{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T14:07:31Z","timestamp":1779286051178,"version":"3.51.4"},"reference-count":30,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Development and Application of Key Technologies for Microbial Germplasm Resource Mining","award":["(011) 2024-C1-F002*"],"award-info":[{"award-number":["(011) 2024-C1-F002*"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Infectious enteritis caused by bacterial pathogens are a significant global health concern, with high incidence and mortalities worldwide. The objective of this research was to explore the benefits of Lactiplantibacillus plantarum CCNH185 against Citrobacter rodentium-induced colitis in mice. Female C57BL\/6J mice (n = 8 per group) were orally administered L. plantarum CCNH185 at a dose of 2 \u00d7 109 CFU daily for 24 days, followed by a single oral challenge with C. rodentium (2 \u00d7 109 CFU) on day 21. L. plantarum CCNH185 significantly alleviated disease symptoms including body weight loss, colon shortening and histopathological damage (p &lt; 0.05). Treatment with L. plantarum CCNH185 also reduced pro-inflammatory cytokine levels, such as IL-1\u03b2 and IL-6 (p &lt; 0.05), while increasing anti-inflammatory IL-10 expression (p &lt; 0.05) in the colon. Histological and immunofluorescence demonstrated that L. plantarum CCNH185 improved the intestinal barrier integrity by increasing goblet cell numbers, upregulating MUC2 expression, reducing crypt hyperplasia, and suppressing epithelial cell apoptosis. Furthermore, transcriptomic analysis revealed that L. plantarum CCNH185 suppressed excessive immune cell infiltration and inflammatory responses in the colon during C. rodentium infection. Flow cytometry analysis further confirmed that L. plantarum CCNH185 suppressed hyperactivation of innate immune cells including macrophages, dendritic cells, neutrophils to alleviate inflammation. Furthermore, L. plantarum CCNH185 reshaped the gut microbiota by increasing the abundance of beneficial genera such as Lactobacillus, Dubosiella, and Romboutsia. Correlation analysis linked these microbial shifts with improved inflammatory and apoptotic markers. These findings highlight L. plantarum CCNH185 may serve as a promising preventive probiotic candidate for ameliorating infectious colitis possibly through strengthening the gut mucus barrier, modulating immune responses, and altering gut microbiota composition.<\/jats:p>","DOI":"10.3390\/foods15101815","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T12:30:51Z","timestamp":1779280251000},"page":"1815","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lactiplantibacillus plantarum CCNH185 Attenuates Citrobacter rodentium-Induced Colitis by Reshaping Gut Microbiota Structure and Modulating Innate Immunity"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3694-9327","authenticated-orcid":false,"given":"Yizhi","family":"Jing","sequence":"first","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyue","family":"Bai","sequence":"additional","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanzhi","family":"Yin","sequence":"additional","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinfeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junzhu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhichao","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8154-1540","authenticated-orcid":false,"given":"Zhengyuan","family":"Zhai","sequence":"additional","affiliation":[{"name":"College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanling","family":"Hao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health, China Agricultural University, Beijing 100193, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, B., Chen, D., Chen, H., Wu, W., Cheng, K., Tao, Y., Zhang, L., Liu, C., Ou, D., and Zhang, M. 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