{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T07:29:30Z","timestamp":1787729370342,"version":"build-2784847793"},"reference-count":36,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:00:00Z","timestamp":1773014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"2024 Creative Research Project of the National Research Foundation of Korea","award":["R-2024-00456506"],"award-info":[{"award-number":["R-2024-00456506"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Fresh vegetables, especially green onions and chives, are raw ingredients widely consumed by Koreans, and have been linked to Listeria monocytogenes-induced food poisoning. This study aimed to assess microbial contamination levels in commercially available fresh-cut vegetables and produce, compare the effects of different types and concentrations of disinfectants on green onions and chives, and determine the growth rate of L. monocytogenes on chives under different storage conditions. Among the five fresh-cut vegetable mix salad products, the average total mesophilic count (TMC) was 2.00 log CFU\/g, whereas the crown daisies exhibited the highest levels of raw produce contamination (TMC of 4.14 log CFU\/g). The disinfection experiments indicated the elevated disinfectant capacities of electrolyzed water as well as washing under running water against Escherichia coli and L. monocytogenes. Enhanced anti-TMC ability of electrolyzed water were observed in acidic 30 ppm (pH 3.2) and 60 ppm (pH 5.6) of HOCl, and alkaline 100 ppm (pH 8.1) and 200 ppm (pH 8.8) of NaClO. Moreover, in the L. monocytogenes inoculation experiment in chive, the growth rates at 5 \u00b0C, 12 \u00b0C, and 30 \u00b0C were \u22120.002, 0.023, and 0.030 log CFU\/g\/h, respectively. This observation suggests that L. monocytogenes cannot grow on chives if stored at 5 \u00b0C but can at 12 \u00b0C. This study provides scientific evidence to guide the management of microbial quality of fresh produce and fresh-cut vegetables for safer meal provision in home and eating-out settings.<\/jats:p>","DOI":"10.3390\/foods15050957","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T17:16:03Z","timestamp":1773076563000},"page":"957","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of the Disinfection Capacity of Electrolyzed Water and Growth Rates of Listeria monocytogenes on Chives at Different Storages for Ensuring Microbiological Quality of Fresh Vegetable Foods"],"prefix":"10.3390","volume":"15","author":[{"given":"Hyeongmo","family":"An","sequence":"first","affiliation":[{"name":"Department of Food and Nutrition, Dankook University, Cheonan 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeja","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Food and Nutrition, Dankook University, Cheonan 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.13103\/JFHS.2024.39.4.335","article-title":"A study of microbial contamination in fresh-cut and ready-to-eat foods purchased from online markets","volume":"39","author":"Hwang","year":"2024","journal-title":"J. Food Hyg. Saf."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"669","DOI":"10.3201\/eid3104.240913","article-title":"Foodborne illness acquired in the United States-Major pathogens, 2019","volume":"31","author":"Walter","year":"2025","journal-title":"Emerg. Infect. Dis."},{"key":"ref_3","unstructured":"Centers for Disease Control and Prevention (CDC) (2025, July 30). Attribution of Foodborne Illness, Available online: https:\/\/www.cdc.gov\/food-safety\/php\/data-research\/foodborne-illness-sources\/index.html."},{"key":"ref_4","unstructured":"National Institute of Food and Drug Safety Evaluation (NIFDSE) (2024). Risk Assessment of Listeria Monocytogenes in Vegetables and Processed Vegetable Products, NIFDSE."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"495","DOI":"10.13103\/JFHS.2020.35.5.495","article-title":"Development of predictive models of Listeria monocytogenes in fresh-cut fruits and vegetables","volume":"35","author":"Kim","year":"2020","journal-title":"J. Food Hyg. Saf."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1108\/BFJ-06-2016-0283","article-title":"Does electrolyzed water have different sanitizing effects than sodium hypochlorite on different vegetable types?","volume":"119","author":"Ju","year":"2017","journal-title":"Br. Food J."