{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T13:02:16Z","timestamp":1793710936083,"version":"4.6.1"},"reference-count":87,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,2,8]],"date-time":"2025-02-08T00:00:00Z","timestamp":1738972800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Moldovan Government","award":["24.80012.5107.06TC"],"award-info":[{"award-number":["24.80012.5107.06TC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>This study explores the production and valorization of hemp seed cake protein concentrate (HPC) as a functional ingredient to enhance the nutritional quality and sensory attributes of choux pastry products, specifically \u00e9clairs. By integrating varied concentrations of HPC (0%, 1%, 5%, 10%, 15%, and 20%) into traditional formulations, the physicochemical properties, proximate composition, amino acid profile, and sensory characteristics of the resulting pastries were assessed. Sensory attributes were assessed using the check-all-that-apply (CATA) method, where a trained panel selected applicable descriptors from a predefined list. Results indicated that the incorporation of HPC significantly increased protein content from 8.23% in the control sample (HPC0%) to 11.32% in the HPC20% formulation and improved moisture retention, leading to greater exterior and interior \u00e9clairs volume, increasing from 42.15 cm3 to 51.5 cm3 and from 18.34 cm3 to 38.47 cm3, respectively. Furthermore, sensory evaluation revealed pronounced differences in attributes such as flavor, appearance, and mouthfeel, with optimal sensory profiles noted at 10% HPC inclusion. The amino acid analysis demonstrated a balanced composition, particularly of essential amino acids, emphasizing HPC\u2019s potential as a valuable protein source, with significant contributions from leucine (8.17 g\/100 g protein), isoleucine (5.56 g\/100 g protein), and phenylalanine (6.31 g\/100 g protein), as well as notable levels of immunoactive amino acids such as arginine (10.92 g\/100 g protein) and glutamic acid (20.16 g\/100 g protein). These findings highlight the significant nutritional benefits of HPC enrichment, supporting the development of healthier bakery products and contributing to sustainable food practices within the industry.<\/jats:p>","DOI":"10.3390\/foods14040567","type":"journal-article","created":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T04:37:04Z","timestamp":1739335024000},"page":"567","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Harnessing Hemp (Cannabis sativa L.) Seed Cake Proteins: From Concentrate Production to Enhanced Choux Pastry Quality"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0056-5939","authenticated-orcid":false,"given":"Tatiana","family":"Capcanari","sequence":"first","affiliation":[{"name":"Department of Food and Nutrition, Technical University of Moldova, 9\/9 Studentilor St., MD-2045 Chisinau, Moldova"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4574-2959","authenticated-orcid":false,"given":"Eugenia","family":"Covaliov","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Technical University of Moldova, 9\/9 Studentilor St., MD-2045 Chisinau, Moldova"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u0103t\u0103lina","family":"Negoi\u021ba","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Technical University of Moldova, 9\/9 Studentilor St., MD-2045 Chisinau, Moldova"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Abbattista, R., Ventura, G., Calvano, C.D., Cataldi, T.R.I., and Losito, I. (2021). Bioactive Compounds in Waste By-Products from Olive Oil Production: Applications and Structural Characterization by Mass Spectrometry Techniques. Foods, 10.","DOI":"10.3390\/foods10061236"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"109683","DOI":"10.1016\/j.foodres.2020.109683","article-title":"Valorization of By-Products from Olive Oil Industry and Added-Value Applications for Innovative Functional Foods","volume":"137","author":"Astray","year":"2020","journal-title":"Food Res. Int."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ancu\u021ba, P., and Sonia, A. (2020). Oil Press-Cakes and Meals Valorization through Circular Economy Approaches: A Review. Appl. Sci., 10.","DOI":"10.3390\/app10217432"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"52","DOI":"10.21323\/2618-9771-2024-7-1-52-58","article-title":"Hemp (Cannabis sativa L.) Seeds Nutritional Aspects and Food Production Perspectives: A Review","volume":"7","author":"Capcanari","year":"2024","journal-title":"Food Syst."