{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T21:01:40Z","timestamp":1789765300832,"version":"4.0.1"},"reference-count":33,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"DIB of Universidad Nacional de Colombia and the Direcci\u00f3n General de Investigaciones of Universidad Santiago de Cali"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Isabella grape pomace (IGP) the primary by-product of Colombia\u2019s winemaking industry, represents a promising source of nutraceuticals with potential uses in the food industry. This study developed a sequential green extraction process to recover nutraceutical from IGP. The approach integrated supercritical fluid extraction with CO2 (SFE-CO2) to obtain lipophilic compounds, followed by SFE with ethanol\/water as co-solvent (SFE-CO2\/EtOH:H2O) for medium-polarity phenolics, and pressurized liquid extraction (PLE) with EtOH:H2O to recover the polar phenolic-rich fraction. The extraction parameters were optimized using response surface methodology, and optimal conditions were identified: SFE-CO2 at 31.7 MPa\/58.9 \u00b0C yielded 6.95% extract rich in linoleic acid (65.5%) and \u03b1-tocopherol (107.2 mg\/kg); SFE-CO2\/EtOH:H2O with 15% of co-solvent produced extracts with high phenolic content (105.35 mg GAE\/g) and antioxidant activity (0.18 mmol TE\/g); while PLE at 58.91% of EtOH\/107.98 \u00b0C achieved notable recovery of flavonoids (757.18 mg QE\/g), anthocyanins(1508 \u03bcg MAE\/g) and condensed tannins (258.39 mg ECE\/g), with potent antioxidant capacity (130.40 mmol TE\/g). The sequential process demonstrated synergistic effects, with a total cumulative yield of 41.08% and phenolic recovery of 349.89 mg GAE\/g extract. This approach offers a sustainable biorefinery approach for transforming IGP into high-value nutraceutical ingredients.<\/jats:p>","DOI":"10.3390\/foods15010054","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T11:22:14Z","timestamp":1766575334000},"page":"54","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Valorization of Isabella Grape (Vitis labrusca L.) Pomace Through the Recovery of Nutraceuticals by Sequential Green Extraction Technologies"],"prefix":"10.3390","volume":"15","author":[{"given":"Jhonattan S\u00e1nchez","family":"S\u00e1nchez","sequence":"first","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Qu\u00edmica de Alimentos (GIQA), Departamento de Qu\u00edmica, Facultad de Ciencias, Universidad Nacional de Colombia, Carrera 30 No 45-03, Bogot\u00e1 110911, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0896-4114","authenticated-orcid":false,"given":"Fabi\u00e1n","family":"Parada-Alfonso","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Qu\u00edmica de Alimentos (GIQA), Departamento de Qu\u00edmica, Facultad de Ciencias, Universidad Nacional de Colombia, Carrera 30 No 45-03, Bogot\u00e1 110911, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8106-7212","authenticated-orcid":false,"given":"Henry I.","family":"Castro-Vargas","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Electroqu\u00edmica y Medio Ambiente (GIEMA\/CICBA), Departamento de Ciencias Naturales, Extractas y Estad\u00edstica, Facultad de Ciencias, Universidad Santiago de Cali, Campus Pampalinda, Calle 5 No 62-00, Santiago de Cali 760035, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"ref_1","unstructured":"Instituto Colombiano Agropecuario (ICA) (2012). 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