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This study evaluated the inactivation of T. gondii oocysts and bradyzoites (ME-49 strain) by Pulsed Electric Field technology (PEF). Treatment efficacy was determined by mouse bioassay combining brain qPCR and indirect immunofluorescence (IFA), with complementary qPCR in Hs27 cells. The infectious dose (ID50) of T. gondii was estimated at 34.6 oocysts. PEF-treated oocysts (15 kV\/cm; 50 kJ\/kg; 225 \u00b5s) showed a significant reduction in infectivity compared with untreated controls; accordingly, the dose required to establish infection increased to 85.3 oocysts after PEF treatment. Brain qPCR and IFA were highly correlated, whereas heart tissue was less sensitive. Bradyzoites recovered from PEF-treated meat (3.3 kV\/cm; 27 kJ\/kg; 1600 \u00b5s) showed a 50% infectivity reduction compared with untreated samples. In vitro assays confirmed an in vivo reduction in infectivity, indicating that cell cultures can serve as an ethical and efficient tool for preliminary viability assessment. This is the first evidence of T. gondii inactivation by PEF, highlighting its potential as a non-thermal strategy. Further studies are needed to optimize treatment parameters.<\/jats:p>","DOI":"10.3390\/foods15081447","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:42:45Z","timestamp":1776771765000},"page":"1447","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pulsed Electric Fields as an Effective Tool for Toxoplasma gondii Inactivation"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8889-1263","authenticated-orcid":false,"given":"Vanesa","family":"Abad","sequence":"first","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Instituto Agroalimentario de Arag\u00f3n, IA2 (Universidad de Zaragoza-CITA), 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5053-8309","authenticated-orcid":false,"given":"Daniel","family":"Berdejo","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Nutrici\u00f3n y Bromatolog\u00eda, Facultad de Veterinaria, Instituto Agroalimentario de Arag\u00f3n, IA2 (Universidad de Zaragoza-CITA), 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9337-417X","authenticated-orcid":false,"given":"Juan Manuel","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Instituto Agroalimentario de Arag\u00f3n, IA2 (Universidad de Zaragoza-CITA), 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabil","family":"Halaihel","sequence":"additional","affiliation":[{"name":"Departamento de Patolog\u00eda Animal, Sanidad Animal, Facultad de Veterinaria, Universidad de Zaragoza, 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1826-7582","authenticated-orcid":false,"given":"Jo\u00e3o Luis","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine, Protozoology Laboratory, Universidade Estadual de Londrina\u2014UEL, P.O. Box 6001, Londrina 86050-970, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2430-858X","authenticated-orcid":false,"given":"Ignacio","family":"\u00c1lvarez-Lanzarote","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Instituto Agroalimentario de Arag\u00f3n, IA2 (Universidad de Zaragoza-CITA), 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1668-4940","authenticated-orcid":false,"given":"Susana","family":"Bayarri","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Nutrici\u00f3n y Bromatolog\u00eda, Facultad de Veterinaria, Instituto Agroalimentario de Arag\u00f3n, IA2 (Universidad de Zaragoza-CITA), 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5049-3646","authenticated-orcid":false,"given":"Guillermo","family":"Cebri\u00e1n","sequence":"additional","affiliation":[{"name":"Departamento de Producci\u00f3n Animal y Ciencia de los Alimentos, Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria, Instituto Agroalimentario de Arag\u00f3n, IA2 (Universidad de Zaragoza-CITA), 50013 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1128\/MMBR.64.3.607-623.2000","article-title":"Lytic Cycle of Toxoplasma gondii","volume":"64","author":"Black","year":"2000","journal-title":"Microbiol. 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