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Cultivated meat could reduce the environmental impact of livestock production, but it remains costly, largely due to the expense of fetal bovine serum, a common cell culture media supplement. Algal and cyanobacterial proteins, particularly phycobiliproteins, represent promising sustainable sources of potentially bioactive peptides that may contribute to the development of serum-reduced or serum-free culture media. This study investigated the potential of hydrolysates prepared using the protein extracts of the red alga Porphyra haitanensis and the cyanobacterium Arthrospira platensis as functional supplements for cell cultivation in a low-serum environment. Twelve hydrolysates were generated by treating protein extracts from Arthrospira platensis and Porphyra haitanensis with six proteolytic enzymes: pepsin, trypsin, chymotrypsin, subtilisin, thermolysin and proteinase K. The effects of these hydrolysates were studied in three cell models of mammalian (CHO-K1), avian (QM7), and fish (MACK1) origin. The effects of hydrolysates on cell viability, proliferation, and antioxidant activity were evaluated. A. platensis\u2013chymotrypsin hydrolysate significantly improved QM7 cell viability compared to control in both 80% reduced-serum and tryptose phosphate broth conditions (p \u2264 0.0001). Additionally, peptide composition analysis and identification of amino acid sequences provided preliminary insights into potentially bioactive chromopeptides associated with the maintenance and promotion of cellular growth. Algae\/cyanobacteria-derived hydrolysates positively regulated cell viability and proliferation in serum-reduced conditions, although effects were dependent on the algal species, enzymatic treatment, and cell type. These findings highlight their potential as sustainable supplements for alternative cell culture media.<\/jats:p>","DOI":"10.3390\/foods15162941","type":"journal-article","created":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:37:34Z","timestamp":1787312254000},"page":"2941","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Algal and Cyanobacterial Hydrolysates as Serum-Reducing Supplements Across Mammalian, Avian and Fish Cells with Identification of Bioactive Chromopeptides"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7154-4214","authenticated-orcid":false,"given":"Nikolina","family":"Sibin\u010di\u0107-Georgieva","sequence":"first","affiliation":[{"name":"Innovative Centre, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8594-6736","authenticated-orcid":false,"given":"Ljubodrag","family":"Aleksi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Center of Excellence for Molecular Food Sciences, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2543-0838","authenticated-orcid":false,"given":"Na\u0111a","family":"Grozdani\u0107 Stanisavljevi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Oncology and Radiology of Serbia, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5810-1744","authenticated-orcid":false,"given":"Lora","family":"Tubi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Center of Excellence for Molecular Food Sciences, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo\u0161ko","family":"Tanaskovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Center of Excellence for Molecular Food Sciences, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8691-2486","authenticated-orcid":false,"given":"Nikola","family":"Gligorijevi\u0107","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Institute of Chemistry, Technology, and Metallurgy, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3282-064X","authenticated-orcid":false,"given":"Marija","family":"Stojadinovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Center of Excellence for Molecular Food Sciences, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simeon","family":"Mini\u0107","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Center of Excellence for Molecular Food Sciences, Faculty of Chemistry, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,21]]},"reference":[{"key":"ref_1","unstructured":"United Nations Population Fund (UNFPA) (2023). 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