{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T06:01:07Z","timestamp":1794376867905,"version":"4.9.0"},"reference-count":63,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973465"],"award-info":[{"award-number":["81973465"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"2023 Basic Research Project of Colleges and Universities of Liaoning Provincial Department of Educatio","award":["JYTZD2023137"],"award-info":[{"award-number":["JYTZD2023137"]}]},{"name":"Liaoning Province Science and Technology Plan Joint Plan","award":["2024-MSLH-448"],"award-info":[{"award-number":["2024-MSLH-448"]}]},{"name":"Faculty Resource Research Initiation Programs for Newly Introduced PhDs of Shenyang Pharmaceutical University","award":["2411520022"],"award-info":[{"award-number":["2411520022"]}],"id":[{"id":"https:\/\/ror.org\/03dnytd23","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>The rhizome of Polygonatum sibiricum (PR) is a widely used medicinal and edible herb valued worldwide. Traditional processing by repeated steaming and sun-drying empirically enhances its therapeutic properties, primarily through alterations in its bioactive polysaccharides. This study aimed to elucidate the structure\u2013activity relationship by systematically investigating the structural features and renoprotective activities of polysaccharides from PR and processed PR (PPR). Polysaccharides from PR and PPR (designated PRP and PPRP, respectively) were isolated and identified as five fractions (PRP-1, PRP-2, PPRP-1, PPRP-2, and PPRP-3). PPRP exhibited a broader molecular weight distribution (1.9\u201321.5 kDa) compared to PRP (2.6 kDa), indicating degradation and repolymerization. 1D\/2D NMR analysis revealed that PRP-1 contained a backbone of \u21921)-\u03b2-D-Fruf-(2\u2192, while processing introduced an additional \u21925)-\u03b1-L-Araf-(1\u2192 linkage into PPRP-1. The acidic fractions, PPRP-2 and PPRP-3, showed a partial overlap of motifs, including \u21924)-\u03b1-D-GalAp-(1\u2192 (in both methylated and non-methylated forms), \u21924)-\u03b2-D-Galp-(1\u2192, \u21925)-\u03b1-L-Araf-(1\u2192, and \u21922,4)-\u03b1-L-Rhap-(1\u2192. Notably, PPRP-2 demonstrated superior renoprotective activity in high glucose-induced NRK-52E cells. These structural transformations provide a molecular basis for the enhanced bioactivity and functional-food potential of polysaccharides.<\/jats:p>","DOI":"10.3390\/foods15040619","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T14:05:15Z","timestamp":1770645915000},"page":"619","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Steam-Driven Structural Remodeling of Polygonatum sibiricum Polysaccharides: Correlating Macromolecular Changes with Enhanced Renal Cell Protection"],"prefix":"10.3390","volume":"15","author":[{"given":"Hongyuan","family":"Ji","sequence":"first","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzhen","family":"Han","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anqi","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihui","family":"Li","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingni","family":"Pan","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumeng","family":"Ren","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Ren","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqiu","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,9]]},"reference":[{"key":"ref_1","unstructured":"Chinese Pharmacopoeia Commission (2022). The Pharmacopoeia of the People\u2019s Republic of China Vol. 1, China Medical Science Press."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1002\/fft2.231","article-title":"Huangjing\u2014From Medicine to Healthy Food and Diet","volume":"4","author":"Shi","year":"2023","journal-title":"Food Front."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Luo, L., Qiu, Y.X., Gong, L.M., Wang, W., and Wen, R.D. (2022). A Review of Polygonatum Mill. Genus: Its Taxonomy, Chemical Constituents, and Pharmacological Effect Due to Processing Changes. Molecules, 27.","DOI":"10.3390\/molecules27154821"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Liu, S., Jia, Q.J., Peng, Y.Q., Feng, T.H., Hu, S.T., Dong, J., and Liang, Z.S. (2022). Advances in Mechanism Research on Polygonatum in Prevention and Treatment of Diabetes. