2020","journal-title":"Chemometr. Intell. Lab. Syst."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"W388","DOI":"10.1093\/nar\/gkab382","article-title":"MetaboAnalyst 5.0: Narrowing the gap between raw spectra and functional insights","volume":"49","author":"Pang","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Mollica, M., Trinchese, G., Cimmino, F., Penna, E., Cavaliere, G., Tudisco, R., Musco, N., Manca, C., Catapano, A., and Monda, M. (2021). Milk Fatty Acid Profiles in Different Animal Species: Focus on the Potential Effect of Selected PUFAs on Metabolism and Brain Functions. Nutrients, 13.","DOI":"10.3390\/nu13041111"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1002\/aocs.12507","article-title":"Odd chain fatty acids and odd chain phenolic lipids (alkylresorcinols) are essential for diet","volume":"98","author":"Dornan","year":"2021","journal-title":"J. Am. Oil Chem. Soc."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"6726","DOI":"10.1021\/acs.jafc.0c01604","article-title":"Comprehensive Characterization of Bovine Milk Lipids: Phospholipids, Sphingolipids, Glycolipids, and Ceramides","volume":"68","author":"Liu","year":"2020","journal-title":"J. Agric. Food Chem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1002\/bmc.3683","article-title":"Extraction, chromatographic and mass spectrometric methods for lipid analysis: Review of methods for lipid analysis","volume":"30","author":"Pati","year":"2016","journal-title":"Biomed. Chromatogr."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"111345","DOI":"10.1016\/j.foodres.2022.111345","article-title":"Milk lipids characterization in relation to different heat treatments using lipidomics","volume":"157","author":"Zhang","year":"2022","journal-title":"Food Res. Int."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/13\/7\/1033\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:19:56Z","timestamp":1760105996000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/13\/7\/1033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,28]]},"references-count":45,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["foods13071033"],"URL":"https:\/\/doi.org\/10.3390\/foods13071033","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,28]]}}}