{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:03:14Z","timestamp":1787670194256,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program project","award":["2022YFD1600303"],"award-info":[{"award-number":["2022YFD1600303"]}]},{"name":"National Key Research and Development Program project","award":["YYLH2307"],"award-info":[{"award-number":["YYLH2307"]}]},{"name":"National Key Research and Development Program project","award":["YBXM2417"],"award-info":[{"award-number":["YBXM2417"]}]},{"name":"National Key Research and Development Program project","award":["2023"],"award-info":[{"award-number":["2023"]}]},{"name":"National Key Research and Development Program project","award":["HAAS2024KJCX01"],"award-info":[{"award-number":["HAAS2024KJCX01"]}]},{"name":"Nanfan special project of Chinese Academy of Agricultural Sciences","award":["2022YFD1600303"],"award-info":[{"award-number":["2022YFD1600303"]}]},{"name":"Nanfan special project of Chinese Academy of Agricultural Sciences","award":["YYLH2307"],"award-info":[{"award-number":["YYLH2307"]}]},{"name":"Nanfan special project of Chinese Academy of Agricultural Sciences","award":["YBXM2417"],"award-info":[{"award-number":["YBXM2417"]}]},{"name":"Nanfan special project of Chinese Academy of Agricultural Sciences","award":["2023"],"award-info":[{"award-number":["2023"]}]},{"name":"Nanfan special project of Chinese Academy of Agricultural Sciences","award":["HAAS2024KJCX01"],"award-info":[{"award-number":["HAAS2024KJCX01"]}]},{"name":"Xinjiang Uygur Autonomous Region \u201cTianchi Talent\u201d Training Plan Project","award":["2022YFD1600303"],"award-info":[{"award-number":["2022YFD1600303"]}]},{"name":"Xinjiang Uygur Autonomous Region \u201cTianchi Talent\u201d Training Plan Project","award":["YYLH2307"],"award-info":[{"award-number":["YYLH2307"]}]},{"name":"Xinjiang Uygur Autonomous Region \u201cTianchi Talent\u201d Training Plan Project","award":["YBXM2417"],"award-info":[{"award-number":["YBXM2417"]}]},{"name":"Xinjiang Uygur Autonomous Region \u201cTianchi Talent\u201d Training Plan Project","award":["2023"],"award-info":[{"award-number":["2023"]}]},{"name":"Xinjiang Uygur Autonomous Region \u201cTianchi Talent\u201d Training Plan Project","award":["HAAS2024KJCX01"],"award-info":[{"award-number":["HAAS2024KJCX01"]}]},{"name":"Science and technology innovation project of Hainan Academy of Agricultural Sciences","award":["2022YFD1600303"],"award-info":[{"award-number":["2022YFD1600303"]}]},{"name":"Science and technology innovation project of Hainan Academy of Agricultural Sciences","award":["YYLH2307"],"award-info":[{"award-number":["YYLH2307"]}]},{"name":"Science and technology innovation project of Hainan Academy of Agricultural Sciences","award":["YBXM2417"],"award-info":[{"award-number":["YBXM2417"]}]},{"name":"Science and technology innovation project of Hainan Academy of Agricultural Sciences","award":["2023"],"award-info":[{"award-number":["2023"]}]},{"name":"Science and technology innovation project of Hainan Academy of Agricultural Sciences","award":["HAAS2024KJCX01"],"award-info":[{"award-number":["HAAS2024KJCX01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Identifying effective adjuvants to prevent and alleviate the adverse effects of chemotherapy remains a critical challenge in cancer therapy. This study investigated the protective effects of longan polysaccharide (LP) against cyclophosphamide-induced immunosuppression and oxidative stress in mice. Our findings revealed that LP administration significantly improved systemic immune function, as evidenced by marked increases in serum immunoglobulin levels (IgG2a: 1.82-fold, IgG2b: 1.46-fold, IgM: 1.26-fold, and IgG1: 1.22-fold) and key cytokines (IL-10: 1.53-fold, IL-12: 1.22-fold, and IFN-\u03b3: 1.20-fold), accompanied by substantial reductions in pro-inflammatory mediators (TGF-\u03b21: 28.72% decrease and IL-21: 36.28% decrease). Concurrently, LP restored oxidative balance by increasing SOD, GSH, and NO levels in multiple organs (liver, kidneys, and small intestine) and serum. Mechanistic studies using an in vitro Caco-2\/RAW264.7 coculture system revealed that four purified LP fractions (LPIa-LPIVa) effectively suppressed NF-\u03baB pathway activation through downregulation of TLR4 expression, reduction of the p-I\u03baB-\u03b1\/I\u03baB-\u03b1 ratio, and inhibition of nuclear NF-\u03baB translocation. These molecular effects correlated with decreased production of inflammatory mediators (TNF-\u03b1, IL-6, IL-8, iNOS, and NO). Collectively, these findings provide compelling evidence that LP possesses dual immunomodulatory and antioxidant capabilities, highlighting its potential as a natural adjuvant for alleviating chemotherapy-induced side effects.<\/jats:p>","DOI":"10.3390\/foods14162901","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T08:02:44Z","timestamp":1755763364000},"page":"2901","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Longan Polysaccharide as Adjuvant for Cyclophosphamide-Induced Side Effects in Murine Model"],"prefix":"10.3390","volume":"14","author":[{"given":"Yajuan","family":"Bai","sequence":"first","affiliation":[{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China"},{"name":"National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China"},{"name":"Institute of Western Agriculture, Chinese Academy of Agricultural Sciences (CAAS), Changji 831100, China"},{"name":"Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Fan","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China"},{"name":"National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China"},{"name":"Institute of Western Agriculture, Chinese Academy of Agricultural Sciences (CAAS), Changji 831100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengzhong","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China"},{"name":"National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China"},{"name":"Institute of Western Agriculture, Chinese Academy of Agricultural Sciences (CAAS), Changji 831100, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/s10565-006-0078-0","article-title":"Pathophysiological aspects of cyclophosphamide and ifosfamide induced hemorrhagic cystitis; implication of reactive oxygen and nitrogen species as well as PARP activation","volume":"23","author":"Korkmaz","year":"2007","journal-title":"Cell Biol. 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