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.758501"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Rando, M.M., Biscetti, F., and Flex, A. (2025). Editorial: Diagnosis, Prevention and Treatment in Diabetic Nephropathy, Volume III. Front. Endocrinol., 16.","DOI":"10.3389\/fendo.2025.1586022"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e70098","DOI":"10.1002\/dmrr.70098","article-title":"Tubular Injury in Diabetic Kidney Disease: Early Diagnosis and Intervention Strategies","volume":"41","author":"Lv","year":"2025","journal-title":"Diabetes Metab. Res. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Xue, H., Yuan, P., Ni, J., Li, C., Shao, D., Liu, J., Shen, Y., Wang, Z., Zhou, L., and Zhang, W. (2013). H2S Inhibits Hyperglycemia-Induced Intrarenal Renin-Angiotensin System Activation via Attenuation of Reactive Oxygen Species Generation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0074366"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Klen, J., and Dol\u017ean, V. (2023). SGLT2 Inhibitors in the Treatment of Diabetic Kidney Disease: More than Just Glucose Regulation. Pharmaceutics, 15.","DOI":"10.3390\/pharmaceutics15071995"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1002\/jemt.24121","article-title":"Comprehensive Determination of the Processing Level of Rhizome of Polygonatum sibiricum by Macroscopic, Micromorphological, and Microscopic Characterizations","volume":"85","author":"Cheng","year":"2022","journal-title":"Microsc. Res. Tech."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"141911","DOI":"10.1016\/j.ijbiomac.2025.141911","article-title":"Physicochemical Characterization and Protective Effects of Raw and Nine-Steamed Polygonatum cyrtonema Polysaccharides on Cyclophosphamide-Induced Immunosuppression in Mice","volume":"307","author":"Luo","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1007\/s10719-021-10013-z","article-title":"Effect of Steaming Process on the Structural Characteristics and Antioxidant Activities of Polysaccharides from Polygonatum sibiricum rhizomes","volume":"38","author":"Li","year":"2021","journal-title":"Glycoconj. J."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"143168","DOI":"10.1016\/j.ijbiomac.2025.143168","article-title":"Effect of Structural Changes of Rehmannia glutinosa Polysaccharide before and after Processing on Anti-Aging Activity","volume":"309","author":"Shi","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jgr.2022.07.002","article-title":"Ginseng Polysaccharides: Potential Antitumor Agents","volume":"47","author":"Tao","year":"2023","journal-title":"J. Ginseng Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"119524","DOI":"10.1016\/j.carbpol.2022.119524","article-title":"Structural Characterization and Antioxidant Activity of Polygonatum sibiricum Polysaccharides","volume":"291","author":"Wang","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s40538-025-00768-0","article-title":"Structural Characterization and Bioactivity Evaluation of a Soluble Polysaccharide (PCHP-1A) from Polygonatum cyrtonema Hua: Antioxidant and \u03b1-glucosidase Inhibitory Activities","volume":"12","author":"Xia","year":"2025","journal-title":"Chem. Biol. Technol. Agric."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s13659-025-00542-7","article-title":"A Novel Polysaccharide in Polygonatum kingianum: Structure Elucidation, the Activities of Anti-Inflammatory and the Regulation of Gut Microbiota in Vitro","volume":"15","author":"Han","year":"2025","journal-title":"Nat. Prod. Bioprospect."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"124597","DOI":"10.1016\/j.carbpol.2025.124597","article-title":"The Change in Structure and Improvement of Anti-Aging Effects of Polysaccharides in Polygonati Rhizoma after the Traditional \u201cNine Steaming-Nine Sun-Drying\u201d","volume":"373","author":"Guan","year":"2026","journal-title":"Carbohydr. Polym."