{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T01:31:22Z","timestamp":1794447082902,"version":"4.9.1"},"reference-count":27,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T00:00:00Z","timestamp":1734480000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014440","name":"MICIN\/AEI\/10.13039\/501100011033","doi-asserted-by":"publisher","award":["PID2020-117670RB-C21"],"award-info":[{"award-number":["PID2020-117670RB-C21"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Edible legume sprouts have been proposed as a promising plant-based source of the enzyme diamine oxidase (DAO), which plays a key role in degrading histamine at an intestinal level and preventing the development of histamine intolerance symptoms. However, the temperature and humidity conditions required for seed germination can also favor the rapid growth of yeast and mold, potentially compromising sprout yield and quality. The aim of this study was to evaluate the influence of different seed disinfection treatments on both the germination rate and DAO enzymatic activity in sprouts of four Leguminosae species. Seed disinfection with 70% ethanol for either 5 or 15 min slightly increased the germination rates of chickpea and soybean sprouts without affecting DAO activity, regardless of treatment duration. However, in lentil and green pea sprouts, ethanol disinfection caused a statistically significant reduction in histamine-degrading capacity. In contrast, treating seeds with sodium hypochlorite for 15 min increased germination rates by up to 14% and preserved DAO activity in all legume sprouts tested. These results indicate that incorporating a seed disinfection step during legume sprouting may affect both the DAO enzymatic activity and germination rate.<\/jats:p>","DOI":"10.3390\/foods13244105","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T11:17:22Z","timestamp":1734520642000},"page":"4105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Influence of Seed Disinfection Treatments on the Germination Rate and Histamine-Degrading Activity of Legume Sprouts"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0613-9244","authenticated-orcid":false,"given":"Judit","family":"Costa-Catala","sequence":"first","affiliation":[{"name":"Departament de Nutrici\u00f3, Ci\u00e8ncies de l\u2019Alimentaci\u00f3 i Gastronomia, Campus de l\u2019Alimentaci\u00f3 de Torribera, Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"},{"name":"Institut de Recerca en Nutrici\u00f3 i Seguretat Aliment\u00e0ria (INSA\u00b7UB), Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8850-1026","authenticated-orcid":false,"given":"Jaume","family":"Bori","sequence":"additional","affiliation":[{"name":"Associated British Foods Ingredients (ABFI), Escoles P\u00edes 49, 08017 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8633-1860","authenticated-orcid":false,"given":"M. Teresa","family":"Veciana-Nogu\u00e9s","sequence":"additional","affiliation":[{"name":"Departament de Nutrici\u00f3, Ci\u00e8ncies de l\u2019Alimentaci\u00f3 i Gastronomia, Campus de l\u2019Alimentaci\u00f3 de Torribera, Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"},{"name":"Institut de Recerca en Nutrici\u00f3 i Seguretat Aliment\u00e0ria (INSA\u00b7UB), Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8215-7180","authenticated-orcid":false,"given":"M. Luz","family":"Latorre-Moratalla","sequence":"additional","affiliation":[{"name":"Departament de Nutrici\u00f3, Ci\u00e8ncies de l\u2019Alimentaci\u00f3 i Gastronomia, Campus de l\u2019Alimentaci\u00f3 de Torribera, Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"},{"name":"Institut de Recerca en Nutrici\u00f3 i Seguretat Aliment\u00e0ria (INSA\u00b7UB), Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Carmen","family":"Vidal-Carou","sequence":"additional","affiliation":[{"name":"Departament de Nutrici\u00f3, Ci\u00e8ncies de l\u2019Alimentaci\u00f3 i Gastronomia, Campus de l\u2019Alimentaci\u00f3 de Torribera, Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"},{"name":"Institut de Recerca en Nutrici\u00f3 i Seguretat Aliment\u00e0ria (INSA\u00b7UB), Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9411-0395","authenticated-orcid":false,"given":"Oriol","family":"Comas-Bast\u00e9","sequence":"additional","affiliation":[{"name":"Departament de Nutrici\u00f3, Ci\u00e8ncies de l\u2019Alimentaci\u00f3 i Gastronomia, Campus de l\u2019Alimentaci\u00f3 de Torribera, Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"},{"name":"Institut de Recerca en Nutrici\u00f3 i Seguretat Aliment\u00e0ria (INSA\u00b7UB), Universitat de Barcelona, Av. Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Chomdom Kounga, P., Tchoumi Neree, A., Pietrangeli, P., Marcocci, L., and Mateescu, M.A. (2022). Faster and Sensitive Zymographic Detection of Oxidases Generating Hydrogen Peroxide. The Case of Diamine Oxidase. Anal. Biochem., 648.","DOI":"10.1016\/j.ab.2022.114676"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Strnad, J., Soural, M., and \u0160ebela, M. (2024). A New Activity Assay Method for Diamine Oxidase Based on Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry. 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