{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T10:50:55Z","timestamp":1782211855136,"version":"3.54.5"},"reference-count":32,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T00:00:00Z","timestamp":1781913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Health Ministry","award":["I85E24000440005"],"award-info":[{"award-number":["I85E24000440005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>The European legislation sets the maximum residue levels for glyphosate in sesame seeds at 0.1 mg\/kg (EU Regulation n. 293\/2013) and for glufosinate and N-Acetyl-glufosinate expressed as glufosinate at 0.03 mg\/kg (EU Regulation n. 2016\/1002). The present work describes a rapid methodology to determine glyphosate, glufosinate and its metabolite and N-Acetyl-glufosinate in sesame seeds by LC\/MS\/MS. The method was studied in the framework of EU proficiency tests on sesame seeds. The analytical method was developed using methanol acidified with formic acid (1%, v\/v) extraction with an isotope internal standard, followed by LC\/MS\/MS detection. The recoveries were performed in the range of 0.05\u20130.5 mg\/kg for glyphosate and 0.02\u20130.2 mg\/kg for glufosinate and N-Acetyl-glufosinate. All the recovery values were between 70 and 114%, which is the acceptable interval according to SANTE\/11312\/2021; the relative standard deviation (%RSD) values met the requirement of &lt;20%. Linearity for each substance in solvent and matrix was studied, and the response was linear with R2 &gt; 0.999. We considered precision, matrix effect, LOD and LOQ in the validation. All the parameters were in agreement with the acceptability criteria of the document SANTE\/11312\/2021. The method was considered suitable for the determination of the studied substances on sesame seeds.<\/jats:p>","DOI":"10.3390\/foods15122233","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T08:19:52Z","timestamp":1782202792000},"page":"2233","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid Analysis of Glyphosate, Glufosinate and N-Acetyl Glufosinate in Sesame by Liquid Chromatography-Tandem Mass Spectrometry"],"prefix":"10.3390","volume":"15","author":[{"given":"Angela","family":"Santilio","sequence":"first","affiliation":[{"name":"Italian National Institute of Health, Department of Environment and Health, Viale Regina Elena 299, 00166 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana","family":"Girolimetti","sequence":"additional","affiliation":[{"name":"Italian National Institute of Health, Department of Environment and Health, Viale Regina Elena 299, 00166 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,20]]},"reference":[{"key":"ref_1","unstructured":"European Commission (2026, June 04). Commission Regulation (EC) No 293\/2013 of 20th March 2013 Amending Annex II and III to Regulation (EC) No 396\/2005 of the European Parliament and of the Council as Regards Maximum Residue Levels for Emamectin, Benzoate, Etofenprox, Etoxazole, Flutriafol, Glyphosate, Phosmet, Pyraclostrobin, Spinosad and Spirotetramat in or on Certain Products, Available online: https:\/\/eur-lex.europa.eu\/LexUriServ\/LexUriServ.do?uri=OJ:L:2013:096:0001:0030:en:PDF."},{"key":"ref_2","unstructured":"European Commission (2026, June 04). 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