{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:07:40Z","timestamp":1792040860019,"version":"4.4.0"},"reference-count":25,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100015310","name":"Natural Science Foundation of Xinjiang Uygur Autonomous Region","doi-asserted-by":"publisher","award":["2024D01A74"],"award-info":[{"award-number":["2024D01A74"]}],"id":[{"id":"10.13039\/501100015310","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Basic Scientific Research Business Expenses Scientific research project of Autonomous Region University","award":["XJEDU2022P054"],"award-info":[{"award-number":["XJEDU2022P054"]}]},{"name":"Xinjiang Science and Technology Major Project","award":["2022A03018-1"],"award-info":[{"award-number":["2022A03018-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Acidic polysaccharides, valued for their outstanding bioactivity and physicochemical properties, represent a promising strategy for metabolic disease intervention. In this study, three acidic polysaccharide fractions (BRP-1, BRP-2, and BRP-3) were isolated from Brassica rapa L. using membrane filtration and ion-exchange chromatography. BRP-3, notable for its high galacturonic acid content (76.64%), was further purified to yield the homogeneous fraction BRP-3-1 (Mw = 22.3 kDa). Combining GC-MS, FTIR, and NMR analyses, we report for the first time the detailed structure of BRP-3-1\u2014a heteropolysaccharide composed of rhamnose (1.687%), galacturonic acid (75.584%), galactose (14.452%), and arabinose (8.277%)\u2014with a backbone composed with T-\u03b1-L-Araf-(1 \u2192 5)-\u03b1-L- Araf -(1 \u2192 4)-\u03b1-D-GalpA-(1 \u2192 4)-\u03b1-D-2-O- GalpA Me-(1 \u2192 4)-\u03b1-D-GalpA-(1 \u2192 4)-\u03b1-D-GalpA-(1 \u2192 3)-Galp-(1 \u2192 4)-\u03b1-D-GalpA, and T-Rhap, T-Galp as well as T-GalpA for branched chain and terminals. In HepG2 insulin-resistant cells, BRP-3-1 demonstrated potent dual regulation of glucose and lipid metabolism\u2014enhancing glucose consumption, lowering total cholesterol, and significantly reducing triglyceride levels in the high-dose group (800 \u03bcg\/mL), outperforming BRP-2. This work systematically defines the structure of a highly bioactive acidic polysaccharide from B. rapa L. and confirms its metabolic regulatory effects, offering a strong scientific foundation for its application in functional foods and as an adjuvant therapeutic for metabolic disorders.<\/jats:p>","DOI":"10.3390\/foods15071152","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T12:45:53Z","timestamp":1774615553000},"page":"1152","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Isolation, Purification, Structural Characterization of Acidic Polysaccharides from Brassica rapa L. Rhizomes and Their In Vitro Activity Verification in Ameliorating Glycolipid Metabolism Disorders"],"prefix":"10.3390","volume":"15","author":[{"given":"Sanawar","family":"Mansur","sequence":"first","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuhan","family":"Fang","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Li","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5626-2216","authenticated-orcid":false,"given":"Aytursun","family":"Abuduwaili","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering, Xinjiang Agricultural University, Urumqi 830052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmidin","family":"Wali","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anargvl","family":"Mahmut","sequence":"additional","affiliation":[{"name":"Xinjiang Institute of Materia Medica, Urumqi 830011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kailibinuer","family":"Abulaiti","sequence":"additional","affiliation":[{"name":"Xinjiang Institute of Materia Medica, Urumqi 830011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6056-0921","authenticated-orcid":false,"given":"Zulfiye","family":"Talat","sequence":"additional","affiliation":[{"name":"Xinjiang Institute of Materia Medica, Urumqi 830011, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6610-3092","authenticated-orcid":false,"given":"Weihao","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.ijbiomac.2010.07.008","article-title":"Composition analysis and anti-hypoxia activity of polysaccharide from Brassica rapa L.","volume":"47","author":"Xie","year":"2010","journal-title":"Int. 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