{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:19:25Z","timestamp":1785417565840,"version":"3.56.0"},"reference-count":47,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Open Project Program of Xinghuacun College of Shanxi University","award":["XCSXU-KF-202322"],"award-info":[{"award-number":["XCSXU-KF-202322"]}]},{"award":["XCSXU-KF-202322"],"award-info":[{"award-number":["XCSXU-KF-202322"]}],"id":[{"id":"https:\/\/ror.org\/03y3e3s17","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20526"],"award-info":[{"award-number":["U23A20526"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["U23A20526"],"award-info":[{"award-number":["U23A20526"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Fundamental Research Program of Shanxi Province","award":["202403021221006"],"award-info":[{"award-number":["202403021221006"]}]},{"name":"Research and Innovation Team Project for Scientific Breakthroughs at Shanxi Bethune Hospital","award":["2024ZHANCHI06"],"award-info":[{"award-number":["2024ZHANCHI06"]}]},{"name":"The Key Research and Development (R&amp;D) Program of Shanxi Province","award":["2022ZDYF121"],"award-info":[{"award-number":["2022ZDYF121"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Vinasse, a major by-product of Fenjiu liquor production, is enriched with polysaccharides. However, their structural characteristics and potential anti-inflammatory functions remain unclear. In this study, polysaccharides from Fenjiu vinasse (VPS) were extracted and fractionated into seven sub-fractions (VPS-20 to VPS-80) via sequential alcohol precipitation. The molecular weight (Mw) of the fractions gradually decreased with increasing ethanol concentration. Monosaccharide composition analysis revealed that VPS and its fractions were mainly composed of glucose, mannose, and galactose. Among them, VPS-30 exhibited the most potent anti-inflammatory activity in LPS-induced RAW 264.7 macrophages by significantly reducing NO, IL-1\u03b2, IL-6, and TNF-\u03b1 production. Structural characterization by methylation analysis and NMR spectroscopy indicated that VPS-30 contained six major glycosidic linkages, including 2,3-Araf, T-Manp, 4-Manp, 3-Galp, 4-Glcp, and 4,6-Manp, and its detailed structure was predicted. Furthermore, in DSS-induced ulcerative colitis (UC) mice, VPS-30 markedly alleviated colonic inflammation, restored intestinal barrier integrity by upregulating MUC-2, occludin, and ZO-1, and reshaped gut microbiota diversity, particularly by increasing beneficial genera such as Limosilactobacillus, Butyricicoccus, and Ligilactobacillus. Overall, these findings suggest that Fenjiu vinasse polysaccharides exert significant anti-inflammatory effects and may serve as promising functional food candidates for UC intervention.<\/jats:p>","DOI":"10.3390\/foods15142575","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T13:25:46Z","timestamp":1784726746000},"page":"2575","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural Characteristics and Efficacy of Polysaccharides from Fenjiu Vinasse in Alleviating DSS-Induced Ulcerative Colitis"],"prefix":"10.3390","volume":"15","author":[{"given":"Huiqin","family":"Guo","sequence":"first","affiliation":[{"name":"Institute of Biotechnology, Biomedicine and Health Laboratory in Shanxi Province, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030002, China"},{"name":"Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan 030032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Biomedicine and Health Laboratory in Shanxi Province, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangying","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Biomedicine and Health Laboratory in Shanxi Province, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhua","family":"Shan","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Biomedicine and Health Laboratory in Shanxi Province, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoyu","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Biomedicine and Health Laboratory in Shanxi Province, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nifei","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology, Biomedicine and Health Laboratory in Shanxi Province, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiushan","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan 030032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"156893","DOI":"10.1016\/j.phymed.2025.156893","article-title":"The promoting effect of high-fat diet-induced trained immunity on ulcerative colitis and the mechanism of the therapeutic effect of Dahuang Mudan Decoction","volume":"143","author":"Li","year":"2025","journal-title":"Phytomedicine"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/ecco-jcc\/jjy215","article-title":"Neutrophil Extracellular Traps Sustain Inflammatory Signals in Ulcerative Colitis","volume":"13","author":"Dinallo","year":"2019","journal-title":"J. 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