{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T16:49:26Z","timestamp":1774543766149,"version":"3.50.1"},"reference-count":55,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T00:00:00Z","timestamp":1774051200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Xinjiang Uygur Autonomous Region Tianshan Yingcai-Leading Talents in Scientific and Technological Innovation","award":["2022TSYCLJ0008"],"award-info":[{"award-number":["2022TSYCLJ0008"]}]},{"name":"Tianchi Yingcai Program","award":["2024000051"],"award-info":[{"award-number":["2024000051"]}]},{"name":"Xinjiang Uygur Autonomous Region Tianshan Cedar Plan\u2014Science and Technology Innovation Leaders Reserve Candidate Program","award":["2020XS08"],"award-info":[{"award-number":["2020XS08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Almond gum is a resource-rich natural polysaccharide; however, its high viscosity and low solubility severely limit industrial applications in separation, purification, and functional development. This study aimed to overcome these bottlenecks by optimizing an ethylenediaminetetraacetic acid (EDTA) preparation process and evaluating its protective efficacy against colitis. Using response surface methodology, optimal conditions were identified (1% EDTA, 3 h reaction, 10 h extraction), resulting in a modified polysaccharide (EAGP) with significantly reduced viscosity (from 640.8 to 238.7 mPa\u00b7s). SEM-EDX confirmed that EDTA efficiently removed cross-linking metal ions (K, Ca, Mg), creating a porous structure that facilitates purification. The purified fraction, EAGP-W1, was characterized as an arabinogalactan primarily composed of galactose (40.51%) and arabinose (38.38%). In vivo experiments demonstrated that EAGP-W1 significantly alleviated DSS-induced colitis, reducing colonic shortening and histopathological damage (p &lt; 0.05). Mechanistically, EAGP-W1 reshaped the gut microbiota by downregulating pro-inflammatory genera and upregulating probiotics (p &lt; 0.05). This shift promoted the production of short-chain fatty acids (SCFAs) (p &lt; 0.05), thereby repairing the intestinal barrier and suppressing inflammation. Overall, this study establishes an efficient EDTA-based strategy for almond gum processing and elucidates its anti-inflammatory mechanism through the \u201cmicrobiota\u2013metabolite\u2013barrier\u201d axis, providing a theoretical basis for its development as a high-value functional food for gut health.<\/jats:p>","DOI":"10.3390\/foods15061103","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T08:47:42Z","timestamp":1774255662000},"page":"1103","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preparation, Characterization, and Anti-Colitis Activity of Low-Viscosity EDTA-Soluble Polysaccharides from Almond Gum"],"prefix":"10.3390","volume":"15","author":[{"given":"Munisa","family":"Dilixiati","sequence":"first","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization and Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100039, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zumrat","family":"Abudureyim","sequence":"additional","affiliation":[{"name":"Morphological Center, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi 830011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nuermaimaiti","family":"Abudukelimu","sequence":"additional","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization and Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmidin","family":"Wali","sequence":"additional","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization and Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhua","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization and Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abulimiti","family":"Yili","sequence":"additional","affiliation":[{"name":"State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization and Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100039, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105013","DOI":"10.1016\/j.fbio.2024.105013","article-title":"Anti-melanogenic effect of a novel oligosaccharide derived from almond on forskolin-stimulated melanogenesis in B16F10 melanoma cells","volume":"62","author":"Kamilijiang","year":"2024","journal-title":"Food Biosci."},{"key":"ref_2","unstructured":"Ahmed, S., and Ali, A. 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