{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T03:19:28Z","timestamp":1770520768548,"version":"3.49.0"},"reference-count":54,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T00:00:00Z","timestamp":1770249600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>A rapid and simplified LC\u2013MS method was developed for quantifying vitamins D3 (cholecalciferol) and K2 (menaquinone-4 and menaquinone-7) in high-fat chicken meat products. Sample preparation involves a two-step solvent extraction followed by centrifugation. Efficient separation was achieved on a Gemini C18 column, and electrospray in positive mode was used for detection. Method validation confirmed good performance and reproducibility. The method was successfully applied to both fortified and unfortified chicken p\u00e2t\u00e9 samples. Owing to its simplicity, robustness, and sensitivity, this approach provides a practical and reliable means for quantifying fat-soluble vitamins in complex animal-derived matrices and can serve as a foundation for broader applications in high-fat food products.<\/jats:p>","DOI":"10.3390\/foods15030570","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T11:13:01Z","timestamp":1770289981000},"page":"570","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simple, Sensitive and Simultaneous Determination of Free D3 and K2 Vitamins in Fortified Chicken Meat Products by LC-MS\/MS with Electrospray Ionisation"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3859-7338","authenticated-orcid":false,"given":"Mitja","family":"Kri\u017eman","sequence":"first","affiliation":[{"name":"Laboratory for Food Chemistry, Department of Analytical Chemistry, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hubicka, U., Padiasek, A., \u017buromska Witek, B., and Szl\u00f3sarczyk, M. (2020). Determination of Vitamins K1, K2 MK 4, MK 7, MK 9 and D3 in Pharmaceutical Products and Dietary Supplements by TLC Densitometry. Processes, 8.","DOI":"10.3390\/pr8070870"},{"key":"ref_2","first-page":"7454376","article-title":"The Synergistic Interplay between Vitamins D and K for Bone and Cardiovascular Health: A Narrative Review","volume":"2017","author":"Pilz","year":"2017","journal-title":"Int. J. Endocrinol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1002\/fsn3.3213","article-title":"The biological responses of vitamin K2: A comprehensive review","volume":"11","author":"Yan","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e210594","DOI":"10.1530\/EC-21-0594","article-title":"100 YEARS OF VITAMIN D: Historical aspects of vitamin D","volume":"11","author":"Jones","year":"2022","journal-title":"Endocr. Connect."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Aaseth, J.O., Finnes, T.E., Askim, M., and Alexander, J. (2024). The Importance of Vitamin K and the Combination of Vitamins K and D for Calcium Metabolism and Bone Health: A Review. Nutrients, 16.","DOI":"10.20944\/preprints202406.0766.v1"},{"key":"ref_6","first-page":"1","article-title":"Biofortification of meat with vitamin D","volume":"13","author":"Duffy","year":"2019","journal-title":"CAB Rev. Perspect. Agric. Vet. Sci. Nutr. Nat. Resour."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2622","DOI":"10.1093\/jn\/nxab180","article-title":"Vitamin D Food Fortification and Biofortification Increases Serum 25-Hydroxyvitamin D Concentrations in Adults and Children: An Updated and Extended Systematic Review and Meta-Analysis of Randomized Controlled Trials","volume":"151","author":"Dunlop","year":"2021","journal-title":"J. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1308","DOI":"10.1002\/jps.2600570806","article-title":"Thin-Layer Chromatographic Assay of Vitamin D in High-Potency Preparations: Analysis of Fat-Soluble Vitamins IX","volume":"57","author":"Hanewald","year":"1968","journal-title":"J. Pharm. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3177\/jnsv.22.41","article-title":"Gas-liquid chromatographic determination of vitamin D in multivitamin preparation","volume":"22","author":"Kobayashi","year":"1976","journal-title":"J. Nutr. Sci. Vitaminol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/0039-128X(82)90063-0","article-title":"Cholecalciferol sulfate identification in human milk by HPLC","volume":"39","author":"Boulch","year":"1982","journal-title":"Steroids"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0076-6879(86)23020-7","article-title":"Quantitation of vitamin D2, vitamin D3, 25-hydroxyvitamin D2, and 25-hydroxyvitamin D3 in human milk","volume":"123","author":"Hollis","year":"1986","journal-title":"Methods Enzymol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"xv","DOI":"10.1016\/S0315-5463(83)72137-1","article-title":"A simple HPLC analysis of vitamin D in fortified milk","volume":"16","author":"Jang","year":"1983","journal-title":"Can. Inst. Food Technol. J."