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.tifs.2021.01.014","article-title":"Biorefining of Seed Oil Cakes as Industrial Co-Streams for Production of Innovative Bioplastics. A Review","volume":"109","author":"Mirpoor","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Thakur, M., Modi, V.K., Khedkar, R., and Singh, K. (2020). Waste from Oil-Seed Industry: A Sustainable Approach. Sustainable Food Waste Management: Concepts and Innovations, Springer.","DOI":"10.1007\/978-981-15-8967-6"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.1111\/1750-3841.15719","article-title":"Sunflower Seed Byproduct and Its Fractions for Food Application: An Attempt to Improve the Sustainability of the Oil Process","volume":"86","author":"Egea","year":"2021","journal-title":"J. Food Sci."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Rakita, S., Koki\u0107, B., Manoni, M., Mazzoleni, S., Lin, P., Luciano, A., Ottoboni, M., Cheli, F., and Pinotti, L. (2023). Cold-Pressed Oilseed Cakes as Alternative and Sustainable Feed Ingredients: A Review. Foods, 12.","DOI":"10.3390\/foods12030432"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2599","DOI":"10.1002\/fsn3.3322","article-title":"Innovative Applications and Therapeutic Potential of Oilseeds and Their By-Products: An Eco-Friendly and Sustainable Approach","volume":"11","author":"Usman","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Svanes, E., Waalen, W., and Uhlen, A.K. (2020). Environmental Impacts of Rapeseed and Turnip Rapeseed Grown in Norway, Rape Oil and Press Cake. Sustainability, 12.","DOI":"10.3390\/su122410407"},{"key":"ref_11","first-page":"1","article-title":"The Use of Oilseed Wastes in The Food Industry","volume":"4","author":"Esmer","year":"2024","journal-title":"Int. J. Curr. Nat. Adv. Phytochem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.puhe.2023.10.003","article-title":"Health Inequalities of Global Protein-Energy Malnutrition from 1990 to 2019 and Forecast Prevalence for 2044: Data from the Global Burden of Disease Study 2019","volume":"225","author":"Jiang","year":"2023","journal-title":"Public Health"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Xiao, L., Fu, J., Lin, L., and Han, Y. (2022). Temporal Trends in Incidence of Nutritional Deficiency among Older Adults in China: A Secondary Analysis of the Global Burden of Disease Study 1990\u20132019. Nutrients, 14.","DOI":"10.3390\/nu14235008"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/s41580-020-0250-z","article-title":"Mechanisms, Regulation and Functions of the Unfolded Protein Response","volume":"21","author":"Hetz","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e161","DOI":"10.1002\/mco2.161","article-title":"Heat Shock Proteins: Biological Functions, Pathological Roles, and Therapeutic Opportunities","volume":"3","author":"Hu","year":"2022","journal-title":"MedComm"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1038\/s41580-020-00294-x","article-title":"Global View of Human Protein Glycosylation Pathways and Functions","volume":"21","author":"Schjoldager","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Mann, J., Truswell, A.S., and Hodson, L. (2023). Essentials of Human Nutrition, Oxford University Press.","DOI":"10.1093\/hesc\/9780198866671.001.0001"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Geissler, C., and Powers, H.J. (2023). Human Nutrition, Oxford University Press.","DOI":"10.1093\/hesc\/9780198866657.001.0001"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2","DOI":"10.4103\/ijpvm.IJPVM_48_19","article-title":"Trace Elements in Human Nutrition (II)\u2014An Update","volume":"11","author":"Mehri","year":"2020","journal-title":"Int. J. Prev. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.tifs.2020.03.044","article-title":"Oilseeds beyond Oil: Press Cakes and Meals Supplying Global Protein Requirements","volume":"100","author":"Arrutia","year":"2020","journal-title":"Trends Food Sci. Technol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"B\u00e1rta, J., B\u00e1rtov\u00e1, V., Jaro\u0161ov\u00e1, M., \u0160vajner, J., Smetana, P., Kadlec, J., Filip, V., Kyselka, J., Ber\u010d\u00edkov\u00e1, M., and Zdr\u00e1hal, Z. (2021). Oilseed Cake Flour Composition, Functional Properties and Antioxidant Potential as Effects of Sieving and Species Differences. Foods, 10.","DOI":"10.3390\/foods10112766"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Capcanari, T., Covaliov, E., Negoi\u021ba, C., Siminiuc, R., Chirsanova, A., Re\u0219itca, V., and \u021aurcanu, D. (2023). Hemp Seed Cake Flour as a Source of Proteins, Minerals and Polyphenols and Its Impact on the Nutritional, Sensorial and Technological Quality of Bread. Foods, 12.","DOI":"10.3390\/foods12234327"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.tifs.2020.10.004","article-title":"Oilseed Proteins\u2014Properties and Application as a Food Ingredient","volume":"106","author":"Sumara","year":"2020","journal-title":"Trends Food Sci. