{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T18:55:11Z","timestamp":1775069711703,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"MCIN\/AEI\/10.13039\/501100011033\/FEDER, UE","award":["PID2022-137278OB-I00"],"award-info":[{"award-number":["PID2022-137278OB-I00"]}]},{"name":"MCIN\/AEI\/10.13039\/501100011033\/FEDER, UE","award":["EVALKALIM II"],"award-info":[{"award-number":["EVALKALIM II"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>A miniaturized solid-phase extraction of two tropane alkaloids (TAs) and twenty-one pyrrolizidine alkaloids (PAs) from infusions of dry edible flowers using optimized \u00b5SPEed\u00ae technique was developed. The optimization of the \u00b5SPEed\u00ae methodology involved testing different cartridges and comparing various volumes and numbers of loading cycles. The final conditions allowed for a rapid extraction, taking only 3.5 min. This was achieved using a C18-ODS cartridge, conditioning with 100 \u00b5L of methanol (two cycles), loading 100 \u00b5L of the infusion sample (seven cycles), and eluting the analytes with 100 \u00b5L of methanol (two cycles). Prior to their analysis by UHPLC-IT-MS\/MS, the extracts were evaporated and reconstituted in 100 \u00b5L of water (0.2% formic acid)\/methanol (0.2% ammonia) 95:5 (v\/v), allowing for a preconcentration factor of seven times. The methodology was successfully validated obtaining recoveries ranging between 87 and 97%, RSD of less than 12%, and MQL between 0.09 and 0.2 \u00b5g\/L. The validated methodology was applied to twenty samples of edible flower infusions to evaluate the safety of these products. Two infusion samples obtained from Acmella oleracea and Viola tricolor were contaminated with 0.16 and 0.2 \u00b5g\/L of scopolamine (TA), respectively, while the infusion of Citrus aurantium was contaminated with intermedine and lycopsamine (PAs) below the MQL.<\/jats:p>","DOI":"10.3390\/foods13111740","type":"journal-article","created":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T03:51:45Z","timestamp":1717386705000},"page":"1740","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Simultaneous Determination of 23 Pyrrolizidine and Tropane Alkaloids in Infusions from Dry Edible Flowers Using Optimized \u03bcSPEed\u00ae Microextraction Prior to Their Analysis by UHPLC-IT-MS\/MS"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7109-8792","authenticated-orcid":false,"given":"Bego\u00f1a","family":"Fern\u00e1ndez-Pintor","sequence":"first","affiliation":[{"name":"Departamento de Tecnolog\u00eda Qu\u00edmica y Ambiental, Escuela Superior de Ciencias Experimentales y Tecnolog\u00eda, Universidad Rey Juan Carlos, C\/Tulip\u00e1n s\/n, M\u00f3stoles, 28933 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6448-2385","authenticated-orcid":false,"given":"Sonia","family":"Morante-Zarcero","sequence":"additional","affiliation":[{"name":"Departamento de Tecnolog\u00eda Qu\u00edmica y Ambiental, Escuela Superior de Ciencias Experimentales y Tecnolog\u00eda, Universidad Rey Juan Carlos, C\/Tulip\u00e1n s\/n, M\u00f3stoles, 28933 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2091-6772","authenticated-orcid":false,"given":"Isabel","family":"Sierra","sequence":"additional","affiliation":[{"name":"Departamento de Tecnolog\u00eda Qu\u00edmica y Ambiental, Escuela Superior de Ciencias Experimentales y Tecnolog\u00eda, Universidad Rey Juan Carlos, C\/Tulip\u00e1n s\/n, M\u00f3stoles, 28933 Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n de Tecnolog\u00edas para la Sostenibilidad, Universidad Rey Juan Carlos, C\/Tulip\u00e1n s\/n, M\u00f3stoles, 28933 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"569499","DOI":"10.3389\/fpls.2020.569499","article-title":"The Biodiversity of Edible Flowers: Discovering New Tastes and New Health Benefits","volume":"11","author":"Benvenutti","year":"2021","journal-title":"Front. 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