{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T09:59:37Z","timestamp":1761731977075,"version":"build-2065373602"},"reference-count":48,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T00:00:00Z","timestamp":1761264000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003569","name":"Ministry of Food and Drug Safety","doi-asserted-by":"publisher","award":["23192MFDS282"],"award-info":[{"award-number":["23192MFDS282"]}],"id":[{"id":"10.13039\/501100003569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Natamycin is widely used in other countries for the postharvest treatment of agricultural commodities to prevent fungal growth. However, since no MRL has been set in Korea, natamycin residues are regulated under the Positive List System (PLS) with a uniform limit of 0.01 mg\/kg, requiring the development of highly sensitive and reliable analytical methods. In this study, a QuEChERS-based analytical method was developed and validated for the quantification of natamycin in five agricultural commodities\u2014soybean, mandarin, hulled rice, green pepper, and potato\u2014using liquid chromatography\u2013tandem mass spectrometry (LC-MS\/MS). Extraction using methanol with 3 g of MgSO4 resulted in high recoveries without crystallization, and clean-up with MgSO4 and C18 effectively reduced matrix interferences blow &lt;50%. Natamycin was detected in all five matrices at 6.8 min without any interfering peaks. The MLOQ was determined at 0.01 mg\/kg for all five matrices. The mean recoveries (82.2\u2013115.4%) and %CV values (1.1\u20134.6%) values were within the acceptance criteria defined by the CODEX guidelines. Matrix effects were classified as \u201csoft\u201d for mandarin (|ME| &lt; 20%) and \u201cmedium\u201d for soybean, hulled rice, green pepper, and potato (20% \u2264 |ME| &lt; 50%). The analytical method for natamycin was validated as suitable for regulatory safety monitoring under the Korean PLS.<\/jats:p>","DOI":"10.3390\/foods14213636","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T02:23:28Z","timestamp":1761704608000},"page":"3636","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Validation of a QuEChERS-Based LC\u2013MS\/MS Method for Natamycin in Imported Agricultural Commodities in Korea"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6058-0487","authenticated-orcid":false,"given":"Ga-Eul-Hae","family":"An","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Engineering, Graduate School, Hoseo University, Asan 31499, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2937-7965","authenticated-orcid":false,"given":"Joon-Kyung","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Engineering, Graduate School, Hoseo University, Asan 31499, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5876-3564","authenticated-orcid":false,"given":"Jae-Hyeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Engineering, Graduate School, Hoseo University, Asan 31499, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0307-7703","authenticated-orcid":false,"given":"Hee-Ra","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Engineering, Graduate School, Hoseo University, Asan 31499, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1094\/PDIS-12-17-2033-RE","article-title":"Natamycin, a New Biofungicide for Managing Crown Rot of Strawberry Caused by QoI-Resistant Colletotrichum acutatum","volume":"102","author":"Haack","year":"2018","journal-title":"Plant Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1007\/s10068-021-00981-1","article-title":"Natamycin: A natural preservative for food applications-a review","volume":"30","author":"Meena","year":"2021","journal-title":"Food Sci. 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