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Gil, M., Rudy, M., Duma-Kocan, P., Stanis\u0142awczyk, R., Krajewska, A., Dziki, D., and Hassoon, W.H. (2024). Sustainability of Alternatives to Animal Protein Sources, a Comprehensive Review. Sustainability, 16.","DOI":"10.3390\/su16177701"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"188","DOI":"10.21323\/2618-9771-2024-7-2-188-197","article-title":"Production of plant protein from seeds and cake of industrial hemp: Overview of processing methods for food industry","volume":"7","author":"Aleksanochkin","year":"2024","journal-title":"Food Syst."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"100779","DOI":"10.1016\/j.fpsl.2021.100779","article-title":"Hemp (Cannabis sativa) Seed Oilcake as a Promising by-Product for Developing Protein-Based Films: Effect of Transglutaminase-Induced Crosslinking","volume":"31","author":"Mirpoor","year":"2022","journal-title":"Food Packag. Shelf Life"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Crini, G., and Lichtfouse, E. (2020). Hemp Seed as a Source of Food Proteins. Sustainable Agriculture Reviews 42: Hemp Production and Applications, Springer International Publishing.","DOI":"10.1007\/978-3-030-41384-2"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Crini, G., and Lichtfouse, E. (2020). Traditional and New Applications of Hemp. Sustainable Agriculture Reviews 42: Hemp Production and Applications, Springer International Publishing.","DOI":"10.1007\/978-3-030-41384-2"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Agrawal, D.C., Kumar, R., and Dhanasekaran, M. (2022). Cannabis\/Hemp: Sustainable Uses, Opportunities, and Current Limitations. Cannabis\/Hemp for Sustainable Agriculture and Materials, Springer.","DOI":"10.1007\/978-981-16-8778-5"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"105864","DOI":"10.1016\/j.resconrec.2021.105864","article-title":"From Residue to Resource: The Multifaceted Environmental and Bioeconomy Potential of Industrial Hemp (Cannabis Sativa L.)","volume":"175","author":"Moscariello","year":"2021","journal-title":"Resour. Conserv. Recycl."},{"key":"ref_31","first-page":"177","article-title":"Integral Capitalization of Hemp Oils in Accordance with Circular Bio Economy Principles","volume":"20","author":"Petculescu","year":"2023","journal-title":"Agric. Econ. Rural. Dev."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Crini, G., and Lichtfouse, E. (2020). Hempseed Protein: Processing and Functional Properties. Sustainable Agriculture Reviews 42: Hemp Production and Applications, Springer International Publishing.","DOI":"10.1007\/978-3-030-41384-2"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Serrapica, F., Masucci, F., Raffrenato, E., Sannino, M., Vastolo, A., Barone, C.M.A., and Di Francia, A. (2019). High Fiber Cakes from Mediterranean Multipurpose Oilseeds as Protein Sources for Ruminants. Animals, 9.","DOI":"10.3390\/ani9110918"},{"key":"ref_34","unstructured":"Garc\u00eda-Tejero, I.F., and Dur\u00e1n-Zuazo, V.H. (2023). Chapter 13\u2014By-Products of Hemp from a Nutritional Point of View: New Perspectives and Opportunities. Current Applications, Approaches, and Potential Perspectives for Hemp, Academic Press."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e0248790","DOI":"10.1371\/journal.pone.0248790","article-title":"Hemp Seed (Cannabis sativa L.) Enriched Pasta: Physicochemical Properties and Quality Evaluation","volume":"16","author":"Teterycz","year":"2021","journal-title":"PLoS ONE"},{"key":"ref_36","first-page":"73","article-title":"Effects of Hemp Flour, Seeds And Oil Additions on Bread Quality","volume":"8","author":"Apostol","year":"2018","journal-title":"Int. J. Eng. Res. Appl."},{"key":"ref_37","unstructured":"AACC (2000). International Approved Methods of American Association of Cereal Chem-Ists."