{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T16:55:56Z","timestamp":1782924956440,"version":"3.54.5"},"reference-count":92,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T00:00:00Z","timestamp":1762300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Health","award":["IZS PLV 01\/24 RC"],"award-info":[{"award-number":["IZS PLV 01\/24 RC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Listeria monocytogenes is a foodborne pathogen of significant public health concern due to its high environmental resilience and ability to cause severe infections in vulnerable populations. The objective of the present study is to characterize foodborne strains of Listeria monocytogenes isolated between 2020 and 2024 in northwestern Italy. Lm was detected through isolation, biochemical confirmation and molecular serogrouping. Next generation sequencing (NGS) analysis was used to characterize the strains in terms of virulence and antibiotic resistance. A total of 39 positive samples were identified from various food matrices, including meat products, fish, cheeses and ready-to-eat foods. The most frequently detected serogroups were IIc and IIa, with a notable presence of the highly virulent IVb group. Next-generation sequencing (NGS) was applied to all isolates, revealing the presence of virulence genes associated with the LIPI-1 island and internalins. In addition to pathogenicity islands, genes related to stress resistance (clpCEP, Gad A, GadB, GadC), biofilm production (agrA, flaA, degU, hfq) and sortase-mediated anchoring of surface protein (strA, strB) have been identified. The presence of antibiotic resistance genes was confirmed, with all isolates harboring the fosX gene. Moreover, four isolates exhibited resistance determinants against antibiotics belonging to two different classes: tetracyclines (tetM) and lincosamides (lsa(A)). Multilocus sequence typing (MLST) showed that clonal complex CC9 was the most prevalent among the isolates. Further, cgMLST and SNP analyses identified a principal cluster of closely related strains, which were isolated from meat products. These findings highlight the need for continuous surveillance of L. monocytogenes.<\/jats:p>","DOI":"10.3390\/foods14213788","type":"journal-article","created":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T13:19:29Z","timestamp":1762348769000},"page":"3788","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Investigation of Listeria monocytogenes in Food in Northwestern Italy (2020\u20132024)"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5871-217X","authenticated-orcid":false,"given":"Monica","family":"Pitti","sequence":"first","affiliation":[{"name":"SC Sicurezza Alimentare, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d\u2019Aosta (IZSPLV), Via Bologna 148, 10154 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5965-2423","authenticated-orcid":false,"given":"Matteo","family":"Tavecchia","sequence":"additional","affiliation":[{"name":"SC Sicurezza Alimentare, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d\u2019Aosta (IZSPLV), Via Bologna 148, 10154 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6976-803X","authenticated-orcid":false,"given":"Angelo","family":"Romano","sequence":"additional","affiliation":[{"name":"SC Sicurezza Alimentare, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d\u2019Aosta (IZSPLV), Via Bologna 148, 10154 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Carrella","sequence":"additional","affiliation":[{"name":"SC Sicurezza Alimentare, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d\u2019Aosta (IZSPLV), Via Bologna 148, 10154 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Previto","sequence":"additional","affiliation":[{"name":"SC Sicurezza Alimentare, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d\u2019Aosta (IZSPLV), Via Bologna 148, 10154 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Manila","family":"Bianchi","sequence":"additional","affiliation":[{"name":"SC Sicurezza Alimentare, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d\u2019Aosta (IZSPLV), Via Bologna 148, 10154 Turin, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1007\/s10482-024-02027-y","article-title":"Phylogenomic analyses of the Listeriaceae family support species reclassification and proposal of a new family and new