{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:17:48Z","timestamp":1787807868198,"version":"build-2784847793"},"reference-count":44,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3606200"],"award-info":[{"award-number":["2023YFC3606200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science Foundation of Department of Education of Guangdong Province","award":["2023KTSCX055"],"award-info":[{"award-number":["2023KTSCX055"]}]},{"award":["2023KTSCX055"],"award-info":[{"award-number":["2023KTSCX055"]}],"id":[{"id":"https:\/\/ror.org\/01s5gyw91","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Conjugated linoleic acid (CLA) comprises a group of C18 fatty acids containing conjugated double bonds and has been associated with potential anti-obesity and antitumor effects. In this study, 116 presumptive lactic acid bacteria (LAB) isolates were recovered from homemade Sichuan pickles. Primary screening identified 28 CLA-producing isolates, among which strains 7# and 31# showed the highest absorbance at 233 nm (A233). CLA production by both strains was subsequently optimized and quantified using gas chromatography\u2013quadrupole time-of-flight mass spectrometry (GC-Q-TOF). Under the optimized conditions, strain 31# produced 66.50 \u00b1 3.80 \u03bcg\/mL total CLA, including 52.70 \u00b1 3.29 \u03bcg\/mL c9,t11-CLA and 13.80 \u00b1 0.51 \u03bcg\/mL t10,c12-CLA. Strain 7# produced 26.79 \u00b1 1.09 \u03bcg\/mL total CLA, including 14.05 \u00b1 0.49 \u03bcg\/mL c9,t11-CLA and 12.74 \u00b1 0.60 \u03bcg\/mL t10,c12-CLA. Physiological, biochemical, and safety assessments showed that strain 31# outperformed strain 7# overall, supporting its use in further product development and mechanistic studies. Functional annotation using the COG database and pathway mapping with KEGG identified candidate genes encoding an enzyme associated with linoleic acid isomerization in both strains. Potential mechanisms underlying their different CLA-producing capacities were also examined, providing a basis for the selection and development of high-CLA-producing strains.<\/jats:p>","DOI":"10.3390\/foods15172987","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:01:07Z","timestamp":1787670067000},"page":"2987","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Screening, Physiological Characterization, Genomic Analysis and Optimization by Conjugated Linoleic Acid Bioconversion of Two Lactiplantibacillus plantarum Strains"],"prefix":"10.3390","volume":"15","author":[{"given":"Mengting","family":"Hu","sequence":"first","affiliation":[{"name":"School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528453, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingbang","family":"Li","sequence":"additional","affiliation":[{"name":"School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528453, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528453, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528453, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528453, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4318-5132","authenticated-orcid":false,"given":"Lin","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Life Science and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China"},{"name":"Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanjin","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528453, China"},{"name":"Guangdong Engineering Technology Research Center of Medicine-Food Homologous Foods, Guangdong Pharmaceutical University, Zhongshan 528453, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6781-3418","authenticated-orcid":false,"given":"Xiaoyong","family":"Wu","sequence":"additional","affiliation":[{"name":"School of Food Science, Guangdong Pharmaceutical University, Zhongshan 528453, China"},{"name":"Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou 510006, China"},{"name":"Guangdong Engineering Technology Research Center of Medicine-Food Homologous Foods, Guangdong Pharmaceutical University, Zhongshan 528453, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Rastgoo, S., Shimi, G., Shiraseb, F., Karbasi, A., Ashtary-Larky, D., Yousefi, M., Golalipour, E., Asbaghi, O., and Zamani, M. 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