{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T09:34:44Z","timestamp":1786440884198,"version":"build-2736575974"},"reference-count":72,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T00:00:00Z","timestamp":1786147200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005802","name":"MINISTRY OF RESEARCH, INNOVATION AND DIGITALIZATION","doi-asserted-by":"publisher","award":["7PFE\/2021"],"award-info":[{"award-number":["7PFE\/2021"]}],"id":[{"id":"10.13039\/501100005802","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006730","name":"MINISTRY OF EDUCATION AND RESEARCH","doi-asserted-by":"publisher","award":["PN 23 15 03 01"],"award-info":[{"award-number":["PN 23 15 03 01"]}],"id":[{"id":"10.13039\/501100006730","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>The industrial use of non-Saccharomyces yeasts offers a robust bioprocess strategy for modulating volatile biochemical profiles and reducing climate-driven ethanol production in fermented beverages. This study explores the metabolic diversity of indigenous non-Saccharomyces, with a particular focus on Starmerella bacillaris MI151, selected for sequential inoculation with Saccharomyces cerevisiae MI118. Pilot-scale bioreactor fermentations (25 L) of two Romanian matrix-specific aromatic grape cultivars, Busuioac\u0103 de Bohotin and T\u0103m\u00e2ioas\u0103 Rom\u00e2neasc\u0103, exhibited clear strain-dependent carbon flux changes. The sequential culture actively triggered the glycerol-pyruvic pathway, which consistently reduced the final ethanol concentration to up to 0.94% (v\/v) and maintained a balanced profile of volatile compounds. GC-MS-based metabolomic profiling revealed substantial changes in the esterification kinetics and glycosidic precursor cleavage. The sequential fermentation resulted in a synergistic increase in n-hexyl acetate (up to 2310.01 \u00b5g\/L) and bypassing of the standard enzymatic repression, freeing highly volatile monoterpenes (\u03b2-citronellol and \u03b2-geraniol) in the analyzed matrices. This targeted microbial system successfully modulated the organic acid\u2013phenolic balance, neutralizing harsh structural finishes, as confirmed by quantitative sensory mapping. Finally, these specific local yeast strains exhibit strong bioprocess scalability, offering a predictable, non-engineered strategy to lower the ethanol yield while driving targeted flavor enhancement in climate-vulnerable viticultural regions.<\/jats:p>","DOI":"10.3390\/foods15162789","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T01:20:03Z","timestamp":1786324803000},"page":"2789","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sequential Inoculation of Indigenous Starmerella bacillaris and Saccharomyces cerevisiae to Modulate the Volatile Profiles and Lower Ethanol Yields in Romanian Aromatic Wines"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9446-8409","authenticated-orcid":false,"given":"Raluca-\u0218tefania","family":"R\u0103doi-Encea","sequence":"first","affiliation":[{"name":"Faculty of Food Industry and Tourism, Transilvania University of Bra\u0219ov, 148 Castelului St., 500014 Bra\u0219ov, Romania"},{"name":"Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine Bucharest, 59 M\u0103r\u0103\u0219ti Blvd., District 1, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2275-9141","authenticated-orcid":false,"given":"Camelia-Filofteia","family":"Digu\u021b\u0103","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine Bucharest, 59 M\u0103r\u0103\u0219ti Blvd., District 1, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6434-1697","authenticated-orcid":false,"given":"Iuliana-Diana","family":"B\u0103rbulescu","sequence":"additional","affiliation":[{"name":"Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine Bucharest, 59 M\u0103r\u0103\u0219ti Blvd., District 1, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3442-3760","authenticated-orcid":false,"given":"Diana-Ionela","family":"Popescu (Steg\u0103ru\u0219)","sequence":"additional","affiliation":[{"name":"National Research and Development Institute for Cryogenic and Isotopic Technologies\u2014ICSI R\u00e2mnicu V\u00e2lcea, 4th Uzinei St., 240050 R\u00e2mnicu V\u00e2lcea, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2200-3988","authenticated-orcid":false,"given":"R\u0103zvan-Ionu\u021b","family":"Teodorescu","sequence":"additional","affiliation":[{"name":"Faculty of Land Reclamation and Environmental Engineering, University of Agronomic Sciences and Veterinary Medicine Bucharest, 011464 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandru-Dumitru","family":"Ilie","sequence":"additional","affiliation":[{"name":"Pietroasa-Istri\u021ba Research and Development Center for Viticulture and Pomiculture, 127470 Buz\u0103u, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7568-9952","authenticated-orcid":false,"given":"Florentina","family":"Matei","sequence":"additional","affiliation":[{"name":"Faculty of Food Industry and Tourism, Transilvania University of Bra\u0219ov, 148 Castelului St., 500014 Bra\u0219ov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wang, M., Wang, J., Chen, J., Philipp, C., Zhao, X., Wang, J., Liu, Y., and Suo, R. 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