{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T16:00:13Z","timestamp":1790006413302,"version":"4.0.1"},"reference-count":32,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>Reheating alters residual molecular order and matrix architecture in retrograded starch, but these changes do not necessarily produce parallel changes in enzymic digestion. Gels of normal maize starch (NM), high-amylose maize starch (HM), and a 50\/50 (w\/w) NM\/HM blend were stored at 4 \u00b0C for 7 d and steam-reheated to core temperatures of 50 or 80 \u00b0C. HM was less hydrolysed than NM at either temperature. At 300 min, hydrolysis reached 51.3% and 61.4% for HM at 50 and 80 \u00b0C, respectively, versus approximately 81% for NM. DSC and XRD showed that HM-containing gels retained more thermally stable ordered structures. The 50\/50 blend responded differently: reheating to 80 \u00b0C lowered hydrolysis from 72.6% to 65.6% despite no corresponding increase in residual order. This inverse response coincided with lower pore area (12.8 to 4.6 m2\/g), larger 4 V\/A pore diameter (966 to 2623 nm), and a more continuous freeze-dried morphology, consistent with fewer fine mercury-accessible pores and internal interfaces. Overall, starch structural composition established the broad ranking of hydrolysis after reheating, whereas matrix reorganisation modified the relationship between residual order and enzymic susceptibility in the 50\/50 blend. Residual molecular order alone was therefore insufficient to predict the digestion response after reheating.<\/jats:p>","DOI":"10.3390\/foods15183344","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:12:09Z","timestamp":1789999929000},"page":"3344","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reheating-Induced Matrix Reorganisation Modulates the In Vitro Digestibility of a Retrograded Normal\/High-Amylose Maize Starch Blend"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0440-6393","authenticated-orcid":false,"given":"Zeping","family":"Shao","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China"},{"name":"Key Laboratory of Special Diet Nutrition and Health Research of China National Light Industry Council, School of Medicine, Nankai University, Tianjin 300350, China"},{"name":"Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3237-4109","authenticated-orcid":false,"given":"Caili","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China"},{"name":"Key Laboratory of Special Diet Nutrition and Health Research of China National Light Industry Council, School of Medicine, Nankai University, Tianjin 300350, China"},{"name":"Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, QLD 4072, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Gao","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China"},{"name":"Key Laboratory of Special Diet Nutrition and Health Research of China National Light Industry Council, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6173-1331","authenticated-orcid":false,"given":"Dan","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China"},{"name":"Key Laboratory of Special Diet Nutrition and Health Research of China National Light Industry Council, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Lv","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China"},{"name":"Key Laboratory of Special Diet Nutrition and Health Research of China National Light Industry Council, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin 300071, China"},{"name":"Key Laboratory of Special Diet Nutrition and Health Research of China National Light Industry Council, School of Medicine, Nankai University, Tianjin 300350, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.copbio.2019.10.008","article-title":"Resistant starch: Impact on the gut microbiome and health","volume":"61","author":"DeMartino","year":"2020","journal-title":"Curr. Opin. Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1111\/1541-4337.12143","article-title":"Starch retrogradation: A comprehensive review","volume":"14","author":"Wang","year":"2015","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1111\/1541-4337.12416","article-title":"High-amylose starches to bridge the \u201cfiber gap\u201d: Development, structure, and nutritional functionality","volume":"18","author":"Li","year":"2019","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"105388","DOI":"10.1016\/j.tifs.2025.105388","article-title":"A review on the comparison of preparation, structure and properties of two types of resistant starch type III formed from long and short-chain amylose","volume":"166","author":"Liu","year":"2025","journal-title":"Trends Food Sci. Technol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.jcs.2003.12.001","article-title":"Starch: Composition, fine structure and architecture","volume":"39","author":"Tester","year":"2004","journal-title":"J. Cereal Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"120185","DOI":"10.1016\/j.carbpol.2022.120185","article-title":"High-amylose maize starch: Structure, properties, modifications and industrial applications","volume":"299","author":"Obadi","year":"2023","journal-title":"Carbohydr. Polym."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"116681","DOI":"10.1016\/j.carbpol.2020.116681","article-title":"The relationship between the expression pattern of starch biosynthesis enzymes and molecular structure of high amylose maize starch","volume":"247","author":"Zhong","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.carbpol.2008.03.012","article-title":"Characterization of maize amylose-extender (ae) mutant starches. Part I: Relationship between resistant starch contents and molecular structures","volume":"74","author":"Li","year":"2008","journal-title":"Carbohydr. Polym."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"105820","DOI":"10.1016\/j.foodhyd.2020.105820","article-title":"High-amylose wheat and maize starches have distinctly different granule organization and annealing behaviour: A key role for chain mobility","volume":"105","author":"Li","year":"2020","journal-title":"Food Hydrocoll."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"109593","DOI":"10.1016\/j.foodhyd.2023.109593","article-title":"Characterization of different high amylose starch granules. Part II: Structure evolution during digestion and distinct digestion mechanisms","volume":"149","author":"Tian","year":"2024","journal-title":"Food Hydrocoll."