{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:26:21Z","timestamp":1790141181102,"version":"4.0.1"},"reference-count":84,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:00:00Z","timestamp":1746748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Comisi\u00f3n Interministerial de Ciencia y Tecnolog\u00eda Project","doi-asserted-by":"publisher","award":["PID2023-148814OB-C21"],"award-info":[{"award-number":["PID2023-148814OB-C21"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Comisi\u00f3n Interministerial de Ciencia y Tecnolog\u00eda Project","doi-asserted-by":"publisher","award":["MICINN\/FEDER"],"award-info":[{"award-number":["MICINN\/FEDER"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Spanish Ministry of Science, Innovation and Universities","award":["PID2023-148814OB-C21"],"award-info":[{"award-number":["PID2023-148814OB-C21"]}]},{"name":"Spanish Ministry of Science, Innovation and Universities","award":["MICINN\/FEDER"],"award-info":[{"award-number":["MICINN\/FEDER"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Roasted carob flour is a sustainable ingredient rich in dietary fiber, polyphenols, and pinitol, offering potential for both food and pharmaceutical applications. However, its high sugar content and the presence of undesirable compounds such as furans present challenges for its use in bread making. This study evaluated the effects of prolonged sourdough fermentation on roasted carob flour, with a focus on microbial dynamics and its functional and technological properties. Carob and carob\u2013wheat sourdoughs were prepared using a mixed starter culture comprising three lactic acid bacteria (Lactiplantibacillus plantarum, Fructilactobacillus sanfranciscensis, and Lactobacillus helveticus) and three yeast species (Saccharomyces cerevisiae, Kazachstania humilis, and Torulaspora delbrueckii). The sourdoughs underwent six consecutive refreshment cycles and were analyzed to determine their pH, microbial and biochemical composition, gassing power, and volatile organic compounds (VOCs). The carob\u2013wheat sourdough exhibited faster acidification and higher lactic acid bacteria (LAB) activity, resulting in a 90\u201398% reduction in the sugar content, compared to 60% in the carob sourdough. Microbial sequencing revealed that L. plantarum was the dominant species in all samples, while K. humilis and S. cerevisiae were enriched in carob and carob\u2013wheat sourdough, respectively. Both types of sourdough demonstrated effective leavening in bread dough without the addition of commercial yeast. Fermentation also modified the VOC profiles, increasing esters and alcohols while reducing acids, aldehydes, ketones, and furans. While the antioxidant activity showed a slight decline, the pinitol content remained unchanged. These findings suggest that extended sourdough fermentation, supported by multiple refreshments, enhances the baking suitability of roasted carob flour and supports its application as a functional, sustainable ingredient.<\/jats:p>","DOI":"10.3390\/foods14101677","type":"journal-article","created":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T08:20:23Z","timestamp":1746778823000},"page":"1677","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Effects of Sourdough Fermentation on the Biochemical Properties, Aroma Profile and Leavening Capacity of Carob Flour"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5191-9488","authenticated-orcid":false,"given":"Gemma","family":"Sanmart\u00edn","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de los Alimentos, Consejo Superior de Investigaciones Cient\u00edficas, Avda. Agust\u00edn Escardino, 7, 46980 Paterna, Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9677-0023","authenticated-orcid":false,"given":"Jose A.","family":"Prieto","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de los Alimentos, Consejo Superior de Investigaciones Cient\u00edficas, Avda. Agust\u00edn Escardino, 7, 46980 Paterna, Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6213-6881","authenticated-orcid":false,"given":"Miguel","family":"Morard","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de los Alimentos, Consejo Superior de Investigaciones Cient\u00edficas, Avda. Agust\u00edn Escardino, 7, 46980 Paterna, Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Estruch","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Universitat de Val\u00e8ncia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8207-1137","authenticated-orcid":false,"given":"Josep","family":"Blasco-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de los Alimentos, Consejo Superior de Investigaciones Cient\u00edficas, Avda. Agust\u00edn Escardino, 7, 46980 Paterna, Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Randez-Gil","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Instituto de Agroqu\u00edmica y Tecnolog\u00eda de los Alimentos, Consejo Superior de Investigaciones Cient\u00edficas, Avda. Agust\u00edn Escardino, 7, 46980 Paterna, Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,9]]},"reference":[{"key":"ref_1","unstructured":"MAPA (2023). Anuario de Estad\u00edstica Agraria, Ministerio de Agricultura, Pesca y Alimentaci\u00f3n. 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