genera","volume":"118","author":"Bouznada","year":"2025","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3910","DOI":"10.1111\/1541-4337.13219","article-title":"Listeria monocytogenes in Food Businesses: From Persistence Strategies to Intervention\/Prevention Strategies\u2014A Review","volume":"22","author":"Tuytschaever","year":"2023","journal-title":"Comp. Rev. Food Sci. Food Safe"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Schoder, D., Guldimann, C., and M\u00e4rtlbauer, E. (2022). Asymptomatic Carriage of Listeria monocytogenes by Animals and Humans and Its Impact on the Food Chain. Foods, 11.","DOI":"10.3390\/foods11213472"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Wiktorczyk-Kapischke, N., Skowron, K., Grudlewska-Buda, K., Wa\u0142ecka-Zacharska, E., Korkus, J., and Gospodarek-Komkowska, E. (2021). Adaptive Response of Listeria monocytogenes to the Stress Factors in the Food Processing Environment. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.710085"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Centorotola, G., Ziba, M.W., Cornacchia, A., Chiaverini, A., Torresi, M., Guidi, F., Camm\u00e0, C., Bowa, B., Mtonga, S., and Magambwa, P. (2023). Listeria monocytogenes in Ready to Eat Meat Products from Zambia: Phenotypical and Genomic Characterization of Isolates. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1228726"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Castrica, M., Andoni, E., Intraina, I., Curone, G., Copelotti, E., Massacci, F.R., Terio, V., Colombo, S., and Balzaretti, C.M. (2021). Prevalence of Listeria monocytogenes and Salmonella spp. in Different Ready to Eat Foods from Large Retailers and Canteens over a 2-Year Period in Northern Italy. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph182010568"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Di Ciccio, P., Rubiola, S., Panebianco, F., Lomonaco, S., Allard, M., Bianchi, D.M., Civera, T., and Chiesa, F. (2022). Biofilm Formation and Genomic Features of Listeria monocytogenes Strains Isolated from Meat and Dairy Industries Located in Piedmont (Italy). Int. J. Food Microbiol., 378.","DOI":"10.1016\/j.ijfoodmicro.2022.109784"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Romeo, M., Lasagabaster, A., Lavilla, M., and Am\u00e1rita, F. (2025). Genetic Diversity, Biofilm Formation, and Antibiotic Resistance in Listeria monocytogenes Isolated from Meat-Processing Plants. Foods, 14.","DOI":"10.3390\/foods14091580"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Osek, J., Lachtara, B., and Wieczorek, K. (2022). Listeria monocytogenes\u2014How This Pathogen Survives in Food-Production Environments?. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.866462"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.1080\/21505594.2021.1975526","article-title":"Pathogenicity and Virulence of Listeria monocytogenes: A Trip from Environmental to Medical Microbiology","volume":"12","author":"Quereda","year":"2021","journal-title":"Virulence"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"112040","DOI":"10.1016\/j.foodres.2022.112040","article-title":"Genetic Diversity, Antibiotic Resistance, and Virulence Profiles of Listeria monocytogenes from Retail Meat and Meat Processing","volume":"162","author":"Li","year":"2022","journal-title":"Food Res. Int."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Reis, J.O., Teixeira, L.A.C., Cunha-Neto, A., Castro, V.S., and Figueiredo, E.E.S. (2024). Listeria monocytogenes in Beef: A Hidden Risk. Res. Microbiol., 175.","DOI":"10.1016\/j.resmic.2024.104215"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e06169","DOI":"10.1016\/j.heliyon.2021.e06169","article-title":"Occurrence, Antimicrobial Resistance, Serotyping and Virulence Genes of Listeria monocytogenes Isolated from Foods","volume":"7","author":"Bouymajane","year":"2021","journal-title":"Heliyon"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (2024). Prolonged Multi-country Outbreak of Listeria monocytogenes ST1607 Linked to Smoked Salmon Products. EFSA J., 21, 8810E.","DOI":"10.2903\/sp.efsa.2024.EN-8810"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (2024). Prolonged Multi-country Outbreak of Listeria monocytogenes ST173 Linked to Consumption of Fish Products. EFSA J., 21, 8885E.","DOI":"10.2903\/sp.efsa.2024.EN-8885"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (2023). Prolonged Multi-country Cluster of Listeria monocytogenes ST155 Infections Linked to Ready-to-eat Fish Products. EFSA J., 20, 8538E.","DOI":"10.2903\/sp.efsa.2023.EN-8538"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ijmm.2010.05.002","article-title":"Listeria monocytogenes Lineages: Genomics, Evolution, Ecology, and Phenotypic Characteristics","volume":"301","author":"Orsi","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_18","unstructured":"Commission Regulation (2025, October 22). (EC) No 2073\/2005 of 15 November 2005 on Microbiological Criteria for Foodstuffs. Available online: https:\/\/eur-lex.europa.eu\/eli\/reg\/2005\/2073\/oj\/eng."