{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T06:26:11Z","timestamp":1772605571507,"version":"3.50.1"},"reference-count":69,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>To identify the underlying mechanisms by which H. rhamnoides fruit extract exerts regulatory effects on intestinal function, we investigated its chemical composition using UHPLC Q-Orbitrap HRMS and evaluated its biological effects on Aquaporin-3 (AQP-3) expression via Western blot in the intestinal epithelial cell line (HT-29). Moreover, fecal microbiota from healthy and constipated adults was employed to mimic the in vitro fermentation of the digested extract and evaluate its effects on gut microbiota functionality. Antioxidant capacity (i.e., Total Phenolic Contents (TPC), 2,2\u2032-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays) was assessed prior to and after simulated digestion and fermentation processes. Short-chain fatty acids (SCFAs) were quantified using UHPLC-RID of the fermented samples. In the extract, 23 compounds belonging to a variety of classes (mainly polyphenols) were tentatively identified. The extract significantly upregulated AQP-3 expression in the absence of cytotoxicity. After in vitro fermentation with gut microbiota isolated from constipated subjects, ABTS and FRAP values significantly decreased, as well as TPC, suggesting a greater consumption of antioxidant compounds, consistent with the increased production of radical compounds associated with constipation. Fermentation with intestinal microbiota with healthy and constipated gut microbiota resulted in an increase in SCFA. These results provide preliminary insights into a non-pharmacological strategy for functional constipation.<\/jats:p>","DOI":"10.3390\/foods14213800","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:14:45Z","timestamp":1762506885000},"page":"3800","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro Influence of a Chemically Characterized Hippophae rhamnoides L. Fruit Extract on Healthy and Constipated Human Gut Microbiota Functionality and Aquaporin-3 Expression"],"prefix":"10.3390","volume":"14","author":[{"given":"Lorenza Francesca","family":"De Lellis","sequence":"first","affiliation":[{"name":"Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-4348-9140","authenticated-orcid":false,"given":"\u00c1ngela","family":"Toledano-Mar\u00edn","sequence":"additional","affiliation":[{"name":"Departamento de Nutrici\u00f3n y Bromatolog\u00eda, Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos, Centro de Investigaci\u00f3n Biom\u00e9dica (CIBM), Universidad de Granada, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Navarro-Moreno","sequence":"additional","affiliation":[{"name":"Departamento de Nutrici\u00f3n y Bromatolog\u00eda, Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos, Centro de Investigaci\u00f3n Biom\u00e9dica (CIBM), Universidad de Granada, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5667-5176","authenticated-orcid":false,"given":"Elisabetta","family":"Caiazzo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2011-9752","authenticated-orcid":false,"given":"Gennaro","family":"Madonna","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Napoli Federico II, Via D. 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Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8365-9032","authenticated-orcid":false,"given":"Luana","family":"Izzo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8502-7186","authenticated-orcid":false,"given":"Antonio","family":"Paolillo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8084-9976","authenticated-orcid":false,"given":"Alessandro","family":"Di Minno","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hammad","family":"Ullah","sequence":"additional","affiliation":[{"name":"School of Pharmacy, University of Management and Technology, Lahore 54000, Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0810-8460","authenticated-orcid":false,"given":"Maria Vittoria","family":"Morone","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0395-7962","authenticated-orcid":false,"given":"Anna","family":"De Filippis","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1576-6290","authenticated-orcid":false,"given":"Massimiliano","family":"Galdiero","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania \u201cL. Vanvitelli\u201d, 80138 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armando","family":"Ialenti","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Napoli Federico II, Via D. Montesano 49, 80131 Naples, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1428-4353","authenticated-orcid":false,"given":"Jos\u00e9 \u00c1ngel","family":"Rufi\u00e1n Henares","sequence":"additional","affiliation":[{"name":"Departamento de Nutrici\u00f3n y Bromatolog\u00eda, Instituto de Nutrici\u00f3n y Tecnolog\u00eda de los Alimentos, Centro de Investigaci\u00f3n Biom\u00e9dica (CIBM), Universidad de Granada, 18071 Granada, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4870-7713","authenticated-orcid":false,"given":"Maria","family":"Daglia","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Napoli Federico II, Via D. 