{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T15:50:24Z","timestamp":1783698624778,"version":"3.55.0"},"reference-count":43,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The National Key Research and Development Program of China","award":["2024YFD2100704"],"award-info":[{"award-number":["2024YFD2100704"]}]},{"name":"Key R&D Plan of Shandong Province","award":["2023CXGC010717"],"award-info":[{"award-number":["2023CXGC010717"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>To address the challenge of purifying bioactive polyphenols from the complex matrix of Ziziphus jujuba Mill. var. spinosa pulp, this study established an integrated purification protocol combining Deep Eutectic Solvent (DES) extraction with macroporous adsorption resin (MAR) enrichment. Among five screened resins, AB-8 exhibited superior selectivity, achieving a maximum adsorption capacity of 62.48 mg polyphenols\/g dry resin and a desorption ratio of 83.40%. Kinetic analysis revealed that the adsorption process strictly followed a pseudo-second-order model (R2 = 0.999), indicating a mechanism dominated by chemisorption. Through dynamic optimization, optimal column parameters were determined as a loading concentration of 2.4 mg\/mL, a flow rate of 1.0 mL\/min, and elution with 70% (v\/v) ethanol. Structural characterization via UV-Vis and FT-IR confirmed the effective removal of polysaccharide and protein impurities, while High-Performance Gel Permeation Chromatography (HPGPC) indicated a low-molecular-weight distribution (Mw approx. 1073 Da). Furthermore, HPLC-MS profiling definitively identified eight key constituents, including chlorogenic acid, catechin, rutin, and quercetin. Collectively, this work elucidates the adsorption mechanism and provides a scalable, efficient technical foundation for the high-purity preparation of jujube polyphenols.<\/jats:p>","DOI":"10.3390\/foods15040792","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T16:22:55Z","timestamp":1771863775000},"page":"792","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Targeted Separation of Ziziphus jujuba Pulp Polyphenols: Adsorption Kinetics Characteristics of AB-8 Resin and Product Structure Analysis"],"prefix":"10.3390","volume":"15","author":[{"given":"Dan","family":"Zhao","sequence":"first","affiliation":[{"name":"National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying 257000, China"},{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuzhi","family":"Xie","sequence":"additional","affiliation":[{"name":"National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying 257000, China"},{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beizhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujing","family":"Xuan","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nannan","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Medicine, Shandong Agricultural University, Taian 271018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Fan","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengzhong","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China"},{"name":"Institute of Food and Nutrition Development, Ministry of Agricultura and Rural Affairs, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying 257000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3351","DOI":"10.1021\/jf4007032","article-title":"The jujube (Ziziphus jujuba Mill.) fruit: A review of current knowledge of fruit composition and health benefits","volume":"61","author":"Gao","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"7871","DOI":"10.1021\/acs.jafc.2c01905","article-title":"Wild jujube (Ziziphus jujuba var. spinosa): A review of its phytonutrients, health benefits, metabolism, and applications","volume":"70","author":"Hua","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3019568","DOI":"10.1155\/2017\/3019568","article-title":"A review of dietary Ziziphus jujuba fruit (Jujube): Developing health food supplements for brain protection","volume":"2017","author":"Chen","year":"2017","journal-title":"Evid. -Based Complement. Altern. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.jclepro.2018.02.295","article-title":"Sustainable deep eutectic solvents preparation and their efficiency in extraction and enrichment of main bioactive flavonoids from sea buckthorn leaves","volume":"184","author":"Cui","year":"2018","journal-title":"J. Clean. Prod."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"24473","DOI":"10.1038\/s41598-025-07277-y","article-title":"Extraction and characterization of phenolic compounds of jujube (Ziziphus jujuba) by innovative techniques","volume":"15","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Frontini, A., Luvisi, A., Negro, C., Apollonio, M., Accogli, R., De Pascali, M., and De Bellis, L. (2024). Polyphenols extraction from different grape pomaces using natural deep eutectic solvents. Separations, 11.","DOI":"10.3390\/separations11080241"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1038\/s41598-023-46198-6","article-title":"Efficient extraction of oleoresin from Ferula gummosa roots by natural deep eutectic solvent and its structure and chemical characterizations","volume":"14","author":"Radmard","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"88","DOI":"10.15586\/qas.v17i4.1573","article-title":"Unlocking the production, bioactivity properties, and potential applications of hydrolyzed collagen from different sources: A review","volume":"17","author":"Shaik","year":"2025","journal-title":"Qual. Assur. Saf. Crops Foods"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"113729","DOI":"10.1016\/j.indcrop.2021.113729","article-title":"Adsorption\/desorption characteristics and chromatographic purification of polyphenols from Vernonia patula (Dryand.) Merr. using macroporous adsorption resin","volume":"170","author":"Hou","year":"2021","journal-title":"Ind. Crops Prod."