{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:08:56Z","timestamp":1790003336517,"version":"4.0.1"},"reference-count":55,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["956257"],"award-info":[{"award-number":["956257"]}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["10.55776\/I6939"],"award-info":[{"award-number":["10.55776\/I6939"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"award":["10.55776\/I6939"],"award-info":[{"award-number":["10.55776\/I6939"]}],"id":[{"id":"https:\/\/ror.org\/013tf3c58","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>\u03b2-Glucosidases (\u03b2GLs) play important roles in plant secondary metabolism by hydrolyzing glycosylated compounds involved in defense, signaling, and the formation of bioactive and flavor-related metabolites. In this study, a \u03b2-glucosidase from Fragaria \u00d7 ananassa (Fra-\u03b2GL; FxaC_10g48920.t1) was identified and biochemically characterized in comparison with a commercial \u03b2-glucosidase from Prunus dulcis (Pru-\u03b2GL). The Fra-\u03b2GL cDNA was cloned and heterologously expressed in Escherichia coli, and the recombinant protein was purified for biochemical characterization. Substrate screening demonstrated that Fra-\u03b2GL hydrolyzed the 7-O-glucosides of quercetin, kaempferol, and luteolin, whereas no detectable activity was observed toward the tested 3-O-glucosides, anthocyanin glucosides, arbutin, or phloridzin under the assay conditions. Kinetic analysis revealed higher catalytic efficiencies of Fra-\u03b2GL than Pru-\u03b2GL toward all four substrates examined. Fra-\u03b2GL showed its highest turnover rate and catalytic efficiency toward the synthetic substrate p-nitrophenyl-\u03b2-D-glucopyranoside (pNP-Glc), with a Kcat of 60.33 s\u22121 and a Kcat\/Km of 10.18 mM\u22121\u00b7s\u22121. Among the naturally occurring flavonoid glucosides, Fra-\u03b2GL exhibited the highest catalytic efficiency toward quercetin 7-O-glucoside (4.14 mM\u22121\u00b7s\u22121), approximately fivefold higher than that of Pru-\u03b2GL (0.824 mM\u22121\u00b7s\u22121). Thermal stability analysis showed progressive loss of activity with increasing temperature, with Pru-\u03b2GL retaining greater residual activity than Fra-\u03b2GL at elevated temperatures. Molecular docking suggested structural features that may contribute to the observed preference of Fra-\u03b2GL for flavonoid 7-O-glucosides over the corresponding 3-O-glucosides. Collectively, these findings demonstrate the substrate selectivity and distinct catalytic properties of Fra-\u03b2GL and identify Fra-\u03b2GL as a candidate for further investigation in the context of flavonoid glycoside metabolism in strawberry.<\/jats:p>","DOI":"10.3390\/foods15183290","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:12:09Z","timestamp":1789999929000},"page":"3290","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of a \u03b2-Glucosidase from Fragaria \u00d7 ananassa: Substrate Specificity, Catalytic Efficiency, and Structural Insights"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4498-8313","authenticated-orcid":false,"given":"Ibrahim","family":"Rabeeah","sequence":"first","affiliation":[{"name":"Research Group Phytochemistry and Biochemistry of Natural Compounds, Institute of Chemical, 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Industrial Systems and Technologies, University of Parma, Parco Area delle Scienze 181\/A, 43124 Parma, Italy"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Viktoria","family":"Gruber-Schmidt","sequence":"additional","affiliation":[{"name":"Research Group Phytochemistry and Biochemistry of Natural Compounds, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universit\u00e4t Wien, 1060 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Matthias","family":"Hackl","sequence":"additional","affiliation":[{"name":"Research Group Phytochemistry and Biochemistry of Natural Compounds, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universit\u00e4t Wien, 1060 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9642-8995","authenticated-orcid":false,"given":"Renate","family":"Paltram","sequence":"additional","affiliation":[{"name":"Research Group Phytochemistry and Biochemistry of Natural Compounds, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universit\u00e4t Wien, 1060 Wien, Austria"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"investigation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7808-5612","authenticated-orcid":false,"given":"Manfred","family":"G\u00f6ssinger","sequence":"additional","affiliation":[{"name":"Department of Fruit Processing, Federal College and Institute for Viticulture and Pomology, Wiener Stra\u00dfe 74, 3400 Klosterneuburg, 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Secondary Metabolites. Plant Physiology, Development and Metabolism, Springer Nature.","DOI":"10.1007\/978-981-99-5736-1"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1104\/pp.15.01499","article-title":"Focus Issue on Metabolism: Metabolites, Metabolites Everywhere","volume":"169","author":"Fernie","year":"2015","journal-title":"Plant Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1263\/jbb.102.8","article-title":"Phenylpropanoid metabolite supports cell aggregate formation in strawberry cell suspension culture","volume":"102","author":"Edahiro","year":"2006","journal-title":"J. Biosci. Bioeng."