{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:00:42Z","timestamp":1792789242163,"version":"4.5.0"},"reference-count":24,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kasetsart University Institute for Advanced Studies","id":[{"id":"https:\/\/ror.org\/01mjdf856","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/01mjdf856","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N42A690553"],"award-info":[{"award-number":["N42A690553"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]},{"award":["N42A690553"],"award-info":[{"award-number":["N42A690553"]}],"id":[{"id":"https:\/\/ror.org\/018wfhg78","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Chiang Mai University","id":[{"id":"https:\/\/ror.org\/05m2fqn25","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/05m2fqn25","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Thailand\u2013United States Educational Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Foods"],"abstract":"<jats:p>This study systematically investigated how cassava starch modification chemistry and hydroxypropyl substitution level influence the structure and functional performance of extrusion-plasticized edible materials containing maltol or ethyl maltol. Native starch, acetylated starch, octenyl succinate-modified starch, and hydroxypropylated starches with low, medium, and high substitution levels were incorporated with 3% maltol or ethyl maltol and processed by twin-screw extrusion. The resulting materials were characterized for microstructure, molecular environment, crystalline organization, thermal behavior, mechanical properties, antioxidant activity, and antimicrobial performance. FTIR and NMR analyses showed changes in the molecular environment after incorporation of the maltol derivatives, while XRD revealed formulation-dependent differences in structural ordering. Mechanical responses were strongly matrix-dependent, with tensile strength, elongation at break, and Young\u2019s modulus reaching up to 3.56 MPa, 552%, and 108 MPa, respectively. DPPH radical-scavenging activity increased with extraction time and reached approximately 80\u201382% after 5 h in the most active formulations. Antibacterial activity was selective, with the largest inhibition zone against Bacillus subtilis reaching 3.53 mm, whereas no inhibition zones were detected against Staphylococcus aureus or Escherichia coli. Antifungal activity was more pronounced, with maximum inhibition zones of 7.39 mm against Aspergillus niger and 0.55 mm against Penicillium sp.; no distinct inhibition zone was observed against Rhizopus sp., although delayed sporulation was observed in some maltol-containing samples. These findings demonstrate that starch modification chemistry and substitution level govern the balance among structural organization, mechanical behavior, antioxidant activity, and selective antimicrobial performance of extrusion-plasticized cassava starch matrices containing maltol derivatives.<\/jats:p>","DOI":"10.3390\/foods15193393","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:10:31Z","timestamp":1790179831000},"page":"3393","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Flexible Edible Materials from Extrusion-Plasticized Modified Cassava Starches with Maltol and Ethyl Maltol: Molecular Interactions, Mechanical Properties, and Antioxidant and Antimicrobial Activities"],"prefix":"10.3390","volume":"15","author":[{"given":"Khwanchat","family":"Promhuad","sequence":"first","affiliation":[{"name":"Department of Packaging and Materials Technology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd., Latyao, Chatuchak, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4980-6542","authenticated-orcid":false,"given":"Phatthranit","family":"Klinmalai","sequence":"additional","affiliation":[{"name":"Faculty of Agro-Industry, Chiang Mai University, Samut Sakhon 74000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5602-3832","authenticated-orcid":false,"given":"Shyam S.","family":"Sablani","sequence":"additional","affiliation":[{"name":"Department of Biological System Engineering, Washington State University, Pullman, WA 99164, USA"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1787-5391","authenticated-orcid":false,"given":"Jong-Whan","family":"Rhim","sequence":"additional","affiliation":[{"name":"BioNanocomposite Research Center, Department of Food and Nutrition, Kyung Hee University, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6555-1637","authenticated-orcid":false,"given":"Nathdanai","family":"Harnkarnsujarit","sequence":"additional","affiliation":[{"name":"Department of Packaging and Materials Technology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Rd., Latyao, Chatuchak, Bangkok 10900, Thailand"},{"name":"Center for Advanced Studies for Agriculture and Food (CASAF), Kasetsart University Institute for Advanced Studies (KUIAS), Kasetsart University, 50 Ngam Wong Wan Rd., Latyao, Chatuchak, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Vilpoux, O.F., and Junior, J.F.S.S. (2023). Global production and use of starch. Starchy Crops Morphology, Extraction, Properties and Applications, Academic Press.","DOI":"10.1016\/B978-0-323-90058-4.00014-1"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Ye, J., Hu, X., Luo, S., Liu, W., Chen, J., Zeng, Z., and Liu, C. (2018). Properties of starch after extrusion: A review. Starch-St\u00e4rke, 70.","DOI":"10.1002\/star.201700110"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Promhuad, K., Bumbudsanpharoke, N., Wadaugsorn, K., Sonchaeng, U., and Harnkarnsujarit, N. (2022). Maltol-incorporated acetylated cassava starch films for shelf-life extension packaging of bakery products. Polymers, 14.","DOI":"10.3390\/polym14245342"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.carbpol.2016.07.009","article-title":"Effect of amylose content and nanoclay incorporation order in physicochemical properties of starch\/montmorillonite composites","volume":"152","year":"2016","journal-title":"Carbohydr. Polym."