{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T04:06:03Z","timestamp":1778817963247,"version":"3.51.4"},"reference-count":51,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T00:00:00Z","timestamp":1717718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Finance Science and Technology Project of Hainan Province, China","award":["SQKY2022-0020"],"award-info":[{"award-number":["SQKY2022-0020"]}]},{"name":"The Finance Science and Technology Project of Hainan Province, China","award":["FW20230002"],"award-info":[{"award-number":["FW20230002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>The polysaccharides were extracted from the leaves of Mallotus oblongifolius (MO) using an ultrasonic-assisted extraction method in this study. The main variables affecting the yield of polysaccharides extracted from Mallotus appallatus (MOPS) were identified and optimized while concurrently investigating its antioxidant capacity, hypoglycemic activity, and digestive properties. The results indicated that the optimal ultrasound-assisted extraction of MOPS involved an ultrasound power of 200 W, a liquid-to-solid ratio of 25:1 (mL:g), an extraction temperature of 75 \u00b0C, and an ultrasound time of 45 min, leading to an extraction yield of (7.36 \u00b1 0.45)% (m\/m). The MOPS extract exhibited significant scavenging activity against DPPH and ABTS radicals with IC50 values of (25.65 \u00b1 0.53) \u03bcg\/mL and (100.38 \u00b1 0.38) \u03bcg\/mL, respectively. Furthermore, it effectively inhibited the enzymatic activities of \u03b1-glucosidase and \u03b1-amylase with IC50 values of (2.27 \u00b1 0.07) mg\/mL and (0.57 \u00b1 0.04) mg\/mL, respectively. The content of MOPS remained relatively stable in the stomach and small intestine; however, their ability to scavenge DPPH radicals and ABTS radicals and exhibit reducing power was attenuated, and the inhibition of \u03b1-amylase and \u03b1-glucosidase activity was diminished. In conclusion, the ultrasonic extraction of MOPS showed feasibility and revealed antioxidant and hypoglycemic effects. However, the activities were significantly reduced after gastric and small intestinal digestion despite no significant change in the MOPS content.<\/jats:p>","DOI":"10.3390\/foods13121799","type":"journal-article","created":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T10:43:42Z","timestamp":1717757022000},"page":"1799","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ultrasonic-Assisted Extraction and Gastrointestinal Digestion Characteristics of Polysaccharides Extracted from Mallotus oblongfolius"],"prefix":"10.3390","volume":"13","author":[{"given":"Gansheng","family":"Tan","sequence":"first","affiliation":[{"name":"Institute of Agro-Products Processing and Design, Hainan Academy of Agricultural Science, Haikou 571100, China"},{"name":"College of Food Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0752-8679","authenticated-orcid":false,"given":"Zhouwei","family":"Duan","sequence":"additional","affiliation":[{"name":"Institute of Agro-Products Processing and Design, Hainan Academy of Agricultural Science, Haikou 571100, China"},{"name":"Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7269-5751","authenticated-orcid":false,"given":"Guanghua","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian","family":"Xin","sequence":"additional","affiliation":[{"name":"Institute of Agro-Products Processing and Design, Hainan Academy of Agricultural Science, Haikou 571100, China"},{"name":"College of Food Science and Technology, Hainan University, Haikou 570228, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Agro-Products Processing and Design, Hainan Academy of Agricultural Science, Haikou 571100, China"},{"name":"Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Agro-Products Processing and Design, Hainan Academy of Agricultural Science, Haikou 571100, China"},{"name":"Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Agro-Products Processing and Design, Hainan Academy of Agricultural Science, Haikou 571100, China"},{"name":"Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1669","DOI":"10.1094\/PDIS-12-17-1957-PDN","article-title":"First Report of Colletotrichum siamense Causing Anthracnose on Partridge tea (Mallotus oblongifolius) in China","volume":"102","author":"Liu","year":"2018","journal-title":"Plant Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1039\/C9FO01790A","article-title":"Mallotus oblongifolius extracts ameliorate ischemic nerve damage by increasing endogenous neural stem cell proliferation through the Wnt\/beta-catenin signaling pathway","volume":"11","author":"Li","year":"2020","journal-title":"Food Funct."