},{"key":"ref_7","unstructured":"Bae, H.J. (2018). A study on improvement of usage standards for food sanitizers (chlorine dioxide, sodium hypochlorite). Final Research Report, Konkuk University Industry-University Cooperation Foundation."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.3201\/eid2610.191418","article-title":"Lessons learned from a decade of investigations of Shiga toxin\u2013producing Escherichia coli outbreaks linked to leafy greens, United States and Canada","volume":"26","author":"Marshall","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"274","DOI":"10.4315\/0362-028X-73.2.274","article-title":"Quantitative microbial risk assessment for Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes in leafy green vegetables consumed at salad bars","volume":"73","author":"Franz","year":"2010","journal-title":"J. Food Prot."},{"key":"ref_10","unstructured":"Ministry of Food and Drug Safety (MFDS) (2025, July 30). Safe Use of Food Sanitizers: Approved Seven Food-Grade Disinfectants including Hydrogen Peroxide, Ozone Water, Peracetic Acid, Sodium Hypochlorite, Calcium Hypochlorite, Hypochlorous Acid, and Chlorine Dioxide, Food Safety Knowledge Board, Available online: https:\/\/www.foodsafetykorea.go.kr\/portal\/board\/boardDetail.do?bbs_no=bbs001&menu_no=3120&ntctxt_no=1101916."},{"key":"ref_11","unstructured":"Ministry of Food and Drug Safety (MFDS) (2025, July 30). Field Guidelines for Food Sanitizers; Revised Edition, Available online: https:\/\/www.mfds.go.kr\/brd\/m_218\/view.do?seq=33466."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"108","DOI":"10.4315\/0362-028X.JFP-19-283","article-title":"Growth and survival of Listeria monocytogenes on intact fruit and vegetable surfaces during postharvest handling: A systematic literature review","volume":"83","author":"Marik","year":"2020","journal-title":"J. Food Prot."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"100259","DOI":"10.1016\/j.jfp.2024.100259","article-title":"Dynamics of Listeria monocytogenes and Salmonella enterica on cooked vegetables during storage","volume":"100","author":"Salazar","year":"2024","journal-title":"J. Food Prot."},{"key":"ref_14","unstructured":"Ministry of Food and Drug Safety (MFDS) (2025, July 30). Korean Food Code, Available online: https:\/\/www.foodsafetykorea.go.kr\/foodcode\/01_03.jsp."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1177\/104063870101300319","article-title":"Use of eosin methylene blue agar to differentiate Escherichia coli from other gram-negative mastitis pathogens","volume":"13","author":"Leininger","year":"2001","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_16","unstructured":"KisanBio Co., Ltd. (2025, July 30). MB Cell Product Information. Available online: https:\/\/www.kisanbio.com."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"110773","DOI":"10.1016\/j.foodres.2021.110773","article-title":"Synergistic interaction of tap water-based neutral electrolyzed water combined with UVA irradiation to enhance microbial inactivation on stainless steel","volume":"150","author":"Jee","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/0168-1605(94)90157-0","article-title":"A dynamic approach to predicting bacteria growth in food","volume":"23","author":"Baranyi","year":"1994","journal-title":"Int. J. Food Microbiol."},{"key":"ref_19","first-page":"287","article-title":"Analysis of salad purchaser types and purchasing behaviors through social network analysis","volume":"50","author":"Ha","year":"2022","journal-title":"J. Korean Soc. Qual. Manag."},{"key":"ref_20","unstructured":"Ministry of Education (2025, July 11). School Meal Hygiene Management Guidelines. Health Promotion Center, Available online: https:\/\/www.schoolkeepa.or.kr\/cop\/bbs\/selectArticleDetail.do?nttId=431&bbsId=BBSMSTR_000000000021."},{"key":"ref_21","unstructured":"Ministry of Food and Drug Safety (2025, July 11). Guidelines for the Children\u2019s Meal Management Support Center, Available online: https:\/\/dietary4u.mfds.go.kr\/board.es?mid=a61303000000&bid=0256&act=view&list_no=75288&tag=&nPage=1."