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0160-5402(89)90062-4","article-title":"Simplified HPLC method for quantitation of vitamin D in blood serum","volume":"21","author":"Lukaszkiewicz","year":"1989","journal-title":"J. Pharmacol. Methods"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1093\/ajcn\/51.5.846","article-title":"Vitamin K concentrations in the plasma and liver of surgical patients","volume":"51","author":"Usui","year":"1990","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1007\/BF02466663","article-title":"HPLC analysis of fat-soluble vitamins on standard and narrow bore columns with UV, electrochemical and particle beam MS detection","volume":"44","author":"Andreoli","year":"1997","journal-title":"Chromatographia"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"768","DOI":"10.1007\/BF00323634","article-title":"Identification of vitamins A, D and E by particle beam liquid chromatography-mass spectrometry","volume":"351","author":"Careri","year":"1995","journal-title":"Fresenius J. Anal. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.chroma.2003.09.062","article-title":"Simultaneous quantification of vitamins A, D3 and E in fortified infant formulae by liquid chromatography-mass spectrometry","volume":"1022","author":"Heudi","year":"2004","journal-title":"J. Chromatogr. A"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1021\/ac0489667","article-title":"Method for the determination of vitamin K homologues in human plasma using high-performance liquid chromatography-tandem mass spectrometry","volume":"77","author":"Suhara","year":"2005","journal-title":"Anal. Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1002\/bmc.1130050404","article-title":"Analysis of vitamin D and its metabolites using thermospray liquid chromatography\/mass spectrometry","volume":"5","author":"Watson","year":"1991","journal-title":"Biomed. Chromatogr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.jfca.2010.08.002","article-title":"Determination of vitamin D3 and 25-hydroxyvitamin D3 in foodstuffs by HPLC UV-DAD and LC\u2013MS\/MS","volume":"24","author":"Bilodeau","year":"2011","journal-title":"J. Food Compos. Anal."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"129588","DOI":"10.1016\/j.foodchem.2021.129588","article-title":"Quantification of vitamin D3 and 25-hydroxyvitamin D3 in food\u2014The impact of eluent additives and labelled internal standards on matrix effects in LC-MS\/MS analysis","volume":"357","author":"Barnkob","year":"2021","journal-title":"Food Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1042","DOI":"10.1016\/j.foodchem.2012.08.041","article-title":"Vitamin D and 25-hydroxyvitamin D determination in meats by LC-IT-MS","volume":"138","author":"Strobel","year":"2013","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.trac.2016.12.001","article-title":"Advanced analytical techniques for fat soluble vitamin analysis","volume":"87","author":"Fanali","year":"2017","journal-title":"Trends Anal. Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4613","DOI":"10.1007\/s00216-022-04097-1","article-title":"Sample preparation techniques for extraction of vitamin D metabolites from non conventional biological sample matrices prior to LC\u2013MS\/MS analysis","volume":"414","author":"Alexandridou","year":"2022","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/j.chembiol.2013.12.016","article-title":"Vitamin D metabolism, mechanism of action, and clinical applications","volume":"21","author":"Bikle","year":"2014","journal-title":"Chem. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"106633","DOI":"10.1016\/j.jsbmb.2024.106633","article-title":"The validation of an LC\u2013MS\/MS method for the quantification of vitamin D metabolites in human milk and their biological variability in Gambian women","volume":"245","author":"Jones","year":"2025","journal-title":"J. Steroid Biochem. Mol. Biol."