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2400372","DOI":"10.1002\/jssc.202400372","article-title":"Macroporous polymeric resin for the purification of flavonoids from medicinal plants: A review","volume":"47","author":"Aljawarneh","year":"2024","journal-title":"J. Sep. Sci."},{"key":"ref_11","first-page":"58","article-title":"Enrichment of polyphenolic compounds from grape leaf extract (Vitis vinifera L.) using macroporous resin and investigating its adsorption kinetics and dynamics","volume":"7","author":"Sobhani","year":"2023","journal-title":"Trends Phytochem. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Tang, B., Wu, L., Wang, J., Sun, W., Zhao, Y., and Liu, F. (2021). Separation of heat-stable antifungal factor from Lysobacter enzymogenes fermentation broth via photodegradation and macroporous resin adsorption. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.663065"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5070","DOI":"10.1002\/fsn3.2464","article-title":"Purification and antioxidant properties of triterpenic acids from blackened jujube (Ziziphus jujuba Mill.) by macroporous resins","volume":"9","author":"Fu","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"130800","DOI":"10.1016\/j.foodchem.2021.130800","article-title":"Ultrasound-assisted adsorption\/desorption of jujube peel flavonoids using macroporous resins","volume":"368","author":"Shen","year":"2022","journal-title":"Food Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e122622","DOI":"10.1590\/fst.122622","article-title":"Process optimization of deep eutectic solvent-based microwave-assisted extraction of flavonoids from Ziziphi Spinosae Semen using response surface methodology","volume":"43","author":"Du","year":"2023","journal-title":"Food Sci. Technol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3403","DOI":"10.1021\/acs.jced.3c00706","article-title":"Choline chloride-and organic acids-based deep eutectic solvents: Exploring chemical and thermophysical properties","volume":"69","author":"Pinho","year":"2024","journal-title":"J. Chem. Eng. Data"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ling, J.K.U., and Hadinoto, K. (2022). Deep eutectic solvent as green solvent in extraction of biological macromolecules: A review. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23063381"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.foodchem.2018.06.100","article-title":"A comparative study on the adsorption and desorption characteristics of flavonoids from honey by six resins","volume":"268","author":"Lv","year":"2018","journal-title":"Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Che Zain, M.S., Lee, S.Y., Teo, C.Y., and Shaari, K. (2020). Adsorption and desorption properties of total flavonoids from oil palm (Elaeis guineensis Jacq.) mature leaf on macroporous adsorption resins. Molecules, 25.","DOI":"10.3390\/molecules25040778"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"7080","DOI":"10.1038\/s41598-025-91528-5","article-title":"Extraction and purification of total flavonoids from Zanthoxylum planispinum Var. Dintanensis leaves and effect of altitude on total flavonoids content","volume":"15","author":"Wang","year":"2025","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e17380","DOI":"10.1016\/j.heliyon.2023.e17380","article-title":"Effect of storage condition on the nutritional and anti-nutritional composition of kurkura (Ziziphus mauritiana Lam.) fruit from North-Eastern Ethiopia","volume":"9","author":"Tsegaye","year":"2023","journal-title":"Heliyon"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Decot, H., Sudhakaran, M., Boismier, E., Schilmiller, A., Claucherty, E., Doseff, A.I., and Aliakbarian, B. (2023). Tart Cherry (Prunus cerasus L.) Pit Extracts Protect Human Skin Cells against Oxidative Stress: Unlocking Sustainable Uses for Food Industry Byproducts. Foods, 12.","DOI":"10.3390\/foods12203748"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Resende, L.M., Oliveira, L.S., and Franca, A.S. (2023). Polyphenols in jabuticaba (Plinia spp.) peel flours: Extraction and comparative evaluation of FTIR and HPLC for quantification of individual compounds. Foods, 12.","DOI":"10.3390\/foods12071488"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"130743","DOI":"10.1016\/j.foodchem.2021.130743","article-title":"A new HPLC-MS\/MS method for the simultaneous determination of 36 polyphenols in blueberry, strawberry and their commercial products and determination of antioxidant activity","volume":"367","author":"Mustafa","year":"2022","journal-title":"Food Chem."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Wang, X., Su, J., Chu, X., Zhang, X., Kan, Q., Liu, R., and Fu, X. (2021). Adsorption and desorption characteristics of total flavonoids from Acanthopanax senticosus on macroporous adsorption resins. Molecules, 26.","DOI":"10.3390\/molecules26144162"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.colsurfb.2015.03.050","article-title":"Extraction optimization and nanoencapsulation of jujube pulp and seed for enhancing antioxidant activity","volume":"130","author":"Han","year":"2015","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"168639","DOI":"10.1016\/j.cej.2025.168639","article-title":"Multi-mechanism ultrasound enhanced macroporous resin flavonoid adsorption\/desorption: A green and sustainable process for balancing mass transfer efficiency and reusability","volume":"523","author":"Qian","year":"2025","journal-title":"Chem. Eng. J."