{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:58:21Z","timestamp":1787169501540,"version":"build-2736575974"},"reference-count":49,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Terasi, or fermented shrimp paste, is a staple condiment in Indonesian cuisine, produced through spontaneous fermentation of small crustaceans under high-salt conditions. Despite its widespread culinary use, comprehensive studies examining both the microbiota and volatile compounds in commercial terasi remain scarce. This study aimed to characterize the microbial composition and volatile profiles of ten commercial terasi products sourced from different regions of Indonesia, representing both traditional home industries and large-scale manufacturers. Culture-dependent microbial enumeration and 16S rRNA gene sequencing were employed to assess microbial diversity, while volatile compounds were identified and quantified using gas chromatography\u2013mass spectrometry (GC-MS). Results revealed significant differences in microbial load and community composition among samples, with traditional products showing higher viable counts and microbial diversity, dominated by genera such as Tetragenococcus, Bacillus, Weissella, and Halanaerobium. Industrial samples, by contrast, contained no detectable microorganisms, likely due to sterilization practices for extended shelf life. A wide range of volatiles, including sulfur compounds, short-chain fatty acids, and trimethylamine, were identified across all samples, with a total of 48 detected compounds. Notably, correlation analysis revealed strong associations between specific bacterial genera and key volatile compounds, suggesting that microbial activity plays a central role in shaping terasi\u2019s flavor. This integrative analysis provides new insights into the microbial\u2013chemical interactions underlying fermented shrimp paste and offers potential applications for product standardization, starter culture development, and culinary innovation.<\/jats:p>","DOI":"10.3390\/foods15101623","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T10:39:47Z","timestamp":1778150387000},"page":"1623","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Profiling and Association of Microbiota and Volatile Compounds in Commercial Fermented Shrimp Pastes (Terasi)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8165-2072","authenticated-orcid":false,"given":"Reggie","family":"Surya","sequence":"first","affiliation":[{"name":"Food Technology Department, Faculty of Engineering, Bina Nusantara University, Jakarta 11480, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ervina","family":"Ervina","sequence":"additional","affiliation":[{"name":"Food Technology Department, Faculty of Engineering, Bina Nusantara University, Jakarta 11480, Indonesia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kantiya","family":"Petsong","sequence":"additional","affiliation":[{"name":"Department of Food Technology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Nugroho","sequence":"additional","affiliation":[{"name":"Department of Integrated Science, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,7]]},"reference":[{"key":"ref_1","first-page":"378","article-title":"The Indonesian fermented food product terasi: History and potential bioactivities","volume":"12","author":"Prihanto","year":"2021","journal-title":"Syst. Rev. Pharm."},{"key":"ref_2","unstructured":"Gardjito, M., Putri, R.G., and Dewi, S. (2017). Profil Struktur, Bumbu, dan Bahan dalam Kuliner Indonesia, Gadjah Mada University Press."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1186\/s42779-022-00142-7","article-title":"Diversity of sambals, traditional Indonesian chili pastes","volume":"9","author":"Surya","year":"2022","journal-title":"J. Ethn. Foods"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"012089","DOI":"10.1088\/1755-1315\/1169\/1\/012089","article-title":"Shrimp paste: Different processing and microbial composition across Southeast Asia","volume":"1169","author":"Pamungkaningtyas","year":"2023","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"100643","DOI":"10.1016\/j.ijgfs.2022.100643","article-title":"Effects of fermentation time on chemical, microbiological, antioxidant, and organoleptic properties of Indonesian traditional shrimp paste, terasi","volume":"31","author":"Surya","year":"2023","journal-title":"Int. J. Gastron. Food Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s42779-024-00222-w","article-title":"Terasi, exploring the Indonesian ethnic fermented shrimp paste","volume":"11","author":"Herlina","year":"2024","journal-title":"J. Ethn. Foods"},{"key":"ref_7","first-page":"985","article-title":"Strengthening the local economy through empowering shrimp production (product packaging design ideas)","volume":"4","author":"Arlina","year":"2024","journal-title":"Int. J. Educ. Soc. Stud. Manag."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/s42779-023-00218-y","article-title":"Characteristics of Indonesian traditional fermented seafood paste (terasi) made from shrimp and anchovy","volume":"11","author":"Surya","year":"2024","journal-title":"J. Ethn. Foods"},{"key":"ref_9","first-page":"117","article-title":"SWOT analysis for the development of small-scale shrimp paste enterprises","volume":"8","author":"Saediman","year":"2025","journal-title":"Int. J. Res. Eng. Sci. Manag."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"012049","DOI":"10.1088\/1755-1315\/116\/1\/012049","article-title":"Characterization of lactic acid bacteria (LAB) isolated from Indonesian shrimp paste (terasi)","volume":"116","author":"Amalia","year":"2018","journal-title":"IOP Conf. Ser. Earth Environ. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"279","DOI":"10.2323\/jgam.49.279","article-title":"Isolation and characterization of halophilic lactic acid bacteria isolated from \u201cterasi\u201d shrimp paste: A traditional fermented seafood product in Indonesia","volume":"49","author":"Kobayashi","year":"2003","journal-title":"J. Gen. Appl. Microbiol."},{"key":"ref_12","first-page":"1031","article-title":"A comparative study on chemical characteristics of Indonesian terasies, traditional salted seafoods","volume":"20","author":"Ambarita","year":"2021","journal-title":"Indian J. Tradit. Knowl."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"561","DOI":"10.17576\/jsm-2020-4903-11","article-title":"Identification of key sensory attributes of sambal-terasi, impact of different type of terasi, chemical characteristics and salt addition","volume":"49","author":"Ambarita","year":"2020","journal-title":"Sains Malays."},{"key":"ref_14","first-page":"1454","article-title":"Chemical compositions, sensory and antioxidative properties of salted shrimp paste (ka-pi) in Thailand","volume":"22","author":"Pongsetkul","year":"2015","journal-title":"Int. Food Res. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"101169","DOI":"10.1016\/j.fbio.2021.101169","article-title":"Bacterial composition changes and volatile compounds during the fermentation of shrimp paste: Dynamic changes of microbial communities and flavor composition","volume":"43","author":"Che","year":"2021","journal-title":"Food Biosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"101214","DOI":"10.1016\/j.ijgfs.2025.101214","article-title":"Characterization of microbiological and physisochemical changes in traditional Thai pla ra during two-stage fermentation of snakehead fish (Channa striata)","volume":"41","author":"Thinthasit","year":"2025","journal-title":"Int. J. Gastron. Food Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"100268","DOI":"10.1016\/j.focha.2023.100268","article-title":"Nutritional evaluation, flavor characteristics and microbial community of shrimp paste made from different materials and variance analysis","volume":"2","author":"Li","year":"2023","journal-title":"Food Chem. Adv."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"116534","DOI":"10.1016\/j.lwt.2024.116534","article-title":"Changes in bacterial flora and flavor of shrimp paste under different salt concentrations","volume":"205","author":"Huang","year":"2024","journal-title":"LWT"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Velasquez, M.T., Ramezani, A., Manal, A., and Raj, D.S. (2016). Trimethylamine N-oxide: The good, the bad and the unknown. Toxins, 8.","DOI":"10.3390\/toxins8110326"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1111\/1462-2920.14461","article-title":"Microbial trimethylamine metabolism in marine environments","volume":"21","author":"Sun","year":"2019","journal-title":"Environ. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1636S","DOI":"10.1093\/jn\/136.6.1636S","article-title":"The sulfur-containing amino acids: An overview","volume":"136","author":"Brosnan","year":"2006","journal-title":"J. Nutr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"104694","DOI":"10.1016\/j.tifs.2024.104694","article-title":"Recent advances on shrimp paste: Key flavor components and biochemical formation pathway, biogenic amine formation, microbial functions and innovative process strategies","volume":"152","author":"Hu","year":"2024","journal-title":"Trends Food Sci. Technol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s11157-017-9431-4","article-title":"Microbial production of volatile fatty acids: Current status and future perspectives","volume":"16","author":"Bhatia","year":"2017","journal-title":"Rev. Environ. Sci. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1700253","DOI":"10.1002\/ejlt.201700253","article-title":"Changes in lipids of shrimp (Acetes vulgaris) during salting and fermentation","volume":"119","author":"Pongsetkul","year":"2017","journal-title":"Eur. J. Lipid Sci. Technol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4512","DOI":"10.1080\/10406638.2022.2092873","article-title":"Advances in the synthesis and bio-applications if pyrazine derivatives: A review","volume":"43","author":"Choudhary","year":"2022","journal-title":"Polycycl. Aromat. Compd."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/j.tifs.2021.04.028","article-title":"Control strategies of pyrazines generation from Maillard reaction","volume":"112","author":"Yu","year":"2021","journal-title":"Trends Food Sci. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"K\u0142osowski, G., Mikulski, D., and Pielech-Przybylska, K. (2021). Pyrazines biosynthesis by Bacillus strains isolated from natto fermented soybean. Biomolecules, 11.","DOI":"10.3390\/biom11111736"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0168-1605(01)00688-2","article-title":"Fermentation and microflore of plaa-som, a Thai fermented fish product prepared with different salt concentrations","volume":"73","author":"Madsen","year":"2002","journal-title":"Int. J. Food Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.32732\/jfet.2024.13.1.1","article-title":"Preservation techniques to increase the shelf life of seafood products: An overview","volume":"13","author":"Rabiepour","year":"2024","journal-title":"J. Food Eng. Technol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"115277","DOI":"10.1016\/j.lwt.2023.115277","article-title":"Bacterial diversity and physicochemical properties of low-salt shrimp paste fermented at different temperatures","volume":"187","author":"Yang","year":"2023","journal-title":"LWT"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"128695","DOI":"10.1016\/j.foodchem.2020.128695","article-title":"Determination of microbial diversities and aroma characteristics of Beitang shrimp paste","volume":"344","author":"Yao","year":"2021","journal-title":"Food Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1228","DOI":"10.1080\/10408398.2019.1565491","article-title":"Quality, functionality, and microbiology of fermented fish: A review","volume":"60","author":"Zang","year":"2020","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1007\/s13213-012-0529-1","article-title":"Identification and histamine formation of Tetragenococcus isolated from Thai fermented food products","volume":"63","author":"Sitdhipol","year":"2013","journal-title":"Ann. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Sivamaruthi, B.S., Alagarsamy, K., Suganthy, N., Thangaleela, S., Kesika, P., and Chaiyasut, C. (2022). The role and significance of Bacillus and Lactobacillus species in Thai fermented foods. Fermentation, 8.","DOI":"10.3390\/fermentation8110635"},{"key":"ref_35","first-page":"101206","article-title":"Lactic acid bacteria in fermented fish: Enhancing flavor and ensuring safety","volume":"16","author":"Cai","year":"2024","journal-title":"J. Agric. Food Res."},{"key":"ref_36","first-page":"259","article-title":"Studies on the flavor compounds in traditional salt-fermented fishes","volume":"53","author":"Cha","year":"2020","journal-title":"Korean J. Fish. Aquat. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1007\/s11306-022-01902-x","article-title":"Metabolome analysis to investigate the effect of controlled fermentation on taste-related metabolites in terasi","volume":"18","author":"Sato","year":"2022","journal-title":"Metabolomics"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Helmi, H., Astuti, D.I., Putri, S.P., Sato, A., Lavina, W.A., Fukusaki, E., and Aditiawati, P. (2022). Dynamic changes in the bacterial community and metabolic profile during fermentation of low-salt shrimp paste (terasi). Metabolites, 12.","DOI":"10.3390\/metabo12020118"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Kurnianto, M.A., Adirama, S.I., Xu, W., Winarti, S., and Rini, D.M. (2025). Enhancing the quality of traditional Indonesian shrimp paste (terasi) through Tetragenococcus halophilus 54M106-3 inoculation: Physicochemical, sensory, and bioactivity insights. Foods, 14.","DOI":"10.3390\/foods14142419"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1080\/10498850.2018.1450573","article-title":"Effect of bacterial community and free amino acids on the content of biogenic amines during fermentation of yu-lu, a Chinese fermented fish sauce","volume":"27","author":"Wang","year":"2018","journal-title":"J. Aquat. Food Prod. Technol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Du, F., Zhang, X., Gu, H., Song, J., and Gao, X. (2019). Dynamic changes in the bacterial community during the fermentation of traditional Chinese fish sauce (TCFS) and their correlation with TCFS quality. Microorganisms, 7.","DOI":"10.3390\/microorganisms7090371"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3471","DOI":"10.1080\/87559129.2024.2358949","article-title":"Trimethylamine N-oxide (TMAO) in seafoods: Shared mechanisms between fish and humans for forming gut-microbial TMAO: Overview of animal TMAO-yield potential","volume":"40","author":"Niizeki","year":"2024","journal-title":"Food Rev. Int."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.lwt.2017.11.016","article-title":"Isolation of folate-producing Weissella spp. from Thai fermented fish (plaa som fug)","volume":"89","author":"Deatraksa","year":"2018","journal-title":"LWT"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"112763","DOI":"10.1016\/j.lwt.2021.112763","article-title":"Microbiological safety and microbiota of Kapi, Thai traditional fermented shrimp paste, from different sources","volume":"154","author":"Nakamura","year":"2022","journal-title":"LWT"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"102918","DOI":"10.1016\/j.fbio.2023.102918","article-title":"Comparative analysis of volatile compounds and microbial community succession in Chinese fish sauce: Three typical types of fast-fermented fish sauces","volume":"54","author":"Han","year":"2023","journal-title":"Food Biosci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"115275","DOI":"10.1016\/j.lwt.2023.115275","article-title":"Investigation of microbial ecology of salted seafood based on culture method and 16S rRNA metagenome sequencing","volume":"187","author":"Kim","year":"2023","journal-title":"LWT"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Chan, S.X.Y., Fitri, N., Mio Asni, N.S., Sayuti, N.H., Azlan, U.K., Qadi, W.S.M., Dawoud, E.A.D., Kamal, N., Sarian, M.N., and Mohd Lazaldin, M.A. (2023). A comprehensive review with future insights on the processing and safety of fermented fish and the associated changes. Foods, 12.","DOI":"10.3390\/foods12030558"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"6442","DOI":"10.1007\/s11947-025-03837-2","article-title":"Impact of bio-calcium from Nile tilapia bone as a supporting material on the stability enhancement of immobilized probiotics","volume":"18","author":"Petsong","year":"2025","journal-title":"Food Bioprocess Technol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s43014-025-00317-0","article-title":"Development of a novel protein and calcium fortified plant-based yogurt by addition of pea protein isolate and bio-calcium from Nile tilapia bones","volume":"7","author":"Karnjanapratum","year":"2025","journal-title":"Food Prod. Process. Nutr."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/10\/1623\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T11:40:52Z","timestamp":1778154052000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/10\/1623"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,7]]},"references-count":49,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["foods15101623"],"URL":"https:\/\/doi.org\/10.3390\/foods15101623","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,7]]}}}