{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:43:16Z","timestamp":1787028196861,"version":"build-2736575974"},"reference-count":94,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Ochratoxin A (OTA), Zearalenone (ZEA), and Fumonisins (FB) are common contaminants of poultry feed associated with oxidative damage and potentially dangerous residues in products from exposed animals. We investigated the molecular effects in broilers of a short-term (10 days) dietary exposure to OTA (0.26 mg\/kg), ZEA (2.9 mg\/kg), or FB (60 mg\/kg) on cytochrome P450 enzymes (CYP), drug transporters (DT) and the antioxidant defence system. OTA markedly decreased serum antioxidant capacity, while all mycotoxins depressed reduced glutathione content and increased lipid peroxidation in the liver, indicating a hepatic pro-oxidant effect. All the tested mycotoxins also reduced both the activities and the gene expression of selected antioxidant enzymes in the liver and duodenum as a result of the modulation of the Nrf2\/Keap1 pathway. Moreover, mycotoxins differentially altered the hepatic and intestinal gene expression of CYP enzymes (i.e., CYP2A6, CYP2C45, CYP3A4, and CYP1A isoforms). Finally, the transcription of selected DT (i.e., ABCB1, ABCC2 and ABCG2) was generally enhanced in both the liver and duodenum. In conclusion, short-term exposure to OTA, ZEA, or FB at dietary concentrations higher than those recommended in the EU, but occurring in third countries, not only disrupt the antioxidant defence but also affect the expression of CYP and DT, which might potentially alter the kinetics of drugs and toxicants. Our results provide new insights into mycotoxin adverse effects in the light to assess the effectiveness of new mitigation strategies that contribute to food and feed safety.<\/jats:p>","DOI":"10.3390\/foods14244249","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T14:22:40Z","timestamp":1765376560000},"page":"4249","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Short-Term Dietary Exposure to Ochratoxin A, Zearalenone or Fumonisins in Broiler Chickens: Effects on Cytochrome P450 Enzymes, Drug Transporters and Antioxidant Defence Systems"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4353-2963","authenticated-orcid":false,"given":"Neenu","family":"Amminikutty","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8431-0695","authenticated-orcid":false,"given":"Matteo","family":"Cuccato","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8274-442X","authenticated-orcid":false,"given":"Watanya","family":"Jarriyawattanachaikul","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Gariglio","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8681-1263","authenticated-orcid":false,"given":"Donato","family":"Greco","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, Italian National Research Council, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7344-9712","authenticated-orcid":false,"given":"Vito","family":"D\u2019Ascanio","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, Italian National Research Council, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"Avantaggiato","sequence":"additional","affiliation":[{"name":"Institute of Sciences of Food Production, Italian National Research Council, 70126 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8011-6999","authenticated-orcid":false,"given":"Achille","family":"Schiavone","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0624-7787","authenticated-orcid":false,"given":"Carlo","family":"Nebbia","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9005-5301","authenticated-orcid":false,"given":"Flavia","family":"Girolami","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, University of Turin, 10095 Grugliasco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hafez, H.M., and Attia, Y.A. 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