{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T10:27:57Z","timestamp":1783247277663,"version":"3.54.6"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T00:00:00Z","timestamp":1736899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Council of Agriculture in Taiwan","award":["111AS-11.1.2-CI-C1"],"award-info":[{"award-number":["111AS-11.1.2-CI-C1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Vitamin D, essential for growth and health, is often deficient in Taiwan despite abundant sunlight. Plant-derived vitamin D2 (ergocalciferol) is bioavailable, environmentally friendly, and cost-effective. This study evaluated the efficacy of enhancing Pleurotus citrinopileatus (PC) mushrooms\u2019 vitamin D2 content through pulsed ultraviolet (PUV) light and its impact on vitamin D status in humans. In a four-week randomized parallel trial, 36 healthy participants were assigned to three groups: a control group, a group consuming 10 g\/day PUV-treated PC (PC-10 g), and a group consuming 100 g\/day PUV-treated PC (PC-100 g). Blood samples collected pre- and post-intervention measured serum 25(OH)D2, 25(OH)D3, and biochemical parameters. After four weeks, serum 25(OH)D2 levels significantly increased in the PC-10 g group (1.47 \u00b1 1.42 ng\/mL to 9.50 \u00b1 7.10 ng\/mL, p = 0.001) and in the PC-100 g group (1.94 \u00b1 2.15 ng\/mL to 21.82 \u00b1 16.75 ng\/mL, p = 0.002), showing a 10.2-fold rise. The PC-100 g group also experienced a 37.6% reduction in serum intact parathyroid hormone (I-PTH) levels (26.26 \u00b1 9.84 pg\/mL to 16.38 \u00b1 5.53 pg\/mL). No adverse effects were reported. PUV-treated PC mushrooms significantly increase serum 25(OH)D2 levels and reduce I-PTH, particularly at higher doses. These findings underscore the potential of vitamin-D-enriched PC as a sustainable, fungi-derived food source for addressing vitamin D deficiency.<\/jats:p>","DOI":"10.3390\/foods14020259","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T05:59:42Z","timestamp":1736920782000},"page":"259","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Effects of Consuming Pulsed UV Light-Treated Pleurotus citrinopileatus on Vitamin D Nutritional Status in Healthy Adults"],"prefix":"10.3390","volume":"14","author":[{"given":"Chih-Ching","family":"Hsu","sequence":"first","affiliation":[{"name":"School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0706-750X","authenticated-orcid":false,"given":"Chiao-Ming","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei 10462, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ming","family":"Ju","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ching","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huei-Mei","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Hui","family":"Yang","sequence":"additional","affiliation":[{"name":"Taiwan Agricultural Research Institute, Fengshan Tropical Horticultural Experiment Branch, Kaohsiung City 83052, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9060-709X","authenticated-orcid":false,"given":"Chien-Tien","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Family Medicine, Taipei Medical University Hospital, Taipei 11031, Taiwan"},{"name":"School of Public Health, College of Public Health, Taipei Medical University, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5577-9280","authenticated-orcid":false,"given":"Te-Chao","family":"Fang","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"Division of Nephrology, Department of Internal Medicine, Taipei Medical University Hospital, Taipei Medical University, Taipei 11031, Taiwan"},{"name":"Taipei Medical University-Research Center of Urology and Kidney, Taipei Medical University, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4316-8888","authenticated-orcid":false,"given":"Widiastuti","family":"Setyaningsih","sequence":"additional","affiliation":[{"name":"Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jalan Flora, Depok, Sleman, Yogyakarta 55281, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4244-7911","authenticated-orcid":false,"given":"Sing-Chung","family":"Li","sequence":"additional","affiliation":[{"name":"School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei 11031, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wimalawansa, S.J. (2024). Physiology of Vitamin D\u2014Focusing on Disease Prevention. Nutrients, 16.","DOI":"10.3390\/nu16111666"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1152\/physrev.00014.2015","article-title":"Vitamin D: Metabolism, molecular mechanism of action, and pleiotropic effects","volume":"96","author":"Christakos","year":"2016","journal-title":"Physiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.chembiol.2013.12.016","article-title":"Vitamin D metabolism, mechanism of action, and clinical applications","volume":"21","author":"Bikle","year":"2014","journal-title":"Chem. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lee, M.J., Hsu, H.J., Wu, I.W., Sun, C.Y., Ting, M.K., and Lee, C.C. (2019). Vitamin D deficiency in northern Taiwan: A community-based cohort study. BMC Public Health, 19.","DOI":"10.1186\/s12889-019-6657-9"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1016\/S0002-8223(00)00134-6","article-title":"Dietary intake of vitamin D in premenopausal, healthy vegans was insufficient to maintain concentrations of serum 25-hydroxyvitamin D and intact parathyroid hormone within normal ranges during the winter in Finland","volume":"100","author":"Outila","year":"2000","journal-title":"J. Am. Diet. Assoc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1686s","DOI":"10.3945\/ajcn.2009.26736X","article-title":"Serum 25-hydroxyvitamin D status of vegetarians, partial vegetarians, and nonvegetarians: The Adventist Health Study-2","volume":"89","author":"Chan","year":"2009","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1016\/j.jfda.2018.12.010","article-title":"Using gas chromatography and mass spectrometry to determine 25-hydroxyvitamin D levels for clinical assessment of vitamin D deficiency","volume":"27","author":"Yang","year":"2019","journal-title":"J. Food Drug Anal."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"165","DOI":"10.4161\/derm.23321","article-title":"Photobiology of vitamin D in mushrooms and its bioavailability in humans","volume":"5","author":"Keegan","year":"2013","journal-title":"Dermato-Endocrinology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7841","DOI":"10.1021\/jf104246z","article-title":"Vitamin D and sterol composition of 10 types of mushrooms from retail suppliers in the United States","volume":"59","author":"Phillips","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1021\/jf203825e","article-title":"Effects of postharvest pulsed UV light treatment of white button mushrooms (Agaricus bisporus) on vitamin D2 content and quality attributes","volume":"60","author":"Kalaras","year":"2012","journal-title":"J. Agric. Food Chem."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Cardwell, G., Bornman, J.F., James, A.P., and Black, L.J. (2018). A review of mushrooms as a potential source of dietary vitamin D. Nutrients, 10.","DOI":"10.3390\/nu10101498"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Hao, Y., Sun, H., Zhang, X., Wu, L., and Zhu, Z. (2020). A novel polysaccharide from Pleurotus citrinopileatus mycelia: Structural characterization, hypoglycemic activity and mechanism. Food Biosci., 37.","DOI":"10.1016\/j.fbio.2020.100735"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/12298093.2020.1835142","article-title":"Cultivation and nutritional value of prominent Pleurotus spp.: An overview","volume":"49","author":"Raman","year":"2021","journal-title":"Mycobiology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.jfca.2015.02.005","article-title":"Vitamin D2 content and antioxidant properties of fruit body and mycelia of edible mushrooms by UV-B irradiation","volume":"42","author":"Huang","year":"2015","journal-title":"J. Food Compos. Anal."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.24294\/ace.v7i1.2312","article-title":"Enhancement of nutraceutical components of mushroom by uv exposure and extension of their shelf-life using edible coating material adopting online assessment of keeping quality by Magnetic Resonance Imaging (MRI) technique","volume":"7","author":"Tidke","year":"2024","journal-title":"Appl. Chem. Eng."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Rathore, H., Prasad, S., Sehwag, S., and Sharma, S. (2020). Vitamin D 2 fortification of Calocybe indica mushroom by natural and artificial UVB radiations and their potential effects on nutraceutical properties. 3 Biotech, 10.","DOI":"10.1007\/s13205-019-2024-x"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Judprasong, K., Chheng, S., Chimkerd, C., Jittinandana, S., Tangsuphoom, N., and Sridonpai, P. (2023). Effect of Ultraviolet Irradiation on Vitamin D in Commonly Consumed Mushrooms in Thailand. Foods, 12.","DOI":"10.3390\/foods12193632"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1016\/j.jfca.2011.02.007","article-title":"Concentration of vitamin D2 in white button mushrooms (Agaricus bisporus) exposed to pulsed UV light","volume":"24","author":"Koyyalamudi","year":"2011","journal-title":"J. Food Compos. Anal."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Chen, C.M., Mu, S.C., Chen, Y.L., Tsai, L.Y., Kuo, Y.T., Cheong, I.M., Chang, M.L., and Li, S.C. (2020). Infants\u2019 Vitamin D Nutritional Status in the First Year of Life in Northern Taiwan. Nutrients, 12.","DOI":"10.3390\/nu12020404"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1038\/s41574-023-00942-0","article-title":"A view on vitamin D: A pleiotropic factor?","volume":"20","author":"Giustina","year":"2024","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Cui, A., Zhang, T., Xiao, P., Fan, Z., Wang, H., and Zhuang, Y. (2023). Global and regional prevalence of vitamin D deficiency in population-based studies from 2000 to 2022: A pooled analysis of 7.9 million participants. Front. Nutr., 10.","DOI":"10.3389\/fnut.2023.1070808"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"451","DOI":"10.4162\/nrp.2023.17.3.451","article-title":"Estimated dietary vitamin D intake and major vitamin D food sources of Koreans: Based on the Korea National Health and Nutrition Examination Survey 2016\u20132019","volume":"17","author":"Kim","year":"2023","journal-title":"Nutr. Res. Pract."