{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:30:34Z","timestamp":1789119034557,"version":"build-2803163510"},"reference-count":42,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009010","name":"Innovation and Entrepreneurship Development Capability Enhancement Engineering Technology Projects","doi-asserted-by":"publisher","award":["2021LYZKJXM011"],"award-info":[{"award-number":["2021LYZKJXM011"]}],"id":[{"id":"10.13039\/501100009010","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009010","name":"Henan Agricultural University Key R&D Center of Meat Science and Industrial Technology, Henan Province Key Research and Development Program","doi-asserted-by":"publisher","award":["241111110600"],"award-info":[{"award-number":["241111110600"]}],"id":[{"id":"10.13039\/501100009010","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Postgraduate Education Reform and Quality Improvement Project of Henan Province","award":["YJS2026SFGZZ08"],"award-info":[{"award-number":["YJS2026SFGZZ08"]}]},{"name":"Postgraduate Education Reform and Quality Improvement Project of Henan Province","award":["YJS2026YBGZZ23"],"award-info":[{"award-number":["YJS2026YBGZZ23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Huai chrysanthemum (Chrysanthemum morifolium Ramat. cv. Huaiju), collected from Jiaozuo, Henan Province, China, is a medicinal and edible plant rich in phenolic compounds. Polyphenols are natural bioactive compounds with potential antibacterial activity. In this study, HPLC-MS\/MS was used to identify and quantify the phenolic compounds in huai chrysanthemum polyphenol extract, and its antibacterial activity against representative Gram-negative and Gram-positive foodborne bacteria was evaluated. A total of 22 polyphenolic compounds were identified, including 10 phenolic acids, 10 flavonoids, and two other phenolic compounds. Caffeic acid was the predominant phenolic acid, while luteoloside was the predominant flavonoid. Flavonoids accounted for 94.03% of the total identified polyphenols. The extract inhibited the growth of both Escherichia coli and Staphylococcus aureus, with an MIC80 of 12.5 mg\/mL for both strains. Further analysis showed that the extract reduced intracellular protein content and ATP levels, increased extracellular alkaline phosphatase activity, altered cell surface charge, affected superoxide dismutase activity, and caused marked morphological changes in bacterial cells. These results suggested that the antibacterial effect of huai chrysanthemum polyphenol extract was related to cell envelope damage, energy metabolism disturbance, and redox imbalance. This study provided a basis for further application of huai chrysanthemum polyphenol extract as a natural antibacterial ingredient in food preservation.<\/jats:p>","DOI":"10.3390\/foods15183210","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T08:17:42Z","timestamp":1789114662000},"page":"3210","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antibacterial Activity and Cellular Effects of Huai Chrysanthemum Polyphenol Extract Against Foodborne Pathogens"],"prefix":"10.3390","volume":"15","author":[{"given":"Fuhua","family":"Zhang","sequence":"first","affiliation":[{"name":"Luohe Medical College, Luohe 462002, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Nan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan Agricultural University\/Henan Agricultural University Key R&D Center of Meat Science and Industrial Technology, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Shige","family":"Wen","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan Agricultural University\/Henan Agricultural University Key R&D Center of Meat Science and Industrial Technology, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Jiansheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Henan Shuanghui Investment Development Co., Ltd.\/Henan Technology Innovation Center of Meat Processing and Research, Luohe 462000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"}]},{"given":"Mingwu","family":"Qiao","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan Agricultural University\/Henan Agricultural University Key R&D Center of Meat Science and Industrial Technology, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Lianjun","family":"Song","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan Agricultural University\/Henan Agricultural University Key R&D Center of Meat Science and Industrial Technology, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"Xianqing","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan Agricultural University\/Henan Agricultural University Key R&D Center of Meat Science and Industrial Technology, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1820-5381","authenticated-orcid":false,"given":"Yan","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Food Science and Technology, Henan Agricultural University\/Henan Agricultural University Key R&D Center of Meat Science and Industrial Technology, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"ref_1","unstructured":"Chinese Pharmacopoeia Commission (2020). Pharmacopoeia of the People\u2019s Republic of China, China Medical Science Press. [2020 ed.]."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"118198","DOI":"10.1016\/j.jep.2024.118198","article-title":"Chrysanthemum morifolium as a traditional herb: A review of historical development, classification, phytochemistry, pharmacology and application","volume":"10","author":"Liu","year":"2024","journal-title":"J. Ethnopharmacol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1117","DOI":"10.1007\/s00438-016-1166-3","article-title":"Genetic diversity, population structure and association analysis in cut chrysanthemum (Chrysanthemum morifolium Ramat.)","volume":"291","author":"Li","year":"2016","journal-title":"Mol. Genet. Genom."