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (2025). The European Union One Health 2023 Zoonoses Report. EFSA J., 22, e9106.","DOI":"10.2903\/j.efsa.2024.9106"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"102","DOI":"10.3343\/alm.2018.38.2.102","article-title":"Increasing Incidence of Listeriosis and Infection-Associated Clinical Outcomes","volume":"38","author":"Choi","year":"2018","journal-title":"Ann. Lab. Med."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Luciani, M., Krasteva, I., Schirone, M., D\u2019Onofrio, F., Iannetti, L., Torresi, M., Di Pancrazio, C., Perletta, F., Valentinuzzi, S., and Tittarelli, M. (2025). Adaptive Strategies of Listeria monocytogenes: An in-Depth Analysis of the Virulent Strain Involved in an Outbreak in Italy through Quantitative Proteomics. Int. J. Food Microbiol., 427.","DOI":"10.1016\/j.ijfoodmicro.2024.110951"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1038\/nrmicro.2017.126","article-title":"Listeria monocytogenes: Towards a Complete Picture of Its Physiology and Pathogenesis","volume":"16","author":"Radoshevich","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1128\/mr.55.3.476-511.1991","article-title":"Listeria monocytogenes, a Food-Borne Pathogen","volume":"55","author":"Farber","year":"1991","journal-title":"Microbiol. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Lecuit, M. (2020). Listeria monocytogenes a Model in Infection Biology. Cell. Microbiol., 22.","DOI":"10.1111\/cmi.13186"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Davis, M.L., Ricke, S.C., and Donaldson, J.R. (2019). Establishment of Listeria monocytogenes in the Gastrointestinal Tract. Microorganisms, 7.","DOI":"10.3390\/microorganisms7030075"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Sibanda, T., and Buys, E.M. (2022). Listeria monocytogenes Pathogenesis: The Role of Stress Adaptation. Microorganisms, 10.","DOI":"10.3390\/microorganisms10081522"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"146","DOI":"10.4415\/ANN_12_02_07","article-title":"Listeria monocytogenes Serotypes in Human Infections (Italy, 2000\u20132010)","volume":"48","author":"Pontello","year":"2012","journal-title":"Ann. Dell'istituto Super. Sanit\u00e0"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"379","DOI":"10.4161\/viru.2.5.17703","article-title":"The Arsenal of Virulence Factors Deployed by Listeria monocytogenes to Promote Its Cell Infection Cycle","volume":"2","author":"Camejo","year":"2011","journal-title":"Virulence"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Wiktorczyk-Kapischke, N., Skowron, K., and Wa\u0142ecka-Zacharska, E. (2023). Genomic and Pathogenicity Islands of Listeria monocytogenes\u2014Overview of Selected Aspects. Front. Mol. Biosci., 10.","DOI":"10.3389\/fmolb.2023.1161486"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Song, Y., Gao, B., Cai, H., Qin, X., Xia, X., Dong, Q., Hirata, T., and Li, Z. (2025). Comparative Analysis of Virulence in Listeria monocytogenes: Insights from Genomic Variations and in Vitro Cell-Based Studies. Int. J. Food Microbiol., 435.","DOI":"10.1016\/j.ijfoodmicro.2025.111188"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1007\/s00284-019-01671-2","article-title":"Association of Listeria monocytogenes LIPI-1 and LIPI-3 Marker llsX with Invasiveness","volume":"76","year":"2019","journal-title":"Curr. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Elsayed, M.E., Abd El-Hamid, M.I., El-Gedawy, A., Bendary, M.M., ELTarabili, R.M., Alhomrani, M., Alamri, A.S., Alghamdi, S.A., Arnout, M., and Binjawhar, D.N. (2022). New Insights into Listeria monocytogenes Antimicrobial Resistance, Virulence Attributes and Their Prospective Correlation. Antibiotics, 11.","DOI":"10.3390\/antibiotics11101447"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"100127","DOI":"10.1016\/j.medmic.2025.100127","article-title":"The Past, Present, Future of Listeria monocytogenes: Understanding the Molecular Pathways, Antibiotic Resistance and Public Health Implications","volume":"25","author":"Anupama","year":"2025","journal-title":"Med. Microecol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1111\/1541-4337.12387","article-title":"Emergence of Antibiotic Resistance in Listeria monocytogenes Isolated from Food Products: A Comprehensive Review","volume":"17","author":"Olaimat","year":"2018","journal-title":"Comp. Rev. Food Sci. Food Safe"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"5907","DOI":"10.1007\/s00203-021-02590-2","article-title":"Listeria monocytogenes: Health Risk and a Challenge for Food Processing Establishments","volume":"203","author":"Biduski","year":"2021","journal-title":"Arch. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"P\u00e9rez-Rodr\u00edguez, F., and Mercanoglu Taban, B. (2019). A State-of-Art Review on Multi-Drug Resistant Pathogens in Foods of Animal Origin: Risk Factors and Mitigation Strategies. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02091"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2825","DOI":"10.1002\/fsn3.2910","article-title":"Listeria monocytogenes in Foods\u2014From Culture Identification to Whole-genome Characteristics","volume":"10","author":"Osek","year":"2022","journal-title":"Food Sci. Nutr."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"100003","DOI":"10.1016\/j.jfp.2022.10.002","article-title":"Wholegenome Sequencing as the Gold Standard Approach for Control of Listeria monocytogenes in the Food Chain","volume":"86","author":"Lakicevic","year":"2023","journal-title":"J. Food Prot."},{"key":"ref_39","unstructured":"(2017). Microbiology of the Food Chain\u2014Horizontal Method for the Detection and Enumeration of Listeria monocytogenes and of Listeria spp.\u2014Part 1: Detection Method (Standard No. ISO 11290-1:2017). Available online: https:\/\/www.iso.org\/standard\/60313.html."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3819","DOI":"10.1128\/JCM.42.8.3819-3822.2004","article-title":"Differentiation of the Major Listeria monocytogenes Serovars by Multiplex PCR","volume":"42","author":"Doumith","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.4315\/0362-028X-67.8.1646","article-title":"A Validated PCR-Based Method to Detect Listeria monocytogenes Using Raw Milk as a Food Model\u2014Towards an International Standard","volume":"67","author":"Wagner","year":"2004","journal-title":"J. Food Prot."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Romano, A., Carrella, S., Rezza, S., Nia, Y., Hennekinne, J.A., Bianchi, D.M., Martucci, F., Zuccon, F., Gulino, M., and Di Mari, C. (2023). First Report of Food Poisoning Due to Staphylococcal Enterotoxin Type B in D\u00f6ner Kebab (Italy). Pathogens, 12.","DOI":"10.3390\/pathogens12091139"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3491","DOI":"10.1093\/jac\/dkaa345","article-title":"ResFinder 4.0 for Predictions of Phenotypes from Genotypes","volume":"75","author":"Bortolaia","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Clausen, P.T.L.C., Aarestrup, F.M., and Lund, O. (2018). Rapid and Precise Alignment of Raw Reads against Redundant Databases with KMA. BMC Bioinform., 19.","DOI":"10.1186\/s12859-018-2336-6"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e00570-23","DOI":"10.1128\/jcm.00570-23","article-title":"Construction of the ETECFinder Database for the Characterization of Enterotoxigenic Escherichia coli (ETEC) and Revision of the VirulenceFinder Web Tool at the CGE Website","volume":"62","author":"Scheutz","year":"2024","journal-title":"J. Clin. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1501","DOI":"10.1128\/JCM.03617-13","article-title":"Real-Time Whole-Genome Sequencing for Routine Typing, Surveillance, and Outbreak Detection of Verotoxigenic Escherichia coli","volume":"52","author":"Joensen","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e01269-20","DOI":"10.1128\/JCM.01269-20","article-title":"In Silico Genotyping of Escherichia coli Isolates for Extraintestinal Virulence Genes by Use of Whole-Genome Sequencing Data","volume":"58","author":"Johnson","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e02229-21","DOI":"10.1128\/jcm.02229-21","article-title":"Characterization of Atypical Shiga Toxin Gene Sequences and Description of Stx2j, a New Subtype","volume":"60","author":"Gill","year":"2022","journal-title":"J. Clin. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Lindsey, R.L., Prasad, A., Feldgarden, M., Gonzalez-Escalona, N., Kapsak, C., Klimke, W., Melton-Celsa, A., Smith, P., Souvorov, A., and Truong, J. (2023). Identification and Characterization of Ten Escherichia coli Strains Encoding Novel Shiga Toxin 2 Subtypes, Stx2n as Well as Stx2j, Stx2m, and Stx2o, in the United States. Microorganisms, 11.","DOI":"10.20944\/preprints202309.1481.v1"},{"key":"ref_50","unstructured":"Posit Software (2025). Posit Team RStudio: Integrated Development Environment for R 2025, PBC. Available online: http:\/\/www.posit.co\/."},{"key":"ref_51","unstructured":"(2025, July 22). Genpat (Italian National Reference Centre for WGS of Microbial Pathogens). Available online: https:\/\/genpat.izs.it\/cmdbuild\/ui\/#custompages\/welcomePage."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1101\/gr.232397.117","article-title":"GrapeTree: Visualization of Core Genomic Relationships among 100,000 Bacterial Pathogens","volume":"28","author":"Zhou","year":"2018","journal-title":"Genome Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1635","DOI":"10.1093\/molbev\/msw046","article-title":"ETE 3: Reconstruction, Analysis, and Visualization of Phylogenomic Data","volume":"33","author":"Serra","year":"2016","journal-title":"Mol. Biol. Evol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Herfort, B., Lautenbach, S., Porto De Albuquerque, J., Anderson, J., and Zipf, A. (2021). The Evolution of Humanitarian Mapping within the OpenStreetMap Community. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-82404-z"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"100307","DOI":"10.1016\/j.jfp.2024.100307","article-title":"Prevalence, Genotypic Characteristics, and Antibiotic Resistance of Listeria monocytogenes from Retail Foods in Huzhou, China","volume":"87","author":"Zhang","year":"2024","journal-title":"J. Food Prot."},{"key":"ref_56","first-page":"1","article-title":"SPREAD: Spatiotemporal Pathogen Relationships and Epidemiological Analysis Dashboard","volume":"60","year":"2024","journal-title":"Vet. Ital."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Biological Hazards (BIOHAZ), Ricci, A., Allende, A., Bolton, D., Chemaly, M., Davies, R., Fern\u00e1ndez Esc\u00e1mez, P.S., Girones, R., Herman, L., and Koutsoumanis, K. (2018). Listeria monocytogenes Contamination of Ready-to-eat Foods and the Risk for Human Health in the EU. EFSA J., 16, e05134.","DOI":"10.2903\/j.efsa.2018.5134"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.4315\/0362-028X-69.7.1533","article-title":"Survival of Listeria monocytogenes in Uncooked Italian Dry Sausage (Salami)","volume":"69","author":"Gianfranceschi","year":"2006","journal-title":"J. Food Prot."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.fm.2009.03.003","article-title":"Distribution of Serotypes and Pulsotypes of Listeria monocytogenes from Human, Food and Environmental Isolates (Italy 2002\u20132005)","volume":"26","author":"Gianfranceschi","year":"2009","journal-title":"Food Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Cheng, J., Wu, S., Ye, Q., Gu, Q., Zhang, Y., Ye, Q., Lin, R., Liang, X., Liu, Z., and Bai, J. (2024). A Novel Multiplex PCR Based Method for the Detection of Listeria monocytogenes Clonal Complex 8. Int. J. Food Microbiol., 409.","DOI":"10.1016\/j.ijfoodmicro.2023.110475"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/ng.3501","article-title":"Uncovering Listeria monocytogenes Hypervirulence by Harnessing Its Biodiversity","volume":"48","author":"Maury","year":"2016","journal-title":"Nat. Genet."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"F\u00e9lix, B., Feurer, C., Maillet, A., Guillier, L., Boscher, E., Kerouanton, A., Denis, M., and Roussel, S. (2018). Population Genetic Structure of Listeria monocytogenes Strains Isolated from the Pig and Pork Production Chain in France. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.00684"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"271","DOI":"10.4315\/0362-028X.JFP-15-599","article-title":"Listeria monocytogenes in Smoked Salmon and Other Smoked Fish at Retail in Italy: Frequency of Contamination and Strain Characterization in Products from Different Manufacturers","volume":"80","author":"Acciari","year":"2017","journal-title":"J. Food Prot."