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5987","DOI":"10.1021\/jf402105g","article-title":"Alteration of the Content of Primary and Secondary Metabolites in Strawberry Fruit by Colletotrichum nymphaeae Infection","volume":"61","author":"Schmitzer","year":"2013","journal-title":"J. Agric. Food Chem."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Badmi, R., Gogoi, A., and Doyle Prestwich, B. (2023). Secondary Metabolites and Their Role in Strawberry Defense. Plants, 12.","DOI":"10.3390\/plants12183240"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"8496","DOI":"10.1021\/jf902103b","article-title":"Flavonoid Composition and Antioxidant Activity of Tree Peony (Paeonia Section Moutan) Yellow Flowers","volume":"57","author":"Li","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1016\/j.phytochem.2011.01.040","article-title":"Tannins in plant\u2013herbivore interactions","volume":"72","author":"Barbehenn","year":"2011","journal-title":"Phytochemistry"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1007\/s00425-017-2749-x","article-title":"Volatile terpenoids: Multiple functions, biosynthesis, modulation and manipulation by genetic engineering","volume":"246","author":"Abbas","year":"2017","journal-title":"Planta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1865","DOI":"10.1104\/pp.104.042580","article-title":"Functional Characterization of Enzymes Forming Volatile Esters from Strawberry and Banana","volume":"135","author":"Beekwilder","year":"2004","journal-title":"Plant Physiol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Sanchez-Ballesta, M.T., Maoz, I., and Figueroa, C.R. (2022). Editorial: Secondary metabolism and fruit quality. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.1072193"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Wang, F., Wang, J., Ji, G., Kang, X., Li, Y., Hu, J., Qian, C., and Wang, S. (2025). Regulation of Anthocyanins and Quality in Strawberries Based on Light Quality. Horticulturae, 11.","DOI":"10.3390\/horticulturae11040377"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1038\/s41438-021-00502-5","article-title":"Strawberry sweetness and consumer preference are enhanced by specific volatile compounds","volume":"8","author":"Fan","year":"2021","journal-title":"Hortic. Res."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Merecz-Sadowska, A., Sitarek, P., Kowalczyk, T., Zajdel, K., J\u0119cek, M., Nowak, P., and Zajdel, R. (2023). Food Anthocyanins: Malvidin and Its Glycosides as Promising Antioxidant and Anti-Inflammatory Agents with Potential Health Benefits. Nutrients, 15.","DOI":"10.3390\/nu15133016"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.3390\/ijms11041679","article-title":"Flavanols and Anthocyanins in Cardiovascular Health: A Review of Current Evidence","volume":"11","author":"Moreno","year":"2010","journal-title":"Int. J. Mol. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Le Roy, J., Huss, B., Creach, A., Hawkins, S., and Neutelings, G. (2016). Glycosylation Is a Major Regulator of Phenylpropanoid Availability and Biological Activity in Plants. Front. Plant Sci., 7.","DOI":"10.3389\/fpls.2016.00735"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"110904","DOI":"10.1016\/j.plantsci.2021.110904","article-title":"In through the out door: Biochemical mechanisms affecting flavonoid glycoside catabolism in plants","volume":"308","author":"Bozzo","year":"2021","journal-title":"Plant Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.1093\/pcp\/pcv151","article-title":"Functional Characterization and Substrate Promiscuity of UGT71 Glycosyltransferases from Strawberry (Fragaria \u00d7 ananassa)","volume":"56","author":"Song","year":"2015","journal-title":"Plant Cell Physiol."