{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:01:29Z","timestamp":1792789289312,"version":"4.5.0"},"reference-count":25,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&D Program of China","award":["2025YFC3509102"],"award-info":[{"award-number":["2025YFC3509102"]}]},{"name":"Key R&D Program Project (Competitive Platform) of Shandong Province","award":["2025CXPT123"],"award-info":[{"award-number":["2025CXPT123"]}]},{"name":"Science and Technology Development Fund of the Macao Special Administrative Region","award":["0005\/2024\/AKP"],"award-info":[{"award-number":["0005\/2024\/AKP"]}],"id":[{"id":"https:\/\/ror.org\/05vna4324","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Lonicerae japonicae Flos (LJF) is a medicinal and edible herbal material whose chemical characteristics and biological activity may vary with geographical origin. In this study, 27 LJF batches from Shandong, Henan, and Hebei Provinces were evaluated using headspace gas chromatography\u2013ion mobility spectrometry (HS\u2013GC\u2013IMS), multivariate analysis, and a mouse model of Staphylococcus aureus superinfection following influenza A (H1N1) virus infection. HS\u2013GC\u2013IMS detected 62 volatile ion signals; 49 were tentatively assigned to 36 unique volatile organic compounds (VOCs), while 13 remained unidentified. Fingerprint analysis revealed distinct origin-associated differences in volatile profiles, and OPLS\u2013DA showed the clearest separation of Shandong samples from those from Henan and Hebei (R2Y = 0.898; Q2 = 0.844). Thirty-two VIP-ranked signals were identified as candidate discriminant variables. In vivo, LJF aqueous extracts reduced the lung index, pulmonary bacterial burden, and relative NP RNA expression. Samples from Shandong and Henan generally showed greater improvements than those from Hebei across the evaluated endpoints. Overall, both the chemical and pharmacodynamic datasets indicated origin-associated variation in LJF. Direct relationships between specific volatile constituents and pharmacodynamic effects, however, were not established. These findings support the integration of HS\u2013GC\u2013IMS profiling with pharmacodynamic assessment as a practical framework for origin-related quality evaluation of LJF, while targeted confirmation of key discriminatory VOCs and further chemical\u2013efficacy analyses remain warranted.<\/jats:p>","DOI":"10.3390\/foods15183305","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:12:09Z","timestamp":1789999929000},"page":"3305","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Geographical Differentiation and Quality Evaluation of Lonicerae japonicae Flos by Integrating HS\u2013GC\u2013IMS Volatile Profiling with In Vivo Pharmacodynamic Assessment"],"prefix":"10.3390","volume":"15","author":[{"given":"Yanghai","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Ruijun","family":"Lyu","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Hongchao","family":"Shang","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Yuchen","family":"Yao","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Bonian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"},{"name":"Shandong Key Laboratory of Digital Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"},{"name":"Shandong Key Laboratory of Digital Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]},{"given":"Lu","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"},{"name":"Shandong Key Laboratory of Digital Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250355, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"158577","DOI":"10.1016\/j.phymed.2026.158577","article-title":"Lonicera trichosantha alleviates LPS-induced endometritis in mice by modulating the gut microbiota and host metabolism","volume":"159","author":"Chen","year":"2026","journal-title":"Phytomedicine"},{"key":"ref_2","first-page":"327","article-title":"Research Progress on Chemical Constituents, Pharmacological Effects and Modern Applications of Lonicerae Japonicae Flos","volume":"32","author":"Gao","year":"2026","journal-title":"Chin. J. Exp. Tradit. Med. Formulae"},{"key":"ref_3","first-page":"1061","article-title":"Fingerprint and in vitro antiviral activity of polysaccharides from Lonicerae japonicae Flos","volume":"31","author":"Ding","year":"2020","journal-title":"China Pharm."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Yang, R., Fang, L., Li, J., Zhao, Z., Zhang, H., and Zhang, Y. (2019). Separation of Five Iridoid Glycosides from Lonicerae japonicae Flos Using High-Speed Counter-Current Chromatography and Their Anti-Inflammatory and Antibacterial Activities. Molecules, 24.","DOI":"10.3390\/molecules24010197"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1824","DOI":"10.1002\/ptr.5688","article-title":"Flavonoids Isolated from Flowers of Lonicera japonica Thunb. Inhibit Inflammatory Responses in BV2 Microglial Cells by Suppressing TNF-\u03b1 and IL-\u03b2 Through PI3K\/Akt\/NF-kb Signaling Pathways","volume":"30","author":"Han","year":"2016","journal-title":"Phyther. Res."},{"key":"ref_6","first-page":"2085","article-title":"Comparison of odor and taste differences of Lonicerae Japonicae Flos from different producing areas based on HS-GC-MS and electronic sensory technology","volume":"55","author":"Ma","year":"2024","journal-title":"Chin. Tradit. Herb. Drugs"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1002\/pca.3319","article-title":"Quality evaluation of Lonicerae Japonicae Flos from different origins based on high-performance liquid chromatography (HPLC) fingerprinting and multicomponent quantitative analysis combined with chemical pattern recognition","volume":"35","author":"Zhang","year":"2024","journal-title":"Phytochem. Anal."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"117437","DOI":"10.1016\/j.jpba.2026.117437","article-title":"Identification of characteristic volatile compounds in Artemisiae argyi Levl. et Vant. from diverse origins using E-nose and HS-GC-IMS","volume":"274","author":"Ma","year":"2026","journal-title":"J. Pharm. Biomed. Anal."