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Cheng, X.L., Ji, H.Y., Cheng, X., Wang, D.M., Li, T., Ren, K., Qu, S.H., Pan, Y.N., and Liu, X.Q. (2022). Characterization, Classification, and Authentication of Polygonatum sibiricum Samples by Volatile Profiles and Flavor Properties. Molecules, 27.","DOI":"10.3390\/molecules27010025"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Chen, Y., Liu, J., Xu, Y.F., Sun, C.Q., Qu, W.J., Du, H.C., He, M.L., Huo, J.S., Sun, J., and Huang, J. (2024). Comparison of Polygonatum Sibiricum Polysaccharides Found in Young and Mature Rhizomes. Foods, 13.","DOI":"10.3390\/foods13132010"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"141674","DOI":"10.1016\/j.foodchem.2024.141674","article-title":"Neutral Oligosaccharides from Ginseng (Panax ginseng) Residues vs. Neutral Ginseng Polysaccharides: A Comparative Study of Structure Elucidation and Biological Activity","volume":"464","author":"Tao","year":"2025","journal-title":"Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"127166","DOI":"10.1016\/j.ijbiomac.2023.127166","article-title":"Structural Characterization and Anti-Inflammatory Effects of Enteromorpha prolifera Polysaccharide-Fe\/Zn Complexes","volume":"253","author":"Feng","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Ren, Y., Bai, Y.P., Zhang, Z.D., Cai, W.L., and Del Rio Flores, A. (2019). The Preparation and Structure Analysis Methods of Natural Polysaccharides of Plants and Fungi: A Review of Recent Development. Molecules, 24.","DOI":"10.3390\/molecules24173122"},{"key":"ref_23","unstructured":"Chinese Pharmacopoeia Commission (2022). The Pharmacopoeia of the People\u2019s Republic of China Vol. 4, China Medical Science Press."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"107367","DOI":"10.1016\/j.fbio.2025.107367","article-title":"Evaluation of Probiotic Properties of Lactic Acid Bacteria and Physicochemical Characteristics of Fermented Jujube (Ziziphus jujuba Mill.) Polysaccharides","volume":"71","author":"Wang","year":"2025","journal-title":"Food Biosci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"114562","DOI":"10.1016\/j.bcp.2021.114562","article-title":"USP9X Deubiquitinates Connexin43 to Prevent High Glucose-Induced Epithelial-to-Mesenchymal Transition in NRK-52E Cells","volume":"188","author":"Sun","year":"2021","journal-title":"Biochem. Pharmacol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Liang, W., Chen, H., Huang, J., Zhao, L., and Yuan, Q. (2025). Structural Characterization and Immunomodulatory Activity of a Novel Mannoglucogalactan from Tremella aurantialba: Implications for Natural Immunotherapy. Foods, 14.","DOI":"10.3390\/foods14234126"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.molstruc.2005.05.015","article-title":"Spectroscopic Manifestation of Stretching Vibrations of Glycosidic Linkage in Polysaccharides","volume":"752","author":"Nikonenko","year":"2005","journal-title":"J. Mol. Struct."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"100168","DOI":"10.1016\/j.fochx.2021.100168","article-title":"Applications of Infrared Spectroscopy in Polysaccharide Structural Analysis: Progress, Challenge and Perspective","volume":"12","author":"Hong","year":"2021","journal-title":"Food Chem. X"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.carbpol.2012.09.068","article-title":"Structure and Protective Effect on UVB-Induced Keratinocyte Damage of Fructan from White Garlic","volume":"92","author":"Chen","year":"2013","journal-title":"Carbohydr. Polym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1007\/s00217-004-1098-8","article-title":"Heat-Induced Degradation of Inulin","volume":"220","author":"Kaiser","year":"2005","journal-title":"Eur. Food Res. Technol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1007\/s11947-021-02603-4","article-title":"Kinetics of Pectin Reactions in Apple Pomace during Hydrothermal Treatment","volume":"14","author":"Eblaghi","year":"2021","journal-title":"Food Bioprocess Technol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"102131","DOI":"10.1016\/j.fochx.2024.102131","article-title":"Glycan Degradation in Polygonati Rhizoma: Effects of Traditional \u2018Nine Steaming and Nine Basking\u2019 on Low Molecular Weight Fructans and Polysaccharides","volume":"25","author":"Mei","year":"2025","journal-title":"Food Chem. X"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"117962","DOI":"10.1016\/j.carbpol.2021.117962","article-title":"Triple-Helix Polysaccharides: Formation Mechanisms and Analytical Methods","volume":"262","author":"Guo","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_34","unstructured":"Biermann, C.J., and McGinnis, G.D. (1989). Analysis of Carbohydrates by GLC and MS, CRC Press."