},{"key":"ref_18","first-page":"4","article-title":"Purification and biochemical characterization of a specific \u03b2-glucosidase from the digestive fluid of larvae of the palm weevil, Rhynchophorus palmarum","volume":"9","author":"Yapi","year":"2009","journal-title":"J. Insect Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Vass\u00e3o, D.G., Wielsch, N., Gomes, A.M.d.M.M., Gebauer-Jung, S., Hupfer, Y., Svato\u0161, A., and Gershenzon, J. (2018). Plant Defensive \u03b2-Glucosidases Resist Digestion and Sustain Activity in the Gut of a Lepidopteran Herbivore. Front. Plant Sci., 9.","DOI":"10.3389\/fpls.2018.01389"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Opassiri, R., Pomthong, B., Onkoksoong, T., Akiyama, T., Esen, A., and Ketudat Cairns, J.R. (2006). Analysis of rice glycosyl hydrolase family 1 and expression of Os4bglu12 \u03b2-glucosidase. BMC Plant Biol., 6.","DOI":"10.1186\/1471-2229-6-33"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wang, H., Zhang, Y., Feng, X., Peng, F., Mazoor, M.A., Zhang, Y., Zhao, Y., Han, W., Lu, J., and Cao, Y. (2022). Analysis of the \u03b2-Glucosidase Family Reveals Genes Involved in the Lignification of Stone Cells in Chinese White Pear (Pyrus bretschneideri Rehd.). Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.852001"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1007\/s00018-010-0399-2","article-title":"\u03b2-Glucosidases","volume":"67","author":"Esen","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"uhac089","DOI":"10.1093\/hr\/uhac089","article-title":"Roles of abscisic acid in regulating ripening and quality of strawberry, a model non-climacteric fruit","volume":"9","author":"Li","year":"2022","journal-title":"Hortic. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1007\/s10142-016-0510-3","article-title":"Extensive transcriptomic studies on the roles played by abscisic acid and auxins in the development and ripening of strawberry fruits","volume":"16","year":"2016","journal-title":"Funct. Integr. Genom."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1080\/14620316.2014.11513145","article-title":"Enzymatic and functional analysis of \u03b2-glucosidase FaBG1 during strawberry fruit ripening","volume":"89","author":"Zhang","year":"2014","journal-title":"J. Hortic. Sci. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1111\/tpj.12272","article-title":"The role of FaBG3 in fruit ripening and B. cinerea fungal infection of strawberry","volume":"76","author":"Li","year":"2013","journal-title":"Plant J."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Alger, E.I., Platts, A.E., Deb, S.K., Luo, X., Ou, S., Cao, Y., Hummer, K.E., Xiong, Z., Knapp, S.J., and Liu, Z. (2021). Chromosome-Scale Genome for a Red-Fruited, Perpetual Flowering and Runnerless Woodland Strawberry (Fragaria vesca). Front. Genet., 12.","DOI":"10.3389\/fgene.2021.671371"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon provides fast and bias-aware quantification of transcript expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"W228","DOI":"10.1093\/nar\/gkac278","article-title":"DeepLoc 2.0: Multi-label subcellular localization prediction using protein language models","volume":"50","author":"Thumuluri","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.3390\/foods14122064","article-title":"Gene Expression and Enzyme Kinetics of Polyphenol Oxidases in Strawberry and Their Possible Involvement in Enzymatic Browning Reactions in Strawberry Nectar","volume":"14","year":"2025","journal-title":"Foods"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Walker, J.M. (2009). The Lowry Method for Protein Quantitation. The Protein Protocols Handbook, Humana Press.","DOI":"10.1007\/978-1-59745-198-7"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1623","DOI":"10.1271\/bbb.59.1623","article-title":"Studies on \u03b2-Glucosidases from Soybeans That Hydrolyze Daidzin and Genistin: Isolation and Characterization of an Isozyme","volume":"59","author":"Matsuura","year":"1995","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_33","first-page":"100669","article-title":"Purification and characterization of an isoflavones conjugate hydrolyzing beta-glucosidase (ICHG) from Cyamopsis tetragonoloba (guar)","volume":"20","author":"Asati","year":"2019","journal-title":"Biochem. Biophys. Rep."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Rouyi, C., Baiya, S., Lee, S.K., Mahong, B., Jeon, J.S., Ketudat-Cairns, J.R., and Ketudat-Cairns, M. (2014). Recombinant expression and characterization of the cytoplasmic rice beta-glucosidase Os1BGlu4. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0096712"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1186\/s13068-019-1497-5","article-title":"Multifunctional elastin-like polypeptide renders \u03b2-glucosidase enzyme phase transition and high stability","volume":"12","author":"Zhou","year":"2019","journal-title":"Biotechnol. Biofuels"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"J. Comput. Chem."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1093\/bioinformatics\/btu031","article-title":"InterProScan 5: Genome-scale protein function classification","volume":"30","author":"Jones","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"8739","DOI":"10.1007\/s11033-020-05920-7","article-title":"Identification of GH1 gene family fgt members in Stevia rebaudiana and their expression when grown in darkness","volume":"47","author":"Yang","year":"2020","journal-title":"Mol. Biol. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.phytochem.2012.03.012","article-title":"Retargeting a maize \u03b2-glucosidase to the vacuole\u2014Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole","volume":"79","author":"Kiran","year":"2012","journal-title":"Phytochemistry"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1105\/tpc.112.095935","article-title":"A vacuolar beta-glucosidase homolog that possesses glucose-conjugated abscisic acid hydrolyzing activity plays an important role in osmotic stress responses in Arabidopsis","volume":"24","author":"Xu","year":"2012","journal-title":"Plant Cell"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1104\/pp.76.4.885","article-title":"Localization and Substrate Specificity of Glycosidases in Vacuoles of Nicotiana rustica","volume":"76","author":"Saunders","year":"1984","journal-title":"Plant Physiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"13737","DOI":"10.1038\/s41598-017-14239-6","article-title":"Gene expression atlas of fruit ripening and transcriptome assembly from RNA-seq data in octoploid strawberry (Fragaria\u2009\u00d7\u2009ananassa)","volume":"7","author":"Vallarino","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Chen, E.Y., Tan, C.M., Kou, Y., Duan, Q., Wang, Z., Meirelles, G.V., Clark, N.R., and Ma\u2019ayan, A. (2013). Enrichr: Interactive and collaborative HTML5 gene list enrichment analysis tool. BMC Bioinform., 14.","DOI":"10.1186\/1471-2105-14-128"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1038\/nrg2825","article-title":"Tackling the widespread and critical impact of batch effects in high-throughput data","volume":"11","author":"Leek","year":"2010","journal-title":"Nat. Rev. Genet."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1093\/bib\/bbs037","article-title":"Batch effect removal methods for microarray gene expression data integration: A survey","volume":"14","author":"Lazar","year":"2013","journal-title":"Brief. Bioinform."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1186\/s13059-018-1466-5","article-title":"Differential gene expression analysis tools exhibit substandard performance for long non-coding RNA-sequencing data","volume":"19","author":"Assefa","year":"2018","journal-title":"Genome Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/s41588-019-0356-4","article-title":"Origin and evolution of the octoploid strawberry genome","volume":"51","author":"Edger","year":"2019","journal-title":"Nat. Genet."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.1093\/molbev\/msab024","article-title":"Unraveling the Complex Hybrid Ancestry and Domestication History of Cultivated Strawberry","volume":"38","author":"Hardigan","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_50","unstructured":"Schrodinger, LLC. (2025, September 07). The PyMOL Molecular Graphics System, Version 1.8. Available online: https:\/\/www.scirp.org\/reference\/referencespapers?referenceid=3804901."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s13205-015-0328-z","article-title":"Catalytic properties, functional attributes and industrial applications of \u03b2-glucosidases","volume":"6","author":"Singh","year":"2015","journal-title":"3 Biotech"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1042\/bj3540037","article-title":"Crystal structure of a monocotyledon (maize ZMGlu1) \u03b2-glucosidase and a model of its complex with p-nitrophenyl \u03b2-d-thioglucoside","volume":"354","author":"Czjzek","year":"2001","journal-title":"Biochem. J."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"51","DOI":"10.5458\/jag.jag.JAG-2011_022","article-title":"Protein-carbohydrate Interactions Leading to Hydrolysis and Transglycosylation in Plant Glycoside Hydrolase Family 1 Enzymes","volume":"59","author":"Pengthaisong","year":"2012","journal-title":"J. Appl. Glycosci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"8907","DOI":"10.1021\/bi300675x","article-title":"Probing the Active Site Chemistry of \u03b2-Glucosidases along the Hydrolysis Reaction Pathway","volume":"51","author":"Badieyan","year":"2012","journal-title":"Biochemistry"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1080\/14756366.2019.1608198","article-title":"Probing the role of an invariant active site His in family GH1 \u03b2-glycosidases","volume":"34","author":"Strazzulli","year":"2019","journal-title":"J. Enzym. Inhib. Med. Chem."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/18\/3290\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:51:25Z","timestamp":1790002285000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/18\/3290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,17]]},"references-count":55,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["foods15183290"],"URL":"https:\/\/doi.org\/10.3390\/foods15183290","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,17]]}}}