},{"key":"ref_38","unstructured":"(2024, July 24). Decision No. 68 Decision of the Government of the Republic of Moldova No. 68 of 29.01.2009. Regarding the Approval of the Technical Regulations \u201cFlour, Semolina and Cereal Bran\u201d. Available online: https:\/\/www.legis.md\/cautare\/getResults?doc_id=22139&lang=ro."},{"key":"ref_39","unstructured":"AOAC (2002). Association of Official Analytical Chemists (AOAC). Official Methods of Analysis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1002\/food.19970410211","article-title":"Importance and utilization of amaranth in food industry. Part 2. Composition of amino acids and fatty acids","volume":"41","author":"Dodok","year":"1997","journal-title":"Nahrung"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Siddiqi, R.A., Singh, T.P., Rani, M., Sogi, D.S., and Bhat, M.A. (2020). Diversity in Grain, Flour, Amino Acid Composition, Protein Profiling, and Proportion of Total Flour Proteins of Different Wheat Cultivars of North India. Front. Nutr., 7.","DOI":"10.3389\/fnut.2020.00141"},{"key":"ref_42","unstructured":"Food and Agriculture Organization of the United Nations (2013). Dietary Protein Quality Evaluation in Human Nutrition: Report of an FAO Expert Consultation, 31 March-2 April, 2011, Auckland, New Zealand, FAO Food and Nutrition Paper."},{"key":"ref_43","unstructured":"Ferrandi School of Culinary Arts, Nurra, R., Adedotun, H., Abramowitz-Moreau, C., Evans, A., Walsh, C., and Doux, R. (2017). French P\u00e2tisserie: Master Recipes and Techniques from the Ferrandi School of Culinary Arts, Flammarion. English-Language Edition."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.foodqual.2013.06.010","article-title":"Existing and New Approaches for the Analysis of CATA Data","volume":"30","author":"Meyners","year":"2013","journal-title":"Food Qual. Prefer."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1007\/s13197-010-0008-2","article-title":"Baking Loss of Bread with Special Emphasis on Increasing Water Holding Capacity","volume":"47","author":"Kotoki","year":"2010","journal-title":"J. Food Sci. Technol."},{"key":"ref_46","unstructured":"AACC (2010). 10-05.01 Guidelines for Measurement of Volume by Rapeseed Displacement, AACC Approved Methods of Analysis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3336","DOI":"10.1002\/fsn3.2323","article-title":"Biochemical and Nutritional Profile of Maize Bran-enriched Flour in Relation to Its End-use Quality","volume":"9","author":"Hussain","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_48","unstructured":"Nielsen, S.S. (2017). Color Analysis. Food Analysis, Springer International Publishing."},{"key":"ref_49","unstructured":"Sahin, S., and \u015eumnu, S.G. (2006). Physical Properties of Foods, Springer."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.ifset.2015.08.004","article-title":"Conversion of a Low Protein Hemp Seed Meal into a Functional Protein Concentrate through Enzymatic Digestion of Fibre Coupled with Membrane Ultrafiltration","volume":"31","author":"Malomo","year":"2015","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_51","unstructured":"Niccum, C. (2022). Can Pea and White Hemp Proteins Be Used as a More Sustainable Protein Source to Improve Nutritional and Sensory Results in Gluten-Free Baked Products?. [Ph.D. Thesis, London South Bank University]."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.foodchem.2007.06.064","article-title":"Characterization, Amino Acid Composition and in Vitro Digestibility of Hemp (Cannabis sativa L.) Proteins","volume":"107","author":"Wang","year":"2008","journal-title":"Food Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"14257","DOI":"10.1038\/s41598-021-93451-x","article-title":"In Vitro Protein Digestibility and Biochemical Characteristics of Soaked, Boiled and Fermented Soybeans","volume":"11","author":"Ketnawa","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1080\/10408399409527649","article-title":"Implications of Antinutritional Components in Soybean Foods","volume":"34","author":"Liener","year":"1994","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"131843","DOI":"10.1016\/j.foodchem.2021.131843","article-title":"Modification of Hemp Seed Protein Isolate (Cannabis sativa L.) by High-Intensity Ultrasound Treatment. Part 1: Functional Properties","volume":"375","author":"Karabulut","year":"2022","journal-title":"Food Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"809","DOI":"10.3945\/ajcn.111.017061","article-title":"Leucine-Enriched Essential Amino Acid Supplementation during Moderate Steady State Exercise Enhances Postexercise Muscle Protein Synthesis","volume":"94","author":"Pasiakos","year":"2011","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"2616S","DOI":"10.3945\/jn.116.234518","article-title":"Leucine and Mammalian Target of Rapamycin\u2013Dependent Activation of Muscle Protein Synthesis in Aging","volume":"146","year":"2016","journal-title":"J. Nutr."