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"144125","DOI":"10.1016\/j.foodchem.2025.144125","article-title":"Deep eutectic solvent extraction of polyphenol from plant materials: Current status and future prospects in food applications","volume":"482","author":"Sahu","year":"2025","journal-title":"Food Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"464621","DOI":"10.1016\/j.chroma.2023.464621","article-title":"Adsorption and desorption characteristics of flavonoids from white tea using macroporous adsorption resin","volume":"1715","author":"Yang","year":"2024","journal-title":"J. Chromatogr. A"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"116405","DOI":"10.1016\/j.lwt.2024.116405","article-title":"Purification of phenolics from Plantago depressa by macroporous resin: Adsorption\/desorption characteristics, chromatographic process development and UPLC-TQ-MS\/MS quantitative analysis","volume":"203","author":"Cao","year":"2024","journal-title":"LWT"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1007\/s11696-023-02665-y","article-title":"Ultrasonic-assisted extraction and enrichment of the flavonoids from Salicornia Europaea leaves using macroporous resins and response surface methodology","volume":"77","author":"Wang","year":"2023","journal-title":"Chem. Pap."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Agatonovic-Kustrin, S., Gegechkori, V., Petrovich, D.S., Ilinichna, K.T., and Morton, D.W. (2021). HPTLC and FTIR fingerprinting of olive leaves extracts and ATR-FTIR characterisation of major flavonoids and polyphenolics. Molecules, 26.","DOI":"10.3390\/molecules26226892"},{"key":"ref_33","first-page":"100608","article-title":"Extraction of polyphenolic compounds from rose and marigold, UPLC-ESI-QToF-MS\/MS, FTIR characterization and assessment of antioxidant activity","volume":"44","author":"Lekshmi","year":"2025","journal-title":"J. Appl. Res. Med. Aromat. Plants"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1016\/j.foodchem.2009.02.059","article-title":"Polyphenols from longan seeds and their radical-scavenging activity","volume":"116","author":"Zheng","year":"2009","journal-title":"Food Chem."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Li, Z., Wu, M., Wei, W., An, Y., Li, Y., Wen, Q., Zhang, D., Zhang, J., Yao, C., and Bi, Q. (2023). Fingerprinting evaluation and gut microbiota regulation of polysaccharides from Jujube (Ziziphus jujuba Mill.) Fruit. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24087239"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ji, X., Wang, Z., Hao, X., Zhu, Y., Lin, Y., Li, G., and Guo, X. (2022). Structural characterization of a new high molecular weight polysaccharide from jujube fruit. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.1012348"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Jia, F., Wang, B., Ma, H., Bai, C., and Zhang, Y. (2025). Research progress on extraction, separation, structure, and biological activities of polysaccharides from jujube fruit (Ziziphus jujuba Mill.): A review. Front. Chem., 13.","DOI":"10.3389\/fchem.2025.1581947"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"33219","DOI":"10.1039\/D1RA03640H","article-title":"New insight into the joint significance of dietary jujube polysaccharides and 6-gingerol in antioxidant and antitumor activities","volume":"11","author":"Wu","year":"2021","journal-title":"RSC Adv."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1486","DOI":"10.1111\/ijfs.13729","article-title":"Purification of polyphenols from kiwi fruit peel extracts using macroporous resins and high-performance liquid chromatography analysis","volume":"53","author":"Guo","year":"2018","journal-title":"Int. J. Food Sci. Technol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1080\/10942912.2022.2107009","article-title":"Purification and ultra-high-performance liquid chromatography tandem mass spectrometry analysis of phenolics extracted from male walnut flowers","volume":"25","author":"Yu","year":"2022","journal-title":"Int. J. Food Prop."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"359","DOI":"10.15586\/ijfs.v36i4.2674","article-title":"Enhancing stability and bioactivity of Chondrus ocellatus polyphenols through nanoparticle fabrication","volume":"36","author":"Shen","year":"2024","journal-title":"Ital. J. Food Sci.\/Riv. Ital. Sci. Aliment."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"58","DOI":"10.15586\/qas.v17i2.1528","article-title":"Microstructural characterization and evaluation of the physical and chemical quality of specialty roasted Coffea arabica L. with Pluma designation of origin","volume":"17","year":"2025","journal-title":"Qual. Assur. Saf. Crops Foods"},{"key":"ref_43","first-page":"193","article-title":"Phytochemical profile and biological activities of Brassica oleracea var. elongata leaf and seed extracts: An in vitro study","volume":"36","author":"Bouras","year":"2024","journal-title":"Ital. J. Food Sci.\/Riv. Ital. Di Sci. Degli Aliment."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/4\/792\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T16:29:38Z","timestamp":1771864178000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/4\/792"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,23]]},"references-count":43,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["foods15040792"],"URL":"https:\/\/doi.org\/10.3390\/foods15040792","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,23]]}}}