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"133004","DOI":"10.1016\/j.foodchem.2022.133004","article-title":"Screening and Identification of Natural A-Glucosidase and A-Amylase Inhibitors from Partridge Tea (Mallotus furetianus Muell-Arg) and in Silico Analysis","volume":"388","author":"Li","year":"2022","journal-title":"Food Chem."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Yu, S., Duan, Z., Li, P., Wang, S., Guo, L., Xia, G., and Xie, H. (2022). Protective Effect of Polyphenols Purified from Mallotus oblongfolius on Ethanol-Induced Gastric Mucosal Injury by Regulating Nrf2 and MAPKs Pathways. Antioxidants, 11.","DOI":"10.3390\/antiox11122452"},{"key":"ref_5","first-page":"6","article-title":"Survey and identification and evaluation of wild Mallotus oblongfolius resources in Hainan","volume":"11","author":"Gu","year":"2019","journal-title":"J. Trop. Crops"},{"key":"ref_6","first-page":"218","article-title":"Extraction and antioxidant properties of polyphenols from Mallotus oblongfolius","volume":"3","author":"Duan","year":"2020","journal-title":"Food Sci. Technol."},{"key":"ref_7","first-page":"847","article-title":"Comparison of antioxidant and antibacterial properties of polyphenols extracted from Mallotus oblongfolius by different methods","volume":"3","author":"Duan","year":"2021","journal-title":"J. Trop. Crops"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"948","DOI":"10.1016\/j.ijbiomac.2020.05.163","article-title":"Pre-Protective Effect of Polysaccharides Purified from Hericium Erinaceus against Ethanol-Induced Gastric Mucosal Injury in Rats","volume":"159","author":"Chen","year":"2020","journal-title":"Int. J. Biol. Macromo."},{"key":"ref_9","first-page":"31","article-title":"Effects of different extraction methods on the physicochemical properties and antioxidant effects of Poria coca polysaccharides","volume":"5","author":"Sun","year":"2021","journal-title":"J. Northwest Inst. Light Ind."},{"key":"ref_10","first-page":"184","article-title":"Separation and purification, structural characterisation and in vitro antioxidant properties of polysaccharides from Camellia sinensis","volume":"6","author":"Gong","year":"2021","journal-title":"Food Mach."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.29219\/fnr.v62.1599","article-title":"Oolong Tea Polysaccharide and Polyphenols Prevent Obesity Development in Sprague-Dawley Rats","volume":"62","author":"Wu","year":"2018","journal-title":"Food Nutr. Res."},{"key":"ref_12","first-page":"162","article-title":"Structural characteristics and hypoglycaemic activity of tea polysaccharides","volume":"19","author":"Song","year":"2018","journal-title":"Food Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1515\/ijfe-2020-0153","article-title":"Optimization of Ultrasonic Extraction of Lycium Barbarum Polysaccharides Using Response Surface Methodology","volume":"16","author":"Song","year":"2020","journal-title":"Int. J. Food Eng."},{"key":"ref_14","first-page":"215","article-title":"Optimisation of microwave extraction process of fig polysaccharide","volume":"12","author":"Li","year":"2021","journal-title":"Food Sci. Technol. Res."},{"key":"ref_15","first-page":"36","article-title":"Optimisation of polysaccharide extraction process of Alliumcepal.with Compound Enzyme Hot Water","volume":"12","author":"Chen","year":"2016","journal-title":"Asia-Pac. Tradit. Med."},{"key":"ref_16","first-page":"105","article-title":"Optimisation of ultrasonic-assisted extraction process of polysaccharides from summer and autumn tea","volume":"49","author":"Cao","year":"2023","journal-title":"Anhui Agric. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"100288","DOI":"10.1016\/j.fochx.2022.100288","article-title":"Dynamic Variations in Physicochemical Characteristics of Oolong Tea Polysaccharides During Simulated Digestion and Fecal Fermentation","volume":"14","author":"Wu","year":"2022","journal-title":"Food Chem. X"},{"key":"ref_18","first-page":"6","article-title":"Digestion and absorption of penicillin polysaccharides in an in vitro digestion model","volume":"21","author":"Min","year":"2013","journal-title":"Food Sci."},{"key":"ref_19","first-page":"78","article-title":"Optimization of Ultrasound-Assisted Extraction, Preliminary Characterization and in Vitro Antioxidant Activity of Polysaccharides from Green Pea Pods","volume":"4","author":"Mar","year":"2016","journal-title":"Foods"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1021\/ac60111a017","article-title":"Colorimetric Method for Determination of Sugars and Related Substances","volume":"28","author":"Dubois","year":"1956","journal-title":"Anal. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1016\/j.foodchem.2012.10.030","article-title":"Compositional Characterisation of Soluble Apple Polysaccharides, and Their Antioxidant and Hepatoprotective Effects on Acute Ccl4-Caused Liver Damage in Mice","volume":"138","author":"Yang","year":"2013","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.ijbiomac.2019.11.050","article-title":"Preparation, Deproteinization, Characterization, and Antioxidant Activity of Polysaccharide from Medemia Argun Fruit","volume":"155","author":"Mohammed","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1515\/ijfe-2019-0271","article-title":"Effect of microwave-drying on the quality and antioxidant properties of ganoderma lucidum fermented sea-buckthorn tea","volume":"17","author":"Xin","year":"2021","journal-title":"Int. J. Food Eng."},{"key":"ref_24","first-page":"7","article-title":"Response surface optimisation of alkaline degradation of polyproanthocyanidins from red raspberry seeds and inhibition of hypoglycaemic enzyme activity","volume":"4","author":"Ji","year":"2019","journal-title":"Sci. Technol. Food Ind."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"991","DOI":"10.1038\/s41596-018-0119-1","article-title":"Infogest Static in Vitro Simulation of Gastrointestinal Food Digestion","volume":"14","author":"Brodkorb","year":"2019","journal-title":"Nat. Protoc."},{"key":"ref_26","first-page":"99","article-title":"Optimisation of ultrasound-assisted extraction of polysaccharides from Silybum marianum","volume":"20","author":"Liu","year":"2012","journal-title":"Food Sci."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"755","DOI":"10.3136\/fstr.20.755","article-title":"Optimization of Microwave-Assisted Extraction of Water-Soluble Polysaccharides from Piteguo Fruit by Response Surface Methodology","volume":"20","author":"Wang","year":"2014","journal-title":"Food Sci. Technol. Res."},{"key":"ref_28","first-page":"101","article-title":"Response surface optimisation of seed melon polysaccharide extraction process and its in vitro hypolipidemic activity","volume":"2","author":"Qian","year":"2020","journal-title":"Sci. Technol. Food Ind."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1016\/j.foodhyd.2012.11.021","article-title":"Microwave-Assisted Extraction, Chemical Characterization of Polysaccharides from Lilium Davidii Var. Unicolor Salisb and Its Antioxidant Activities Evaluation","volume":"2","author":"Zhao","year":"2013","journal-title":"Food Hydrocoll."},{"key":"ref_30","first-page":"85","article-title":"Optimisation of alkaline extraction process of polysaccharides from the leaves of Tectona grandis by response surface methodology","volume":"9","author":"Zhang","year":"2023","journal-title":"Food Ind."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.procbio.2021.11.008","article-title":"Ultrasonic Enzyme-Assisted Extraction of Comfrey (Symphytum officinale L.) Polysaccharides and Their Digestion and Fermentation Behaviors in Vitro","volume":"112","author":"Cao","year":"2021","journal-title":"Process Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"117879","DOI":"10.1016\/j.carbpol.2021.117879","article-title":"Structural Characterization and Antioxidant Activities of One Neutral Polysaccharide and Three Acid Polysaccharides from Ziziphus Jujuba Cv. Hamidazao: A Comparison","volume":"261","author":"Yang","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_33","first-page":"66","article-title":"Studies on hypotensive, hypoglycaemic and antioxidant activities of noni leaf","volume":"19","author":"Zhang","year":"2013","journal-title":"Food Res. Dev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/j.carbpol.2016.04.001","article-title":"Purification, Structural Characterization and in Vitro Antioxidant Activity of a Novel Polysaccharide from Boshuzhi","volume":"147","author":"Jiang","year":"2016","journal-title":"Carbohyd. Polym."},{"key":"ref_35","first-page":"1973","article-title":"Study of the Protective Effect of Schizandra Chinensis Polysaccharide on Oxidative Damage of Mouse Spermatozoa in Vitro","volume":"914","author":"Zhao","year":"2014","journal-title":"Adv. Mater. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/j.ijbiomac.2010.06.010","article-title":"Structural Characterization of Polysaccharides from Zizyphus Jujuba and Evaluation of Antioxidant Activity","volume":"4","author":"Chang","year":"2010","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_37","first-page":"1113","article-title":"Modelling the Influence of Origin, Packing and Storage on Water Activity, Colour and Texture of Almonds, Hazelnuts and Walnuts Using Artificial Neural Networks","volume":"5","author":"Correia","year":"2015","journal-title":"Food Bioprocess Tech."