{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:57:55Z","timestamp":1792807075229,"version":"4.5.0"},"reference-count":53,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T00:00:00Z","timestamp":1734652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2022YFD2101003"],"award-info":[{"award-number":["2022YFD2101003"]}]},{"name":"National Key R&amp;D Program of China","award":["2024SFQZX014"],"award-info":[{"award-number":["2024SFQZX014"]}]},{"name":"National Key R&amp;D Program of China","award":["B18053"],"award-info":[{"award-number":["B18053"]}]},{"name":"National Innovation Demonstration Zone (Zaozhuang) for Sustainable Development of China","award":["2022YFD2101003"],"award-info":[{"award-number":["2022YFD2101003"]}]},{"name":"National Innovation Demonstration Zone (Zaozhuang) for Sustainable Development of China","award":["2024SFQZX014"],"award-info":[{"award-number":["2024SFQZX014"]}]},{"name":"National Innovation Demonstration Zone (Zaozhuang) for Sustainable Development of China","award":["B18053"],"award-info":[{"award-number":["B18053"]}]},{"name":"111 project from the Education Ministry of China","award":["2022YFD2101003"],"award-info":[{"award-number":["2022YFD2101003"]}]},{"name":"111 project from the Education Ministry of China","award":["2024SFQZX014"],"award-info":[{"award-number":["2024SFQZX014"]}]},{"name":"111 project from the Education Ministry of China","award":["B18053"],"award-info":[{"award-number":["B18053"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Chicken yolk immunoglobulin (IgY) is a natural immunologically active antibody extracted from egg yolk and can be used as a natural dietary supplement for the treatment of inflammation and damage to the intestines. In our study, IgY was embedded in a double emulsion (W\/O\/W; DE) to explore the therapeutic effect of the embedded IgY on Lipopolysaccharide (LPS)-induced jejunal injury in mice. The results showed that W\/O\/W-embedded IgY as a dietary supplement (IgY + DE) attenuated LPS-induced damage to mouse small intestinal structures and protected the integrity of the jejunal mucosal barrier. IgY + DE increased the amount of related transcription factors (Math1, Spdef, Elf3, and Klf4) and promoted thrush cell differentiation. IgY + DE ameliorated LPS-induced reduction in mucin quantity and markers. It promoted the expression of Muc1 and Muc2 and increased the mRNA expression levels of Muc1, Muc2, Muc3, Muc4, Muc13, and Agr2 (p &lt; 0.05). IgY + DE increased the expression of several glycosyltransferases involved in mucin glycosylation. IgY + DE also neutralized the LPS attack on the expression of jejunal inflammatory factors IL-1\u03b2, IL-6, IL-4, and TNF-\u03b1. In conclusion, the IgY-embedded double emulsion can be used as a dietary supplement for immunotherapy to prevent LPS-induced jejunal injury in mice.<\/jats:p>","DOI":"10.3390\/foods13244138","type":"journal-article","created":{"date-parts":[[2024,12,23]],"date-time":"2024-12-23T09:13:38Z","timestamp":1734945218000},"page":"4138","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Amelioration of LPS-Induced Jejunum Injury and Mucus Barrier Damage in Mice by IgY Embedded in W\/O\/W Emulsion"],"prefix":"10.3390","volume":"13","author":[{"given":"Zhaohui","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihua","family":"Ye","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shidi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3502-7432","authenticated-orcid":false,"given":"Chuanming","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kongdi","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9975-6737","authenticated-orcid":false,"given":"Pengjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Tibet Tianhong Science and Technology Co., Ltd., Lasha 851414, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6501-3086","authenticated-orcid":false,"given":"Jiaqiang","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, Ministry of Education, China Agricultural University, Beijing 100083, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.jcmgh.2017.11.001","article-title":"The endosomal protein endotubin is required for enterocyte differentiation","volume":"5","author":"Cox","year":"2018","journal-title":"Cell Mol. 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