{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T16:54:18Z","timestamp":1774457658345,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32301994"],"award-info":[{"award-number":["32301994"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Outstanding Young Scientist Program","award":["JWZQ2024101019"],"award-info":[{"award-number":["JWZQ2024101019"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Foods"],"abstract":"<jats:p>Radiation-induced intestinal injury (RIII), a severe complication of abdominopelvic radiotherapy, causes intestinal ischemia, ulcers, and necrosis, severely impacting patients\u2019 quality of life. Currently, effective treatments are limited, and a specific cure remains elusive. Our previous research showed that lactoferrin (LF) can promote intestinal stem cell (ISC) proliferation and tissue repair; however, its oral administration is limited by rapid degradation in the gastric environment. In this study, we developed LF-loaded liposomal nanoparticles (Lip-LF) using a simple ethanol injection method. The optimal formulation (cholesterol\/egg yolk lecithin ratio 2:8, LF concentration 12.5 mg\/mL) achieved a drug-loading capacity of 6.8% and a narrow size distribution (0.2 &lt; PDI &lt; 0.4). In vitro experiments demonstrated that Lip-LF protected LF from pepsin degradation in simulated gastric fluid (SGF), retaining over 80% integrity after 120 min, while releasing in simulated intestinal fluid (SIF). In vivo imaging revealed prolonged gastrointestinal retention of Lip-LF compared to free LF. In a murine model of RIII (12 Gy whole-body irradiation), Lip-LF significantly restored villus counts, increased crypt height, and promoted goblet-cell regeneration. Immunohistochemical and qPCR analyses revealed enhanced ISCs proliferation and upregulation of repair-associated genes, including Pcna and Olfm4. These findings demonstrate that Lip-LF protects LF from gastric degradation and enhances its targeted delivery to the intestine, improving its therapeutic efficacy in repairing RIII. Lip-LF thus offers a promising strategy for managing RIII.<\/jats:p>","DOI":"10.3390\/foods14193410","type":"journal-article","created":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T08:20:28Z","timestamp":1759393228000},"page":"3410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Lactoferrin-Loaded Liposomal Nanoparticles: Enhanced Intestinal Stability and Bioactivity for Mitigating Radiation-Induced Intestinal Injury"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4404-8390","authenticated-orcid":false,"given":"Yingying","family":"Lin","sequence":"first","affiliation":[{"name":"Key Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, NO. 10 Tianxiu Road, Haidian, Beijing 100191, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Ding","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, NO. 10 Tianxiu Road, Haidian, Beijing 100191, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yimeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, NO. 10 Tianxiu Road, Haidian, Beijing 100191, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sijia","family":"Song","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, NO. 10 Tianxiu Road, Haidian, Beijing 100191, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiyuan","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Dairy, Department of Nutrition and Health, China Agricultural University, NO. 10 Tianxiu Road, Haidian, Beijing 100191, China"},{"name":"Key Laboratory of Precision Nutrition and Food Quality, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"},{"name":"Food Laboratory of Zhongyuan, Luohe 462300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Fan, J., Lin, B., Fan, M., Niu, T., Gao, F., Tan, B., and Du, X. 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