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.fm.2004.04.004","article-title":"Prevalence and Characterization of Listeria monocytogenes Isolated from Soft Cheese","volume":"22","author":"Pintado","year":"2005","journal-title":"Food Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.fm.2004.01.011","article-title":"Characterization of Listeria monocytogenes Strains Isolated from Gorgonzola Cheese Rinds","volume":"21","author":"Carminati","year":"2004","journal-title":"Food Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"4019","DOI":"10.1111\/1541-4337.12768","article-title":"The Occurrence, Growth, and Biocontrol of Listeria monocytogenes in Fresh and Surface-ripened Soft and Semisoft Cheeses","volume":"20","author":"Falardeau","year":"2021","journal-title":"Comp. Rev. Food. Sci. Food Safe"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Camellini, S., Iseppi, R., Cond\u00f2, C., and Messi, P. (2021). Ready-to-Eat Sandwiches as Source of Pathogens Endowed with Antibiotic Resistance and Other Virulence Factors. Appl. Sci., 11.","DOI":"10.3390\/app11167177"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority, and European Centre for Disease Prevention and Control (2018). Multi-country Outbreak of Listeria monocytogenes Serogroup IVb, Multi-locus Sequence Type 6, Infections Probably Linked to Frozen Corn. EFSA Support. Publ., 15, EN-1402.","DOI":"10.2903\/sp.efsa.2018.EN-1402"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Kuch, A., Goc, A., Belkiewicz, K., Filipello, V., Ronkiewicz, P., Go\u0142\u0119biewska, A., Wr\u00f3bel, I., Kiedrowska, M., Wa\u015bko, I., and Hryniewicz, W. (2018). Molecular Diversity and Antimicrobial Susceptibility of Listeria monocytogenes Isolates from Invasive Infections in Poland (1997\u20132013). Sci. Rep., 8.","DOI":"10.1038\/s41598-018-32574-0"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2728","DOI":"10.1128\/AAC.01557-09","article-title":"Antimicrobial Resistance of Listeria monocytogenes Strains Isolated from Humans in France","volume":"54","author":"Morvan","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Rippa, A., Bilei, S., Peruzy, M.F., Marrocco, M.G., Leggeri, P., Boss\u00f9, T., and Murru, N. (2024). Antimicrobial Resistance of Listeria monocytogenes Strains Isolated in Food and Food-Processing Environments in Italy. Antibiotics, 13.","DOI":"10.3390\/antibiotics13060525"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"8110","DOI":"10.1021\/bi700625p","article-title":"Structure and Mechanism of the Genomically Encoded Fosfomycin Resistance Protein, FosX, from Listeria monocytogenes","volume":"46","author":"Fillgrove","year":"2007","journal-title":"Biochemistry"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1099\/jmm.0.46142-0","article-title":"Detection and Characterization of Tet(M) in Tetracycline-Resistant Listeria Strains from Human and Food-Processing Origins in Belgium and France","volume":"54","author":"Bertrand","year":"2005","journal-title":"J. Med. Microbiol."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Wi\u015bniewski, P., Zakrzewski, A.J., Zadernowska, A., and Chaj\u0119cka-Wierzchowska, W. (2022). Antimicrobial Resistance and Virulence Characterization of Listeria monocytogenes Strains Isolated from Food and Food Processing Environments. Pathogens, 11.","DOI":"10.3390\/pathogens11101099"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Roberts, M.C. (2011). Environmental Macrolide\u2013Lincosamide\u2013Streptogramin and Tetracycline Resistant Bacteria. Front. Microbio., 2.","DOI":"10.3389\/fmicb.2011.00040"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1128\/CMR.14.3.584-640.2001","article-title":"Listeria Pathogenesis and Molecular Virulence Determinants","volume":"14","author":"Kuhn","year":"2001","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Anwar, T.M., Pan, H., Chai, W., Ed-Dra, A., Fang, W., Li, Y., and Yue, M. (2022). Genetic Diversity, Virulence Factors, and Antimicrobial Resistance of Listeria monocytogenes from Food, Livestock, and Clinical Samples between 2002 and 2019 in China. Int. J. Food Microbiol., 366.","DOI":"10.1016\/j.ijfoodmicro.2022.109572"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.mib.2011.01.005","article-title":"Regulation of Listeria Virulence: PrfA Master and