{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T15:16:50Z","timestamp":1771859810667,"version":"3.50.1"},"reference-count":59,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T00:00:00Z","timestamp":1771632000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32130099"],"award-info":[{"award-number":["32130099"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Chlorogenic acid from Eucommia ulmoides leaves is a promising natural antioxidant for food applications, yet its extraction and purification require optimization to improve yield and purity. This study employed ultrasound-assisted ethanol extraction on fresh leaves, evaluating the effects of ethanol concentration, solid-to-liquid ratio, extraction time, and temperature on CGA yield. Optimal extraction parameters were determined using response surface methodology. Purification with NKA-II macroporous resin increased CGA purity to 82.72%. SEM analysis revealed wrinkled and porous surface structures, while FTIR confirmed the presence of characteristic hydroxyl, carbonyl, and aromatic groups. Under optimized conditions (70% ethanol, solid-to-liquid ratio 1:20 g\/mL, 58 \u00b0C, 61 min), the extraction yield reached 6.96%. In vitro assays demonstrated strong antioxidant activity, with scavenging rates of 96.01% for DPPH, 89.69% for hydroxyl, and 99.82% for ABTS radicals at 5 mg\/mL. These findings provide an efficient method for obtaining chlorogenic acid from Eucommia ulmoides leaves and support its potential as a functional food ingredient.<\/jats:p>","DOI":"10.3390\/foods15040784","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T14:05:11Z","timestamp":1771855511000},"page":"784","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrasound-Assisted Extraction, Macroporous Resin Purification, and Antioxidant Activity of Chlorogenic Acid from Eucommia ulmoides Leaves"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5695-1456","authenticated-orcid":false,"given":"Qian","family":"Wang","sequence":"first","affiliation":[{"name":"College of Bioengineering, Henan University of Technology, Zhengzhou 450002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxiao","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Bioengineering, Henan University of Technology, Zhengzhou 450002, China"},{"name":"Henan Guang\u2019an Biotechnology Co., Ltd., Zhengzhou 450001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6750-5888","authenticated-orcid":false,"given":"Xia","family":"Xiong","sequence":"additional","affiliation":[{"name":"College of Bioengineering, Henan University of Technology, Zhengzhou 450002, China"},{"name":"Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yulong","family":"Yin","sequence":"additional","affiliation":[{"name":"Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keke","family":"Li","sequence":"additional","affiliation":[{"name":"Henan Golden Lily Biotechnology Co., Ltd., Anyang 455000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Bioengineering, Henan University of Technology, Zhengzhou 450002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s10522-025-10349-1","article-title":"Transcriptomic insights into the health span-enhancing effects of C. chinensis seed and E. ulmoides bark extracts in Caenorhabditis elegans","volume":"26","author":"Sayed","year":"2025","journal-title":"Biogerontology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e45453","DOI":"10.1097\/MD.0000000000045453","article-title":"Integrating UPLC\u2013Q-TOF\u2013MS and network pharmacology to analyze the components and mechanism of action of compound duzhong zhuangyao capsules on osteoporosis","volume":"104","author":"Qiu","year":"2025","journal-title":"Medicine"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Zhou, Y., Zhu, Y., Lv, H., Wang, Y., Shi, L., Wang, X., Chu, Q., and Jiang, T. 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