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"He, Y., Qin, H., Wen, J., Cao, W., Yan, Y., Sun, Y., Yuan, P., Sun, B., Fan, S., and Lu, W. (2023). Characterization of Key Compounds of Organic Acids and Aroma Volatiles in Fruits of Different Actinidia argute Resources Based on High-Performance Liquid Chromatography (HPLC) and Headspace Gas Chromatography-Ion Mobility Spectrometry (HS-GC-IMS). Foods, 12.","DOI":"10.3390\/foods12193615"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Li, C., Wan, H., Wu, X., Yin, J., Zhu, L., Chen, H., Song, X., Han, L., Yang, W., and Yu, H. (2022). Discrimination and Characterization of the Volatile Organic Compounds in Schizonepetae Spica from Six Regions of China Using HS-GC-IMS and HS-SPME-GC-MS. Molecules, 27.","DOI":"10.3390\/molecules27144393"},{"key":"ref_11","first-page":"3508","article-title":"Study on the Spectrum-Effect Correlation Quality Evaluation Model of Lonicerae Japonicae Flos Against Influenza Virus","volume":"28","author":"Gao","year":"2013","journal-title":"China J. Tradit. Chin. Med. Pharm."},{"key":"ref_12","first-page":"1495","article-title":"Experimental Study on Antiviral Effect of Lonicerae Japonicae Flos Aqueous Extract in Vitro","volume":"42","author":"Wang","year":"2015","journal-title":"Liaoning J. Tradit. Chin. Med."},{"key":"ref_13","first-page":"1031","article-title":"Study on Quality Evaluation System of Antibacterial Effect of Lonicerae Japonicae Flos Extract Based on Spectrum-Effect Correlation","volume":"44","author":"Gao","year":"2024","journal-title":"Chin. J. Pharm. Anal."},{"key":"ref_14","first-page":"1974","article-title":"Effects of Shugan Pingchuan Formula on Immune Balance in Asthmatic Mice Through TGF-\u03b21\/Smad Signaling Pathway","volume":"18","author":"Gu","year":"2023","journal-title":"World Chin. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"126158","DOI":"10.1016\/j.foodchem.2019.126158","article-title":"Recent progress in food flavor analysis using gas chromatography-ion mobility spectrometry (GC-IMS)","volume":"315","author":"Wang","year":"2020","journal-title":"Food Chem."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ren, P., Dao, R., Wang, L., Muhammad, N., Sang, Y., Liu, M., and Zhao, Z. (2025). HS-GC-IMS with sensory evaluation technique to analyze volatile flavor compounds of jujube flowers. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1590072"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"113074","DOI":"10.1016\/j.foodres.2023.113074","article-title":"Analysis of the effect of Tenebrio Molitor rennet on the flavor formation of Cheddar cheese during ripening based on gas chromatography-ion mobility spectrometry (GC-IMS)","volume":"171","author":"Song","year":"2023","journal-title":"Food Res. Int."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"5736","DOI":"10.1039\/D5AY00531K","article-title":"Deciphering the differences in aroma components of tobacco from different origins based on HS-GC-IMS and multivariate statistical analysis","volume":"17","author":"Li","year":"2025","journal-title":"Anal. Methods"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ouyang, X., Shi, X., Zhou, C., Li, M., Huang, R., Liu, H., Huang, D., and Zhang, G. (2025). An Exploratory Study on the Influence of Frying on Chemical Constituent Transformation and Antioxidant Activity in Ziziphi Spinosae Semen: A Multimodal Analytical Strategy Based on UPLC-Q-TOF-MS and GC-IMS. Foods, 14.","DOI":"10.3390\/foods14234145"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Mena-Garc\u00eda, A., Herrero-Guti\u00e9rrez, D., Sanz, M.L., D\u00edez-Municio, M., and Ruiz-Matute, A.I. (2023). Fingerprint of Characteristic Saffron Compounds as Novel Standardization of Commercial Crocus sativus Extracts. Foods, 12.","DOI":"10.3390\/foods12081634"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"106440","DOI":"10.1016\/j.microc.2021.106440","article-title":"Discrimination of authenticity of Fritillariae Cirrhosae Bulbus based on terahertz spectroscopy and chemometric analysis","volume":"168","author":"Du","year":"2021","journal-title":"Microchem. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"132932","DOI":"10.1016\/j.foodchem.2022.132932","article-title":"Screening of specific quantitative peptides of beef by LC-MS\/MS coupled with OPLS-DA","volume":"387","author":"Kang","year":"2022","journal-title":"Food Chem."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wu, H., Niu, L., Chen, J., Xu, H., Kong, C., and Xiao, J. (2025). A Comprehensive Metabolomic Analysis of Volatile and Non-Volatile Compounds in Folium Artemisia argyi Tea from Different Harvest Times. Foods, 14.","DOI":"10.3390\/foods14050843"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sun, Y., Yuan, P., Liu, G., Yang, Y., Shu, N., and Lu, W. (2025). Analysis of the Effects of Post-Fermentation Freezing Treatment on the Flavor Characteristics of Beibinghong Ice Wine by HPLC and HS-GC-IMS. Foods, 14.","DOI":"10.3390\/foods14091631"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"141074","DOI":"10.1016\/j.foodchem.2024.141074","article-title":"Effect of hot-air drying processing on the volatile organic compounds and maillard precursors of Dictyophora Rubrovalvata based on GC-IMS, HPLC and LC-MS","volume":"463","author":"Meng","year":"2025","journal-title":"Food Chem."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/18\/3305\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:33:48Z","timestamp":1790051628000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/18\/3305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":25,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["foods15183305"],"URL":"https:\/\/doi.org\/10.3390\/foods15183305","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]}}}