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2786","DOI":"10.1007\/s13197-018-3203-1","article-title":"Chemical composition and antimicrobial activities of volatile oil extracted from Chrysanthemum morifolium Ramat","volume":"55","author":"Kuang","year":"2018","journal-title":"J. Food Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.jff.2019.04.053","article-title":"Chemical profile and in vitro gut microbiota modulatory, anti-inflammatory and free radical scavenging properties of Chrysanthemum morifolium Fubaiju","volume":"58","author":"Li","year":"2019","journal-title":"J. Funct. Foods"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"113043","DOI":"10.1016\/j.jep.2020.113043","article-title":"The flower head of Chrysanthemum morifolium Ramat. (Juhua): A paradigm of flowers serving as Chinese dietary herbal medicine","volume":"261","author":"Yuan","year":"2020","journal-title":"J. Ethnopharmacol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"499","DOI":"10.3136\/fstr.15.499","article-title":"Identification of Major Flavonoids in Petals of Edible chrysanthemum Flowers and Their Suppressive Effect on Carbon Tetrachloride-Induced Liver Injury in Mice","volume":"15","author":"Sugawara","year":"2020","journal-title":"Food Sci. Technol. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1002\/cbdv.202100206","article-title":"Comparison of Chemical Constituents and Pharmacological Effects of Different Varieties of chrysanthemum Flos in China","volume":"18","author":"Chen","year":"2021","journal-title":"Chem. Biodivers."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Yang, P., Yang, Y., He, C., Chen, Z., Yuan, Q., Zhao, S., Fu, Y., Zhang, P., and Mao, D. (2019). New caffeoylquinic acid derivatives and flavanone glycoside from the flowers of Chrysanthemum morifolium and their bioactivities. Molecules, 24.","DOI":"10.3390\/molecules24050850"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1002\/fsn3.71047","article-title":"Characterization of Polyphenolic Compounds and Antioxidant Activities of Tagetes Flowers with Varying Treatments Using LC-ESI-QToF-MS\/MS","volume":"13","author":"Siddiqa","year":"2025","journal-title":"Food Sci. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"113502","DOI":"10.1016\/j.jep.2020.113502","article-title":"Anti-inflammatory property and functional substances of Lonicerae Japonicae Caulis","volume":"267","author":"Su","year":"2021","journal-title":"J. Ethnopharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.jep.2012.09.003","article-title":"Hepatoprotective and antiviral properties of isochlorogenic acid A from Laggera alata against hepatitis virus infection","volume":"144","author":"Hao","year":"2012","journal-title":"J. Ethnopharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Lin, S., Ma, H., Zhang, S., Fan, W., Shen, C., Chen, J., Jin, M., Li, K., and He, Q. (2024). The combination of paeonol, diosmetin-7-O-\u03b2-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1332468"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1080\/10408398.2016.1196334","article-title":"Modifications of dietary flavonoids towards improved bioactivity: An update on structure-activity relationship","volume":"58","author":"Chen","year":"2018","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1080\/14786419.2023.2297403","article-title":"Phenolic compounds profile of optimised green and eco-friendly extracts of Eugenia pyriformis leaves, an alternative for antioxidant and antibacterial applications","volume":"39","author":"Dacoreggio","year":"2025","journal-title":"Nat. Prod. Res."},{"key":"ref_16","first-page":"21","article-title":"Extraction, preparative monomer separation and antibacterial activity of total polyphenols from Perilla frutescens","volume":"13","author":"Zhao","year":"2021","journal-title":"Food Funct."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"103618","DOI":"10.1016\/j.fbio.2024.103618","article-title":"Identification of polyphenol extracts from flaxseed and study on its bacteriostatic mechanism","volume":"58","author":"Wang","year":"2024","journal-title":"Food Biosci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/j.indcrop.2019.05.043","article-title":"Composition and antibacterial effect on food borne pathogens of Hibiscus surrattensis L. calyces essential oil","volume":"137","author":"Akarca","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3182","DOI":"10.1016\/j.foodres.2011.08.022","article-title":"Antioxidant and antimicrobial activity of legume hulls","volume":"44","author":"Kanatt","year":"2011","journal-title":"Food Res. Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3079","DOI":"10.1021\/acs.jafc.2c01262","article-title":"Synthesis, Structure Characterization and Antioxidant and Antibacterial Activity Study of Iso-orientin-Zinc Complex","volume":"70","author":"Wang","year":"2022","journal-title":"J. Agric. Food Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"101906","DOI":"10.1016\/j.fbio.2022.101906","article-title":"Inhibitory impacts of essential oil (Zanthoxylum schinifolium Sieb. et Zucc) on the growth of Staphylococcus epidermidis","volume":"49","author":"Wang","year":"2022","journal-title":"Food Biosci."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wang, L., Li, W., Li, X., Liu, J., and Chen, Y. (2023). Antimicrobial Activity and Mechanisms of Walnut Green Husk Extract. Molecules, 28.","DOI":"10.3390\/molecules28247981"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.jare.2023.04.017","article-title":"Antimicrobial activities of lavandulylated flavonoids in Sophora flavences against methicillin-resistant S. aureus via membrane disruption","volume":"57","author":"Weng","year":"2024","journal-title":"J. Adv. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.foodcont.2017.05.016","article-title":"Chemical composition, antibacterial activity of Cyperus rotundus rhizomes essential oil against Staphylococcus aureus via membrane disruption and apoptosis pathway","volume":"80","author":"Zhang","year":"2017","journal-title":"Food Control"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"106763","DOI":"10.1016\/j.fitote.2025.106763","article-title":"Plant polyphenols, Antibacterial activity and structural insights","volume":"185","author":"Li","year":"2025","journal-title":"Fitoterapia"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.mib.2013.09.007","article-title":"On the essentiality of lipopolysaccharide to Gram-negative bacteria","volume":"16","author":"Zhang","year":"2013","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_27","first-page":"20232","article-title":"Mammalian Cell Membrane Hybrid Polymersomes for mRNA Delivery","volume":"16","author":"Shin","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Xi, M., Hou, Y., Wang, R., Ji, M., Cai, Y., Ao, J., Shen, H., Li, M., Wang, J., and Luo, A. (2022). Potential Application of Luteolin as an Active Antibacterial Composition in the Development of Hand Sanitizer Products. Molecules, 27.","DOI":"10.3390\/molecules27217342"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1080\/13880209.2022.2117386","article-title":"Antioxidant properties of polyphenols from snow chrysanthemum (Coreopsis tinctoria) and the modulation on intestinal microflora in vitro","volume":"60","author":"Zhang","year":"2022","journal-title":"Pharm. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"5786","DOI":"10.1021\/acs.analchem.9b00014","article-title":"Mitochondria-Targeted Ratiometric Fluorescent Probe Based on Diketopyrrolopyrrole for Detecting and Imaging of Endogenous Superoxide Anion in Vitro and in Vivo","volume":"91","author":"Wang","year":"2019","journal-title":"Anal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.indcrop.2018.04.073","article-title":"The effect of water stress on phytochemical accumulation, bioactive compounds and expression of key genes involved in flavonoid biosynthesis in Chrysanthemum morifolium L","volume":"120","author":"Hodaei","year":"2018","journal-title":"Crops Prod."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"146788","DOI":"10.1016\/j.foodchem.2025.146788","article-title":"Unlocking the nutritional and phytochemical potential, metabolomics and bioaccessibility of polyphenols in south Indian edible flowers","volume":"496","author":"Prakruthi","year":"2025","journal-title":"Food Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.jff.2017.09.047","article-title":"The antibacterial activity and antibacterial mechanism of a polysaccharide from Cordyceps cicadae","volume":"38","author":"Zhang","year":"2017","journal-title":"J. Funct. Foods"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.lwt.2017.08.089","article-title":"Antibacterial mechanisms of Zataria multiflora Boiss. essential oil against Lactobacillus curvatus","volume":"87","author":"Ziaee","year":"2018","journal-title":"Lwt-Food Sci. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"118866","DOI":"10.1016\/j.carbpol.2021.118866","article-title":"Chitosan\/pullulan based films incorporated with clove essential oil loaded chitosan-ZnO hybrid nanoparticles for active food packaging","volume":"277","author":"Zhang","year":"2022","journal-title":"Carbohydr. Polym."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Barak, T., Karaca, B., Servi, H., Ertekin, S., Senturk, T., Dinc, M., Ustuner, H., and Eryilmaz, M. (2025). Antimicrobial, Antibiofilm, Anti-Quorum Sensing and Cytotoxic Activities of Dorystoechas hastata Boiss & Heldr. ex Bentham Essential Oil. Antibiotics, 14.","DOI":"10.3390\/antibiotics14101019"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"130340","DOI":"10.1016\/j.foodchem.2021.130340","article-title":"Evaluation of the membrane damage mechanism of protocatechualdehyde against Yersinia enterocolitica and simulation of growth inhibition in pork","volume":"363","author":"Tian","year":"2021","journal-title":"Food Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s43014-024-00244-6","article-title":"Screening of dandelion phenolic extracts and their anti-bacterial function against Escherichia coli through acting on Na+-K+ ATPase","volume":"6","author":"Xie","year":"2024","journal-title":"Food Prod. Process. Nutr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1016\/j.indcrop.2017.08.058","article-title":"Dodartia orientalis L. essential oil exerts antibacterial activity by mechanisms of disrupting cell structure and resisting biofilm","volume":"109","author":"Wang","year":"2017","journal-title":"Ind. Crops Prod."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"12918","DOI":"10.1111\/jfs.12918","article-title":"Antimicrobial activity and mechanism of limonene against Staphylococcus aureus","volume":"15","author":"Han","year":"2021","journal-title":"J. Food Saf."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1089\/mdr.2012.0244","article-title":"Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria","volume":"19","author":"Borges","year":"2013","journal-title":"Microb. Drug Resist."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1111\/j.1750-3841.2011.02213.x","article-title":"Antibacterial Activity and Mechanism of Action of Chlorogenic Acid","volume":"76","author":"Lou","year":"2011","journal-title":"J. Food Sci."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/18\/3210\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T09:04:50Z","timestamp":1789117490000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/18\/3210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":42,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["foods15183210"],"URL":"https:\/\/doi.org\/10.3390\/foods15183210","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]}}}