Commander","volume":"14","author":"Cain","year":"2011","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"e00060-19","DOI":"10.1128\/cmr.00060-19","article-title":"Human Listeriosis","volume":"36","author":"Koopmans","year":"2023","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/82_2016_30","article-title":"ActA of Listeria monocytogenes and Its Manifold Activities as an Important Listerial Virulence Factor","volume":"Volume 399","author":"Mannherz","year":"2016","journal-title":"The Actin Cytoskeleton and Bacterial Infection"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1038\/sj.emboj.7600750","article-title":"Gp96 Is a Receptor for a Novel Listeria monocytogenes Virulence Factor, Vip, a Surface Protein","volume":"24","author":"Cabanes","year":"2005","journal-title":"EMBO J."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.ijmm.2010.11.006","article-title":"Contribution of Listeria monocytogenes RecA to Acid and Bile Survival and Invasion of Human Intestinal Caco-2 Cells","volume":"301","author":"Abee","year":"2011","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1111\/mmi.14836","article-title":"Role of Internalin Proteins in the Pathogenesis of Listeria monocytogenes","volume":"116","author":"Ireton","year":"2021","journal-title":"Mol. Microbiol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1128\/IAI.71.3.1217-1224.2003","article-title":"Expression of Truncated Internalin A Is Involved in Impaired Internalization of Some Listeria monocytogenes Isolates Carried Asymptomatically by Humans","volume":"71","author":"Olier","year":"2003","journal-title":"Infect. Immun."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"1722","DOI":"10.1126\/science.1059852","article-title":"A Transgenic Model for Listeriosis: Role of Internalin in Crossing the Intestinal Barrier","volume":"292","author":"Lecuit","year":"2001","journal-title":"Science"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Pentecost, M., Otto, G., Theriot, J.A., and Amieva, M.R. (2006). Listeria monocytogenes Invades the Epithelial Junctions at Sites of Cell Extrusion. PLoS Pathog., 2.","DOI":"10.1371\/journal.ppat.0020003"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1046\/j.1365-2958.2000.01752.x","article-title":"CtsR Controls Class III Heat Shock Gene Expression in the Human Pathogen Listeria monocytogenes","volume":"35","author":"Nair","year":"2000","journal-title":"Mol. Microbiol."},{"key":"ref_88","doi-asserted-by":"crossref","unstructured":"Birk, M.S., Ahmed-Begrich, R., Tran, S., Elsholz, A.K.W., Frese, C.K., and Charpentier, E. (2021). Time-Resolved Proteome Analysis of Listeria monocytogenes during Infection Reveals the Role of the AAA+ Chaperone ClpC for Host Cell Adaptation. mSystems, 6.","DOI":"10.1128\/msystems.00215-21"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Alharbi, N.K., El-Hamid, M.I.A., Awad, N.F.S., El-Tarabili, R.M., Elazab, S.T., Mosbah, R.A., Elmanakhly, A.R., Alhomrani, M., Alamri, A.S., and Algarzae, N.K. (2025). Exploring the Dynamic Relationship between Antimicrobial Resistance, Virulence Fitness, and Host Responses in Listeria monocytogenes Infections. Sci. Rep., 15.","DOI":"10.1038\/s41598-025-10093-z"},{"key":"ref_90","first-page":"43","article-title":"Contribution of Sortase A to the Regulation of Listeria monocytogenes LPXTG Surface Proteins","volume":"15","author":"Mariscotti","year":"2012","journal-title":"Int. Microbiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1007\/s11274-008-9919-8","article-title":"A Study on the Effects of Some Laboratory-Derived Genetic Mutations on Biofilm Formation by Listeria monocytogenes","volume":"25","author":"Kumar","year":"2009","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.micres.2018.07.001","article-title":"An Essential Role for Hfq Involved in Biofilm Formation and Virulence in Serotype 4b Listeria monocytogenes","volume":"215","author":"Yao","year":"2018","journal-title":"Microbiol. Res."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/14\/21\/3788\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T05:18:57Z","timestamp":1762492737000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/14\/21\/3788"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,5]]},"references-count":92,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["foods14213788"],"URL":"https:\/\/doi.org\/10.3390\/foods14213788","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,5]]}}}