},{"key":"ref_23","first-page":"397","article-title":"Vitamin D intake and its food sources in Taiwanese","volume":"17","author":"Lee","year":"2008","journal-title":"Asia Pac. J. Clin. Nutr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.medcli.2019.03.019","article-title":"An epidemiology survey of vitamin D deficiency and its influencing factors","volume":"154","author":"Jiang","year":"2020","journal-title":"Med. Clin."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2739","DOI":"10.1021\/acsfoodscitech.4c00701","article-title":"Escalating Vitamin D2 Content in Fresh Sporocarps of Elm Oyster Mushroom Hypsizygus ulmarius (Bull.) Redhead (Agaricomycetes) Using Ultraviolet Irradiation and Assessment of Its Stability, Antioxidant Capacity, Color, and Textural Properties","volume":"4","author":"Aditya","year":"2024","journal-title":"ACS Food Sci. Technol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.foodchem.2012.04.132","article-title":"Generation of potentially bioactive ergosterol-derived products following pulsed ultraviolet light exposure of mushrooms (Agaricus bisporus)","volume":"135","author":"Kalaras","year":"2012","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1038\/ejcn.2011.53","article-title":"Bioavailability of vitamin D\u2082 from UV-B-irradiated button mushrooms in healthy adults deficient in serum 25-hydroxyvitamin D: A randomized controlled trial","volume":"65","author":"Urbain","year":"2011","journal-title":"Eur. J. Clin. Nutr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.3945\/jn.112.159764","article-title":"Ergocalciferol from mushrooms or supplements consumed with a standard meal increases 25-hydroxyergocalciferol but decreases 25-hydroxycholecalciferol in the serum of healthy adults","volume":"142","author":"Stephensen","year":"2012","journal-title":"J. Nutr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.3945\/ajcn.2009.27972","article-title":"Fortification of orange juice with vitamin D(2) or vitamin D(3) is as effective as an oral supplement in maintaining vitamin D status in adults","volume":"91","author":"Biancuzzo","year":"2010","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1210\/jc.2007-2308","article-title":"Vitamin D2 is as effective as vitamin D3 in maintaining circulating concentrations of 25-hydroxyvitamin D","volume":"93","author":"Holick","year":"2008","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Kleftaki, S.-A., Amerikanou, C., Gioxari, A., Lantzouraki, D.Z., Sotiroudis, G., Tsiantas, K., Tsiaka, T., Tagkouli, D., Tzavara, C., and Lachouvaris, L. (2022). A Randomized Controlled Trial on Pleurotus eryngii Mushrooms with Antioxidant Compounds and Vitamin D2 in Managing Metabolic Disorders. Antioxidants, 11.","DOI":"10.3390\/antiox11112113"},{"key":"ref_32","first-page":"e9764","article-title":"Response of Parathyroid Hormone to Vitamin D Deficiency in Otherwise Healthy Individuals","volume":"12","author":"Malik","year":"2020","journal-title":"Cureus"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Malik, M.A., Jan, Y., Al-Keridis, L.A., Haq, A., Ahmad, J., Adnan, M., Alshammari, N., Ashraf, S.A., and Panda, B.P. (2022). Effect of Vitamin-D-Enriched Edible Mushrooms on Vitamin D Status, Bone Health and Expression of CYP2R1, CYP27B1 and VDR Gene in Wistar Rats. J. Fungi, 8.","DOI":"10.3390\/jof8080864"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1080\/09168451.2019.1576497","article-title":"Effects of vitamin D(2)-fortified shiitake mushroom on bioavailability and bone structure","volume":"83","author":"Won","year":"2019","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1002\/fsn3.2120","article-title":"Nutritional impact of adding a serving of mushrooms on usual intakes and nutrient adequacy using National Health and Nutrition Examination Survey 2011\u20132016 data","volume":"9","author":"Fulgoni","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Starck, C., Cassettari, T., Wright, J., Petocz, P., Beckett, E., and Fayet-Moore, F. (2024). Mushrooms: A food-based solution to vitamin D deficiency to include in dietary guidelines. Front. Nutr., 11.","DOI":"10.3389\/fnut.2024.1384273"},{"key":"ref_37","first-page":"e8817","article-title":"Safety of vitamin D(2) mushroom powder as a Novel food pursuant to Regulation (EU) 2015\/2283 (NF 2020\/2226)","volume":"22","author":"Turck","year":"2024","journal-title":"EFSA J."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/14\/2\/259\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:29:06Z","timestamp":1759919346000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/14\/2\/259"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,15]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["foods14020259"],"URL":"https:\/\/doi.org\/10.3390\/foods14020259","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,15]]}}}