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Hepatol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1159\/000517212","article-title":"Functional constipation in older adults: Prevalence, clinical symptoms and subtypes, association with frailty, and impact on quality of life","volume":"68","author":"Arco","year":"2022","journal-title":"Gerontology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1582","DOI":"10.1038\/ajg.2011.164","article-title":"Prevalence of, and risk factors for, chronic idiopathic constipation in the community: Systematic review and meta-analysis","volume":"106","author":"Suares","year":"2011","journal-title":"Am. J. Gastroenterol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"92","DOI":"10.4166\/kjg.2019.73.2.92","article-title":"Chronic functional constipation","volume":"73","author":"Shin","year":"2019","journal-title":"Korean J. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1111\/apt.13167","article-title":"Systematic review: Dietary fibre and FODMAP-restricted diet in the management of constipation and irritable bowel syndrome","volume":"41","author":"Rao","year":"2015","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1111\/j.1365-2036.2011.04602.x","article-title":"Systematic review: The effects of fibre in the management of chronic idiopathic constipation","volume":"33","author":"Suares","year":"2011","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1111\/j.1365-2036.1995.tb00433.x","article-title":"Effects of psyllium therapy on stool characteristics, colon transit and anorectal function in chronic idiopathic constipation","volume":"9","author":"Ashraf","year":"1995","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1007\/s40265-020-01305-z","article-title":"Functional constipation: Individualising assessment and treatment","volume":"80","author":"Pannemans","year":"2020","journal-title":"Drugs"},{"key":"ref_9","first-page":"nuae047","article-title":"The effectiveness and safety of natural food and food-derived extract supplements for treating functional gastrointestinal disorders\u2014Current perspectives","volume":"83","author":"Liu","year":"2024","journal-title":"Nutr. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS), Younes, M., Aggett, P., Aguilar, F., Crebelli, R., Filipi\u010d, M., Frutos, M.J., Galtier, P., Gott, D., and Gundert-Remy, U. (2018). Safety of hydroxyanthracene derivatives for use in food. EFSA J., 16, e05090.","DOI":"10.2903\/j.efsa.2018.5090"},{"key":"ref_11","first-page":"1128519","article-title":"Bioactive profile, health benefits and safety evaluation of sea buckthorn (Hippophae rhamnoides L.): A review","volume":"2","author":"Wani","year":"2016","journal-title":"Cogent Food Agric."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"4769","DOI":"10.1021\/acs.jafc.2c06916","article-title":"Hippophae rhamnoides L.: A comprehensive review on the botany, traditional uses, phytonutrients, health benefits, quality markers, and applications","volume":"71","author":"Ma","year":"2023","journal-title":"J. Agric. Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.jep.2011.09.024","article-title":"Medicinal and therapeutic potential of Sea buckthorn (Hippophae rhamnoides L.)","volume":"138","author":"Suryakumar","year":"2011","journal-title":"J. Ethnopharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Wang, Z., Zhao, F., Wei, P., Chai, X., Hou, G., and Meng, Q. (2022). Phytochemistry, health benefits, and food applications of sea buckthorn (Hippophae rhamnoides L.): A comprehensive review. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1036295"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"G\u00e2tlan, A.M., and Gutt, G. (2021). Sea buckthorn in plant based diets. An analytical approach of sea buckthorn fruits composition: Nutritional value, applications, and health benefits. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18178986"},{"key":"ref_16","first-page":"2527","article-title":"Evaluation of prokinetic and laxative effects of Hippophae rhamnoides in rodents","volume":"32","author":"Hanif","year":"2019","journal-title":"Pak. J. Pharm. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Izzo, L., Castaldo, L., Lombardi, S., Gaspari, A., Grosso, M., and Ritieni, A. (2022). Bioaccessibility and antioxidant capacity of bioactive compounds from various typologies of canned tomatoes. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.849163"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1039\/C3FO60702J","article-title":"A standardised static in vitro digestion method suitable for food\u2014An international consensus","volume":"5","author":"Minekus","year":"2014","journal-title":"Food Funct."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Buccato, D.G., Delgado-Osorio, A., De Lellis, L.F., Morone, M.V., Ullah, H., Izzo, L., Lombardi, S., Di Minno, A., Riccioni, C.V., and Moriki, D. (2025). Exploring the influence of a pomegranate extract on the functionality of healthy and diseased human gut microbiota: An in vitro study. Molecules, 30.","DOI":"10.3390\/molecules30071634"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3186","DOI":"10.1038\/s41596-021-00537-x","article-title":"An in vitro batch fermentation protocol for studying the contribution of food to gut microbiota composition and functionality","volume":"16","author":"Molino","year":"2021","journal-title":"Nat. Protoc."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.jfca.2014.10.001","article-title":"Phenolic compounds and antioxidant activity of Spanish commercial grape juices","volume":"38","year":"2015","journal-title":"J. Food Compos. Anal."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1021\/jf00049a007","article-title":"Antioxidant activity of various tea extracts in relation to their antimutagenicity","volume":"43","author":"Yen","year":"1995","journal-title":"J. Agric. Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1016\/S0891-5849(98)00315-3","article-title":"Antioxidant activity applying an improved ABTS radical cation decolorization assay","volume":"26","author":"Re","year":"1999","journal-title":"Free Radic. Biol. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1006\/abio.1996.0292","article-title":"The ferric reducing ability of plasma (FRAP) as a measure of \u201cantioxidant power\u201d: The FRAP assay","volume":"239","author":"Benzie","year":"1996","journal-title":"Anal. Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"6452","DOI":"10.1021\/acs.jafc.7b02302","article-title":"High Antioxidant action and prebiotic activity of hydrolyzed spent coffee grounds (HSCG) in a simulated digestion\u2013fermentation model: Toward the development of a novel food supplement","volume":"65","author":"Panzella","year":"2017","journal-title":"J. Agric. Food Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.foodchem.2015.12.019","article-title":"Antioxidant capacity, total phenols and color profile during the storage of selected plants used for infusion","volume":"199","year":"2016","journal-title":"Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2709","DOI":"10.3748\/wjg.v30.i20.2709","article-title":"Hydrogen-rich water alleviates constipation by attenuating oxidative stress through the sirtuin1\/nuclear factor-erythroid-2-related factor 2\/heme oxygenase-1 signaling pathway","volume":"30","author":"Chen","year":"2024","journal-title":"World J. Gastroenterol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1093\/ajcn\/79.5.727","article-title":"Polyphenols: Food sources and bioavailability","volume":"79","author":"Manach","year":"2004","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_29","first-page":"5","article-title":"Food-based strategies to modulate the composition of the intestinal microbiota and their associated health effects","volume":"60","author":"Bosscher","year":"2009","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.phrs.2009.11.001","article-title":"Interactions of gut microbiota with functional food components and nutraceuticals","volume":"61","author":"Laparra","year":"2010","journal-title":"Pharmacol Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Pan, R., Wang, L., Xu, X., Chen, Y., Wang, H., Wang, G., Zhao, J., and Chen, W. (2022). Crosstalk between the Gut Microbiome and Colonic Motility in Chronic Constipation: Potential Mechanisms and Microbiota Modulation. Nutrients, 14.","DOI":"10.3390\/nu14183704"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Li, Y., Li, P., Yang, K., He, Q., Wang, Y., Sun, Y., He, C., and Xiao, P. (2021). Impact of drying methods on phenolic components and antioxidant activity of sea buckthorn (Hippophae rhamnoides L.) berries from different varieties in China. Molecules, 26.","DOI":"10.3390\/molecules26237189"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/j.foodchem.2017.04.107","article-title":"Phytochemicals content and antioxidant properties of sea buckthorn (Hippophae rhamnoides L.) as affected by heat treatment\u2013Quantitative spectroscopic and kinetic approaches","volume":"233","author":"Ursache","year":"2017","journal-title":"Food Chem."