},{"key":"ref_27","first-page":"1","article-title":"Rapid and simultaneous determination of 25-OH-vitamin D2 and D3 in human serum by LC\/MS\/MS: Validation and uncertainty assessment","volume":"1","author":"Bilsel","year":"2007","journal-title":"J. Chem. Metrl"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.jchromb.2016.01.049","article-title":"High throughput LC\u2013MS\/MS method for the simultaneous analysis of multiple vitamin D analytes in serum","volume":"1014","author":"Jenkinson","year":"2016","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"113529","DOI":"10.1016\/j.jpba.2020.113529","article-title":"Improved sample preparation method for fast LC\u2013MS\/MS analysis of vitamin D metabolites in serum","volume":"190","author":"Rola","year":"2020","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3065","DOI":"10.1021\/acs.analchem.1c04016","article-title":"Simultaneous Determination of Vitamin D and Its Hydroxylated and Esterified Metabolites by Ultrahigh-Performance Supercritical Fluid Chromatography-Tandem Mass Spectrometry","volume":"94","author":"Sandahl","year":"2022","journal-title":"Anal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"115522","DOI":"10.1016\/j.jpba.2023.115522","article-title":"Improved quantitative LC\u2013MS\/MS analysis of vitamin D metabolites in serum after one-pot double derivatization","volume":"234","author":"Schorr","year":"2023","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1093\/nutrit\/nuab061","article-title":"Vitamin K\u2014Sources, physiological role, kinetics, deficiency, detection, therapeutic use, and toxicity","volume":"80","author":"Carazo","year":"2022","journal-title":"Nutr. Rev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1160\/TH08-03-0147","article-title":"Metabolism and cell biology of vitamin K","volume":"100","author":"Shearer","year":"2008","journal-title":"Thromb. Haemost."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1007\/s12161-024-02643-4","article-title":"Development of vitamin K analysis method using column switching high-performance liquid chromatography method and analysis results of various food items for vitamin K content","volume":"17","author":"Tanaka","year":"2024","journal-title":"Food Anal. Methods"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"464548","DOI":"10.1016\/j.chroma.2023.464548","article-title":"Analysis of vitamin K1 and major K2 variants in rat\/human serum and lipoprotein fractions by a rapid, simple, and sensitive UHPLC-MS\/MS method","volume":"1714","author":"Carazo","year":"2024","journal-title":"J. Chromatogr. A"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"133009","DOI":"10.1016\/j.foodchem.2022.133009","article-title":"Novel QuEChERS-ultra-performance liquid chromatography-atmospheric-pressure chemical ionization tandem mass spectrometry method for the simultaneous determination of vitamin D and vitamin K in vitamin-fortified nanoemulsions","volume":"389","author":"Kim","year":"2022","journal-title":"Food Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1007\/s007760070003","article-title":"Effect of combined administration of vitamin D3 and vitamin K2 on bone mineral density of the lumbar spine in postmenopausal women with osteoporosis","volume":"5","author":"Iwamoto","year":"2000","journal-title":"J. Orthop. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1017\/S0029665121003803","article-title":"Using food fortification to improve vitamin D bioaccessibility and intakes","volume":"81","author":"McCourt","year":"2022","journal-title":"Proc. Nutr. Soc."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.jfca.2018.09.008","article-title":"Survey of vitamin D and 25-hydroxyvitamin D in traditional native Alaskan meats, fish, and oils","volume":"74","author":"Phillips","year":"2018","journal-title":"J. Food Compos. Anal."},{"key":"ref_40","first-page":"e10453","article-title":"Natural Vitamin D in Food: To What Degree Does 25-Hydroxyvitamin D Contribute to the Vitamin D Activity in Food?","volume":"5","author":"Jakobsen","year":"2021","journal-title":"J. Bone Miner. Res. Plus"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2394","DOI":"10.1021\/jf00057a015","article-title":"Contents of cholecalciferol, ergocalciferol, and their 25-hydroxylated metabolites in milk products and raw meat and liver as determined by HPLC","volume":"43","author":"Mattila","year":"1995","journal-title":"J. Agric. Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"8298","DOI":"10.1021\/jf2012634","article-title":"Effect of different vitamin D supplementations in poultry feed on vitamin D content of eggs and chicken meat","volume":"59","author":"Mattila","year":"2011","journal-title":"J. Agric. Food Chem."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/j.chroma.2008.12.023","article-title":"Comparison of electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization for determining estrogenic chemicals in water by liquid chromatography tandem mass spectrometry with chemical derivatizations","volume":"1216","author":"Lien","year":"2009","journal-title":"J. Chromatogr. A"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.jpha.2016.03.006","article-title":"Comparison of ESI- and APCI-LC-MS\/MS methods: A case study of levonorgestrel in human plasma","volume":"6","author":"Wang","year":"2016","journal-title":"J. Pharm. Anal."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4905","DOI":"10.1007\/s00216-018-1137-0","article-title":"LC\/MS analysis of vitamin D metabolites by dielectric barrier discharge ionization and a comparison with electrospray ionization and atmospheric pressure chemical ionization","volume":"410","author":"Hagenhoff","year":"2018","journal-title":"Anal. Bioanal. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1002\/oms.1210100205","article-title":"Contrasting reactions for conversion of some dihydroaromatic cations to aromatic cations through hydrogen elimination","volume":"10","author":"Brady","year":"1975","journal-title":"Org. Mass. Spectrom."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"5259","DOI":"10.1021\/ja00384a001","article-title":"Structure and isomerization of C7H7+ ions formed in the charge-transfer-induced fragmentation of ethylbenzene, toluene, and norbornadiene","volume":"104","author":"Ausloos","year":"1982","journal-title":"J. Am. Chem. Soc."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1002\/oms.1210181105","article-title":"The structure of decomposing [C7H7O]+ ions: Benzyl versus tropylium ion structures","volume":"18","author":"Russell","year":"1983","journal-title":"Org. Mass. Spectrom."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0301-0104(00)00174-9","article-title":"Photoionization mass spectrometry of six isomers of C7H8 in the 7\u201322 eV photon energy range","volume":"260","author":"Schwell","year":"2000","journal-title":"Chem. Phys."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.comptc.2016.07.018","article-title":"Generation of C7H7+ cations with isomerization reactions","volume":"1091","author":"Kharnaior","year":"2016","journal-title":"Comput. Theor. Chem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1021\/jasms.3c00104","article-title":"Electrospray Ionization Tandem Mass Spectrometric Study of Selected Phosphine-Based Ligands for Catalytically Active Organometallics","volume":"34","author":"Fleissner","year":"2023","journal-title":"J. Am. Soc. Mass. Spectrom."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e5062","DOI":"10.1002\/jms.5062","article-title":"Electrospray ionization tandem mass spectrometry of 4-aryl-3,4-dihydrocoumarins","volume":"59","author":"Dias","year":"2024","journal-title":"J. Mass. Spectrom."},{"key":"ref_53","first-page":"3385","article-title":"Detection of menaquinone-6 and a novel methyl-substituted menaquinone-6 in Campylobacter jejuni and Campylobacter fetus subsp. fetus","volume":"129","author":"Carlone","year":"1983","journal-title":"J. Gen. Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Szterk, A., Bus, K., Zmys\u0142owski, A., and Ofiara, K. (2018). Analysis of Menaquinone-7 Content and Impurities in Oil and Non-Oil Dietary Supplements. Molecules, 23.","DOI":"10.3390\/molecules23051056"}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/3\/570\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T05:13:02Z","timestamp":1770441182000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/3\/570"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,5]]},"references-count":54,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["foods15030570"],"URL":"https:\/\/doi.org\/10.3390\/foods15030570","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,5]]}}}