},{"key":"ref_22","first-page":"68","article-title":"Microbiological safety assurance system for foodservice facilities","volume":"44","author":"Solberg","year":"1990","journal-title":"Agricultural and Food Sciences, Environmental Science Food Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.bjm.2017.01.010","article-title":"Prevalence and serotype distribution of Listeria monocytogenes isolated from foods in Montevideo-Uruguay","volume":"28","author":"Braga","year":"2017","journal-title":"Braz. J. Microbiol."},{"key":"ref_24","unstructured":"Korea Consumer Agency (2021). Safety survey on electrolyzed water generators. Safety Report, Korea Consumer Agency."},{"key":"ref_25","unstructured":"Tashiro, H., Kitahora, T., Sumiyoshi, K., Fujiyama, Y., and Bamba, T. (1995, January 3\u20137). Effect of functional water on gastrointestinal physiology. Proceedings of the Second Function Water Symposium \u201995, Kyoto, Japan."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3846\/jeelm.2020.11831","article-title":"Effect of ionised (electrolysed) water on the rat embryo development","volume":"28","author":"Cesiulis","year":"2020","journal-title":"J. Environ. Eng. Landsc. Manag."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"302","DOI":"10.4315\/0362-028X.JFP-16-246","article-title":"Quantitative microbial risk assessment for Escherichia coli O157:H7 in fresh-cut lettuce","volume":"80","author":"Pang","year":"2017","journal-title":"J. Food Proct."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Manyi-Loh, C.E., and Lues, R. (2025). Listeria monocytogenes and listeriosis: The global enigma. Foods, 14.","DOI":"10.3390\/foods14071266"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1093\/cid\/cis817","article-title":"Hospital-acquired listeriosis outbreak caused by contaminated diced celery in Texas, 2010","volume":"56","author":"Gaul","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"d3394","DOI":"10.1136\/bmj.d3394","article-title":"Outbreak of E. coli in Germany is linked to cucumbers from Spain","volume":"342","author":"Tuffs","year":"2011","journal-title":"Br. Med. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.4315\/0362-028X.JFP-14-468","article-title":"Growth of Salmonella enterica and Listeria monocytogenes on fresh-cut cantaloupe under different temperature abuse scenarios","volume":"78","author":"Huang","year":"2015","journal-title":"J. Food Prot."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1016\/j.foodcont.2018.12.035","article-title":"Growth and survival of Salmonella enterica and Listeria monocytogenes on fresh-cut produce and their juice extracts: Impacts and interactions of food matrices and temperature abuse conditions","volume":"100","author":"Huang","year":"2019","journal-title":"Food Control."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"87","DOI":"10.13103\/JFHS.2022.37.2.87","article-title":"Microbial qualities of parasites and foodborne pathogens in ready-to-eat (RTE) fresh-cut produces at the on\/offline markets","volume":"37","author":"Jeon","year":"2022","journal-title":"J. Food Hyg. Saf."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Feng, K., Ma, J., and Hu, W. (2024). Modelling the growth of Listeria monocytogenes on fresh-cut cucumbers at various storage temperatures. Horticulturae, 10.","DOI":"10.3390\/horticulturae10070667"},{"key":"ref_35","first-page":"813","article-title":"Retardation of Kimchi fermentation by the extracts of Allium tuberosum and growth inhibition of related microorganisms","volume":"27","author":"Kim","year":"1995","journal-title":"Korean J. Food Sci. Technol."},{"key":"ref_36","first-page":"196","article-title":"Growth retardation of Escherichia coli and Staphylococcus aureus by leek extract","volume":"30","author":"Lee","year":"2001","journal-title":"J. Korean Sci. Food Sci. Nutr."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/5\/957\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T17:20:49Z","timestamp":1773076849000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/5\/957"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,9]]},"references-count":36,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["foods15050957"],"URL":"https:\/\/doi.org\/10.3390\/foods15050957","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,9]]}}}