},{"key":"ref_34","first-page":"411","article-title":"Content of phenolic compounds in various sea buckthorn parts","volume":"67","author":"Sne","year":"2013","journal-title":"Proc. Latv. Acad. Sci. Sect. B"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"79","DOI":"10.5530\/ax.2012.4.14","article-title":"Antioxidant potential and total phenolic contents of seabuckthorn (Hippophae rhamnoides) pomace","volume":"2","author":"Kant","year":"2012","journal-title":"Free. Radic. Antioxid."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1002\/fsn3.3155","article-title":"Phenolic compounds, antioxidant activity and sensory evaluation of sea buckthorn (Hippophae rhamnoides L.) leaf tea","volume":"11","author":"He","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Martinez-Gonzalez, A.I., D\u00edaz-S\u00e1nchez, \u00c1.G., de la Rosa, L.A., Vargas-Requena, C.L., Bustos-Jaimes, I., and Alvarez-Parrilla, E. (2017). Polyphenolic compounds and digestive enzymes: In vitro non-covalent interactions. Molecules, 22.","DOI":"10.3390\/molecules22040669"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.foodchem.2006.06.025","article-title":"Stability of polyphenols in chokeberry (Aronia melanocarpa) subjected to in vitro gastric and pancreatic digestion","volume":"102","year":"2007","journal-title":"Food Chem."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"110552","DOI":"10.1016\/j.lwt.2020.110552","article-title":"Stability and mechanism of phenolic compounds from raspberry extract under in vitro gastrointestinal digestion","volume":"139","author":"Hao","year":"2021","journal-title":"LWT"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1002\/mnfr.201100566","article-title":"Gastrointestinal stability and bioavailability of (poly) phenolic compounds following ingestion of Concord grape juice by humans","volume":"56","author":"Stalmach","year":"2012","journal-title":"Mol. Nutr. Food Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1083","DOI":"10.1016\/j.foodchem.2010.03.079","article-title":"Polyphenol and nutrient release from skin of almonds during simulated human digestion","volume":"122","author":"Mandalari","year":"2010","journal-title":"Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Xie, L., Deng, Z., Zhang, J., Dong, H., Wang, W., Xing, B., and Liu, X. (2022). Comparison of flavonoid O-glycoside, C-glycoside and their aglycones on antioxidant capacity and metabolism during in vitro digestion and in vivo. Foods, 11.","DOI":"10.3390\/foods11060882"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1179\/135100002125000334","article-title":"On-line antioxidant activity determination: Comparison of hydrophilic and lipophilic antioxidant activity using the ABTS\u2022+ assay","volume":"7","author":"Cano","year":"2002","journal-title":"Redox Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s11101-016-9459-z","article-title":"The chemistry of gut microbial metabolism of polyphenols","volume":"15","author":"Stevens","year":"2016","journal-title":"Phytochem. Rev."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.anaerobe.2013.07.009","article-title":"Fecal microbial metabolism of polyphenols and its effects on human gut microbiota","volume":"23","author":"Parkar","year":"2013","journal-title":"Anaerobe"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1136\/gut.34.4.437","article-title":"Sulphate reducing bacteria and hydrogen metabolism in the human large intestine","volume":"34","author":"Gibson","year":"1993","journal-title":"Gut"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/MMBR.00092-19","article-title":"Molecular hydrogen metabolism: A widespread trait of pathogenic bacteria and protists","volume":"84","author":"Benoit","year":"2020","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Abudureheman, B., Yu, X., Fang, D., and Zhang, H. (2022). Enzymatic oxidation of tea catechins and its mechanism. Molecules, 27.","DOI":"10.3390\/molecules27030942"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Di Pede, G., Bresciani, L., Calani, L., Petrangolini, G., Riva, A., Allegrini, P., Del Rio, D., and Mena, P. (2020). The human microbial metabolism of quercetin in different formulations: An in vitro evaluation. Foods, 9.","DOI":"10.3390\/foods9081121"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1332","DOI":"10.1016\/j.cell.2016.05.041","article-title":"From dietary fiber to host physiology: Short-chain fatty acids as key bacterial metabolites","volume":"165","author":"Koh","year":"2016","journal-title":"Cell"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Prasad, K.N., and Bondy, S.C. (2019). Dietary fibers and their fermented short-chain fatty acids in prevention of human diseases. Bioact. Carbohydr. Diet. Fibre, 17.","DOI":"10.1016\/j.bcdf.2018.09.001"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.tibs.2014.02.005","article-title":"How do gut microbes break down dietary fiber?","volume":"39","author":"Terrapon","year":"2014","journal-title":"Trends Biochem. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1519","DOI":"10.3748\/wjg.v17.i12.1519","article-title":"Potential beneficial effects of butyrate in intestinal and extraintestinal diseases","volume":"17","author":"Canani","year":"2011","journal-title":"World J. Gastroenterol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"103953","DOI":"10.1016\/j.jff.2020.103953","article-title":"Acetic acid and butyric acid released in large intestine play different roles in the alleviation of constipation","volume":"69","author":"Wang","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S1098-612X(03)00002-0","article-title":"Short chain fatty acids stimulate feline colonic smooth muscle contraction","volume":"5","author":"Rondeau","year":"2003","journal-title":"J. Feline Med. Surg."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"R1269","DOI":"10.1152\/ajpregu.00442.2002","article-title":"Short-chain fatty acids stimulate colonic transit via intraluminal 5-HT release in rats","volume":"284","author":"Fukumoto","year":"2003","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"5543","DOI":"10.3748\/wjg.v25.i36.5543","article-title":"Evaluation and comparison of short chain fatty acids composition in gut diseases","volume":"25","author":"Niccolai","year":"2019","journal-title":"World J. Gastroenterol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1093\/gastro\/goab035","article-title":"Role of gut microbiota in functional constipation","volume":"9","author":"Zhang","year":"2021","journal-title":"Gastroenterol. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Guzel, T., and Mirowska-Guzel, D. (2022). The Role of Serotonin Neurotransmission in Gastrointestinal Tract and Pharmacotherapy. Molecules, 27.","DOI":"10.3390\/molecules27051680"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1017\/S0029665119000934","article-title":"Probiotics and constipation: Mechanisms of action, evidence for effectiveness and utilisation by patients and healthcare professionals","volume":"79","author":"Dimidi","year":"2020","journal-title":"Proc. Nutr. Soc."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1111\/j.1651-2227.1998.tb01547.x","article-title":"The intestinal ecosystem in chronic functional constipation","volume":"87","author":"Zoppi","year":"1998","journal-title":"Acta Paediatr."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1016\/j.dld.2005.06.008","article-title":"Alterations in the colonic flora and intestinal permeability and evidence of immune activation in chronic constipation","volume":"37","author":"Khalif","year":"2005","journal-title":"Dig. Liver Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"111","DOI":"10.5056\/jnm14048","article-title":"Change of fecal flora and effectiveness of the short-term VSL#3 probiotic treatment in patients with functional constipation","volume":"21","author":"Kim","year":"2015","journal-title":"J. Neurogastroenterol. Motil."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Mancabelli, L., Milani, C., Lugli, G.A., Turroni, F., Mangifesta, M., Viappiani, A., Ticinesi, A., Nouvenne, A., Meschi, T., and van Sinderen, D. (2017). Unveiling the gut microbiota composition and functionality associated with constipation through metagenomic analyses. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-10663-w"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1079\/BJN20041150","article-title":"Contribution of acetate to butyrate formation by human faecal bacteria","volume":"9","author":"Duncan","year":"2004","journal-title":"Br. J. Nutr."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Ikarashi, N., Kon, R., and Sugiyama, K. (2016). Aquaporins in the colon as a new therapeutic target in diarrhea and constipation. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17071172"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Zhang, H., Zu, Q., Zhang, J., Liu, S., Zhang, G., Chang, X., and Li, X. (2024). Soluble dietary fiber of hawthorn relieves constipation induced by loperamide hydrochloride by improving intestinal flora and inflammation, thereby regulating the aquaporin ion pathway in mice. Foods, 13.","DOI":"10.3390\/foods13142220"},{"key":"ref_68","first-page":"649","article-title":"Naringenin induces laxative effects by upregulating the expression levels of c-Kit and SCF, as well as those of aquaporin 3 in mice with loperamide-induced constipation","volume":"41","author":"Yin","year":"2018","journal-title":"Int. J. Mol. Med."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"106444","DOI":"10.1016\/j.jff.2024.106444","article-title":"Improving intestinal health and mitigating Loperamide-Induced constipation through the modulation of Aquaporin-3 expression, reduction of oxidative stress, and suppression of inflammatory response by fermented rice extract","volume":"121","author":"Lee","year":"2024","journal-title":"J. Funct. 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