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.carbpol.2014.08.091","article-title":"Rice starch granule amylolysis: Differentiating effects of particle size, morphology, thermal properties and crystalline polymorph","volume":"115","author":"Dhital","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.tifs.2015.01.004","article-title":"Densely packed matrices as rate determining features in starch hydrolysis","volume":"43","author":"Zhang","year":"2015","journal-title":"Trends Food Sci. Technol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.carbpol.2014.06.063","article-title":"Amylase binding to starch granules under hydrolysing and non-hydrolysing conditions","volume":"113","author":"Dhital","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1080\/10408398.2014.922043","article-title":"Mechanisms of starch digestion by \u03b1-amylase: Structural basis for kinetic properties","volume":"57","author":"Dhital","year":"2017","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1039\/C5FO01104C","article-title":"Intactness of cell wall structure controls the in vitro digestion of starch in legumes","volume":"7","author":"Dhital","year":"2016","journal-title":"Food Funct."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"127719","DOI":"10.1016\/j.foodchem.2020.127719","article-title":"Protein-starch matrix plays a key role in enzymic digestion of high-amylose wheat noodle","volume":"336","author":"Li","year":"2021","journal-title":"Food Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"107181","DOI":"10.1016\/j.foodhyd.2021.107181","article-title":"High-amylose wheat bread with reduced in vitro digestion rate and enhanced resistant starch content","volume":"123","author":"Li","year":"2022","journal-title":"Food Hydrocoll."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"108321","DOI":"10.1016\/j.foodhyd.2022.108321","article-title":"High-amylose wheat tortillas with reduced in vitro digestion and enhanced resistant starch content","volume":"137","author":"Li","year":"2023","journal-title":"Food Hydrocoll."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.foodhyd.2015.04.020","article-title":"Direct evidence for the non-additive gelatinization in binary starch blends: A case study on potato starch mixed with rice or maize starches","volume":"50","author":"Waterschoot","year":"2015","journal-title":"Food Hydrocoll."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1094\/CCHEM.1997.74.4.431","article-title":"Properties of some starch blends","volume":"74","author":"Obanni","year":"1997","journal-title":"Cereal Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"117736","DOI":"10.1016\/j.foodres.2025.117736","article-title":"Preserving resistant starch in retrograded maize starch gels: The role of microwave reheating power and target centre temperature","volume":"222","author":"Li","year":"2025","journal-title":"Food Res. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.carbpol.2014.08.001","article-title":"Exploring extraction\/dissolution procedures for analysis of starch chain-length distributions","volume":"114","author":"Wu","year":"2014","journal-title":"Carbohydr. Polym."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.foodhyd.2019.01.041","article-title":"Altering starch branching enzymes in wheat generates high-amylose starch with novel molecular structure and functional properties","volume":"92","author":"Li","year":"2019","journal-title":"Food Hydrocoll."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"116557","DOI":"10.1016\/j.carbpol.2020.116557","article-title":"High-amylose wheat starch: Structural basis for water absorption and pasting properties","volume":"245","author":"Li","year":"2020","journal-title":"Carbohydr. Polym."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1039\/C3FO60702J","article-title":"A standardised static in vitro digestion method suitable for food\u2014An international consensus","volume":"5","author":"Minekus","year":"2014","journal-title":"Food Funct."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.carbpol.2014.09.043","article-title":"The interplay of \u03b1-amylase and amyloglucosidase activities on the digestion of starch in in vitro enzymic systems","volume":"117","author":"Warren","year":"2015","journal-title":"Carbohydr. Polym."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"118367","DOI":"10.1016\/j.carbpol.2021.118367","article-title":"Ordered structural changes of retrograded starch gel over long-term storage in wet starch noodles","volume":"270","author":"Yang","year":"2021","journal-title":"Carbohydr. Polym."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1094\/CCHEM.1999.76.5.629","article-title":"Effects of amylopectin branch chain length and amylose content on the gelatinization and pasting properties of starch","volume":"76","author":"Jane","year":"1999","journal-title":"Cereal Chem."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"123633","DOI":"10.1016\/j.carbpol.2025.123633","article-title":"Characterization of different high amylose starch granules. Part III: How starch fine structures affect retrogradation and formation of type 3 resistant starch","volume":"361","author":"Ding","year":"2025","journal-title":"Carbohydr. Polym."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/S0008-6215(00)90086-7","article-title":"Crystallisation of malto-oligosaccharides as models of the crystalline forms of starch: Minimum chain-length requirement for the formation of double helices","volume":"161","author":"Gidley","year":"1987","journal-title":"Carbohydr. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0008-6215(92)85063-6","article-title":"Loss of crystalline and molecular order during starch gelatinisation: Origin of the enthalpic transition","volume":"227","author":"Cooke","year":"1992","journal-title":"Carbohydr. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.carbpol.2014.11.025","article-title":"Molecular, mesoscopic and microscopic structure evolution during amylase digestion of extruded maize and high amylose maize starches","volume":"118","author":"Shrestha","year":"2015","journal-title":"Carbohydr. Polym."}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/18\/3344\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:03:16Z","timestamp":1790002996000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/18\/3344"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,21]]},"references-count":32,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["foods15183344"],"URL":"https:\/\/doi.org\/10.3390\/foods15183344","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,21]]}}}