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1016\/j.ijbiomac.2022.12.194","article-title":"Extrusion-blown starch\/PBAT biodegradable active films incorporated with high retentions of tea polyphenols and the release kinetics into food simulants","volume":"227","author":"Zhai","year":"2023","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"682","DOI":"10.3390\/nu7010682","article-title":"Maltol, a food flavoring agent, attenuates acute alcohol-induced oxidative damage in mice","volume":"7","author":"Han","year":"2015","journal-title":"Nutrients"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ziklo, N., Bibi, M., and Salama, P. (2021). The antimicrobial mode of action of maltol and its synergistic efficacy with selected cationic surfactants. Cosmetics, 8.","DOI":"10.3390\/cosmetics8030086"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1111\/j.1472-765X.1996.tb01139.x","article-title":"Sensitization of Escherichia coli to nisin by maltol and ethyl maltol","volume":"22","author":"Schved","year":"1996","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1080\/10601325.2019.1578616","article-title":"Synthesis, characterization and antimicrobial activity of maltolMA functionalized chitosan derivatives","volume":"56","author":"Saud","year":"2019","journal-title":"J. Macromol. Sci. Part A"},{"key":"ref_10","unstructured":"(2010). Standard Test Method for Tensile Properties of Thin Plastic Sheeting (Standard No. ASTM D882-10)."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/s11694-022-01609-6","article-title":"Active edible coating based on guar gum with mint extract and antibrowning agents for ber (Ziziphus mauritiana) fruits preservation","volume":"17","author":"Kumar","year":"2023","journal-title":"J. Food Meas. Charact."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Promhuad, K., Papoompruk, M., Klinmalai, P., and Harnkarnsujarit, N. (2026). Characterization and Antimicrobial Activity of PLA-Laminated PBAT\/TPS Films Incorporated with Silver Nanocomposites. Foods, 15.","DOI":"10.3390\/foods15122132"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Cuenca, P., Ferrero, S., and Albani, O. (2020). Preparation and characterization of cassava starch acetate with high substitution degree. Food Hydrocoll., 100.","DOI":"10.1016\/j.foodhyd.2019.105430"},{"key":"ref_14","first-page":"150","article-title":"Formation of flavor, color, and reducing power during the production process of dark specialty malts","volume":"69","author":"Vandecan","year":"2011","journal-title":"J. Am. Soc. Brew. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.poly.2012.01.033","article-title":"Synthesis, crystal structures and spectroscopy studies of Mo(IV) complexes synthesized in reactions with kojic acid, maltol and ethylmaltol","volume":"37","author":"Szklarzewicz","year":"2012","journal-title":"Polyhedron"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"03014","DOI":"10.1051\/matecconf\/201819203014","article-title":"Tensile properties of PLA\/PBAT blends and PLA fibre-reinforced PBAT composite","volume":"Volume 192","author":"Sritham","year":"2018","journal-title":"MATEC Web of Conferences"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1094\/CCHEM.2003.80.3.290","article-title":"Effect of hydroxypropylation on retrogradation and water dynamics in wheat starch gels using 1H NMR","volume":"80","author":"Choi","year":"2003","journal-title":"Cereal Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.foodchem.2014.11.073","article-title":"Isolation of maltol glucoside from the floral nectar of New Zealand m\u0101nuka (Leptospermum scoparium)","volume":"174","author":"Adams","year":"2015","journal-title":"Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1002\/star.201200198","article-title":"Thermal degradation and stability of starch under different processing conditions","volume":"65","author":"Liu","year":"2013","journal-title":"Starch-St\u00e4rke"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1007\/s00396-020-04680-x","article-title":"Influence of surface roughness on contact angle hysteresis and spreading work","volume":"298","author":"Wang","year":"2020","journal-title":"Colloid Polym. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Xu, Y., Chen, X., Xu, X., Wang, B., Du, X., and Chen, P. (2020). Investigation on morphological structure and crystal transition of maize starch gelatinized in pure glycerol. J. Cereal Sci., 92.","DOI":"10.1016\/j.jcs.2020.102924"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.tca.2009.05.019","article-title":"Study on the morphology, crystalline structure and thermal properties of yellow ginger starch acetates with different degrees of substitution","volume":"495","author":"Zhang","year":"2009","journal-title":"Thermochim. Acta"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zhaojin, Y., Zhan, Z., Haiyang, W., Bo, W., Guangming, F., Ying, Z., and Xiaoming, J. (2024). Study on synthesis, pyrolysis and antioxidation of ethyl maltol succinate diester. Chin. J. Org. Chem., 44.","DOI":"10.6023\/cjoc202311011"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Promhuad, K., and Harnkarnsujarit, N. (2024). Volatile release antibacterial and antifungal biodegradable packaging: Effects of ethyl maltol and maltol blended polyester via cast-extrusion. Food Biosci., 62.","DOI":"10.1016\/j.fbio.2024.105116"}],"container-title":["Foods"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/19\/3393\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:21:08Z","timestamp":1790310068000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2304-8158\/15\/19\/3393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,23]]},"references-count":24,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["foods15193393"],"URL":"https:\/\/doi.org\/10.3390\/foods15193393","relation":{},"ISSN":["2304-8158"],"issn-type":[{"value":"2304-8158","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,23]]}}}