},{"key":"ref_38","first-page":"859","article-title":"In vitro simulated digestion of polysaccharides from Agaricus bisporus","volume":"6","author":"Jin","year":"2023","journal-title":"J. Hefei Univ. Technol. Nat. Sci."},{"key":"ref_39","first-page":"269","article-title":"Progress of extraction, purification, structural characterisation and bioactivity of polysaccharides from Auricularia auricula","volume":"8","author":"Yin","year":"2022","journal-title":"Food Ind."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"952","DOI":"10.1016\/j.foodhyd.2018.09.014","article-title":"Effects of in Vitro Saliva, Gastric and Intestinal Digestion on the Chemical Properties, Antioxidant Activity of Polysaccharide from Artocarpus heterophyllus Lam. (Jackfruit) Pulp","volume":"87","author":"Zhu","year":"2019","journal-title":"Food Hydrocoll."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sharma, B.R., and Dong, Y.R. (2014). Anti-Diabetic Effects of Caulerpa Lentillifera: Stimulation of Insulin Secretion in Pancreatic \u0392-Cells and Enhancement of Glucose Uptake in Adipocytes. Asian Pac. J. Trop. Biomed., 7.","DOI":"10.12980\/APJTB.4.2014APJTB-2014-0091"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.foodchem.2007.06.040","article-title":"Antioxidant Activities of Different Fractions of Polysaccharide Conjugates from Green Tea (Camellia sinensis)","volume":"2","author":"Chen","year":"2008","journal-title":"Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/j.foodres.2017.10.058","article-title":"Effects of Simulated Gastrointestinal Digestion in Vitro on the Chemical Properties, Antioxidant Activity, A-Amylase and A-Glucosidase Inhibitory Activity of Polysaccharides from Inonotus Obliquus","volume":"103","author":"Wang","year":"2018","journal-title":"Food Res. Int."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.foodhyd.2018.11.040","article-title":"Effect of simulated gastrointestinal digestion in vitro on the antioxidant activity, molecular weight and microstructure of polysaccharides from a tropical sea cucumber (holothuria leucospilota)","volume":"89","author":"Yuan","year":"2019","journal-title":"Food Hydrocoll."},{"key":"ref_45","first-page":"7","article-title":"Changes in antioxidant activity of polysaccharides from grapefruit peel in vitro digestion","volume":"11","author":"Wan","year":"2021","journal-title":"Food Res. Dev."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1002\/fsn3.2176","article-title":"Hypoglycemic effect of Taraxacum officinale root extract and its synergism with Radix Astragali extract","volume":"4","author":"Li","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_47","first-page":"3292","article-title":"Effect of different drying methods on the bioactive compounds and bioactivities of walnut green peel","volume":"9","author":"Du","year":"2018","journal-title":"J. Food Saf. Qual."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0144-8617(00)00294-0","article-title":"Structural Characterization of Intestinal Immune System Modulating New Arabino-3, 6-Galactan from Rhizomes of Atractylodes Lancea Dc","volume":"46","author":"Yu","year":"2001","journal-title":"Carbohydr. Polym."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"C469","DOI":"10.1111\/j.1750-3841.2009.01231.x","article-title":"Physicochemical Properties and Antioxidant Capacity of 3 Polysaccharides from Green Tea, Oolong Tea, and Black Tea","volume":"74","author":"Chen","year":"2009","journal-title":"J. Food Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1016\/j.ijbiomac.2016.10.102","article-title":"In Vitro Antioxidative and Immunological Activities of Polysaccharides from Zizyphus Jujuba Cv. Muzao","volume":"95","author":"Zhang","year":"2017","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_51","first-page":"1592","article-title":"Isolation and purification of polysaccharide from Moringa oleifera leaf and its hypoglycemic activity in vitro","volume":"9","author":"Wang","year":"2018","journal-title":"J. Food Saf. Qual."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/13\/12\/1799\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:55:27Z","timestamp":1760108127000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/13\/12\/1799"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,7]]},"references-count":51,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["foods13121799"],"URL":"https:\/\/doi.org\/10.3390\/foods13121799","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,7]]}}}