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Friedman, M. (2018). Absorption and Utilization of Amino Acids, CRC Press. CRC revivals; Facsimile edition.","DOI":"10.1201\/9781351069441"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2091","DOI":"10.1007\/s00726-017-2494-2","article-title":"The Role of Methionine on Metabolism, Oxidative Stress, and Diseases","volume":"49","author":"Li","year":"2017","journal-title":"Amino Acids"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.cytogfr.2017.05.004","article-title":"Amino-Acid Sensing and Degrading Pathways in Immune Regulation","volume":"35","author":"Grohmann","year":"2017","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1002\/fft2.441","article-title":"Functions of Immunologically Active Peptides and Their Roles in Intestinal Inflammation","volume":"6","author":"Wang","year":"2025","journal-title":"Food Front."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2032S","DOI":"10.1093\/jn\/138.10.2032S","article-title":"Comparative Aspects of Tissue Glutamine and Proline Metabolism","volume":"138","author":"Bertolo","year":"2008","journal-title":"J. Nutr."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Hole\u010dek, M. (2024). Origin and Roles of Alanine and Glutamine in Gluconeogenesis in the Liver, Kidneys, and Small Intestine under Physiological and Pathological Conditions. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25137037"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Grabacka, M., Pierzchalska, M., Dean, M., and Reiss, K. (2016). Regulation of Ketone Body Metabolism and the Role of PPAR\u03b1. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17122093"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1016\/j.bbadis.2013.05.003","article-title":"Ketogenic Essential Amino Acids Replacement Diet Ameliorated Hepatosteatosis with Altering Autophagy-Associated Molecules","volume":"1832","author":"Xu","year":"2013","journal-title":"Biochim. Biophys. Acta (BBA)\u2014Mol. Basis Dis."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.2337\/db07-0123","article-title":"Increasing Dietary Leucine Intake Reduces Diet-Induced Obesity and Improves Glucose and Cholesterol Metabolism in Mice via Multimechanisms","volume":"56","author":"Zhang","year":"2007","journal-title":"Diabetes"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"110931","DOI":"10.1016\/j.lwt.2021.110931","article-title":"Protein Fortification of Wheat Bread Using Dry Fractionated Chickpea Protein-Enriched Fraction or Its Sourdough","volume":"142","author":"Xing","year":"2021","journal-title":"LWT"},{"key":"ref_68","first-page":"304","article-title":"Properties and Nutritional Value of Wheat Bread Enriched by Hemp Products","volume":"9","year":"2015","journal-title":"Slovak J. Food Sci.\/Potravin."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.lwt.2018.12.028","article-title":"Hemp Flour as a Valuable Component for Enriching Physicochemical and Antioxidant Properties of Wheat Bread","volume":"102","author":"Mikulec","year":"2019","journal-title":"LWT"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"3230","DOI":"10.1111\/1750-3841.17128","article-title":"Effect of the Storage Process on Quality Characteristics of Hemp-enriched \u201cTsoureki\u201d a Rich-dough Baked Greek Product","volume":"89","author":"Lazou","year":"2024","journal-title":"J. Food Sci."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1556\/CRC.2012.0033","article-title":"Changes in Baking Quality of Composite Wheat\/Hemp Flour Detected by Means of Mixolab","volume":"41","year":"2013","journal-title":"Cereal Res. Commun."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Attia, Y.A., Al-Harthi, M.A., Korish, M.A., and Shiboob, M.H. (2020). Protein and Amino Acid Content in Four Brands of Commercial Table Eggs in Retail Markets in Relation to Human Requirements. Animals, 10.","DOI":"10.3390\/ani10030406"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1079\/BJN19940089","article-title":"Amino Acid Composition of the Milk of Some Mammalian Species Changes with Stage of Lactation","volume":"72","author":"Davis","year":"1994","journal-title":"Br. J. Nutr."