{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T01:07:29Z","timestamp":1793754449218,"version":"4.6.1"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,4,7]],"date-time":"2024-04-07T00:00:00Z","timestamp":1712448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Veterinary Medicine Vienna"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Antimicrobial resistance is one of the major public health problems worldwide. This study aimed to detect the presence of extended-spectrum \u03b2-lactamase-(ESBL-)producing Escherichia (E.) coli in chicken meat in Istanbul, T\u00fcrkiye. Raw chicken meat samples (n = 208) were collected from different sale points and analyzed for ESBL-producing E. coli. In total, 101 (48.5%) isolates were confirmed as E. coli by PCR, of which 80\/101 (79.2%) demonstrated multiple antibiotic resistance. Resistance against amoxicillin-clavulanic acid was most frequent (87.1%). Eighteen isolates (17.8%) demonstrated phenotypical ESBL resistance, as assessed by the double disc synergy test (DDST). Isolates were tested for the presence of \u03b2-lactamase genes and mobilized colistin-resistant genes. The blaTEM group was most frequently detected (97.02%), followed by blaCTX m (45.5%), blaSHV (9.9%), and blaOXA-2 (0.9%). However, mcr genes and blaNDM,\u00a0blaKPC, blaVIM, and blaOXA-48 genes were not found in any isolate. E. coli strains were tested for biofilm formation in six different media [Nutrient broth, LB broth, Tryptone Soya broth (TSB), TSB containing 1% sucrose, TSB containing 0.6% yeast extract, and BHI]. Biofilm formation by E. coli isolates (44\/101, 43.5%) was highest in TSB with 1% sucrose. It is worth noting that all biofilm-producing isolates were found to harbor the blaTEM-1 gene, which can indicate a high level of antibiotic resistance. This is the first report about ESBL-producing E. coli in poultry meat, the exposure of consumers in Istanbul metropolitan areas, and the ability of E. coli from this region to produce biofilms.<\/jats:p>","DOI":"10.3390\/foods13071122","type":"journal-article","created":{"date-parts":[[2024,4,8]],"date-time":"2024-04-08T03:11:33Z","timestamp":1712545893000},"page":"1122","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Detection of Extended Spectrum \u00df-Lactamase-Producing Escherichia coli with Biofilm Formation from Chicken Meat in Istanbul"],"prefix":"10.3390","volume":"13","author":[{"given":"Ali","family":"Aydin","sequence":"first","affiliation":[{"name":"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, \u0130stanbul University-Cerrahpa\u015fa, 34320 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7091-5826","authenticated-orcid":false,"given":"Ali Anil","family":"Suleymanoglu","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, \u0130stanbul University-Cerrahpa\u015fa, 34320 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6268-3340","authenticated-orcid":false,"given":"Abzal","family":"Abdramanov","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sanitary Examination and Hygiene, Kazakh National Agrarian Research University, 050010 Almaty, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0153-2175","authenticated-orcid":false,"given":"Peter","family":"Paulsen","sequence":"additional","affiliation":[{"name":"Unit for Food Hygiene and Technology, Centre for Food Science and Veterinary Public Health, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna, 1210 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emek","family":"Dumen","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, \u0130stanbul University-Cerrahpa\u015fa, 34320 Istanbul, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.mib.2021.09.011","article-title":"The Potential of Using E. coli as an Indicator for the Surveillance of Antimicrobial Resistance (AMR) in the Environment","volume":"64","author":"Anjum","year":"2021","journal-title":"Curr. 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