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.procbio.2019.06.017","article-title":"Physicochemical Characterization and Antioxidant Activity of Cell-Bound Exopolysaccharides from Lactobacillus fermentum S1 Obtained by Two Extraction Methods","volume":"85","author":"Wang","year":"2019","journal-title":"Process Biochem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"121829","DOI":"10.1016\/j.carbpol.2024.121829","article-title":"Structure Characterization of an agavin-type Fructan Isolated from Polygonatum cyrtonema and Its Effect on the Modulation of the Gut Microbiota in Vitro","volume":"330","author":"Gao","year":"2024","journal-title":"Carbohydr. Polym."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1021\/acs.chemrev.2c00580","article-title":"Primary Structure of Glycans by NMR Spectroscopy","volume":"123","author":"Fontana","year":"2023","journal-title":"Chem. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"130047","DOI":"10.1016\/j.ijbiomac.2024.130047","article-title":"Extraction and Characterization of Polysaccharides from Blackcurrant Fruits and Its Inhibitory Effects on Acetylcholinesterase","volume":"262","author":"Yang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e14380","DOI":"10.1111\/jfbc.14380","article-title":"Structural Characterization and Anti-inflammatory Activity of a Pectin Polysaccharide AP2 -c from the Lignified Okra","volume":"46","author":"Li","year":"2022","journal-title":"J. Food Biochem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"120023","DOI":"10.1016\/j.carbpol.2022.120023","article-title":"Structural Characteristic of Pectin-Glucuronoxylan Complex from Dolichos lablab L. Hull","volume":"298","author":"Liu","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Yang, Y.J., Yin, X.X., Zhang, D.J., Zhang, B.Y., Lu, J., and Wang, X.H. (2022). Structural Characteristics, Antioxidant, and Immunostimulatory Activities of an Acidic Polysaccharide from Raspberry Pulp. Molecules, 27.","DOI":"10.3390\/molecules27144385"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"111030","DOI":"10.1016\/j.lwt.2021.111030","article-title":"Effects of Temperature-Controlled Ultrasound Treatment on Sensory Properties, Physical Characteristics and Antioxidant Activity of Cloudy Apple Juice","volume":"142","author":"Shen","year":"2021","journal-title":"LWT"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"111833","DOI":"10.1016\/j.lwt.2021.111833","article-title":"Physicochemical Properties and Antioxidant Activity of Maillard Reaction Products Derived from Dioscorea opposita Polysaccharides","volume":"149","author":"Li","year":"2021","journal-title":"LWT"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Hu, S.J., Zhao, G.H., Zheng, Y., Qu, M., Jin, Q., Tong, C.Q., and Li, W. (2017). Effect of Drying Procedures on the Physicochemical Properties and Antioxidant Activities of Polysaccharides from Crassostrea gigas. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0188536"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"117220","DOI":"10.1016\/j.foodres.2025.117220","article-title":"Impact of Steam Explosion on Sorghum Starch Digestibility and Physicochemical Properties","volume":"221","author":"Ma","year":"2025","journal-title":"Food Res. Int."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"131746","DOI":"10.1016\/j.foodchem.2021.131746","article-title":"Steam Explosion Pretreatment of Achyranthis bidentatae Radix: Modified Polysaccharide and Its Antioxidant Activities","volume":"375","author":"Yi","year":"2022","journal-title":"Food Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"104866","DOI":"10.1016\/j.jff.2021.104866","article-title":"Effects of the Steaming Process on the Structural Properties and Immunological Activities of Polysaccharides from Polygonatum cyrtonema","volume":"88","author":"Wu","year":"2022","journal-title":"J. Funct. Foods"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"140778","DOI":"10.1016\/j.ijbiomac.2025.140778","article-title":"Study on Network Cross-Linked Hydrogel with Cationic Bletilla Striata Polysaccharide\/Carbopol as a Drug Delivery System","volume":"305","author":"Li","year":"2025","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"120219","DOI":"10.1016\/j.carbpol.2022.120219","article-title":"Structure, Stability, Antioxidant Activity, and Controlled-Release