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s002170100407","article-title":"Effect of Different Milling Methods on Chemical Composition of Whole Wheat Flour","volume":"213","author":"Prabhasankar","year":"2001","journal-title":"Eur. Food Res. Technol."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Tsochatzis, E., Papageorgiou, M., and Kalogiannis, S. (2019). Validation of a HILIC UHPLC-MS\/MS Method for Amino Acid Profiling in Triticum Species Wheat Flours. Foods, 8.","DOI":"10.3390\/foods8100514"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1017\/S0007114519002599","article-title":"Effect of Dietary Threonine on Growth Performance and Muscle Growth, Protein Synthesis and Antioxidant-Related Signalling Pathways of Hybrid Catfish Pelteobagrus Vachelli \u2640 \u00d7 Leiocassis Longirostris \u2642","volume":"123","author":"Zhao","year":"2020","journal-title":"Br. J. Nutr."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1093\/jas\/sky449","article-title":"Effects of Dietary Leucine Supplementation and Immune System Stimulation on Plasma AA Concentrations and Tissue Protein Synthesis in Starter Pigs1","volume":"97","author":"Rudar","year":"2019","journal-title":"J. Anim. Sci."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"644","DOI":"10.2174\/1389203720666190305163135","article-title":"Isoleucine Plays an Important Role for Maintaining Immune Function","volume":"20","author":"Gu","year":"2019","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"11801","DOI":"10.1021\/jf102636b","article-title":"Evaluating the Quality of Protein from Hemp Seed (Cannabis sativa L.) Products Through the Use of the Protein Digestibility-Corrected Amino Acid Score Method","volume":"58","author":"House","year":"2010","journal-title":"J. Agric. Food Chem."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Rusu, I.E., Marc (Vlaic), R.A., Mure\u015fan, C.C., Mure\u015fan, A.E., Mure\u015fan, V., Pop, C.R., Chi\u015f, M.S., Man, S.M., Filip, M.R., and Onica, B.-M. (2021). Hemp (Cannabis sativa L.) Flour-Based Wheat Bread as Fortified Bakery Product. Plants, 10.","DOI":"10.3390\/plants10081558"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Bonke, A., Sieuwerts, S., and Petersen, I.L. (2020). Amino Acid Composition of Novel Plant Drinks from Oat, Lentil and Pea. Foods, 9.","DOI":"10.3390\/foods9040429"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1007\/s11676-019-00882-4","article-title":"Amino Acid Profiles and Protein Quality of Siberian Apricot (Prunus sibirica L.) Kernels from Inner Mongolia","volume":"31","author":"Yin","year":"2020","journal-title":"J. For. Res."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Kawakatsu, T., and Takaiwa, F. (2019). Rice Proteins and Essential Amino Acids. Rice, Elsevier.","DOI":"10.1016\/B978-0-12-811508-4.00004-6"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1080\/17450390903217523","article-title":"The Effects of Supplementing a Low-Protein Threonine-Deficient Diet with Different Sources of Non-Essential Amino Acids on Nitrogen Retention and Gut Structure in Young Pigs","volume":"64","author":"Buraczewska","year":"2010","journal-title":"Arch. Anim. Nutr."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"3984","DOI":"10.1002\/jsfa.12351","article-title":"Utilization of Industrial Hemp By-product Defatted Seed Flour: Effect of Its Incorporation on the Properties and Quality Characteristics of \u2018Tsoureki\u2019, a Rich-dough Baked Greek Product","volume":"103","author":"Lazou","year":"2023","journal-title":"J. Sci. Food Agric."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Merlino, M., Tripodi, G., Cincotta, F., Prestia, O., Miller, A., Gattuso, A., Verzera, A., and Condurso, C. (2022). Technological, Nutritional, and Sensory Characteristics of Gnocchi Enriched with Hemp Seed Flour. Foods, 11.","DOI":"10.3390\/foods11182783"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Lu\u010dan \u010coli\u0107, M., Juki\u0107, M., Nakov, G., Lukinac, J., and Antunovi\u0107, M. (2024). Sustainable Utilization of Hemp Press Cake Flour in Ice Cream Production: Physicochemical, Rheological, Textural, and Sensorial Properties. 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