of Selenium Nanoparticles Decorated with Lichenan from Usnea longissima","volume":"299","author":"Yang","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"7651","DOI":"10.1080\/10408398.2025.2476118","article-title":"Polysaccharide-Based High Barrier Food Packaging Film: Design and Application","volume":"65","author":"Guo","year":"2025","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"125507","DOI":"10.1016\/j.ijbiomac.2023.125507","article-title":"Effects of Different Processing (Paozhi) on Structural Characterization and Antioxidant Activities of Polysaccharides from Cistanche deserticola","volume":"245","author":"Hou","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"6705","DOI":"10.2147\/IJN.S434196","article-title":"A Multi-Component Nano-Co-Delivery System Utilizing Astragalus Polysaccharides as Carriers for Improving Biopharmaceutical Properties of Astragalus Flavonoids","volume":"18","author":"Yang","year":"2023","journal-title":"Int. J. Nanomed."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"G\u00f3mez Vargas, C., Ponce, N.M.A., Stortz, C.A., Fissore, E.N., Bonelli, P., Ot\u00e1lora Gonz\u00e1lez, C.M., and Gerschenson, L.N. (2025). Pectin Obtention from agroindustrial wastes of Malus domestica Using Green Solvents (Citric Acid and Natural Deep Eutectic Solvents). Chemical, Thermal, and Rheological Characterization. Front. Chem., 12.","DOI":"10.3389\/fchem.2024.1504582"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1016\/j.biortech.2005.09.008","article-title":"Partial Characterization of Extracellular Polysaccharides from Cyanobacteria","volume":"97","author":"Parikh","year":"2006","journal-title":"Bioresour. Technol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"5813","DOI":"10.1021\/acs.jafc.6b01387","article-title":"Physicochemical Characterization of a Polysaccharide Fraction from Platycladus orientalis (L.) Franco and Its Macrophage Immunomodulatory and Anti-Hepatitis B Virus Activities","volume":"64","author":"Lin","year":"2016","journal-title":"J. Agric. Food Chem."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/j.1365-2613.2011.00764.x","article-title":"Fibroblasts and Myofibroblasts in Renal Fibrosis","volume":"92","author":"Meran","year":"2011","journal-title":"Int. J. Exp. Path."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.addr.2017.12.019","article-title":"Targeting Renal Fibrosis: Mechanisms and Drug Delivery Systems","volume":"129","author":"Nastase","year":"2018","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"5692852","DOI":"10.1155\/2016\/5692852","article-title":"Reviews on Mechanisms of In Vitro Antioxidant Activity of Polysaccharides","volume":"2016","author":"Wang","year":"2016","journal-title":"Oxid. Med. Cell. Longev."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.ijbiomac.2018.03.121","article-title":"Antioxidant and Antimicrobial Potential of Polysaccharides Sequentially Extracted from Polygonatum cyrtonema Hua","volume":"114","author":"Li","year":"2018","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"106485","DOI":"10.1016\/j.fbio.2025.106485","article-title":"Chemical Structures, Antioxidant Capacities, and Immunostimulatory Activities of Pectic Polysaccharides from Jujube Fruits Collected from Different Producing Areas","volume":"68","author":"Feng","year":"2025","journal-title":"Food Biosci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"114395","DOI":"10.1016\/j.foodres.2024.114395","article-title":"Potential Structure-Function Relationships of Pectic Polysaccharides from Quinoa Microgreens: Impact of Various Esterification Degrees","volume":"187","author":"Li","year":"2024","journal-title":"Food Res. Int."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1007\/978-981-13-8871-2_29","article-title":"Oxidative Stress and Renal Fibrosis: Mechanisms and Therapies","volume":"Volume 1165","author":"Liu","year":"2019","journal-title":"Renal Fibrosis: Mechanisms and Therapies"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"5136185","DOI":"10.1155\/2018\/5136185","article-title":"Structural Characterization, Antioxidant Activity, and Biomedical Application of Astragalus Polysaccharide Degradation Products","volume":"2018","author":"Wang","year":"2018","journal-title":"Int. J. Polym. 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